Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 849,656 G→A 56.8% G261D (GGT→GAT)  yvcJ → ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730849,6560GA56.8% 17.5 / 47.4 44G261D (GGT→GAT) yvcJATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (10/9);  new base A (4/21);  total (14/30)
Fisher's exact test for biased strand distribution p-value = 2.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATTAGATAAAGACGTTTATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCGAGAAAAAATGAGACAAA  >  CP000730/849517‑849792
                                                                                                                                           |                                                                                                                                        
ggAGTAGATAAAGACGCTTATAATTATGTTAGGAAAGGTAAAGAGACGGAGATTTTCTTTGAAAAATTAATTAAGTTGTTAGATTTTATGATACCCGGGTATCAAAAAGAAGGGACATCTCAATTAGTAATTGCCATCGGt                                                                                                                                         <  2:430124/141‑1 (MQ=255)
    tAGATAAAGACGTTTATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTa                                                                                                                                     <  2:53707/141‑1 (MQ=255)
      aaTAAAGACGTTTATAATTATGTTATGAAATGGAAAGAGACGGAGATTTACTTTAAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACg                                                                                                                                   <  2:231817/140‑1 (MQ=255)
        tAAAGACGTTTATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAAGTGCCATCGGTTGTACggg                                                                                                                                 <  2:170671/141‑1 (MQ=255)
          aaaaCGTGTATGATTGAGTTATGAGATGGAAAGAGACGGAGATTTTCTTTGAAAAAGTGACTGATTTGTTAGATTTTATGATACCCGATTATAAAAAAGAAGGGTAATCTCAATTAGTAATTGCCTTCGATTGTACGGGTg                                                                                                                               <  2:369640/138‑1 (MQ=255)
          aaGACGTTTATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTg                                                                                                                               <  2:338617/141‑1 (MQ=255)
          aaGACGTTTATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTGAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTg                                                                                                                               <  2:158013/141‑1 (MQ=255)
                        tatgttatgAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCt                                                                                                                 <  1:275076/141‑1 (MQ=255)
                                     ggAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAg                                                                                                    <  1:454119/141‑1 (MQ=255)
                                         agagACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACg                                                                                                >  2:350311/1‑141 (MQ=255)
                                         agagACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACg                                                                                                <  1:123534/141‑1 (MQ=255)
                                         agagACGGAGATTTTATTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACg                                                                                                <  2:146973/141‑1 (MQ=255)
                                            gaCGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACt                                                                                             <  1:324358/141‑1 (MQ=255)
                                              cGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAg                                                                                           <  1:118120/141‑1 (MQ=255)
                                                   aTTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAAt                                                                                      <  2:176959/141‑1 (MQ=255)
                                                      ttCTTTGAAAAATTAACTGATTTGTTAGATTTTAGGATATCCGGGTATAAACAAGAAGGGAAATCTCAATGAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTACTTAt                                                                                   <  2:472718/141‑1 (MQ=255)
                                                             aaaaaTTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGGGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATg                                                                            >  1:272571/1‑141 (MQ=255)
                                                                       tGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGa                                                                  <  2:476003/141‑1 (MQ=255)
                                                                       tGATTTGTTAGATTTTATGATACAAGAAAATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGa                                                                  <  2:476010/141‑1 (MQ=255)
                                                                         aTTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAat                                                                <  1:135676/141‑1 (MQ=255)
                                                                             gTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATaa                                                            >  2:18595/1‑141 (MQ=255)
                                                                                    tttATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGac                                                                                                                            <  2:492049/70‑1 (MQ=255)
                                                                                    tttATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGac                                                                                                                            >  1:492049/1‑70 (MQ=255)
                                                                                       aTGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTAtgtg                                                  <  1:393811/141‑1 (MQ=255)
                                                                                          aTACCCGGGTATAACAAAGAAGGAAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTCTATAACTGAAGTATTCGAATATAATGTTTATGTGcat                                               >  2:136693/1‑141 (MQ=255)
                                                                                                 ggTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAggg                                        <  1:334309/141‑1 (MQ=255)
                                                                                                   tataAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCCTAGGGAc                                      <  1:445683/141‑1 (MQ=255)
                                                                                                     taAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGc                                    >  2:353522/1‑141 (MQ=255)
                                                                                                     taAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGcgtccctatgatgc                                    <  2:155587/141‑14 (MQ=255)
                                                                                                     taAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGcgtccctatgatgc                                    <  2:155584/141‑11 (MQ=255)
                                                                                                       aaaaaGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGcac                                  >  2:89457/1‑141 (MQ=255)
                                                                                                              agGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGa                           <  2:206953/141‑1 (MQ=255)
                                                                                                               gggAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGaa                          >  2:454279/1‑141 (MQ=255)
                                                                                                                        cAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCgag                 <  1:181786/141‑1 (MQ=255)
                                                                                                                        cAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCgag                 <  1:171837/141‑1 (MQ=255)
                                                                                                                          aTTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCGAGaa               <  1:185842/141‑1 (MQ=255)
                                                                                                                          aTTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCGAGaa               >  2:164208/1‑141 (MQ=255)
                                                                                                                            tAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAg                                                                                           <  2:12456/63‑1 (MQ=255)
                                                                                                                            tAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAg                                                                                           >  1:12456/1‑63 (MQ=255)
                                                                                                                              gTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATATAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACTCACATATTGAAAGTGGCGAGaaaaaa           <  2:226139/141‑1 (MQ=255)
                                                                                                                                aaTTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCGAGAAAAAATg         >  2:364406/1‑141 (MQ=255)
                                                                                                                                 aTTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCGAGAAAAAATga        >  2:313357/1‑141 (MQ=255)
                                                                                                                                   tGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCGAGAAAAAATgaga      >  1:55418/1‑141 (MQ=255)
                                                                                                                                       aTCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCGAGAAAAAATGAGACaaa  >  1:236780/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                        
GGATTAGATAAAGACGTTTATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCGAGAAAAAATGAGACAAA  >  CP000730/849517‑849792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑