Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 119,853 C→A 38.7% G254W (GGG→TGG)  yjbB ← possible PnaS family phosphate:sodium (Na+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730119,8530CA38.7% 56.3 / 54.1 54G254W (GGG→TGG) yjbBpossible PnaS family phosphate:sodium (Na+) symporter
Reads supporting (aligned to +/- strand):  ref base C (17/16);  new base A (13/8);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 5.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

TTGAATCAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCG  >  CP000730/119716‑119977
                                                                                                                                         |                                                                                                                            
ttGAATCAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACGAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGac                                                                                                                           <  1:385415/141‑2 (MQ=255)
 tGAATCAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGAtt                                                                                                                          >  1:10340/1‑141 (MQ=255)
      cAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGtt                                                                                                                     <  2:442099/141‑1 (MQ=255)
        aaGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTaa                                                                                                                   <  1:327440/141‑1 (MQ=255)
             tttGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATg                                                                                                              <  2:5447/141‑1 (MQ=255)
              ttGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCCAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGa                                                                                                             >  1:490225/1‑141 (MQ=255)
                 ttATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACgt                                                                                                          >  2:324873/1‑141 (MQ=255)
                    tGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTa                                                                                                       >  2:319988/1‑141 (MQ=255)
                       tGAAGATACCATGTGATCCTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAACCCCAACTGGCAATAAAATTGTGAAGATAATTACTCCGATTAAGTTAAAGATGACGTGTACaa                                                                                                    <  2:364758/141‑1 (MQ=255)
                          aGATAACATGTGATACTGCAATAGTCATCGATGGTAGTAAGTGACATAAATCTTGTAAGAAAATAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACACAGATTAAGTTAAAGATGACGTGTACAAgcg                                                                                                 >  2:269318/1‑141 (MQ=255)
                             tACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGcgccg                                                                                              <  1:378500/141‑1 (MQ=255)
                                    tgATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGttt                                                                                       <  2:489428/141‑1 (MQ=255)
                                     gATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGtttt                                                                                      <  2:70386/141‑1 (MQ=255)
                                       tACTGNAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGc                                                                                    <  1:200958/141‑1 (MQ=255)
                                         cTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAg                                                                                  >  1:464985/1‑141 (MQ=255)
                                             aattgTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCGAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGAt                                                                              <  2:117699/137‑1 (MQ=255)
                                              aTCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGAtt                                                                             >  2:190894/1‑141 (MQ=255)
                                                 gTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAg                                                                          <  2:406297/141‑1 (MQ=255)
                                                   cATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGcc                                                                        >  1:228800/1‑141 (MQ=255)
                                                   cATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGcc                                                                        >  1:103509/1‑141 (MQ=255)
                                                    aTCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCg                                                                       <  1:42297/141‑1 (MQ=255)
                                                     tCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCgg                                                                      >  2:67102/1‑141 (MQ=255)
                                                           gTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAAc                                                                <  1:339427/141‑1 (MQ=255)
                                                              ttAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAg                                                             >  1:176467/1‑141 (MQ=255)
                                                              ttAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAg                                                             <  1:67102/141‑1 (MQ=255)
                                                              ttAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAg                                                             >  2:97459/1‑141 (MQ=255)
                                                                 aGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTa                                                          >  1:99241/1‑141 (MQ=255)
                                                                    gCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGa                                                       >  1:123031/1‑141 (MQ=255)
                                                                      cATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATa                                                     >  2:46871/1‑141 (MQ=255)
                                                                        tAAATCTTGTAACAAACTCATCAAATGACGCACCACTGGCAAGAAAATGGTGAAGATAATTACCCCGATTAAGTTAAAGGTGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGc                                                   <  1:359201/141‑1 (MQ=255)
                                                                          aaTCTTGTAACAGACTAATCAAATGACTCACAACGGGCAAGAAAATTGTGACGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTg                                                 >  2:8466/1‑141 (MQ=255)
                                                                            tCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTa                                               >  1:377839/1‑141 (MQ=255)
                                                                             cTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTaa                                              <  1:495890/141‑1 (MQ=255)
                                                                                gTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCg                                           >  1:462740/1‑141 (MQ=255)
                                                                                gTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGCTGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCg                                           >  1:253100/1‑141 (MQ=255)
                                                                                 tAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGt                                          <  1:97459/141‑1 (MQ=255)
                                                                                         tAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCaa                                  <  1:203222/141‑1 (MQ=255)
                                                                                          aaTCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAAt                                 <  2:328533/141‑1 (MQ=255)
                                                                                           aTCAAATGAATCACAACTGGCAAGAAAAGAGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCTTGGTACCAAtt                                >  2:234564/1‑140 (MQ=255)
                                                                                            tCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGt                               <  2:315786/141‑1 (MQ=255)
                                                                                              aaaTGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTa                             <  1:392571/141‑1 (MQ=255)
                                                                                              aaaTGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTa                             >  2:389311/1‑141 (MQ=255)
                                                                                              aaaTGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTa                             >  2:294258/1‑141 (MQ=255)
                                                                                                    aTCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCt                       >  2:455273/1‑141 (MQ=255)
                                                                                                              gCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAg             >  2:460810/1‑141 (MQ=255)
                                                                                                              gCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAg             >  2:13999/1‑141 (MQ=255)
                                                                                                               cAAGAAAATTGTGAAGATAATTACCCAGATTAACTTAAAGATGACGTGTACAATCGCCGCACGGTTTGCAGCGATTGAGCCGGCCAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTACTAGATAGTAACACAgg            >  2:390785/1‑141 (MQ=255)
                                                                                                                     aaTGGTGAAGATAATTACCCCTATTAAGTTAAAGATGACGTGTACAAGCGCCGCACTTTTTGCAGCAATTGAGCCGGCTAAACTAGCTAAGATAACTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgc      <  2:378151/141‑1 (MQ=255)
                                                                                                                      aTTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgct     <  2:464985/141‑1 (MQ=255)
                                                                                                                      aTTGTGAAGATAATTACCCAGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgct     <  2:437801/141‑1 (MQ=255)
                                                                                                                       ttGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATtgctg    >  2:442406/1‑141 (MQ=255)
                                                                                                                         gtgAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGTCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCg  >  1:416662/1‑141 (MQ=255)
                                                                                                                         gtgAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCg  >  2:470828/1‑141 (MQ=255)
                                                                                                                         gtgAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGACGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCg  >  1:416685/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                            
TTGAATCAAAGTATTTGTTATGTTGAAGATACCATGTGATACTGCAATCGTCATCGCTGGTTTTAAGTGCCATAAATCTTGTAACAAACTAATCAAATGAATCACAACTGGCAAGAAAATTGTGAAGATAATTACCCCGATTAAGTTAAAGATGACGTGTACAAGCGCCGCACGTTTTGCAGCGATTGAGCCGGCTAAACTAGCTAAGATAGCTGTAATCGTGGTACCAATGTTATCGCCTAGTAACACAGGGATTGCTGCG  >  CP000730/119716‑119977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: