Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,231,411 A→G 100% D19G (GAT→GGT)  prpC → possible PP2C protein phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,231,4110AG100.0% 172.8 / NA 50D19G (GAT→GGT) prpCpossible PP2C protein phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/20);  total (30/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCGTGAACAAGGTTCGGATATTGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAA  >  CP000730/1231274‑1231545
                                                                                                                                         |                                                                                                                                      
tcgtGAACAAGGTTCGGATATTGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGc                                                                                                                                     >  1:122356/1‑141 (MQ=255)
tcgtGAACAAGGTTCGGATATTGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGc                                                                                                                                     >  1:179894/1‑141 (MQ=255)
 cgtGAACAAGGTTCGGATATTGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCg                                                                                                                                    >  1:250099/1‑141 (MQ=255)
   tGAACAAGGTTCGGATATTGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCggg                                                                                                                                  <  1:111927/141‑1 (MQ=255)
   tGAACAAGGTTCGGATATTGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCggg                                                                                                                                  <  1:208235/141‑1 (MQ=255)
     agcgaGGTTCGGATATTGCCGCAGGTTGTGGTCAATTAAGAGGAAGGGAACGACAAGTAGAAACGAGGTAAGGACAAATGCTAGAGGCGCCATTTTTTGCTGATACTGGACAACATAGAGATACGAATGAAGGTGCGggtg                                                                                                                                <  2:11199/137‑1 (MQ=255)
     aaCAAGGTTCGGATATTGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGggtg                                                                                                                                >  1:359269/1‑141 (MQ=255)
      aCAAGGTTCGGATATTGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGggtgg                                                                                                                               >  1:288839/1‑141 (MQ=255)
      aCAAGGTTCGGATATTGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGggtgg                                                                                                                               <  2:2618/141‑1 (MQ=255)
         aGGTTCGGATATTGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACTATTTTTTACTGATACTCGAGTACATACGGATAAGAATTAATGTGCGTGTGGTAt                                                                                                                            <  2:425321/141‑1 (MQ=255)
                  tatTGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATaa                                                                                                                   <  2:236673/141‑1 (MQ=255)
                       aCGCAGCTTGTGGTCAATTAAGAGAAAATGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTATAGGCACAATTTTTTACTGTTATTGGACAACATAGAGATAAGAATGAAGGTGCTGGTGGTATTTTTTATAATCaaa                                                                                                              <  2:248517/141‑1 (MQ=255)
                                gtgGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATcaac                                                                                                     <  2:70210/141‑1 (MQ=255)
                                gtgGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATcaac                                                                                                     <  2:70209/141‑1 (MQ=255)
                                gtgGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATcaac                                                                                                     >  1:433852/1‑141 (MQ=255)
                                gtgGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATcaac                                                                                                     <  1:142455/141‑1 (MQ=255)
                                     cAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACtt                                                                                                <  1:192787/141‑1 (MQ=255)
                                      aaTTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACttt                                                                                               <  1:347809/141‑1 (MQ=255)
                                            gagCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTc                                                                                         <  1:218593/141‑1 (MQ=255)
                                             agCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCt                                                                                        >  1:106388/1‑141 (MQ=255)
                                             agCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCt                                                                                        >  1:331325/1‑141 (MQ=255)
                                               cAAAGGAACGACAAGTAGAAACGAGGTAAAGAAAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCtgt                                                                                      >  1:96293/1‑141 (MQ=255)
                                                        gACAAGCAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGAAACGGGACAACATAGAGAAAAGAATGAAGGTGCGGGTGGTATTTTCTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTa                                                                             >  2:470741/1‑141 (MQ=255)
                                                         aCAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTAt                                                                            >  2:304198/1‑141 (MQ=255)
                                                          cAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATg                                                                           <  1:124901/141‑1 (MQ=255)
                                                          cAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATg                                                                           <  1:498565/141‑1 (MQ=255)
                                                          aaaGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATg                                                                           <  2:163312/140‑1 (MQ=255)
                                                              tAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTg                                                                       >  1:338761/1‑141 (MQ=255)
                                                               aGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTgg                                                                      >  1:265099/1‑141 (MQ=255)
                                                               aGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTgg                                                                      <  2:460735/141‑1 (MQ=255)
                                                                 aaaCGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGcc                                                                    >  1:127567/1‑141 (MQ=255)
                                                                   aCGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCAt                                                                  >  1:170624/1‑141 (MQ=255)
                                                                    cGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATa                                                                 >  2:419324/1‑141 (MQ=255)
                                                                              aCAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGgaga                                                       >  1:249006/1‑141 (MQ=255)
                                                                                   tGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTg                                                  >  1:464183/1‑141 (MQ=255)
                                                                                          ggCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTaa                                           >  1:35763/1‑141 (MQ=255)
                                                                                                    tttACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTTATGGTATGGCTGGCCCTACAGCAGGAGCAGTTGCAAGTAAATTTGTTACa                                 >  2:269393/1‑141 (MQ=255)
                                                                                                      tACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGa                               >  1:408129/1‑141 (MQ=255)
                                                                                                      tACTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGa                               >  1:153152/1‑141 (MQ=255)
                                                                                                        tttttttttgACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTTTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATg                             >  2:224787/10‑141 (MQ=255)
                                                                                                        cTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATg                             >  2:227267/1‑141 (MQ=255)
                                                                                                        cTGATACTGGACAACATAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTCCAAGTTATGTGTGACGATATGCGAAGACATAAAGCAGAAGCACTTGCAAGTAAATTTGTTACAGATg                             >  1:298091/1‑141 (MQ=255)
                                                                                                                  acaacaTAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATc                   >  1:68053/1‑141 (MQ=255)
                                                                                                                     acatgtagaTAAGACTGTAGGTGCGGTTGGTATTTTTTATAATCACTGTAGGCGACAACTTTTGGTTCTGTGTGATGGTTTGGGTGGCCATATAGCAGGGCAAGTTGCAAGTAAATCTATTACAGTTGCGTTGAACTGCCg                <  2:120870/135‑1 (MQ=255)
                                                                                                                      caTAGAGATAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCCACAACTTTTAGTTCTCTGTGATGGTATGGGTAGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGt               >  2:494533/1‑141 (MQ=255)
                                                                                                                          gagaTAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTg           >  2:112277/1‑141 (MQ=255)
                                                                                                                          gagaTAAGAATGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTg           >  1:346513/1‑141 (MQ=255)
                                                                                                                                 gAATGAAGGTGCGGGTGGTATTTTTTACAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGt                                                                        >  2:379070/1‑73 (MQ=255)
                                                                                                                                 gAATGAAGGTGCGGGTGGTATTTTTTACAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGt                                                                        <  1:379070/73‑1 (MQ=255)
                                                                                                                                   aTGAAGGTGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGaaa  <  2:338761/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TCGTGAACAAGGTTCGGATATTGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTATTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAA  >  CP000730/1231274‑1231545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑