BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,775,053	A→G	6.1%	D711D (GAT→GAC)	clfB ←	clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,775,053	0	A	G	6.1%	285.0 / 4.4	113	D711D (GAT→GAC)	clfB	clumping factor B
Reads supporting (aligned to +/- strand): ref base A (46/54); new base G (2/5); total (50/63)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

AATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCACTGTC  >  CP000730/2774914‑2775193

aatcGCTGTCTGGGTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                                                                                             <  2:765618/141‑1 (MQ=14)

 ctcactttCTGAGTCCGAATCGCTATCTGACTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc                                                                                                                                            <  2:501102/134‑1 (MQ=14)

   cGCTGTCTGGGTCAGAATCTCTATCTGAAGCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctg                                                                                                                                          <  2:48794/141‑1 (MQ=17)

     cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                                                                                                                        >  1:211082/1‑141 (MQ=255)

       gTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCtgagtctgagtc                                                                                                                                      >  2:1133669/1‑141 (MQ=255)

       gTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                                                                                                      <  1:797750/141‑1 (MQ=255)

        tCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCg                                                                                                                                     >  1:583108/1‑141 (MQ=255)

           gAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTg                                                                                                                                  <  1:513004/141‑1 (MQ=255)

               ccGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctg                                                                                                                              <  1:523966/141‑1 (MQ=255)

                cGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTatctga                                                                                                                             >  1:785966/1‑141 (MQ=255)

                   gtCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTgtctgagtc                                                                                                                          <  2:232150/140‑1 (MQ=255)

                   aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTgtctgagtc                                                                                                                          >  2:725095/1‑141 (MQ=255)

                      gCTATCTGAGTCTGAGTCGCTCTCTGAGTCTGAATCGCTATCTGAATCTGAGTCTCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCACTATCTGAGTCTGAGTCGCTgtctgagtctga                                                                                                                       <  2:331320/141‑1 (MQ=11)

                       ctgtctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                                                                                                      <  2:621943/138‑1 (MQ=255)

                       cTATCTGATTCTGAGTCGCTTTCTGAATCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                                                                                                      <  2:922765/141‑1 (MQ=255)

                       cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                                                                                                      <  1:301980/141‑1 (MQ=255)

                          tctgagtctgagTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                                                                                                                   <  2:1049312/141‑1 (MQ=255)

                          tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                                                                                                                   >  2:1165177/1‑141 (MQ=255)

                             gagtctgaGTCGCTGTCCGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctg                                                                                                                <  2:484578/138‑1 (MQ=14)

                               atctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtc                                                                                                              <  1:94026/141‑1 (MQ=255)

                                tctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtct                                                                                                             >  1:614131/1‑141 (MQ=255)

                                    aGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaat                                                                                                         <  2:90920/141‑1 (MQ=255)

                                     gTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatc                                                                                                        <  1:136969/141‑1 (MQ=255)

                                     gTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCGGAGTCGCTATCGGAGtctgagtcgcggtctgaatc                                                                                                        >  2:736156/1‑141 (MQ=255)

                                       cGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCACAGCCTGAGTCTGAATCGCTAACTGACTCTGACTCGCTATCTGAGtctgtgtcgccttctgcatctg                                                                                                      >  1:24866/1‑141 (MQ=14)

                                          tatctgagtcggaATCGCTATCTGAGTCGGAATCACTGTCGGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGtgtgagtcgctgtatgagtctgagt                                                                                                   >  2:772899/3‑141 (MQ=11)

                                            tctgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcg                                                                                                 <  1:816512/141‑1 (MQ=255)

                                            tctgagtccgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcg                                                                                                 <  2:786710/141‑1 (MQ=255)

                                               gagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctg                                                                                              <  1:962991/141‑1 (MQ=255)

                                                  tctgaGTCGCTATCTGAATCTGAGTCACTCTCTGAGTCTGAGTCGCTCTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGACTCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtct                                                                                           <  2:1004162/141‑1 (MQ=11)

                                                  tctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtct                                                                                           >  1:1094188/1‑141 (MQ=255)

                                                   ctgaTTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctatctg                                                                                          <  2:731895/141‑1 (MQ=11)

                                                       aTCGCTGTCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCtgagtcgctgtctgaatctgagtcgctgtctgaatc                                                                                      <  2:535785/141‑1 (MQ=255)

                                                       aTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCtgagtcgctgtctgaatctgagtcgctgtctgaatc                                                                                      >  2:279539/1‑141 (MQ=255)

                                                       aTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCtgagtcgctgtctgaatctgagtcgctgtctgaatc                                                                                      >  1:942217/1‑141 (MQ=255)

                                                       aTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCtgagtcgctgtctgaatctgagtcgctgtctgaatc                                                                                      >  1:854619/1‑141 (MQ=255)

                                                         cGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGGTGTCTGAGTCTGcgtcggtgtgggaatctgagtcgctctctgagtctg                                                                                    >  2:823320/1‑141 (MQ=11)

                                                          gCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATATGAATCTGAGTCGATGTCTGAGTCTGAgtcgctgtctgaatctgagtcgctgtctgaatctga                                                                                   <  1:175012/141‑1 (MQ=255)

                                                           cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGtctgaatctgaa                                                                                  <  2:268641/141‑1 (MQ=255)

                                                              tctgaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtctttcatatttata                                                                               >  1:988593/1‑129 (MQ=255)

                                                                     ctgaGTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctg                                                                        <  2:776786/141‑1 (MQ=255)

                                                                       gaGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTC‑‑AG‑‑‑‑TGTCTGAGTCTGAATCGCTGTCTGTGTCTGAGTCGGTGTCCGAGTCTGAGTCGCTGTCTGAGTCTGAGTCAGTGTCTGAATCTGAATCGGTAtctgaatctgaa                                                                >  2:797750/1‑141 (MQ=0)

                                                                        aGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGTATCTGAATCGCTgtctgtgt                                                                     >  2:457958/1‑141 (MQ=255)

                                                                         gTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgagtc                                                                    >  1:656848/1‑141 (MQ=255)

                                                                            gCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgagtctga                                                                 >  1:190229/1‑141 (MQ=255)

                                                                             gcaatgcagtctgagtcggtgtatgagtctgcgtcgctgtctgAGACTGATTCGATATCTATATCTGCGCCGCCCCCTGATTCTTAGTCTTTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaa                                                                <  1:713474/134‑1 (MQ=9)

                                                                             cTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaa                                                                <  1:404973/141‑1 (MQ=255)

                                                                             cTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaa                                                                <  2:224186/141‑1 (MQ=255)

                                                                               aTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatc                                                              <  1:141314/141‑1 (MQ=255)

                                                                                tctgagtctgagtcgctgtctgagtctgagtcgctgTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcg                                                             >  1:572541/1‑141 (MQ=255)

                                                                                 cggagtctgggtcgctgtctgagtctgagtcgctgtctGAGTCTGAATCGCTGTCTGAATCCGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgc                                                            <  2:1136255/139‑1 (MQ=14)

                                                                                  tgagtctgagtcgctgtctgagtctgagtcgctgtcTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGATTCTGAGTCGCTGtctgaatctgaatcgct                                                           <  1:566550/141‑1 (MQ=14)

                                                                                   gagtctgagtcgctggctgagtctgagtcgctgtctGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgcta                                                          <  1:990850/141‑1 (MQ=255)

                                                                                       ctgagtcgctgtctgagtctgagtcgctgtctgagtCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctg                                                      <  2:969788/141‑1 (MQ=255)

                                                                                        tgagtcgctgtctgagtctgagtcgctgtctgagtcTGAATCGCTATCTGACTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctga                                                     <  2:669823/141‑1 (MQ=255)

                                                                                        tgagtcgcgctctgagtctgagtcgctgtctgagtcTGAATCGCTATCTGACTCTGAGTCGCTGTCTGACTCTGAGTCGCCGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgattctgaatcgctatctga                                                     <  2:708279/141‑1 (MQ=17)

                                                                                         gagtcgctgtctgagtctgagtcgctgtctgagtctGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgaatctgaatcgctatctgag                                                    <  1:624649/141‑1 (MQ=255)

                                                                                           gtcgctgtctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTg                                                                            >  2:959179/1‑115 (MQ=255)

                                                                                           gtcgctgtctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTg                                                                            <  1:959179/115‑1 (MQ=255)

                                                                                           gtcgctgtctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtc                                                  >  2:563122/1‑141 (MQ=255)

                                                                                           gtcgctgtctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgaatctgaatcgctatctgagtc                                                  >  2:382977/1‑141 (MQ=255)

                                                                                                 gtctgagtctgagtcgctgtctgcgtccgaGTCGCTAACCGAATACGAGACGCCATACGTGTCCGCGTCCCAGCCGGAACCGCAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatc                                            <  1:113361/141‑1 (MQ=9)

                                                                                                   ctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatcgc                                          >  1:561010/1‑141 (MQ=255)

                                                                                                    tgagtctgagtcgctgtctgagtctgaCTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGtctgagtctgaatcgctatctgagtctgaatcgct                                         <  2:267518/141‑1 (MQ=255)

                                                                                                    tgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatcgct                                         >  1:52633/1‑141 (MQ=255)

                                                                                                     gagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatcgcta                                        >  1:615732/1‑141 (MQ=255)

                                                                                                       gtctgagtcgctgtctgagtctgaCTCGCTATCTGCATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCtgagtctgaatcgctatctgagtctgaatcgctatc                                      <  2:1044292/141‑1 (MQ=255)

                                                                                                        tctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                                                                                                      <  1:235954/60‑1 (MQ=38)

                                                                                                        tctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                                                                                                      >  2:235954/1‑60 (MQ=38)

                                                                                                         ctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGagtctgaatcgctatctgagtctgaatcgctatctg                                    >  1:404589/1‑141 (MQ=255)

                                                                                                         ctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGagtctgaatcgctatctgaatctgaatcgctatctg                                    >  2:337995/1‑141 (MQ=255)

                                                                                                          tgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAgtctgaatcgctatctgagtctgaatcgctatctga                                   <  2:433983/141‑1 (MQ=255)

                                                                                                           gagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAgtcagaatcgctatctgaatctgaatcgctatctgaa                                  >  2:45620/1‑140 (MQ=255)

                                                                                                           gagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGtctgaatcgctatctgagtctgaatcgctatctgag                                  <  1:416569/141‑1 (MQ=255)

                                                                                                             gtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCtgaatcgctatctgagtctgaatcgctatctgagtc                                <  1:695197/141‑1 (MQ=255)

                                                                                                                gctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAatcgctatctgagtctgaatcgctatctgagtctga                             >  1:681108/1‑141 (MQ=255)

                                                                                                                gctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAACCTGAGTCGCTGTCTGAATGTGAATCGCTGtctgagtctgaagcgctatctgaatctgagtcggtgggggagtccga                             >  2:162405/1‑127 (MQ=14)

                                                                                                                 ctgtctgagtctgaATCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGATTCTGACTCGCTGTCTGAGTCTGAAtcgctatctgagtctgaatcgctatctgagtctgaa                            <  2:1028479/141‑1 (MQ=255)

                                                                                                                 ctgtctgagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAAtcgctatctgagtctgaatcgctatctgagtctgaa                            <  2:572541/141‑1 (MQ=255)

                                                                                                                 ctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAAtcgctatctgagtctgaatcgctatctgagtctgaa                            <  2:1117531/141‑1 (MQ=255)

                                                                                                                 ctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAAtcgctatctgagtctgaatcgctatctgagtctgaa                            <  1:77853/141‑1 (MQ=255)

                                                                                                                   gtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGACTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAGTCgctatctgagtctgaatcgctatctgagtctgaatc                          <  2:564326/141‑1 (MQ=255)

                                                                                                                   gtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCgctatctgagtctgaatcgctatctgagtctgaatc                          <  1:895525/141‑1 (MQ=255)

                                                                                                                     ctgagtctgaATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCAc                        <  2:707975/141‑1 (MQ=255)

                                                                                                                       gagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTg                      >  1:1106425/1‑141 (MQ=255)

                                                                                                                         gtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGATTCTGAGTCGCTATCTGCGTCTGATTCGCAATGAGTGTCTGCATCACTGTc                    >  2:870755/1‑141 (MQ=14)

                                                                                                                              aaTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGCGTCTGAATCGCTATCTGAGCTTGAATCGCTATATGAGTCAGAATCTCTGTCTGAGt               >  2:289642/1‑141 (MQ=17)

                                                                                                                               aTCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctga                                                                                                     <  2:122267/54‑1 (MQ=255)

                                                                                                                               aTCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctga                                                                                                     >  1:122267/1‑54 (MQ=255)

                                                                                                                               aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTc              >  2:1190057/1‑141 (MQ=255)

                                                                                                                               aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTc              >  1:739520/1‑141 (MQ=255)

                                                                                                                                tCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaa                                                                                                          >  1:456991/1‑48 (MQ=255)

                                                                                                                                tCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGGCGCTGTCTGAGTCTGAGTCACTGTCTGAGccc             >  2:27696/1‑141 (MQ=14)

                                                                                                                                 cGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGATTCTGAATCGCTGTCNGAGTCCg            >  2:897876/1‑141 (MQ=255)

                                                                                                                                 cGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCg            >  1:1093124/1‑141 (MQ=255)

                                                                                                                                       ctgactctgaGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCAc      <  2:1066171/141‑1 (MQ=255)

                                                                                                                                       ctgaatctgaGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCAc      >  2:197301/1‑141 (MQ=255)

                                                                                                                                           atctggGTCGCTGTCTGATTCTGAGTCGCTGTCTGACTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCACTGTc  <  2:948277/141‑1 (MQ=255)

                                                                                                                                           atctgaGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCACTGTc  <  2:1052656/141‑1 (MQ=255)

                                                                                                                                           atctgaGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCACTGTc  <  1:63924/141‑1 (MQ=255)

AATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCACTGTC  >  CP000730/2774914‑2775193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`