Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 909,616 C→A 10.4% intergenic (‑539/‑495) ear ← / → USA300HOU_0868 Ear protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730909,6160CA10.4% 183.6 / 3.4 73intergenic (‑539/‑495)ear/USA300HOU_0868Ear protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (45/19);  new base A (3/6);  total (48/25)
Fisher's exact test for biased strand distribution p-value = 5.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

ATACATATATCTTGATAACATAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGCCCTTGAACTTCCTTGATAATCTGGCTAATTATTCCTGCTAAA  >  CP000730/909477‑909754
                                                                                                                                           |                                                                                                                                          
atacatgttGCTTGGTATCGTAGTGTTGTTATAGTCGCTACGCCAGTGATACTAGTTACTGGGTGGTTGTTTTGATGTTATAGTATGAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCa                                                                                                                                           <  2:665704/141‑1 (MQ=255)
atacatataTCTTGATAACATAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCa                                                                                                                                           <  1:383835/141‑1 (MQ=255)
     tatatCTTGATAACATAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCGAACCACACCACCTATTACTTTAGGAGAGTGGTTGTTTTCAAGAGAaaaaaa                                                                                                                                      >  2:467348/1‑141 (MQ=255)
         tCTTGATAACATAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAggg                                                                                                                                  >  2:247226/1‑141 (MQ=255)
            tGATAACATAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCAt                                                                                                                               >  1:411831/1‑141 (MQ=255)
            tGATAACAGAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGATGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTTAAGGGCAAAAAAAGGGCAt                                                                                                                               <  1:469506/141‑1 (MQ=255)
              aTAACATAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATaa                                                                                                                             <  1:372421/141‑1 (MQ=255)
               tAACATAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAAt                                                                                                                            >  1:786061/1‑141 (MQ=255)
                aaCATAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAAtt                                                                                                                           >  1:802839/1‑141 (MQ=255)
                 aCATAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAAttt                                                                                                                          >  2:496773/1‑141 (MQ=255)
                  cATAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAAtttt                                                                                                                         >  1:30119/1‑141 (MQ=255)
                   aTAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAGCGGCACAAAAAGGGCATAATTTTg                                                                                                                        >  2:56802/1‑141 (MQ=255)
                     agagTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGaa                                                                                                                      >  1:662854/1‑141 (MQ=255)
                         ttgttATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACaa                                                                                                                  >  1:666046/1‑141 (MQ=255)
                             tataGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAggg                                                                                                              <  1:48631/141‑1 (MQ=255)
                              ataGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAggga                                                                                                             >  2:751321/1‑140 (MQ=255)
                                aGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCag                                                                                                           >  2:324815/1‑140 (MQ=255)
                                aGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCaa                                                                                                           >  1:805239/1‑141 (MQ=255)
                                     cTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTTTTCCTGGTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTa                                                                                                      >  1:649773/1‑141 (MQ=255)
                                       agggcACTCAAACTAGTTACTGGGGGGTTGATAATCTGTTATATTAAAAAGGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTAAAGGGAAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATg                                                                                                    <  1:154193/137‑1 (MQ=255)
                                       aagacacacaAACTAAATAATGGGTGGGTGTTTATATACTAAATTATAAATGATCAAACCACACAAACAATTAATTTAGGAGTGTGGTTGTTAAAAAAAAAAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATg                                                                                                    <  2:80018/137‑1 (MQ=255)
                                       aCGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTGATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATg                                                                                                    <  1:154179/141‑1 (MQ=255)
                                        cGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGa                                                                                                   >  1:773593/1‑141 (MQ=255)
                                         gCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGaa                                                                                                  >  2:475461/1‑141 (MQ=255)
                                          ctacacATACTAGGTAAAGGGTGGTTGTTGATAGGTTATATTATAAATGCTCAAACCACGCTACCTTCTACTTCGGGAGCGGGCGTGGATTCGAGGACACAATAAGGCCATAATTTTGAAACAAAGGTCAAGGGTATGaga                                                                                                 >  2:299810/6‑139 (MQ=255)
                                          ccACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGaat                                                                                                 >  2:208049/1‑141 (MQ=255)
                                          ccACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGaat                                                                                                 >  1:372635/1‑141 (MQ=255)
                                          ccACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGaat                                                                                                 >  2:508220/1‑141 (MQ=255)
                                          ccACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGACAAAAAAAGGGCATAAGTTTGGAACAAAGGACAAGGGTATGaat                                                                                                 >  2:108789/1‑141 (MQ=255)
                                          ccACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAAACACACAACCTAATAATTTAGGCGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAGAGGGCACGGGTATGaat                                                                                                 >  2:576579/1‑141 (MQ=255)
                                          ccACTCATACCAGGTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCCAACAATCTATTATATTAGTCGTTTGTTTGTTTTTTGGCACAGAATAGAGGCATAATTTTGAAACAAAGGGCACGGGTATGaat                                                                                                 >  2:300180/1‑141 (MQ=255)
                                           cACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCCAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGaata                                                                                                >  2:565974/1‑141 (MQ=255)
                                           cACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCAAAATTTTGAAACAAAGGGCAAGGGTATGaaaa                                                                                                >  2:793475/1‑139 (MQ=255)
                                           cACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGAAAAAAAAGGGCATAATTTTGAAACAAATGGCAAGGGTATGaata                                                                                                >  2:473429/1‑141 (MQ=255)
                                            aCTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGaataa                                                                                               <  2:599779/141‑1 (MQ=255)
                                                    tAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTAt                                                                                       <  2:224785/141‑1 (MQ=255)
                                                        tagtGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGAAAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTg                                                                                   <  2:204382/138‑1 (MQ=255)
                                                        tACTGGGTTGTTGGTTTTATGGTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTAAAAAAGAAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTg                                                                                   <  2:434191/141‑1 (MQ=255)
                                                        tACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTg                                                                                   <  2:185022/141‑1 (MQ=255)
                                                            gggTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAACTTATCTTGaaaa                                                                               >  1:678862/1‑141 (MQ=255)
                                                            gggTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGaaaa                                                                               >  2:123544/1‑141 (MQ=255)
                                                              gtggtTGTTTTTATGTTATATTATAAATGATCAAACCACACCATCTATTAATTTAGGAGTGTTGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAAtt                                                                             <  2:296163/141‑1 (MQ=255)
                                                              gtggtTGTTTGTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAAtt                                                                             <  1:120829/141‑1 (MQ=255)
                                                               tggtTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAAttt                                                                            >  2:614026/1‑141 (MQ=255)
                                                                  ttttttttATTTTATATTATAAATGATCAAACTAAACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTtat                                                                         <  2:630298/138‑1 (MQ=255)
                                                                        ttATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGAGAAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGc                                                                   <  2:716793/141‑1 (MQ=255)
                                                                                 attataAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATaaa                                                          >  1:5816/1‑140 (MQ=255)
                                                                                      aaaTGATCAAACCACACCACCTATTAATTTAGGAACGTGGTTGTTTTCACGGGCAAAAAAAGGGCATAATTTTGAAACACAGGGCAAGGGTATAAATAATAAATTATCTTGAAAATTTTATAAATGCATATATCACAATGt                                                     >  2:63130/1‑141 (MQ=255)
                                                                                       aaTGATCAAACCACATCAACTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGtt                                                    <  2:482134/141‑1 (MQ=255)
                                                                                         tGATCAAACCACACCACCTATTAATTTAGGAGTGGGGTTGTTTTCCAGCCATAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGtttt                                                  <  1:605575/141‑1 (MQ=255)
                                                                                          gATCAAACCACACCACCTATTAATTTTGGTGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGCAACAAAGGACAAGGGTATGAATACTAAATTATCTTGGAAATTTTATAAATGCATATATAACAAAGATTTg                                                 >  2:141266/1‑141 (MQ=255)
                                                                                          gATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTg                                                 >  2:21084/1‑141 (MQ=255)
                                                                                          gATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTg                                                 >  2:525327/1‑141 (MQ=255)
                                                                                             cAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGAtt                                              >  2:101658/1‑141 (MQ=255)
                                                                                               aaCCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGc                                            >  1:491138/1‑141 (MQ=255)
                                                                                                accacaccacCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATATTATAAAAGCAAAAATAACAATGTTTAGATTGcc                                           >  1:220443/1‑141 (MQ=255)
                                                                                                 ccacaccacCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAAAAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTCATAAATGCATATATAACAATGTTTTGATTgtct                                          >  1:836286/1‑138 (MQ=255)
                                                                                                   acaccacCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGCCCtt                                        <  1:496773/141‑1 (MQ=255)
                                                                                                   acaccacCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGCCCtt                                        >  1:522247/1‑141 (MQ=255)
                                                                                                        accTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGCCCTTGAACt                                   >  1:156822/1‑141 (MQ=255)
                                                                                                                        gtgtGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATCTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGCCCTTGAACTTTCTTGATAATCTGGc                   <  1:465056/141‑1 (MQ=255)
                                                                                                                         tgtgGTTGTTTTCAAGGGCAAAAAAAGGGCACAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGCCCTTGAACTTCCTTGATAATCTGGCt                  <  1:288399/141‑1 (MQ=255)
                                                                                                                          gtgGTTGTGTTCAAGGGCAAAAAAAGGGCATATTTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAACAGTTTATAAATGCATATATAACAATGTGTTGTTTGCCCTTGACCTTCCTTGATAAGCTGGCTa                 >  2:774537/1‑141 (MQ=255)
                                                                                                                             gttgttTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGCCCTTGAACTTCCTTGATAATCTGGCTAAtt              >  1:105567‑M2/1‑139 (MQ=255)
                                                                                                                              ttgttTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGCCCTTGAACTTCCTTGATAATCTGGCTAAttt             <  1:400601‑M2/141‑4 (MQ=255)
                                                                                                                               tgttTTCAAGGGAAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGCCCTTGAACTTCCTTGATAATCTGGCTAAttat            >  2:455032‑M2/1‑137 (MQ=255)
                                                                                                                                  tttCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTCTAAATGCATATATACAAATGTTTTGATTGCCCTTTAACTTCCTTGATAATCTGACTAAttattcc         >  2:805239‑M2/1‑134 (MQ=255)
                                                                                                                                     cAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGCCCTTGAACTTCCTTGAGAATCTGGCTAAttattcctgc      >  1:433247‑M2/1‑131 (MQ=255)
                                                                                                                                      aaGTGCAAAAAAAGTGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGCCCTTGAACTTCCTTGATAATCTGGCTAAttattcctgct     >  2:761656‑M2/1‑130 (MQ=255)
                                                                                                                                       aGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGCCCTTGAACTTCCTTGATAATCTGGCTAAttattcctgcta    <  2:649999‑M2/141‑13 (MQ=255)
                                                                                                                                        gggCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGCCCTTGAACTTCCTTGATAATCTGGCTAAttattcctgctaa   >  1:208049‑M2/1‑128 (MQ=255)
                                                                                                                                         ggCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGCCCTTGAACTTCCTTGATAATCTGGCTAAttattgttgctaat  <  1:305474‑M2/141‑15 (MQ=255)
                                                                                                                                         ggCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGCCCTTGAACTTCCTTGATAATCTGGCTAAttattcctgctaaa  >  2:449284‑M2/1‑127 (MQ=255)
                                                                                                                                           |                                                                                                                                          
ATACATATATCTTGATAACATAGAGTTGTTATAGTCGCTACGCCACTCATACTAGTTACTGGGTGGTTGTTTTTATGTTATATTATAAATGATCAAACCACACCACCTATTAATTTAGGAGTGTGGTTGTTTTCAAGGGCAAAAAAAGGGCATAATTTTGAAACAAAGGGCAAGGGTATGAATAATAAATTATCTTGAAAATTTTATAAATGCATATATAACAATGTTTTGATTGCCCTTGAACTTCCTTGATAATCTGGCTAATTATTCCTGCTAAA  >  CP000730/909477‑909754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: