Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,846,695	A→G	5.2%	V34V (GTT→GTC)	cbiQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,846,695	0	A	G	5.2%	244.9 / 3.0	77	V34V (GTT→GTC)	cbiQ	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (50/23);  new base G (2/2);  total (52/25)
Fisher's exact test for biased strand distribution p-value = 5.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

ATGCTCCGCCTATCACTACCTACACCCGGGTCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTAAAACTTCAAAAAAATATTA  >  CP000730/2846555‑2846834                                                                                                                                             |                                                                                                                                            aggatCCGCCTATCACTCCCTACACCGGGGGCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCa                                                                                                                                             >  2:608703/5‑141 (MQ=255) aTGCTCCGCCTATCACTACCTACACCCGGGTCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCa                                                                                                                                             >  1:514554/1‑141 (MQ=255) aTGCTCCGCCTATCACTACCTACACCCGGGTCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCa                                                                                                                                             >  2:463114/1‑141 (MQ=255)   gCTCCGCCTATCACTACCTACACCCGGGTCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAac                                                                                                                                           >  1:1076379/1‑141 (MQ=255)        gCCTATCACTACCTACACCCGGGTCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGta                                                                                                                                      <  1:872235/141‑1 (MQ=255)         ccTATCACTACCTACACCCGGGTCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGtat                                                                                                                                     >  1:346908/1‑141 (MQ=255)            aTCACTACCTACACCCGGGTCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATatc                                                                                                                                  >  2:216930/1‑141 (MQ=255)             tCACTACCTACACCCGGGTCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATatca                                                                                                                                 <  2:641054/141‑1 (MQ=255)              cACTACCTACACCCGGGTCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATatcat                                                                                                                                >  2:445705/1‑141 (MQ=255)               aCTACCTACACCCGGGTCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATatcatc                                                                                                                               >  2:481195/1‑141 (MQ=255)                 tacctacACCCGGGTCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCAc                                                                                                                             >  1:536535/1‑141 (MQ=255)                   cctacACCCGGGTCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCGACACGTATATCATCACtt                                                                                                                           <  1:480199/141‑1 (MQ=255)                    ctacTCCCGGGTCTACGTCTGATACAAAATCTGTGATTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTCAAGTTTTCAACACGTATCTCATCGCTTa                                                                                                                          <  2:372977/141‑1 (MQ=255)                      acacCCGGGTCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACa                                                                                                                        >  2:495528/1‑141 (MQ=255)                       cacCCGGGTCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAg                                                                                                                       <  1:563795/141‑1 (MQ=255)                            gggTCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTatat                                                                                                                  >  2:687380/1‑141 (MQ=255)                                   ccACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTc                                                                                                           >  1:37579/1‑141 (MQ=255)                                   ccACTGAGACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACTCAAATGTCATACGGTGGTATATCATGCGTTATGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTc                                                                                                           >  1:286267/1‑141 (MQ=255)                                            cAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTg                                                                                                  >  1:341781/1‑141 (MQ=255)                                              aaaaCTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCa                                                                                                >  1:316635/1‑141 (MQ=255)                                              aaaaCTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGAGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCa                                                                                                >  1:1162109/1‑141 (MQ=255)                                               aaaCTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCAc                                                                                               >  1:187972/1‑141 (MQ=255)                                                aaCTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCGACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACt                                                                                              <  1:271201/141‑1 (MQ=255)                                                  ctggcctaaaggcacAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTa                                                                                            >  1:242993/15‑141 (MQ=255)                                                  cTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTa                                                                                            >  2:216524/1‑141 (MQ=255)                                                  cTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTa                                                                                            <  2:1111124/141‑1 (MQ=255)                                                      gCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAgc                                                                                        >  2:1099277/1‑141 (MQ=255)                                                      gCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAgc                                                                                        >  2:1085868/1‑141 (MQ=255)                                                      gCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAgc                                                                                        >  2:1085827/1‑141 (MQ=255)                                                      gCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTGCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAgc                                                                                        >  2:716133/1‑141 (MQ=255)                                                                cAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTa                                                                              >  1:841682/1‑141 (MQ=255)                                                                 aGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTaa                                                                             >  1:190551/1‑141 (MQ=255)                                                                   tATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGt                                                                           <  1:807140/141‑1 (MQ=255)                                                                    aTTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTc                                                                          <  1:89479/141‑1 (MQ=255)                                                                     ttttACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTcc                                                                         <  2:38255/141‑1 (MQ=255)                                                                     ttttACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTcc                                                                         <  2:268872/141‑1 (MQ=255)                                                                            gTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCt                                                                  >  2:190591/1‑141 (MQ=255)                                                                               tGATATAAACGATATGACGCTACCCAAATGTCATACGGCGGTCTATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGAt                                                               >  1:1023730/1‑141 (MQ=255)                                                                                  tataAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGc                                                            <  1:1153838/141‑1 (MQ=255)                                                                                  tataAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGc                                                            >  1:1122469/1‑141 (MQ=255)                                                                                   ataAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAAGCTGATTGCa                                                           <  1:1048583/141‑1 (MQ=255)                                                                                    taAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCaa                                                          >  2:1104823/1‑141 (MQ=255)                                                                                      aaCGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATa                                                        >  2:81599/1‑141 (MQ=255)                                                                                      aaCGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATa                                                        >  2:248093/1‑141 (MQ=255)                                                                                      aaCGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATa                                                        >  2:879981/1‑141 (MQ=255)                                                                                      aaCGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATa                                                        >  1:564917/1‑141 (MQ=255)                                                                                         gATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCGACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAAAACCa                                                     >  2:147812/1‑141 (MQ=255)                                                                                         gATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCa                                                                                                         >  1:983927/1‑89 (MQ=255)                                                                                         gATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCa                                                                                                         <  2:983927/89‑1 (MQ=255)                                                                                              gACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAaataa                                                >  1:195063/1‑141 (MQ=255)                                                                                               aCGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAaataat                                               >  1:731568/1‑141 (MQ=255)                                                                                               aCGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAaaaaat                                               >  2:832850/1‑141 (MQ=255)                                                                                               aCGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCAATTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAaataat                                               >  1:169850/1‑141 (MQ=255)                                                                                                     cccAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCa                                         >  2:159190/1‑141 (MQ=255)                                                                                                       cAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATa                                       >  1:721112/1‑141 (MQ=255)                                                                                                             tCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGc                                 <  2:403367/141‑1 (MQ=255)                                                                                                              cATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGcc                                <  1:523455/141‑1 (MQ=255)                                                                                                               aTACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGcct                               >  2:569498/1‑141 (MQ=255)                                                                                                               aTACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGcct                               >  1:677009/1‑141 (MQ=255)                                                                                                                 aCGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGcctcc                             <  2:1181340/141‑1 (MQ=255)                                                                                                                   ggtggtATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCtt                           <  1:342597/141‑1 (MQ=255)                                                                                                                      ggtATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCttttt                        >  2:430449/1‑141 (MQ=255)                                                                                                                       gtATATCATGCGTTAAGTTTTCGACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAAAACCAAATAATTTCCCATAAAATGCCTCCtttttt                       >  1:319424/1‑141 (MQ=255)                                                                                                                           atCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTaaa                   <  2:904071/141‑1 (MQ=255)                                                                                                                             cATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTAAAAc                 >  1:666908/1‑141 (MQ=255)                                                                                                                              aTGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTAAAACt                <  1:630841/141‑1 (MQ=255)                                                                                                                              aTGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTACATAAATTAAAAAAAAAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTaaaaaa                <  2:964935/141‑3 (MQ=255)                                                                                                                              aTGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTAAAAAAATAGAAATAACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTaaaaaaaa                <  2:214585/141‑3 (MQ=255)                                                                                                                              aTGAGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTAAAACt                >  2:1186324/1‑141 (MQ=255)                                                                                                                                gCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTAAAACTTc              >  2:1015544/1‑141 (MQ=255)                                                                                                                                 cGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTAAAACTTCa             >  1:339080/1‑141 (MQ=255)                                                                                                                                 cGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTAAAACTTCa             >  1:338885/1‑141 (MQ=255)                                                                                                                                  gTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTAAAACTTCaa            <  1:580333/141‑1 (MQ=255)                                                                                                                                   ttAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTAAAACTTCaaa           >  1:136724/1‑141 (MQ=255)                                                                                                                                    tAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTAAAACTTCaaaa          <  2:356917/141‑1 (MQ=255)                                                                                                                                       gTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTAAAACTTCaaaaaaa       <  1:1043036/141‑1 (MQ=255)                                                                                                                                          ttCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTAAAACTTCAAAAAAatat    >  2:889280/1‑141 (MQ=255)                                                                                                                                           tCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTAAAACTTCAAAAAAAtatt   >  2:580516/1‑141 (MQ=255)                                                                                                                                           tCAACAAGTATATACTCACTTACAGTATATGCAACTCCATACATTGCACATACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTAAAACTTCAAAAAAAtatt   >  2:108725/1‑141 (MQ=255)                                                                                                                                            cAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTAAAACTTCAAAAAAAtatta  >  1:627052/1‑141 (MQ=255)                                                                                                                                             |                                                                                                                                            ATGCTCCGCCTATCACTACCTACACCCGGGTCTACCACTGATACAAAAACTGTGCCTTTAGGCCAGTATTTTACAGTTTGATATAAACGATATGACGCTACCCAAATGTCATACGGTGGTATATCATGCGTTAAGTTTTCAACACGTATATCATCACTTACAGTATATGCAACTCCATACATTGCACTTACAGCGCCATCACTAAGTCCAAAATCTGATTGCAATACCAAATAATTTCCCATAAAATGCCTCCTTTTTTTAAAACTTCAAAAAAATATTA  >  CP000730/2846555‑2846834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑