BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,471	A→G	25.6%	S27S (AGT→AGC)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,471	0	A	G	25.6%	196.6 / 75.5	137	S27S (AGT→AGC)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base A (54/47); new base G (19/16); total (74/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 141 total aligned reads displayed.

CGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGT  >  CP000730/36331‑36609

ctccAATTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                                                                            <  2:161173/139‑1 (MQ=255)

cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                                                                            <  2:536353/141‑1 (MQ=18)

cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                                                                            <  1:391525/141‑1 (MQ=40)

 cccATTTTTAATAATCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                                                                                                                           <  1:759316/140‑1 (MQ=255)

 cccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACCCCACGTTCCCTTAATATTTCAGGTATATCACGATAAc                                                                                                                                           <  1:449238/140‑1 (MQ=255)

  ccATTTGTAATAAGCTTTTTTTTTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATCATGAACGTTTACACCACGTTCCCTTAATATTTCAGTTATATCACGTTAACt                                                                                                                                          >  2:62384/1‑141 (MQ=11)

  ccATTTGTAATAAGCTTTTTTATGCTTTTTTTTTTTTTTTTGATATAAAATTGGGGCATATTCTTTAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCGTTCCCTTAATATTTCAGATATATCCCGATAACt                                                                                                                                          >  2:782907/1‑141 (MQ=11)

  ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                                                                          >  2:227140/1‑141 (MQ=255)

  ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                                                                          >  1:661798/1‑141 (MQ=11)

  ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                                                                                                          <  1:327425/141‑1 (MQ=18)

  ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                                                                                                          >  1:672210/1‑141 (MQ=25)

  ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTCCAGATATATCACCATAAca                                                                                                                                          >  1:103718/1‑140 (MQ=255)

   cagttGTAATAATCTTTTTTATGCTTTTTCTTTCAAATTTGATATAAAAGTGGTGCATATTCTTGAACCCAACGGTAGATCGGTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                                         <  1:748918/138‑1 (MQ=11)

   cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                                         >  1:387106/1‑141 (MQ=21)

   cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTAGGGCATATCCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCTGATATATCACGTTAACTc                                                                                                                                         >  1:159014/1‑141 (MQ=11)

   cATTTGTAATAAGCTTGTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                                         >  1:418040/1‑141 (MQ=17)

    aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATTTTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTACTATTTCAGATATATCACGCTAACTCa                                                                                                                                        >  2:540692/1‑141 (MQ=255)

    aTTTGTAATAAGCTTTTGTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                                                                                                                        <  1:680640/141‑1 (MQ=11)

     tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                                                                                       >  2:314489/1‑141 (MQ=255)

     tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                                                                                       <  2:710332/141‑1 (MQ=11)

     tttGTAATAAGCTCATTTATGCTTCTTCTTCCAAATTTGATATAAAATTGGTGCATATTCTTGAACCCAACGGTAGACCGATGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                                                                                       >  2:75087/1‑141 (MQ=11)

      ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGATCCCATCGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                                                      <  1:609789/141‑1 (MQ=11)

      ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                                                      >  1:246425/1‑141 (MQ=255)

      ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                                                      >  1:203371/1‑141 (MQ=11)

      ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                      <  1:304243/141‑1 (MQ=18)

      ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                      <  2:795412/141‑1 (MQ=21)

      ttGTAATAAGCTTGTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                      <  1:550557/141‑1 (MQ=17)

       tgcaataaGCTTTTTTATGCCTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                                     <  1:156535/138‑1 (MQ=11)

       tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGCTGAACGTTTACACCACGGTCTCTTAATATTTCGGATATATCACGATAGGTCaagg                                                                                                                                     >  1:335451/1‑139 (MQ=11)

       tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                                     >  1:800607/1‑141 (MQ=255)

       tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                                     <  2:331475/141‑1 (MQ=11)

       tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCGTTCCCTTAATATTTCCGATATATCACGTTAACTCAAtc                                                                                                                                     >  2:302540/1‑140 (MQ=255)

       tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                                                     <  1:125845/141‑1 (MQ=18)

       tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTGATATTTCAGATATATCACGATAACTCAATg                                                                                                                                     >  2:522802/1‑141 (MQ=255)

       tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACACAACGGTAGAGTGTTTGATGATGAGCGTTTACACCACGTTCCCTTAATATTTCAGGTATTTTACGATATATCAAtt                                                                                                                                     >  2:90471/1‑140 (MQ=14)

       tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAGTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGCTGAACGTTTTCATCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                                     <  1:704584/141‑1 (MQ=11)

        gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTTCACAGCGGCCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                                                                                    >  1:781269/1‑140 (MQ=11)

        gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTACTATTTCAGATATATCACGATAGCTCAATgc                                                                                                                                    >  1:316748/1‑140 (MQ=11)

        gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                                                                                    >  2:1219/1‑140 (MQ=255)

        gTAATAAGCTTATTTCAACTTTTTCTTCCGGATTTGGTATAGTTTTGTGGCGTTTTGTTTAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                                                                                    >  2:489090/1‑140 (MQ=2)

         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGTGCAATCACGATAGCTCAATgca                                                                                                                                   >  1:289353/1‑139 (MQ=11)

         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                   >  1:127246/1‑139 (MQ=11)

         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                   <  2:651498/141‑3 (MQ=11)

         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                   >  1:26382/1‑139 (MQ=255)

         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                   >  2:484158/1‑139 (MQ=255)

         taataaGCTTTTTTATCCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                   <  1:426204/141‑3 (MQ=11)

         taataaGCTTTTTGATGCTTTTTCTTCCAAATTTCATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGTACTTTTACACCACGATCCCTTAATATTTCAGATATATCACGATAACTCAATGta                                                                                                                                   <  1:325140/141‑1 (MQ=14)

          aataaGCTTTTTTTTTTTTTTTTTTCCAAAATTGAGATAAAATTGGGGCATATCCTTGAACCCAACGGTAGACCGTTGTATTATGAACGTTCCCACCACGTTCACCTAATATTTCATATTTATTACGATAACTCatttaaa                                                                                                                                  >  2:736036/1‑135 (MQ=2)

          aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGtaa                                                                                                                                  >  1:455946/1‑140 (MQ=255)

           ataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGaata                                                                                                                                 >  2:5688/1‑141 (MQ=17)

              aGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCt                                                                                                                              <  1:727624/141‑1 (MQ=39)

              aGCTTTTTTAGTCTTTTTCTTCCATATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCt                                                                                                                              <  2:694149/141‑1 (MQ=255)

                   ttttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAt                                                                                                                         <  1:42192/141‑1 (MQ=35)

                       ttttttttttttACAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGAACGTTGAATGATGAACGTATCCACTACGTTCCCTTAATTTTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagta                                                                                                                     >  2:61471/5‑141 (MQ=11)

                          cTTTTTCTTCCAAATTTGATACAAAATTTGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGcc                                                                                                                  <  1:837981/141‑1 (MQ=25)

                                  tcCAAATTTGATACAAACTTGGAAATGCTATTTTAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTa                                                                                                          >  1:830863/1‑141 (MQ=11)

                                  tcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTa                                                                                                          >  1:762679/1‑141 (MQ=35)

                                      aaTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGt                                                                                                      <  2:556495/141‑1 (MQ=35)

                                          tGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATa                                                                                                  <  1:271504/141‑1 (MQ=35)

                                                      ggggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTTCAGTGATAACATCCTTGTTa                                                                                      <  2:595522/141‑1 (MQ=17)

                                                      ggggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCGTAGATAGTAGCCAACGGCTACAGGGATAACATCCTTGTTa                                                                                      >  1:71894/1‑141 (MQ=17)

                                                       gggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaa                                                                                     >  1:18646/1‑141 (MQ=32)

                                                        ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaaa                                                                                    >  1:2874/1‑141 (MQ=32)

                                                         gCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACATTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAAt                                                                                   >  2:834053/1‑141 (MQ=17)

                                                             atTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTTCTTCCCGTTGTCTTAATATTTCTGATCTCTTACGATAACAAAAACTACATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGtt                                                                               <  1:401720/141‑1 (MQ=11)

                                                                   gAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATc                                                                         >  2:686882/1‑141 (MQ=32)

                                                                   gAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATc                                                                         >  1:255631/1‑141 (MQ=32)

                                                                   gAACCCAACGGGAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACATTGATAACATCCTTGTTAAATGGTTTATATc                                                                         >  2:284525/1‑141 (MQ=12)

                                                                     aCCCAAACGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTg                                                                       <  1:179927/141‑1 (MQ=17)

                                                                      cccAACGGTAGACCGTTGAATTCTGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTAGATCTGa                                                                      <  2:464436/141‑1 (MQ=12)

                                                                        cAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATGGTTTATATCTGaaa                                                                    <  1:547741/141‑1 (MQ=17)

                                                                             gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGtt                                                               >  1:470960/1‑141 (MQ=32)

                                                                             gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGtt                                                               >  1:470750/1‑141 (MQ=32)

                                                                             gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGtt                                                               <  2:762679/141‑1 (MQ=32)

                                                                                  ccGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATAc                                                          >  2:171911/1‑141 (MQ=32)

                                                                                     gtGAATGATGAACGGTTAAACCCAGTTCTCTTAAAATTACCGGTATATCACGATCGTTCAATGTCGATCTGGGATATTAGACGTGGGCCACAGGTAGAATAGCCTTGGTATATTGGTTATATCTGAAATAGTTCATACaga                                                       <  2:198023/140‑1 (MQ=2)

                                                                                      tGAATGATTAACGTTTACACCACGTGCCCTGAAGATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACagaa                                                      <  2:54295/141‑1 (MQ=255)

                                                                                               aaCGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCttt                                             <  1:614025/141‑1 (MQ=32)

                                                                                               aaCGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCttt                                             <  2:45716/141‑1 (MQ=32)

                                                                                                aCGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACGTAGTGGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCtttt                                            >  1:478394/1‑141 (MQ=255)

                                                                                                aCGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCtttt                                            >  2:691288/1‑141 (MQ=32)

                                                                                                  gTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTg                                          >  2:425974/1‑141 (MQ=32)

                                                                                                   tttACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGt                                         >  2:56874/1‑141 (MQ=32)

                                                                                                    ttACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGtt                                        >  2:149682/1‑141 (MQ=32)

                                                                                                       caccacGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTaaa                                     >  1:211831/1‑141 (MQ=32)

                                                                                                            cGTTCCCTTAATATTTCAGATATATCACGATCACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATtat                                >  2:765449/1‑141 (MQ=17)

                                                                                                                 ccTTAATAGTGCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACtat                           <  1:266046/141‑1 (MQ=17)

                                                                                                                   ttAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaa                         >  1:30189/1‑141 (MQ=33)

                                                                                                                    tAATATTTTATATAAATCACGATAGCTCAATGAATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATaaa                        <  1:412/141‑1 (MQ=11)

                                                                                                                    tAATATTTCAGATATAGCACGATAACTCAATGTATATCTTAGATAATAGCCAACTGCTACAGTTATAATATCCTTGTTAAATTGTTTATATCTCAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaaa                        <  2:247909/141‑1 (MQ=11)

                                                                                                                        atgtCAGCTATATCACGACAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCa                    <  1:108250/138‑1 (MQ=255)

                                                                                                                        atTTCCGATATATCACGATAACTCCATGTACATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCa                    <  1:358286/141‑1 (MQ=255)

                                                                                                                             aGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACttt               >  1:102941/1‑141 (MQ=35)

                                                                                                                                tatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCa            <  1:83144/141‑1 (MQ=35)

                                                                                                                                tatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCa            >  2:333053/1‑141 (MQ=34)

                                                                                                                                taaatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCa            <  2:699634/138‑1 (MQ=21)

                                                                                                                                      aCGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGaa      >  1:96305/1‑141 (MQ=35)

                                                                                                                                      aCGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACACAAGACTCCTTTTTATTAAAATTGTACTATAAATTAACCTTGGGACCAGaa      >  1:195328/1‑141 (MQ=11)

                                                                                                                                       cGATAACTCAATGTATATCTTAGATCGTACCTATCGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCACCAGAAc     >  1:404794/1‑141 (MQ=11)

                                                                                                                                          tAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt  <  1:143588/141‑1 (MQ=37)

CGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGT  >  CP000730/36331‑36609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`