Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 509,502 T→C 10.5% I85T (ATT→ACT)  dnaZX → DNA‑directed DNA polymerase III gamma and tau subunits

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730509,5020TC10.5% 97.0 / 3.5 38I85T (ATT→ACT) dnaZXDNA‑directed DNA polymerase III gamma and tau subunits
Reads supporting (aligned to +/- strand):  ref base T (24/10);  new base C (2/2);  total (26/12)
Fisher's exact test for biased strand distribution p-value = 5.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

GAGGTACGGGGAAAACGAGTATTGCCAAAGTGTTTGCTAAAGCAATCAACTGTCTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTAACAACAGGTGCTTTTAATGCCCTTT  >  CP000730/509364‑509642
                                                                                                                                          |                                                                                                                                            
gagGTACGGGGAAAACGAGTATTGCCAAAGTGTTTGCTAAAGCAATCAACTGTCTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTg                                                                                                                                            <  2:156511/141‑1 (MQ=255)
    tACGGGGAAAACGAGTATTGCCAAAGTGTTTGCTAAAGCAGTCAACTGTCTAAATAGCACTGATGGAGAAACTTGTAATGAATGTCATATTTGTAAAGGAATTACGTAGGGGACTAATTCAGATGTGATAGAAATTGTtgc                                                                                                                                        >  2:352897/1‑141 (MQ=255)
        gggTAAACGAGTATTGCCAAAGTGTTTGCTAAAGCAATCAACTGTCTAAATAGCACTGATGGAGAACCTTGTGATGAATGTCATATTTGTCAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATgctgct                                                                                                                                    >  2:431482/1‑141 (MQ=255)
         ggAAAACGAGTATTGCCAAAGTGTTTGCTAAAGCAATCAACTGTCTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTa                                                                                                                                   <  2:131737/141‑1 (MQ=255)
              aCGAGTATTGCCAAAGTGTTTGCTAAAGCAATCAACTGTCTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGtaat                                                                                                                              <  1:151041/141‑1 (MQ=255)
                       gCCAAAGTGTTTGCTAAAGCAATCAACTGTCTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCAGTACGCAGGGGACTAATTCAGATGTGGTAGAAATTGATGCTGCTAGTAAGAATGGGGtt                                                                                                                     >  2:404144/1‑141 (MQ=255)
                        ccAAAGTGTTTGCTAAAGCAATCAACTGTCTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTtg                                                                                                                    >  2:461609/1‑141 (MQ=255)
                         cAAAGTGTTTGCTAAAGCAATCAACTGTCTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTtga                                                                                                                   >  2:5959/1‑141 (MQ=255)
                            aGTGTTTGCTAAAGCAATCAACTGTCTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTtgatga                                                                                                                >  1:178866/1‑141 (MQ=255)
                                  tGCTAAAGCAATCAACTGTCTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAg                                                                                                          <  1:290923/141‑1 (MQ=255)
                                   gCTAAAGCAATCAACTGTCTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGa                                                                                                         <  1:5959/141‑1 (MQ=255)
                                              cAACTGTCTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAgaga                                                                                              >  2:59389/1‑141 (MQ=255)
                                              cAACTGTCTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTAGATGAAATAAGAAATATTAggga                                                                                              >  1:190895/1‑141 (MQ=255)
                                                  tGTCTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACaaa                                                                                          >  1:268451/1‑141 (MQ=255)
                                                     cTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAATGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGTTGCTAGTAATAATGGGGTTGATGAAATAAGTAATATTagaggctatgtt                                                                                       <  2:436100/141‑9 (MQ=255)
                                                         aTAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAat                                                                                   >  2:408433/1‑141 (MQ=255)
                                                         aTAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAat                                                                                   >  2:418831/1‑141 (MQ=255)
                                                                 gATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCa                                                                           >  2:326858/1‑141 (MQ=255)
                                                                         aCCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAACTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATc                                                                   >  2:141677/1‑141 (MQ=255)
                                                                         aCCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAACTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATc                                                                   >  2:141574/1‑141 (MQ=255)
                                                                            ttGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGaa                                                                >  2:303034/1‑141 (MQ=255)
                                                                                      tgTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATAGATACTGCTAGTAATTATGGGGTTGATGAAATAATAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGtt                                                      <  1:351011/141‑1 (MQ=255)
                                                                                        tCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTtta                                                    >  1:19503/1‑141 (MQ=255)
                                                                                         cATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTttat                                                   >  1:219367/1‑141 (MQ=255)
                                                                                                gTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAg                                            >  2:387904/1‑141 (MQ=255)
                                                                                                         ttACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGc                                   >  2:244504/1‑141 (MQ=255)
                                                                                                              cAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATg                              >  2:330488/1‑141 (MQ=255)
                                                                                                                 gggACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTa                           >  2:56821/1‑141 (MQ=255)
                                                                                                                      tAATTCAGATGTGATAGAAACTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTaacaac                      <  1:141574/141‑1 (MQ=255)
                                                                                                                      tAATTCAGATGTGATAGAAACTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTaacaac                      <  1:141677/141‑1 (MQ=255)
                                                                                                                         ttCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTAACAACAgg                   <  2:324557/141‑1 (MQ=255)
                                                                                                                         ttCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTAACAACAgg                   >  1:479476/1‑141 (MQ=255)
                                                                                                                         ttCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTAACAACAgg                   >  2:469894/1‑141 (MQ=255)
                                                                                                                          tCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTAACAACAGGt                  <  2:68914/141‑1 (MQ=255)
                                                                                                                                gtgATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTAACAACAGGTGCtttt            <  1:28431/141‑1 (MQ=255)
                                                                                                                                 tgggaGAAATTGATGCTGCGGGTAATAATGGGGTGGATGAAATAAGAAAGATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAAGTGCACATGCTAACAACAGATGCTTTTa           >  2:427215/5‑141 (MQ=255)
                                                                                                                                         aTTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTAACAACAGGTGCTTTTAATGCCCtt   >  1:173304/1‑141 (MQ=255)
                                                                                                                                         aTTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTAACAACAGGTGCTTTTAATGCCCtt   >  1:173303/1‑141 (MQ=255)
                                                                                                                                          ttGATGCTTCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGATAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTTACAACAGGTGCTTTTAATGCCCttt  <  2:275125/141‑1 (MQ=255)
                                                                                                                                          ttGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTAACAACAGGTGCTTTTAATGCCCttt  <  1:352897/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
GAGGTACGGGGAAAACGAGTATTGCCAAAGTGTTTGCTAAAGCAATCAACTGTCTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTAACAACAGGTGCTTTTAATGCCCTTT  >  CP000730/509364‑509642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑