BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,643,049	G→A	22.6%	A341V (GCT→GTT)	accC ←	biotin carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,643,049	0	G	A	22.6%	213.3 / 56.4	106	A341V (GCT→GTT)	accC	biotin carboxylase
Reads supporting (aligned to +/- strand): ref base G (43/39); new base A (11/13); total (54/52)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 112 total aligned reads displayed.

CGCTACCATCGAATCATAATATGGCGGTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTACAGGATGTTCTACT  >  CP000730/1642910‑1643189

cGCTACCATCGAATCATAATATGGCGGTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGc                                                                                                                                             >  1:458872/1‑141 (MQ=255)

 gCTACCATCGAATCATAATATGGCGGTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCa                                                                                                                                            >  1:211937/1‑141 (MQ=255)

  cTACCATCGAATCATAATATGGCGGTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCAt                                                                                                                                           >  1:1007514/1‑141 (MQ=255)

   tACCATCGTATCATAATATGGCGGTATCGTATAATTAGTATAACATGCTGTCTCTATTCGAACATCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCAtt                                                                                                                                          <  2:1007630/141‑1 (MQ=255)

        tCGAATCATAATATGGCGGTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTc                                                                                                                                     <  1:328393/141‑1 (MQ=255)

        tCGAATCATAATATGGCGGTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTc                                                                                                                                     >  2:188222/1‑141 (MQ=255)

            aTCATAATATGGCGGTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTaaa                                                                                                                                 <  1:1176129/141‑1 (MQ=255)

                 aataTGGCGGTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCaa                                                                                                                            >  2:447647/1‑141 (MQ=255)

                   tatGGCGGTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAAtt                                                                                                                          <  2:233807/141‑1 (MQ=255)

                    atGGCGGTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTg                                                                                                                         >  2:407532/1‑141 (MQ=255)

                     tGGCGGTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGc                                                                                                                        <  2:1182470/141‑1 (MQ=255)

                     tGGCGGTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTCTAAATTCAATTGc                                                                                                                        <  2:654981/141‑1 (MQ=255)

                      ggCGGTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCg                                                                                                                       <  2:400038/141‑1 (MQ=255)

                      ggCGGTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTCTAAATTCAATTGCg                                                                                                                       >  2:914437/1‑141 (MQ=255)

                        cGGTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTCTAAATTCAATTGCgtg                                                                                                                     >  1:626152/1‑141 (MQ=255)

                         ggTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTCTAAATTCAATTGCgtgg                                                                                                                    >  2:393618/1‑140 (MQ=255)

                           tATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTcc                                                                                                                  >  1:1084005/1‑141 (MQ=255)

                           tATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTcc                                                                                                                  >  1:1121285/1‑141 (MQ=255)

                            aTCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCt                                                                                                                 <  1:104586/141‑1 (MQ=255)

                              cGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGt                                                                                                               <  2:245922/141‑1 (MQ=255)

                               gTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGtt                                                                                                              >  1:261724/1‑141 (MQ=255)

                                   aaTTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGttaatt                                                                                                          <  1:886624/141‑1 (MQ=255)

                                      tAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTCTAAATTCAATTGCGTGTCCTGttaatttaa                                                                                                       <  1:452404/141‑1 (MQ=255)

                                         tataACATGCTGACTCTATTCGTACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTCTAAATTCAAGTGCGTGTCCTGTTGATTTAatat                                                                                                    >  1:825354/1‑141 (MQ=255)

                                          ataACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATAtc                                                                                                   <  1:640286/141‑1 (MQ=255)

                                           taACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATAtct                                                                                                  >  1:1059679/1‑141 (MQ=255)

                                            aaCATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATAtctt                                                                                                 >  1:453933/1‑141 (MQ=255)

                                             aCATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTANTAtcttc                                                                                                >  2:895662/1‑141 (MQ=255)

                                              cATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATAtcttct                                                                                               >  1:518762/1‑141 (MQ=255)

                                                tGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTg                                                                                             <  2:583910/141‑1 (MQ=255)

                                                 gCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGt                                                                                            >  1:158823/1‑141 (MQ=255)

                                                  cTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGtt                                                                                           >  1:923208/1‑141 (MQ=255)

                                                   tGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGttt                                                                                          >  1:598016/1‑141 (MQ=255)

                                                   tGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGttt                                                                                          >  1:256901/1‑141 (MQ=255)

                                                      ctctATTCGAACACCATATCCACCTGTTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTtata                                                                                       <  2:34345/141‑1 (MQ=255)

                                                      ctctATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTtata                                                                                       >  2:864214/1‑141 (MQ=255)

                                                          aTTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGt                                                                                   >  2:708410/1‑141 (MQ=255)

                                                            tCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTaa                                                                                 >  2:636999/1‑141 (MQ=255)

                                                             cGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATNCGGTAac                                                                                >  2:896515/1‑141 (MQ=255)

                                                              gAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAaca                                                                               <  1:1159735/141‑1 (MQ=255)

                                                               aaCACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAacac                                                                              <  1:734624/141‑1 (MQ=255)

                                                               aaCACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAacac                                                                              <  2:271625/141‑1 (MQ=255)

                                                               aaCACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTCTAAATTCAATTGTGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAacac                                                                              <  1:37937/141‑1 (MQ=255)

                                                               aaCACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTCTAAATTCAATTGTGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAacac                                                                              <  1:695624/141‑1 (MQ=255)

                                                                acacCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACg                                                                             >  2:1037647/1‑141 (MQ=255)

                                                                acacCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACg                                                                             >  1:1000276/1‑141 (MQ=255)

                                                                acacCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACg                                                                             >  1:1135746/1‑141 (MQ=255)

                                                                acacCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACg                                                                             >  1:76392/1‑141 (MQ=255)

                                                                acacCATATCCACCGGGTGCAAGATATTGCTCGATTTTACCTTGTGATGGCATATAGTTCTTGTAAGGTTGTTCAGCATTAAGTCTAGATGCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACg                                                                             <  2:950266/141‑1 (MQ=255)

                                                                  acCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTc                                                                           >  2:635918/1‑141 (MQ=255)

                                                                           aCCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGc                                                                  <  1:82633/141‑1 (MQ=255)

                                                                            ccTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCa                                                                 <  1:1186144/141‑1 (MQ=255)

                                                                             cTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCaa                                                                <  2:186792/141‑1 (MQ=255)

                                                                              tGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAAc                                                               >  2:926022/1‑141 (MQ=255)

                                                                               ggTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACt                                                              >  1:874932/1‑141 (MQ=255)

                                                                                 tGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACtcg                                                            <  2:1173946/141‑3 (MQ=255)

                                                                                  gCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACttgt                                                           >  2:252730/1‑141 (MQ=255)

                                                                                    aaGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCCTAGCAACttgtaa                                                         >  2:620362/1‑141 (MQ=255)

                                                                                           tGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTtgtaattgtaat                                                  >  1:454048/1‑141 (MQ=255)

                                                                                             cTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAAttt                                                <  1:711944/141‑1 (MQ=255)

                                                                                             cTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAAttt                                                <  2:1046679/141‑1 (MQ=255)

                                                                                               cAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTaa                                              >  1:444942/1‑141 (MQ=255)

                                                                                                aaTTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAAc                                             <  2:39795/141‑1 (MQ=255)

                                                                                                aaTTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAAc                                             <  2:39815/141‑1 (MQ=255)

                                                                                                  ttttACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTa                                           <  2:211937/141‑1 (MQ=255)

                                                                                                  ttttACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTa                                           <  2:1007514/141‑1 (MQ=255)

                                                                                                   tttACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTaa                                          >  1:742557/1‑141 (MQ=255)

                                                                                                    ttACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTaaa                                         >  2:927579/1‑141 (MQ=255)

                                                                                                    ttACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTaaa                                         <  1:518561/141‑1 (MQ=255)

                                                                                                    ttACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTaaa                                         <  1:1123981/141‑1 (MQ=255)

                                                                                                      aCCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATc                                       >  2:110525/1‑141 (MQ=255)

                                                                                                               tGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGt                              <  1:525652/141‑1 (MQ=255)

                                                                                                               tGGCATAAAGTTCTTGTAAGGATTTTCAACATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGt                              <  2:282409/141‑1 (MQ=255)

                                                                                                                ggCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCATCGTGTAATTGTAATTTAACTAAATCAATTCCTGtt                             <  1:154788/141‑1 (MQ=255)

                                                                                                                ggCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGtt                             <  2:40390/141‑1 (MQ=255)

                                                                                                                ggCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGtt                             <  1:301902/141‑1 (MQ=255)

                                                                                                                 gCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTa                            >  2:84873/1‑141 (MQ=255)

                                                                                                                 gCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTa                            <  1:1080095/141‑1 (MQ=255)

                                                                                                                   aTAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTg                               <  2:648149/136‑1 (MQ=255)

                                                                                                                   aTAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATAAGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTg                               >  1:648149/1‑136 (MQ=255)

                                                                                                                       aGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCAttt                      >  1:1056778/1‑141 (MQ=255)

                                                                                                                            ttGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGtt                 >  1:573802/1‑141 (MQ=255)

                                                                                                                            ttGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGtt                 >  2:725710/1‑141 (MQ=255)

                                                                                                                             tGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTa                <  2:1059679/141‑1 (MQ=255)

                                                                                                                              gTAAGGATTTTCAACATTACTTCTAAATTCAATTGCGGGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTNAACTAAATCAATTCCTGTTACCATTTCAGTTAc               <  1:1003532/141‑1 (MQ=255)

                                                                                                                              gTAAGGATTTTCAACATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTAc               <  1:839509/141‑1 (MQ=255)

                                                                                                                                 aGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTACAgg            <  1:705033/141‑1 (MQ=255)

                                                                                                                                 aGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTACAgg            >  1:451997/1‑141 (MQ=255)

                                                                                                                                 aGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTACAgg            <  1:178616/141‑1 (MQ=255)

                                                                                                                                 aGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTACAgg            >  2:435658/1‑141 (MQ=255)

                                                                                                                                  ggATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTACAGGa           <  1:204005/141‑1 (MQ=255)

                                                                                                                                   gATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTACAGGAt          <  1:817044/141‑1 (MQ=255)

                                                                                                                                   gATTTTCAACATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTACAGGAt          <  2:256901/141‑1 (MQ=255)

                                                                                                                                    aTTTTCAACATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTACAGGATg         >  1:680555/1‑141 (MQ=255)

                                                                                                                                         cAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTACAGGATGTTCTa    >  1:412648/1‑141 (MQ=255)

                                                                                                                                           gCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTGACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTACAGGATGTTCTACt  >  1:423396/1‑141 (MQ=255)

                                                                                                                                           gCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTACAGGATGTTCTACt  >  1:751633/1‑141 (MQ=255)

                                                                                                                                           gCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTACAGGATGTTCTACt  >  2:45049/1‑141 (MQ=255)

                                                                                                                                           gCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGATTTTACGGTAACACGTCACCCATAGCTGCTTGTGGAAGTAATGAAACTAAATCAATTCCTGTTACCATTTCAGTTACAGGATGTTCTACt  >  1:1129185/1‑141 (MQ=255)

                                                                                                                                           gCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATATTCTTGTTTATACGGTAACACGTATTAAAAAGCATCGTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTACAGGATGTTCTACt  <  1:1009333/141‑1 (MQ=255)

CGCTACCATCGAATCATAATATGGCGGTATCGTATAATTAGTATAACATGCTGACTCTATTCGAACACCATATCCACCTGGTGCAAGATATTGCTCAATTTTACCTGGTGATGGCATAAAGTTCTTGTAAGGATTTTCAGCATTAATTCTAAATTCAATTGCGTGTCCTGTTAATTTAATATCTTCTTGTTTATACGGTAACACGTCACCCATAGCAACTTGTAATTGTAATTTAACTAAATCAATTCCTGTTACCATTTCAGTTACAGGATGTTCTACT  >  CP000730/1642910‑1643189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`