Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,775,029 G→A 6.3% D719D (GAC→GAT clfB ← clumping factor B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,775,0290GA 6.3% 219.7 / 2.6 75D719D (GAC→GATclfBclumping factor B
Reads supporting (aligned to +/- strand):  ref base G (27/43);  new base A (2/3);  total (29/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 118 total aligned reads displayed.

GAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATC  >  CP000730/2774889‑2775169
                                                                                                                                            |                                                                                                                                            
      gAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGtctgagtcgctgtctgagtctgagtcgctgtctgag                                                                                                                                        >  1:629770/1‑141 (MQ=255)
      gAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGtctgagtcgctgtctgagtctgagtcgctgtctgag                                                                                                                                        >  2:1079721/1‑141 (MQ=255)
        gTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCtgagtcgctgtctgagtctgagtcgctgtctgagtc                                                                                                                                      <  1:405347/141‑1 (MQ=255)
              gTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATc                                                                                                                                <  1:266069/141‑1 (MQ=255)
              gTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATc                                                                                                                                >  1:727120/1‑141 (MQ=255)
                    atctgagtcGCTATCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                                                                          <  2:455564/141‑1 (MQ=11)
                    atctgaatcGCTGTCTGTGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTTAGTCTGAGTCGCTATCTGAATCTGAATCGCCATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                                                                          <  2:543521/141‑1 (MQ=14)
                    atctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                                                                          <  1:131517/141‑1 (MQ=255)
                       tgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctga                                                                                                                       <  2:1130108/141‑1 (MQ=255)
                       tgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctga                                                                                                                       >  1:786065/1‑141 (MQ=255)
                       tgaatcGCTGTCTGAGGCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCCGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctga                                                                                                                       <  1:662027/141‑1 (MQ=255)
                          ctcactttCTGAGTCCGAATCGCTATCTGACTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc                                                                                                                    <  2:501102/134‑1 (MQ=14)
                            cGCTGTCTGGGTCAGAATCTCTATCTGAAGCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctg                                                                                                                  <  2:48794/141‑1 (MQ=17)
                              cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                                                                                                >  1:211082/1‑141 (MQ=255)
                                gTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCtgagtctgagtc                                                                                                              >  2:1133669/1‑141 (MQ=255)
                                gTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                                                                              <  1:797750/141‑1 (MQ=255)
                                gTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                                                                              <  1:605538/141‑1 (MQ=255)
                                      ggctgactCGCTAGCTGGAGCTGAGTCGCTGTCTGAGGCTGAGTCGCTATCTGGATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCGGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCGGAGTCGCGgtc                                                                                                        <  2:240813/134‑1 (MQ=14)
                                        ccGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctg                                                                                                      <  1:523966/141‑1 (MQ=255)
                                         cGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTatctga                                                                                                     >  1:785966/1‑141 (MQ=255)
                                            gtCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTgtctgagtc                                                                                                  <  2:232150/140‑1 (MQ=255)
                                            aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTgtctgagtc                                                                                                  >  2:725095/1‑141 (MQ=255)
                                               gCTATCTGAGTCTGAGTCGCTCTCTGAGTCTGAATCGCTATCTGAATCTGAGTCTCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCACTATCTGAGTCTGAGTCGCTgtctgagtctga                                                                                               <  2:331320/141‑1 (MQ=11)
                                                ctgtctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                                                                              <  2:621943/138‑1 (MQ=255)
                                                cTATCTGATTCTGAGTCGCTTTCTGAATCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                                                                              <  2:922765/141‑1 (MQ=255)
                                                cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGTATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                                                                              <  1:939439/141‑1 (MQ=255)
                                                   tctgagtctgagTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                                                                                           <  2:1049312/141‑1 (MQ=255)
                                                   tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                                                                                           >  2:1165177/1‑141 (MQ=255)
                                                   tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                                                                                           >  2:282828/1‑141 (MQ=255)
                                                   tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                                                                                           <  1:558671/141‑1 (MQ=255)
                                                      gagtctgaGTCGCTGTCCGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctg                                                                                        <  2:484578/138‑1 (MQ=14)
                                                      gaatctgaGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctg                                                                                        <  2:484579/141‑1 (MQ=255)
                                                        atctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtc                                                                                      <  1:94026/141‑1 (MQ=255)
                                                         tctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtct                                                                                     >  1:614131/1‑141 (MQ=255)
                                                         tctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtct                                                                                     >  2:923965/1‑141 (MQ=255)
                                                         tctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTCTCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtct                                                                                     >  2:774862/1‑141 (MQ=255)
                                                             aGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaat                                                                                 >  1:1161462/1‑141 (MQ=255)
                                                             aGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaat                                                                                 <  2:90920/141‑1 (MQ=255)
                                                              gTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatc                                                                                <  1:136969/141‑1 (MQ=255)
                                                              gTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCGGAGTCGCTATCGGAGtctgagtcgcggtctgaatc                                                                                >  2:736156/1‑141 (MQ=255)
                                                                cGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCACAGCCTGAGTCTGAATCGCTAACTGACTCTGACTCGCTATCTGAGtctgtgtcgccttctgcatctg                                                                              >  1:24866/1‑141 (MQ=14)
                                                                   tatctgagtcggaATCGCTATCTGAGTCGGAATCACTGTCGGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGtgtgagtcgctgtatgagtctgagt                                                                           >  2:772899/3‑141 (MQ=11)
                                                                     tctgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcg                                                                         <  1:816512/141‑1 (MQ=255)
                                                                     tctgagtccgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcg                                                                         <  2:786710/141‑1 (MQ=255)
                                                                        gagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctg                                                                      <  1:962991/141‑1 (MQ=255)
                                                                        gagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctg                                                                      <  1:175904/141‑1 (MQ=255)
                                                                           tctgaGTCGCTATCTGAATCTGAGTCACTCTCTGAGTCTGAGTCGCTCTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGACTCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtct                                                                   <  2:1004162/141‑1 (MQ=11)
                                                                           tctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtct                                                                   >  1:1094188/1‑141 (MQ=255)
                                                                            ctgaTTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctatctg                                                                  <  2:731895/141‑1 (MQ=11)
                                                                                aTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCtgagtcgctgtctgaatctgagtcgctgtctgaatc                                                              >  1:942217/1‑141 (MQ=255)
                                                                                aTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCtgagtcgctgtctgaatctgagtcgctgtctgaatc                                                              >  2:279539/1‑141 (MQ=255)
                                                                                aTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCtgagtcgctgtctgaatctgagtcgctgtctgaatc                                                              >  1:854619/1‑141 (MQ=255)
                                                                                  cGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGGTGTCTGAGTCTGcgtcggtgtgggaatctgagtcgctctctgagtctg                                                            >  2:823320/1‑141 (MQ=11)
                                                                                  cGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGagtcgctgtctgaatctgagtcgctgtctgaatctg                                                            <  1:973414/141‑1 (MQ=255)
                                                                                    cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGtctgaatctgaa                                                          <  2:268641/141‑1 (MQ=255)
                                                                                       tctgaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtctttcatatttata                                                       >  1:988593/1‑129 (MQ=255)
                                                                                         tgaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCt                                                     <  2:1161462/141‑1 (MQ=255)
                                                                                          gaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTg                                                    >  1:696720/1‑141 (MQ=255)
                                                                                              ctgaGTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctg                                                <  2:776786/141‑1 (MQ=255)
                                                                                              ctgaGTCGCTATCTGAGTCTGACTCGATGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctg                                                <  1:433148/141‑1 (MQ=255)
                                                                                                gaGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTC‑‑AG‑‑‑‑TGTCTGAGTCTGAATCGCTGTCTGTGTCTGAGTCGGTGTCCGAGTCTGAGTCGCTGTCTGAGTCTGAGTCAGTGTCTGAATCTGAATCGGTAtctgaatctgaa                                        >  2:797750/1‑141 (MQ=0)
                                                                                                 aGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGTATCTGAATCGCTgtctgtgt                                             >  2:457958/1‑141 (MQ=255)
                                                                                                     gCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgagtctga                                         >  1:190229/1‑141 (MQ=255)
                                                                                                      gcaatgcagtctgagtcggtgtatgagtctgcgtcgctgtctgAGACTGATTCGATATCTATATCTGCGCCGCCCCCTGATTCTTAGTCTTTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaa                                        <  1:713474/134‑1 (MQ=9)
                                                                                                      cTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaa                                        <  2:224186/141‑1 (MQ=255)
                                                                                                      cTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaa                                        <  1:404973/141‑1 (MQ=255)
                                                                                                        aTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatc                                      <  1:141314/141‑1 (MQ=255)
                                                                                                         tctgagtctgagtcgctgtctgagtctgagtcgctgTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcg                                     >  1:572541/1‑141 (MQ=255)
                                                                                                           tgagtctgagtcgctgtctgagtctgagtcgctgtcTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGATTCTGAGTCGCTGtctgaatctgaatcgct                                   <  1:566550/141‑1 (MQ=14)
                                                                                                            gagtctgagtcgctggctgagtctgagtcgctgtctGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgcta                                  <  1:990850/141‑1 (MQ=255)
                                                                                                                ctgagtcgctgtctgagtctgagtcgctgtctgagtCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctg                              <  2:969788/141‑1 (MQ=255)
                                                                                                                 tgagtcgctgtctgagtctgagtcgctgtctgagtcTGAATCGCTATCTGACTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctga                             <  2:669823/141‑1 (MQ=255)
                                                                                                                 tgagtcgcgctctgagtctgagtcgctgtctgagtcTGAATCGCTATCTGACTCTGAGTCGCTGTCTGACTCTGAGTCGCCGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgattctgaatcgctatctga                             <  2:708279/141‑1 (MQ=17)
                                                                                                                  gagtcgctgtctgagtctgagtcgctgtctgagtctGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgaatctgaatcgctatctgag                            <  1:624649/141‑1 (MQ=255)
                                                                                                                    gtcgctgtctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTg                                                    >  2:959179/1‑115 (MQ=255)
                                                                                                                    gtcgctgtctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTg                                                    <  1:959179/115‑1 (MQ=255)
                                                                                                                    gtcgctgtctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtc                          >  2:563122/1‑141 (MQ=255)
                                                                                                                    gtcgctgtctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgaatctgaatcgctatctgagtc                          >  2:382977/1‑141 (MQ=255)
                                                                                                                        ctgtctgagtctgagtcgctgtctgagtctgaATCGCTatctga                                                                                                                       >  2:1154677/1‑44 (MQ=40)
                                                                                                                          gtctgagtctgagtcgctgtctgcgtccgaGTCGCTAACCGAATACGAGACGCCATACGTGTCCGCGTCCCAGCCGGAACCGCAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatc                    <  1:113361/141‑1 (MQ=9)
                                                                                                                            ctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatcgc                  >  1:561010/1‑141 (MQ=255)
                                                                                                                             tgagtctgagtcgctgtctgagtctgaCTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGtctgagtctgaatcgctatctgagtctgaatcgct                 <  2:267518/141‑1 (MQ=255)
                                                                                                                             tgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatcgct                 >  1:52633/1‑141 (MQ=255)
                                                                                                                              gagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatcgcta                >  1:615732/1‑141 (MQ=255)
                                                                                                                                gtctgagtcgctgtctgagtctgaCTCGCTATCTGCATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCtgagtctgaatcgctatctgagtctgaatcgctatc              <  2:1044292/141‑1 (MQ=255)
                                                                                                                                gtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCtgagtctgaatcgctatctgagtctgaatcgctatc              <  2:547926/141‑1 (MQ=255)
                                                                                                                                 tctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                                                                              <  1:235954/60‑1 (MQ=38)
                                                                                                                                  ctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGagtctgaatcgctatctgagtctgaatcgctatctg            >  1:404589/1‑141 (MQ=255)
                                                                                                                                  ctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGagtctgaatcgctatctgaatctgaatcgctatctg            >  2:337995/1‑141 (MQ=255)
                                                                                                                                   tgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAgtctgaatcgctatctgagtctgaatcgctatctga           <  2:433983/141‑1 (MQ=255)
                                                                                                                                    gagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGtctgaatcgctatctgagtctgaatcgctatctgag          <  1:416569/141‑1 (MQ=255)
                                                                                                                                      gtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCtgaatcgctatctgagtctgaatcgctatctgagtc        <  1:695197/141‑1 (MQ=255)
                                                                                                                                       tcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTgaatcgctatctgagtctgaatcgctatctgagtct       >  2:4186/1‑141 (MQ=255)
                                                                                                                                         gctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAatcgctatctgagtctgaatcgctatctgagtctga     >  1:681108/1‑141 (MQ=255)
                                                                                                                                         gctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAACCTGAGTCGCTGTCTGAATGTGAATCGCTGtctgagtctgaagcgctatctgaatctgagtcggtgggggagtccga     >  2:162405/1‑127 (MQ=14)
                                                                                                                                          ctgtctgagtctgaATCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGATTCTGACTCGCTGTCTGAGTCTGAAtcgctatctgagtctgaatcgctatctgagtctgaa    <  2:1028479/141‑1 (MQ=255)
                                                                                                                                          ctgtctgagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAAtcgctatctgagtctgaatcgctatctgagtctgaa    <  2:572541/141‑1 (MQ=255)
                                                                                                                                          ctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAAtcgctatctgagtctgaatcgctatctgagtctgaa    <  2:1117531/141‑1 (MQ=255)
                                                                                                                                            gtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGACTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAGTCgctatctgagtctgaatcgctatctgagtctgaatc  <  2:564326/141‑1 (MQ=255)
                                                                                                                                            gtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCgctatctgagtctgaatcgctatctgagtctgaatc  <  1:895525/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATC  >  CP000730/2774889‑2775169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: