Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,283,586 A→T 5.3% intergenic (+5254/+2778) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,283,5860AT 5.3% 386.5 / 2.2 122intergenic (+5254/+2778)mtlD/glmMmannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base A (44/68);  new base T (7/2);  total (52/70)
Fisher's exact test for biased strand distribution p-value = 3.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

TGCTTTTGAGCTGCATCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAGCTGCATTAATACGTTCAATAGCTTCCGCTACTTCT  >  CP000730/2283449‑2283724
                                                                                                                                         |                                                                                                                                          
tGCTTTTGAGCTGCATCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTATGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTagag                                                                                                                                         >  1:452190/1‑141 (MQ=255)
       gAGCTGCATCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTGACTTCATTATAGGCATCCATTTTTGGTTGCGTAGAGACGGTac                                                                                                                                  >  1:588926/1‑140 (MQ=255)
       gAGCTGCATCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTaa                                                                                                                                  <  1:718520/141‑1 (MQ=255)
       gAGCTGCATCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTaa                                                                                                                                  >  2:505480/1‑141 (MQ=255)
        aGCTGCATCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAat                                                                                                                                 >  1:1015966/1‑141 (MQ=255)
        aGCTGCATCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAat                                                                                                                                 <  2:856911/141‑1 (MQ=255)
            gCATCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTTTTTGCGTTTTTTCAGTAATAttt                                                                                                                             >  2:192568/1‑141 (MQ=255)
            gCATCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATAttt                                                                                                                             >  2:845293/1‑141 (MQ=255)
            gCATCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATGTGGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATAttt                                                                                                                             <  1:621539/141‑1 (MQ=255)
             cATCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATAtttt                                                                                                                            <  2:949121/141‑1 (MQ=255)
             cATCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATAtttt                                                                                                                            >  2:447583/1‑141 (MQ=255)
             cATCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATAtttt                                                                                                                            <  1:1078413/141‑1 (MQ=255)
              aTCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGACACTGTTGCATTTTCAGCTTTACTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTc                                                                                                                           <  1:596694/141‑1 (MQ=255)
              aTCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTc                                                                                                                           <  1:875518/141‑1 (MQ=255)
              aTCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTc                                                                                                                           <  2:1159425/141‑1 (MQ=255)
              aTCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTc                                                                                                                           <  2:937747/141‑1 (MQ=255)
              aTCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTc                                                                                                                           <  1:980351/141‑1 (MQ=255)
              aTCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTc                                                                                                                           <  1:954335/141‑1 (MQ=255)
                  aCTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAAtt                                                                                                                       >  1:333175/1‑141 (MQ=255)
                     gctgcATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAAttttt                                                                                                                    >  1:833258/1‑141 (MQ=255)
                        gcATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCGGTAATATGTTCATTTTTTggg                                                                                                                 >  2:412265/1‑139 (MQ=255)
                         cATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTgaga                                                                                                                <  2:755723/141‑1 (MQ=255)
                           tCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGAtt                                                                                                              <  1:832848/141‑1 (MQ=255)
                            cAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGAttt                                                                                                             >  1:790080/1‑141 (MQ=255)
                             aGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATttc                                                                                                            >  2:757/1‑141 (MQ=255)
                              gCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATttct                                                                                                           <  1:68616/141‑1 (MQ=255)
                               cTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATttctt                                                                                                          >  2:484816/1‑141 (MQ=255)
                                ttCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATttcttc                                                                                                         <  1:52598/141‑1 (MQ=255)
                                  cTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGATACAGTAATATTTTCAATTTTTGAGATTTCTTCGt                                                                                                       >  2:23922/1‑141 (MQ=255)
                                  cTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGt                                                                                                       >  1:869140/1‑141 (MQ=255)
                                  cTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGt                                                                                                       >  1:226788/1‑141 (MQ=255)
                                  cTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGt                                                                                                       >  2:23860/1‑141 (MQ=255)
                                    gCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGttt                                                                                                     >  1:514801/1‑141 (MQ=255)
                                     cTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGtttt                                                                                                    <  1:533810/141‑1 (MQ=255)
                                     cTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGtttt                                                                                                    >  1:1177807/1‑141 (MQ=255)
                                       aCTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTa                                                                                                  <  2:491335/141‑1 (MQ=255)
                                         ttcttcATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAAc                                                                                                <  1:785701/141‑1 (MQ=255)
                                            ttcATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAActct                                                                                             <  1:746613/141‑1 (MQ=255)
                                            ttcATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAActct                                                                                             <  1:931850/141‑1 (MQ=255)
                                            ttcATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAActct                                                                                             <  1:235638/141‑1 (MQ=255)
                                              cATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCttt                                                                                           <  2:1115224/141‑1 (MQ=255)
                                               aTTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTCGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTc                                                                                          >  2:559994/1‑141 (MQ=255)
                                                tttGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCt                                                                                         <  2:317235/141‑1 (MQ=255)
                                                 ttgttgCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTa                                                                                        <  1:63821/141‑1 (MQ=255)
                                                 cagttgCATTTGAAACTGTAGCATTTTGCGCTTTTCTAGCTGTAGCTGCTTGTCTAACTTCTTTATAAGCATCCATTTTAGTTTGTGTTGAATCCGTAATATTCTCAATTTTGAATATTTCTTCGTTTTTAACGCGTTCTa                                                                                        <  2:436356/139‑1 (MQ=255)
                                                     tgCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATc                                                                                    >  2:1052805/1‑141 (MQ=255)
                                                      gCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCt                                                                                   >  1:1135464/1‑141 (MQ=255)
                                                       cATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTTGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCtt                                                                                  >  2:707706/1‑141 (MQ=255)
                                                        aTTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTtg                                                                                 <  1:643218/141‑1 (MQ=255)
                                                         tttGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGTTTTCTTCGTTTTTAAATCTTTCTAAATCTtgt                                                                                >  2:1147319/1‑141 (MQ=255)
                                                           tGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGc                                                                              <  2:977887/141‑1 (MQ=255)
                                                                cTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCg                                                                         >  2:883858/1‑141 (MQ=255)
                                                                 tGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGt                                                                        >  1:179377/1‑141 (MQ=255)
                                                                  gTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGtt                                                                       >  1:822470/1‑141 (MQ=255)
                                                                  gTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGtt                                                                       >  1:1157001/1‑141 (MQ=255)
                                                                   tAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTg                                                                      <  1:883858/141‑1 (MQ=255)
                                                                    aGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGt                                                                     <  1:1022331/141‑1 (MQ=255)
                                                                      cATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCg                                                                   <  1:484816/141‑1 (MQ=255)
                                                                        ttttGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCt                                                                 <  1:876639/141‑1 (MQ=255)
                                                                          ttGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTc                                                               >  1:444608/1‑141 (MQ=255)
                                                                          ttGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACCTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGCTGTCGCTTc                                                               <  2:537356/141‑1 (MQ=255)
                                                                            gAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCaa                                                             <  2:1161307/141‑1 (MQ=255)
                                                                            gAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCaa                                                             <  1:922039/141‑1 (MQ=255)
                                                                             aGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAAt                                                            <  2:105078/141‑1 (MQ=255)
                                                                               cTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTg                                                          <  1:893136/141‑1 (MQ=255)
                                                                                     tAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTaa                                                    <  2:790080/141‑1 (MQ=255)
                                                                                     tAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTaa                                                    <  2:219707/141‑1 (MQ=255)
                                                                                        cTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAacac                                                 >  1:137414/1‑141 (MQ=255)
                                                                                        cTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAACCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAacac                                                 <  2:1010142/141‑1 (MQ=255)
                                                                                         tGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACAcc                                                <  1:890999/141‑1 (MQ=255)
                                                                                           ttGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAg                                              <  1:526242/141‑1 (MQ=255)
                                                                                           ttGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAg                                              <  1:591211/141‑1 (MQ=255)
                                                                                            tGCAGCTTGTTTCATATTATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGa                                             <  1:664416/141‑1 (MQ=255)
                                                                                            tGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGa                                             >  1:305618/1‑141 (MQ=255)
                                                                                             gCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGaa                                            <  2:319482/141‑1 (MQ=255)
                                                                                               tgCTTGTCTAACTTCTTTATAAGCATCCATTTTAGTTTGAGTTGAATCCGTAATATTCTCAATTTTGAATATTTCTTCGTTTTTAACGCGTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAAc                                          <  1:878959/140‑1 (MQ=255)
                                                                                               aGCTTGTTTAACTTCATTATAGGCATCCATTTTTTTTTTTTTTTTGTCAGTAATATTTTCAATTTTTGTGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAAc                                          >  2:963727/1‑141 (MQ=255)
                                                                                               aGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAAc                                          <  1:658860/141‑1 (MQ=255)
                                                                                               aGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAAc                                          >  1:760925/1‑141 (MQ=255)
                                                                                                   tGTTTAACTTCATTATAGGCATCCATTTTTTTTTTTTTTTTGTCAATAATATGTGCAATTCTTGAGATTTCTGCGGTTTTAGCTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTGCTATGGTTTTTACACCAAAAACTTTa                                      >  2:202493/1‑141 (MQ=255)
                                                                                                    ttttAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCGATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCTGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAg                                     <  2:373541/140‑1 (MQ=255)
                                                                                                     tttAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAGc                                    <  1:869006/141‑1 (MQ=255)
                                                                                                         aCTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAGCTGCa                                <  1:649410/141‑1 (MQ=255)
                                                                                                          cTTCATTATAGGCATCCATTTTTGTTTTCGTAGTGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAACCTTTCGCTGcct                               >  2:1061551/1‑139 (MQ=255)
                                                                                                           ttCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAGCTGCAtt                              >  2:369077/1‑141 (MQ=255)
                                                                                                             cATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAGCTGCATTaa                            <  1:734024/141‑1 (MQ=255)
                                                                                                             cATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAGCTGCATTaa                            <  1:361014/141‑1 (MQ=255)
                                                                                                             cATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTCCAATTTTTGATATTTCCTCGTCTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAGCTGCATTaa                            <  1:893912/141‑1 (MQ=255)
                                                                                                              aTTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATttctt                                                                                                          <  1:355323/62‑1 (MQ=255)
                                                                                                              aTTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAAGATTTTCAATTTTTGAGATttctt                                                                                                          >  2:355323/1‑62 (MQ=255)
                                                                                                                   aGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAGCTGCATTAATACGtt                      <  2:2976/141‑1 (MQ=255)
                                                                                                                    ggCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAGCTGCATTAATACGTTc                     >  2:750388/1‑141 (MQ=255)
                                                                                                                    ggCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAGCTGCATTAATACGTTc                     >  1:153355/1‑141 (MQ=255)
                                                                                                                       aTCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAACCTTTAGCTGCATTAATACGTTCAAt                  <  2:6690/141‑1 (MQ=255)
                                                                                                                       aTCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAGCTGCATTAATACGTTCAAt                  <  1:1095041/141‑1 (MQ=255)
                                                                                                                                tgtttgCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAGCTGCATTAATACGTTCAATAGCTTCCGc         >  1:490922/1‑141 (MQ=255)
                                                                                                                                  tttgCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAGCTGCATTAATACGTTCAATAGCTTCCGCTa       <  2:284512/141‑1 (MQ=255)
                                                                                                                                    tgCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAGCTGCATTAATACGTTCAATAGCTTCCGCTAct     <  2:833258/141‑1 (MQ=255)
                                                                                                                                      cGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAGCTGCATTAATACGTTCAATAGCTTCCGCTActtc   <  1:119309/141‑1 (MQ=255)
                                                                                                                                       gTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTGAACACCAGAAACTTTAGCTGCATTAATACGTTCAATAGCTTCCGCTActtct  <  2:1007826/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
TGCTTTTGAGCTGCATCTACTGCTGCATCAGCTTCTGCTACTTCTTCATTTGTTGCATTTGAAACTGTAGCATTTTGAGCTTTTCTAGCTGTTGCAGCTTGTTTAACTTCATTATAGGCATCCATTTTTGTTTGCGTAGAGTCAGTAATATTTTCAATTTTTGAGATTTCTTCGTTTTTAACTCTTTCTAAATCTTGTGCAGTCGTTGTCGCTTCAATTGCTTTAACACCAGAAACTTTAGCTGCATTAATACGTTCAATAGCTTCCGCTACTTCT  >  CP000730/2283449‑2283724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: