Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,478,171 T→G 9.1% A7951A (GCA→GCC)  ebh ← extracellular matrix binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,478,1710TG 9.1% 125.1 / 6.8 44A7951A (GCA→GCC) ebhextracellular matrix binding protein
Reads supporting (aligned to +/- strand):  ref base T (20/20);  new base G (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

TTTCATCTGGGTTAAGCGTTGGATTTGTCGTTTGATTGATAATACCTTCCGCTTGAGACACTGCATTTGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTGGTTACGTAATGCATCACGTTGTGGTTGATTTAAA  >  CP000730/1478035‑1478303
                                                                                                                                        |                                                                                                                                    
tttCATCTGGGTTAAGCGTTGGATTTGTCGTTTGATTGATAATACCTTCCGCTTGAGACACTGCATTTGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCaa                                                                                                                                  >  1:279929/1‑141 (MQ=255)
tttCATCTGGGTTAAGCGTTGGATTTGTCGTTTGATTGATAATACCTTCCGCTTGAGACACTGCATTTGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTGGCaa                                                                                                                                  >  1:162985/1‑141 (MQ=255)
tttCATCTGGGTTAAGCGTTGGATTTGTCGTTTGATTGATAATACCTTCCGCTTGAGACACTGCATTTGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTGGCaa                                                                                                                                  >  1:162982/1‑141 (MQ=255)
    aTCTGGGTTAAGCGTTGGATTTGTCGTTTGATTGATAATACCTTCCGCTTGAGACACTGCATTTGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATAcc                                                                                                                              <  2:455624/141‑1 (MQ=255)
    aTCTGGGTTAAGCGTTGGATTTGTCGTTTGATTGATAATACCTTCCGCTTGAGACACTGCATTTGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATAcc                                                                                                                              >  2:282248/1‑141 (MQ=255)
     tCTGGGTTAAGCGTTGGATTTGTCGTTTGATTGATAATACCTTCCGCTTGAGACACTGCATTTGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCt                                                                                                                             >  2:7517/1‑141 (MQ=255)
          gTTAAGCGTTGGATTTGTCGTTTGATTGGTAATACCTTCCGCTTGAGACACTGCATTTGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGttt                                                                                                                        <  1:446260/141‑1 (MQ=255)
            tAAGCGTTGGATTTGTCGTTTGATTGATAATACCTTCCGCTTGAGACACTGCATTTGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCa                                                                                                                      >  1:236594/1‑141 (MQ=255)
                 gTTGGATTTGTCGTTTGATTGATAATACCTTCCGCTTGAGACACTGCATTTGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTa                                                                                                                 >  1:351703/1‑141 (MQ=255)
                      aTTTGTCGTTTGATTGATAATACCTTCCGCTTGAGACACTGCATTTGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCAt                                                                                                            >  2:414444/1‑141 (MQ=255)
                                tgattgatAATACCTTCCGCTTGAGACACTGCATTTGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTc                                                                                                  <  2:410254/141‑1 (MQ=255)
                                       taataCCTTCCGCTTGAGACACTGCATTTGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACAtttt                                                                                           >  1:475290/1‑141 (MQ=255)
                                               tCCGCTTGAGACACTGCATTTGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCAttt                                                                                   >  1:432114/1‑141 (MQ=255)
                                                    ttGAGACACTGCATTTGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGttt                                                                              <  2:245158/141‑1 (MQ=255)
                                                                  ttGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTg                                                                >  1:357832/1‑141 (MQ=255)
                                                                    gTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTa                                                              >  2:17782/1‑141 (MQ=255)
                                                                     tataTGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAg                                                             <  1:441190/141‑1 (MQ=255)
                                                                      atatGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGc                                                            >  1:171929/1‑141 (MQ=255)
                                                                                tGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTtgt                                                  >  2:440432/1‑141 (MQ=255)
                                                                                 gCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTtgtg                                                 >  2:234934/1‑141 (MQ=255)
                                                                                      tgggCCTCAGCATCATGATATTTCTCACTTGCTTTGACAGTATCTTTGTCTGCACTACCTTGTTTTAAGTTACTCATTCCTGTATTGAAATTTTGTGTATTTCGTTTAGTTTGTTCACCTGTAGCTACCGCTGGTGCttga                                            <  2:140828/139‑1 (MQ=255)
                                                                                      tCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCttga                                            <  1:7517/141‑1 (MQ=255)
                                                                                      tCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCttga                                            <  2:357832/141‑1 (MQ=255)
                                                                                       cGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCACTACCTTGTTTCAAGTTACTAATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCttgat                                           >  1:20200/1‑141 (MQ=255)
                                                                                       cGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCttgat                                           >  2:501381/1‑141 (MQ=255)
                                                                                       cGGCATCAGCATCATGATAGTTCTCACGTGCTTTGAAAGTATCTTTTTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACGTTTTGTGCATTTTGTTTGGTTTGTCCAACTGTATCTAACGCTTGTGCttgaa                                           <  2:124285/141‑2 (MQ=255)
                                                                                             cagcatcaTGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGTTTGAttt                                     >  1:196241/1‑141 (MQ=255)
                                                                                              agcatcaTGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTg                                    <  1:400997/141‑1 (MQ=255)
                                                                                                catcatGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTGGt                                  <  1:100554/141‑1 (MQ=255)
                                                                                                 atcatGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTGGtt                                 >  2:168377/1‑141 (MQ=255)
                                                                                                        tAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTGGTTACGTAAt                          >  1:121266/1‑141 (MQ=255)
                                                                                                          gTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTGGTTACGTAATGc                        <  2:140396/141‑1 (MQ=255)
                                                                                                              tcACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTGGTTACGTAATGCATCa                    <  1:153654/141‑1 (MQ=255)
                                                                                                                        tGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTGGTTACGTAATGCATCACGTTGTGGtt          <  1:226455/141‑1 (MQ=255)
                                                                                                                         gACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTGGTTACGTAATGCATCACGTTGTGGTTg         >  1:394992/1‑141 (MQ=255)
                                                                                                                          aCAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTGGTTACGTAATGCATCACGTTGTGGTTGa        <  1:367376/141‑1 (MQ=255)
                                                                                                                           cAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTGGTTACGTAATGCATCACGTTGTGGTTGAt       <  2:452768/141‑1 (MQ=255)
                                                                                                                           cAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTGGTTACGTAATGCATCACGTTGTGGTTGAt       <  2:454635/141‑1 (MQ=255)
                                                                                                                            aGTATCTTTGTTGGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTGGTTACGTAATGCATCACGTTGTGGTTGAtt      <  2:162985/141‑1 (MQ=255)
                                                                                                                            aGTATCTTTGTTGGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTATAGCTAACGCTTGTGCTTGATTGATTTGGTTACGTAATGCATCACGTTGTGGTTGAtt      <  2:162982/141‑1 (MQ=255)
                                                                                                                               aTCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTGGTTACGTAATGCATCACGTTGTGGTTGATTTaa   >  2:85957/1‑141 (MQ=255)
                                                                                                                               aTCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTGGTTACGTAATGCATCACGTTGTGGTTGATTTaa   <  1:335790/141‑1 (MQ=255)
                                                                                                                                tCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTGGTTACGTAATGCATCACGTTGTGGTTGATTTaaa  <  2:160979/141‑1 (MQ=255)
                                                                                                                                tCTTTGGTTGCAATCCCTTGTTTCGAGTTACTCATTGCTCTCTTCACTTTTTGTGCATTTTGTTTAGTTGTTACATCTGTAGCTATCGCCTGTGCTTAACTGATTTGGTTACGTAATGCATGACGTGATGGTTGTTTTaaa  <  2:15712/141‑1 (MQ=255)
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TTTCATCTGGGTTAAGCGTTGGATTTGTCGTTTGATTGATAATACCTTCCGCTTGAGACACTGCATTTGTATATGCTGTTTGCTTATCGGCATCAGCATCATGATAGTTCTCACTTGCTTTGACAGTATCTTTGTTTGCAATACCTTGTTTCAAGTTACTCATTGCTGTATTCACATTTTGTGCATTTTGTTTAGTTTGTTCAACTGTAGCTAACGCTTGTGCTTGATTGATTTGGTTACGTAATGCATCACGTTGTGGTTGATTTAAA  >  CP000730/1478035‑1478303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑