Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,990,921 T→C 6.0% K70K (AAA→AAG glnQ ← glutamate ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,990,9210TC 6.0% 198.1 / 3.4 74K70K (AAA→AAGglnQglutamate ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base T (39/30);  new base C (2/3);  total (41/33)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

ATGTAATTCATCGTTATTATCTTTCTTTAATAATTTAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACCAGATGGACCTATTATTGCTACTACTTCCCCTTGATTGA  >  CP000730/1990783‑1991054
                                                                                                                                          |                                                                                                                                     
aTGTAATTCATCGTTATTATCTTTCTTTAATAATTTAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCttt                                                                                                                                     <  2:558621/141‑1 (MQ=255)
 tGTAATTCATCGTTATTATCTTTCTTTAATAATTTAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCtttt                                                                                                                                    >  2:417470/1‑141 (MQ=255)
      ttCATCGTTATTATCTTTCTTTAATAATTTAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGt                                                                                                                               >  1:66061/1‑141 (MQ=255)
      ttCATCGTTATTATCTATCTTTAATAATTTAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCCTTTTCCGt                                                                                                                               >  1:417235/1‑141 (MQ=255)
       tCATCGTTATTATATTACCTTCCTGATTTAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGtt                                                                                                                              >  2:181570/1‑141 (MQ=255)
          tCGTTATTATCTTTCTTTAATAATTTAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAg                                                                                                                           >  1:595855/1‑141 (MQ=255)
           cGTTATTATCTTTCTTTAATAATTTAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGt                                                                                                                          >  1:713547/1‑141 (MQ=255)
           cGTTATTATCTTTCGTTAATAATTTAGGAGCTAAAATAATAGTATCGACAACTTTTTTATGTGGCAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGt                                                                                                                          >  2:752040/1‑141 (MQ=255)
                   tctttcttTAATAATTTAGGAGCTAACATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTACGTCATTGCCt                                                                                                                  <  1:606971/141‑1 (MQ=255)
                    ctttctttAATAATTTAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCTGTTTGTTTATTGCCtt                                                                                                                 >  2:653828/1‑141 (MQ=255)
                     tttctttAATAATTTAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGCAGTTTTGAAATACCATACCCATTGTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTc                                                                                                                <  2:604954/141‑1 (MQ=255)
                     tttctttAATAATTTAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTc                                                                                                                >  1:763253/1‑141 (MQ=255)
                      ttctttATAACTATAGGATCTAAAATAATTTTATCGACAACTTTTTTATGTTGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTTACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCa                                                                                                               <  2:658900/141‑1 (MQ=255)
                      ttctttAATAATTTAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGTCTTTTTTTTTCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCa                                                                                                               >  2:393286/1‑141 (MQ=255)
                       tctttAATAATTTAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCCTTTTCCGTTAAGTCATTGCCTTCaa                                                                                                              <  2:661497/141‑1 (MQ=255)
                       tctttAATAATTTAGCAGCGAAAATAATATTATCTACAACTTATTTATGTGGAAATAGGTTCAAATTGTGAAATTCCATACCCGTTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCCTTGCCTTCaa                                                                                                              >  1:625675/1‑141 (MQ=255)
                          ttAATAATTTCGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCaaaaa                                                                                                           <  2:1164/141‑1 (MQ=255)
                           taataaTTTAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTTCTTTATTTTATTGCCTTCAAAAAt                                                                                                          >  2:566637/1‑141 (MQ=255)
                                   tAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGAc                                                                                                  >  1:667124/1‑141 (MQ=255)
                                   tAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATAACTTGAc                                                                                                  >  1:290885/1‑141 (MQ=255)
                                    aGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGAcc                                                                                                 <  1:591678/141‑1 (MQ=255)
                                     ggAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCt                                                                                                >  1:7511/1‑141 (MQ=255)
                                       aGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCttt                                                                                              <  1:117677/141‑1 (MQ=255)
                                          tAAAATAATATTATCGACACCTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTtagt                                                                                           <  1:31305/141‑1 (MQ=255)
                                          tAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTGAAAAATCACTTGACCTTtagt                                                                                           >  1:715100/1‑141 (MQ=255)
                                          tAAAATAATATTATCGACAACTTCTTCATTTGGAAATAGGTTGTAGATCACTTACACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTtagt                                                                                           <  1:819341/141‑1 (MQ=255)
                                          aaaaataaaatTATCGACAACTTTTTTATGTGGAAATTGGTTGAAGTTTTGAAATACCATACCCATTTTTCGACGTAGTTTATCTAATTGTGTCCCTTTTTCCGTTAAGAAAAAAAAAAAAAAAATCACTTGACCTTtagt                                                                                           <  2:443270/140‑1 (MQ=255)
                                                    ttatCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTTTTTTTTTTTTTTTCCTTAAAAAGTAGCTTTACTTTTTGTAGGTACtttt                                                                                 >  2:248913/1‑139 (MQ=255)
                                                     tatCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCta                                                                                >  1:763229/1‑141 (MQ=255)
                                                      atCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTGCTTCtaa                                                                               >  2:833203/1‑141 (MQ=255)
                                                      atCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTGACGTAGTTTATCTACTTGTGTCCCCTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCtaat                                                                              >  1:407532/1‑141 (MQ=255)
                                                      atCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTACAATACCATACCAATTTTTGGACGACGTTTATCTCCTTGTGTCCCTTTTTCCGTTATGTTATTGGCTTCAAAAATCACTGAACCTTTAGTACGTACttatca                                                                               >  2:35350/1‑137 (MQ=255)
                                                       tCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTTACTTAGTTTATCTACTGGTGTCGCTTTTTGAGTTAGTTCATTTACTTGGAAAATAACTTGACCTTTTGTGTGTACTTCtatt                                                                              >  2:232909/1‑139 (MQ=255)
                                                             aCTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCTGTGAAGTTCTTGCCGACAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTc                                                                        <  2:720189/141‑1 (MQ=255)
                                                                tttttATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTGATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCACAAATCACTTTACCTTTAGTAGGTGCTTCTAAAAACTTCATa                                                                     >  1:319262/1‑141 (MQ=255)
                                                                  tttATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACa                                                                   >  2:75126/1‑141 (MQ=255)
                                                                    tATGTGGAAATAGGTTGAAGTTTTGAAATTCCATACCCTTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCt                                                                <  2:150132/141‑1 (MQ=255)
                                                                     aTGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCt                                                                >  2:372467/1‑141 (MQ=255)
                                                                     aTGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCt                                                                <  1:52768/141‑1 (MQ=255)
                                                                        tgGAAATAGGTTGAGGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTaa                                                             >  2:305112/1‑141 (MQ=255)
                                                                         ggAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAAc                                                            <  2:603892/141‑1 (MQ=255)
                                                                          gAAATAGGTTGAAGTTTTGAAATACTCTACCCTTTTCTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACa                                                           <  1:19297/141‑1 (MQ=255)
                                                                             aTAGGTGAAATTTTTGAAATACCTTACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCCTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATg                                                        <  1:387128/141‑1 (MQ=255)
                                                                                  ttGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACtt                                                   >  1:756995/1‑141 (MQ=255)
                                                                                  ttGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGAACTTTATTAGGGACTTCTAATAAATTCATACATCTTAACAATGTACtt                                                   >  1:229955/1‑141 (MQ=255)
                                                                                   tGAAGTTTTGAAATACCATACCCATTTTTTAACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACttt                                                  <  1:229965/141‑1 (MQ=255)
                                                                                        ttttGAAATACCATACCCATTTTTTTTTTTTTTTTAACTACATGTGTCCCTTATTCCGTTAAGTCCTTGCCCTCAAAAATCACTTGACCCTTATTCGGTCCTTCTAATAAATTCATACATCTTAACAATGTACTTTTAcct                                             >  2:633504/1‑140 (MQ=255)
                                                                                        ttttGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCa                                             >  1:807957/1‑141 (MQ=255)
                                                                                        ttttGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCa                                             >  1:199891/1‑141 (MQ=255)
                                                                                          ttGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATATATCTTTACATTGTACTTTTACCaat                                           >  1:440902/1‑139 (MQ=255)
                                                                                          ttGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTACCAATGTACTTTTACCCCt                                           >  2:296432/1‑141 (MQ=255)
                                                                                          ttGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACt                                           <  2:717858/141‑1 (MQ=255)
                                                                                           tGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAACTTCATACATCTTAACAATGTACTTTTACCACTa                                          <  2:7511/141‑1 (MQ=255)
                                                                                             aactaccataccCCTTTTTTGACGGAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTAcc                                        <  1:22702/138‑1 (MQ=255)
                                                                                             aaaTACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTAcc                                        <  1:219700/141‑1 (MQ=255)
                                                                                                 accataccCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACCAGAt                                    >  1:656152/1‑141 (MQ=255)
                                                                                                  ccataccCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTAATTTTACCACTACCAGATg                                   >  2:11508/1‑141 (MQ=255)
                                                                                                      accCATTTTTTGACTTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTCGGTACTTCTAATAAATTCATACATCTTATCATTTTATTTTTACCCCTACCAGATGGAcc                               >  2:724070/1‑141 (MQ=255)
                                                                                                      accCATTTTTTGACCTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTGAGTAGGTACTTCCAATAAATTCATACATCTTAACAATGTACTTTTACCACTCCCAGATGTAcc                               >  2:574463/1‑141 (MQ=255)
                                                                                                          cttttttGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACCAGATGGACCtatt                           <  2:643804/140‑1 (MQ=255)
                                                                                                          aTTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACCAGATGGACCtatt                           <  1:114023/141‑1 (MQ=255)
                                                                                                          aTTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACCAGATGGACCtatt                           <  1:219002/141‑1 (MQ=255)
                                                                                                           ttttttttttttttttATCTACTTGTGTCCCTTTTTCCTTTAATTCATTGCCTTCAAAAATCACTTGACCTTTTTTAGGTACTTCTAATAAATTTATACTTCTTTTTAATGTACTTTTACCCCTACCACATGGACTtattt                          >  2:823688/14‑140 (MQ=255)
                                                                                                           ttttttGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACAAGATGGACCtattt                          >  2:468351/1‑140 (MQ=255)
                                                                                                            tttttGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACCAGATGGACCtattat                         <  1:181570/141‑1 (MQ=255)
                                                                                                                 ttttttttttATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAATTCACTTTCGTTTTAGTAGGTACTTCTTATGTATTCTTACATCTTAACAATGTACTTCTCCCCCCGCTAGATGCACCTATTTTTGcta                    >  2:546109/8‑141 (MQ=255)
                                                                                                                 gACGTAGTTTATCTACTTGTGTCCCTTTTTTTTTTTTTTTTTTGCCTTCAAAAATCACTTGACTTTTAGTAGGTGCTTCTAATATATTCATAGATCTTAACAATGTCCTTTTTTTACTACCAGATGGACTTATTATTgttt                    >  2:815581/1‑138 (MQ=255)
                                                                                                                   cGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACCGGATGGACCTATTATTGctact                  <  2:181907/141‑1 (MQ=255)
                                                                                                                          tATCTACTTGTGTCCCCTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACCAGATGGACCTATTATTGCTACTACTTccc           <  2:407532/141‑1 (MQ=255)
                                                                                                                           aTCTATTTTGGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACCAGATGGACCTATTATTGCTACTACTTcccc          <  1:310297/141‑1 (MQ=255)
                                                                                                                           aTCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACCAGATGGACCTATTATTGCTACTACTTcccc          <  1:393286/141‑1 (MQ=255)
                                                                                                                           aTCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACCAGATGGACCTATTATTGCTACTACTTcccc          <  1:653828/141‑1 (MQ=255)
                                                                                                                            tCTACTTGTGTCCCTTTCTCCGTTAATTCATTGCCCTCACAAACCACTTGAACTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACCAGATGGACCTATTATTGCTACTACTTCCCCt         <  1:53244/141‑1 (MQ=255)
                                                                                                                             cTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACCAGATGGACCTATTATTGCTCCTACTTCCGCtt        >  2:771050/1‑141 (MQ=255)
                                                                                                                                   gtgtCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACCAGATGGACCTATTATTGCTACTACTTCCCCttgattga  <  2:736214/141‑1 (MQ=255)
                                                                                                                                   gtgtCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACCAGATGGACCTATTATTGCTACTACTTCCCCttgattga  <  2:736198/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                     
ATGTAATTCATCGTTATTATCTTTCTTTAATAATTTAGGAGCTAAAATAATATTATCGACAACTTTTTTATGTGGAAATAGGTTGAAGTTTTGAAATACCATACCCATTTTTTGACGTAGTTTATCTACTTGTGTCCCTTTTTCCGTTAAGTCATTGCCTTCAAAAATCACTTGACCTTTAGTAGGTACTTCTAATAAATTCATACATCTTAACAATGTACTTTTACCACTACCAGATGGACCTATTATTGCTACTACTTCCCCTTGATTGA  >  CP000730/1990783‑1991054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: