| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,200,415 | C→T | 18.0% | A77V (GCT→GTT) | ylmH → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,200,415 | 0 | C | T | 18.0% | 47.3 / 2.7 | 22 | A77V (GCT→GTT) | ylmH | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (5/13); new base T (2/2); total (7/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
AACTAAGCACTGAAATTGAACGTCTTAAAGAAGATAAAAAACAACTTGAAAAAGTAATCGAAGAGAGAGATACTAATATTAAGTCTTATCAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGCTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCTAATCAAATGGTTGGTGACGCGGTAGAAAAAGCACGCCGTTTAGCATTCCAGACTGAAGATATGAAACGT > CP000730/1200277‑1200554 | aaCTAAGCACTGAAATTGAACGTCTTAAAGAAGATAAAAAACAACTTGAAAAAGTAATCGAAGAGAGAGATACTAGTATTAAGTCTTATCAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGCTg < 1:13317/141‑1 (MQ=255) aaGCACTGAAATTGAACGTCTTAAAGAAGATAAAAAACAACTTGAAAAAGTAATCGAAGAGAGAGATACTAATATTAAGTCTTATCAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGTTGGTga > 2:101982/1‑141 (MQ=255) aaGCACTGAAATTGAACGTCTTAAAGAAGATAAAAAACAACTTGAAAAAGTAATCGAAGAGAGAGATACTAATATTAAGTCTTATCAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGCTGGTga < 2:364720/141‑1 (MQ=255) aaGCACTGAAATTGAACGTCTGAAAGAAGATAAAAAACAACTTGAAAAAGTAATCGAAGAGAGAGATACTAATATTAAGTCTTATCAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGTTGGTga > 2:102005/1‑141 (MQ=255) aagaTAAAAAACAACTTGAAAAAGTAATCGAAGAGAGAGATACTAATATTAAGTCTTATCAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGCTGGTGAAGAAACTAAGCAAGCTGCAGAGAAAc < 2:47208/141‑1 (MQ=255) aTAAAAAACAACTTGAAAAAGTAATCGAAGAGAGAGATACTAATATTAAGTCTTATCAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGCTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAg > 1:477472/1‑141 (MQ=255) aaaaaaCAACTTAAAAAAGTAATCGAAGAGAGAGATACTAATATTAAATCTTATCAAGACGTGAATCAATCTGTAAGTGATGCTTAAAAAAAAAAAAAAAAAGCTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAGCt < 2:164886/141‑1 (MQ=255) ttGAAAAAGTAATCGAAGAGAGAGATACTAATATTAAGTCTTATCAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGCTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATta < 1:109340/141‑1 (MQ=255) tGAAAAAGTAATCGAAGAGAGAGATACTAATATTAAGTCTTATCAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGCTTGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATtat < 1:417265/141‑1 (MQ=255) tCTTATCAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGCTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCTAATCAAAtggttggt < 2:490850/141‑1 (MQ=255) cTTATCAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGCTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCTAATCAAATGGTTGGTg < 1:469915/141‑1 (MQ=255) tCAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGCTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCTAATCAAATGGTTGGTGAcgc < 2:421797/141‑1 (MQ=255) cAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGTTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCTAATCAAATGGTTGGTGAcgcg < 1:101982/141‑1 (MQ=255) cAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGTTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCTAATCAAATGGTTGGTGAcgcg < 1:102005/141‑1 (MQ=255) tGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGCTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCTAATCAAATGGTTGGTGACGCGGTAGaaa < 1:482382/141‑1 (MQ=255) tAAGTGATGCTTTGATACAAGCTCAAAAAGCTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCTAATCAAATGGTTGGTGACGCGGTAGAAAAAGCACGCCGtt > 2:21194/1‑141 (MQ=255) gTGATGCTCTGATACAAGGCCAAAAAGCTGGTGAAAAAACTCAGCAAGATGCAGAGAAAAAAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCTAATCAAATGATTGGTGACGCGGTAGAAAATGCACACCGTTTAg < 2:116968/141‑1 (MQ=255) gCTTTGATACAAGCTCAAAAAGCTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACGAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCTAATCAAATGNTTGGTGACGCGGTAGAAAAAGCACGCCGTTTAGCATTc < 1:211563/141‑1 (MQ=255) aTACAAGCTCAAAAAGCTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCTAATCAAATGGTTGGTGACGCGGTAGAAAAAGCACGCCGTTTAGCATTCCAGACt > 2:316649/1‑141 (MQ=255) aTACAAGCTCAAAAAGCTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCTAATCAAATGGTTGGTGACGCGGTAGAAAAAGCACGCCGTTTAGCATTCCAGACt > 2:63984/1‑141 (MQ=255) aaGCTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCTAATCAAATGGTTGGTGACGCGGTAGAAAAAGCACGCCGTTTAGCATTCCAGACTGAAGATATGAAAc < 2:303247/141‑1 (MQ=255) gCTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCTAATCAAATGGTTGGTGACGCGGTAGAAAAAGCACGCCGTTTAGCATTCCAGACTGAAGATATGAAACGt > 2:272036/1‑141 (MQ=255) | AACTAAGCACTGAAATTGAACGTCTTAAAGAAGATAAAAAACAACTTGAAAAAGTAATCGAAGAGAGAGATACTAATATTAAGTCTTATCAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGCTGGTGAAGAAACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCTAATCAAATGGTTGGTGACGCGGTAGAAAAAGCACGCCGTTTAGCATTCCAGACTGAAGATATGAAACGT > CP000730/1200277‑1200554 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |