Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,9300TA60.9% 91.4 / 163.1 139intergenic (‑1013/‑155)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/25);  new base A (49/35);  total (79/60)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 142 total aligned reads displayed.

GCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATTTTTCTTTT  >  CP000730/1929785‑1930076
                                                                                                                                                      |                                                                                                                                                         
gCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                                                                                                                       >  1:49257/1‑149 (MQ=255)
gCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                                                                                                                       >  1:183987/1‑149 (MQ=255)
gCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑C‑TGCTC‑TGGCGGTGCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                                                                                                                       >  1:261236/1‑149 (MQ=255)
 cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                                      >  1:62495/1‑149 (MQ=255)
 cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                                      >  1:754182/1‑149 (MQ=255)
 cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                                      >  1:132276/1‑149 (MQ=255)
  ttGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCTTTGCGTTTTTTTCTTAt                                                                                                                                                      >  1:981883/1‑149 (MQ=255)
  ttGTATATTCACAGATGGTATAATAACAGAGTCGCCTATCCCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                                                     <  1:355960/149‑1 (MQ=255)
   tgtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                                    >  1:895637/13‑149 (MQ=255)
   tgtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                                    >  1:602427/13‑149 (MQ=255)
   tGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAtt                                                                                                                                                     >  1:807893/1‑149 (MQ=255)
   tGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAtt                                                                                                                                                     >  1:486318/1‑149 (MQ=255)
   tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAttt                                                                                                                                                    >  1:506270/1‑148 (MQ=255)
    gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                                                                                                   <  1:896506/149‑1 (MQ=255)
     tatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                                                  >  1:839872/11‑149 (MQ=255)
     tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTTTTTTCGTTCACATCATAGCACCAGTCATCAGTGTATGTGCCATTGCGTTTTTTTCTTATTgg                                                                                                                                                   <  1:729361/149‑1 (MQ=255)
     tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTgg                                                                                                                                                   <  1:237891/149‑1 (MQ=255)
     tataTTCACAGATGGTATAATAAGAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                                                  <  1:386217/149‑1 (MQ=255)
      atattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                                                                                                 <  1:1114300/140‑1 (MQ=255)
       tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                                                                                                >  1:1085557/9‑148 (MQ=255)
          tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                                             >  1:992980/6‑149 (MQ=255)
          tcacagatggtATAATAATAGAGTCGCCTATCTCACAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGACCCAGACATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttcgt                                                                                                                                             >  1:487263/6‑145 (MQ=255)
           cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTAGACTTATTGGCTTAGTa                                                                                                                                            >  1:384838/5‑149 (MQ=255)
           cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                                            >  1:674639/5‑149 (MQ=255)
            gCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTa                                                                                                                                            >  1:322561/1‑149 (MQ=255)
             cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACACCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                                                                                          <  1:745835/147‑3 (MQ=255)
             cagatggtAAAATAATAGAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                                                                                          <  1:424423/147‑3 (MQ=255)
              cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGa                                                                                                                                          <  1:139008/149‑1 (MQ=255)
              agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTC‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc                                                                                                                                         <  1:990328/148‑1 (MQ=255)
               gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGGTCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                                                                                                        >  1:864963/1‑149 (MQ=255)
                atggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAAGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGATGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                                       >  1:771563/5‑149 (MQ=255)
                 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                                                                                                      <  1:153888/146‑1 (MQ=255)
                 tggtataATAACAGAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                                                                                                      <  1:303724/146‑1 (MQ=255)
                 tgggataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                                                                                                      <  1:3392/145‑1 (MQ=255)
                 gggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                                                                                                      <  1:591071/146‑1 (MQ=255)
                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑a                                                                                                                                   <  1:294907/147‑1 (MQ=255)
                   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGTCTTAGGAAACGCa‑‑ac                                                                                                                                  >  1:653214/2‑148 (MQ=255)
                   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGACATCAGTTGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑ac                                                                                                                                  >  1:866839/2‑148 (MQ=255)
                   gtataATAATAGAGTACCCTATCTCTCATGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGGTA‑ATCATTTTCGTTAACATCTGTGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑ac                                                                                                                                  <  1:541532/148‑2 (MQ=255)
                    tataATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑At                                                                                                                                  >  1:1030441/1‑149 (MQ=255)
                    tataATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGAA‑‑At                                                                                                                                  >  1:1030367/1‑149 (MQ=255)
                    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑aca                                                                                                                                 <  1:350166/149‑3 (MQ=255)
                    tataAGAGGAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑At                                                                                                                                  <  1:65477/149‑1 (MQ=255)
                     ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaa                                                                                                                                <  1:1035910/149‑4 (MQ=255)
                     ataataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATa                                                                                                                                 <  1:934251/149‑1 (MQ=255)
                      taataaCAGAGTCGCCGACCTCTACGGCGTTAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑G‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaa                                                                                                                               <  1:13349/149‑5 (MQ=255)
                        ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGATTAGTAAACGCa‑‑acaaata                                                                                                                             >  1:293289/5‑143 (MQ=255)
                        ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCGGTTGCGTATTATCCTTATTGCCTTAGTAAACGCa‑‑acaagaa                                                                                                                             >  1:882551/5‑143 (MQ=255)
                        ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaata                                                                                                                             <  1:796739/149‑7 (MQ=255)
                        ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaata                                                                                                                             <  1:848168/149‑7 (MQ=255)
                         tagtagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGAGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACaa‑‑a                                                                                                                           >  1:587387/1‑149 (MQ=255)
                         taatagCGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataa                                                                                                                            <  1:856797/146‑8 (MQ=255)
                         taatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCCGTCATCAGTGGCTGTGCTGTTGCGTAATATACTTATTGGCTTAGTAAACGCaccaaataa                                                                                                                              >  1:52079/4‑141 (MQ=255)
                           caattgTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                                          >  1:1056579/6‑140 (MQ=255)
                            tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTTGCTTAGTAAACGCa‑‑acaaatactta                                                                                                                         >  1:520622/1‑139 (MQ=255)
                            gagagGCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATa                                                                                                                        <  1:183159/148‑1 (MQ=255)
                            aagagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatta                                                                                                                         <  1:1050621/148‑11 (MQ=255)
                             agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGCGGAGGTGCA‑TTACT‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                                        >  1:286889/1‑138 (MQ=255)
                             agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataagtaa                                                                                                                        >  1:178157/1‑138 (MQ=255)
                             agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCCGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                                        >  1:90655/1‑138 (MQ=255)
                             agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                                        >  1:827910/1‑138 (MQ=255)
                              gagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTTGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAgg                                                                                                                      >  1:677342/1‑149 (MQ=255)
                              gagTCGCCTATCGCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTGC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGaa‑‑acaaataattaat                                                                                                                       >  1:801831/1‑137 (MQ=255)
                               tgTCGCCTATTTCTCAGGCGTCAATT‑‑GACGACGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCTTTCACATCATAGCACCAGTCATAAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                                      <  1:123551/148‑14 (MQ=255)
                               agTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGt                                                                                                                     >  1:200587/1‑149 (MQ=255)
                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTCTTATACTTATTGGCTTAGTAAACGCaccaaataattaata                                                                                                                        >  1:308666/1‑135 (MQ=255)
                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                                      >  1:122637/1‑136 (MQ=255)
                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                                      >  1:895275/1‑136 (MQ=255)
                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                                      >  1:112014/1‑136 (MQ=255)
                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                                      >  1:701008/1‑136 (MQ=255)
                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                                      >  1:853568/1‑136 (MQ=255)
                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑T‑TTTCGAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                                      <  1:429133/149‑14 (MQ=255)
                                gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTg                                                                                                                    >  1:477015/1‑149 (MQ=255)
                                gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑ATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCA‑‑ACACAT‑‑AAttaatag                                                                                                                   >  1:282303/1‑143 (MQ=255)
                                 gcGCCTATCTCTCCGGCGCCAATTTAGACGCAGAGAGGAGGCGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGGTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGa                                                                                                                   <  1:166657/148‑1 (MQ=255)
                                   gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACa                                                                                                                 <  1:242156/149‑1 (MQ=255)
                                             cAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATAATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGGTAAGTATACGCA‑‑ATACAA‑‑AATCGGTAGCT‑TAT‑AGCG‑GCa                                                                                                    >  1:244120/1‑149 (MQ=255)
                                                    cAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATCCAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATaa                                                                                             >  1:524404/1‑149 (MQ=255)
                                                              gCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATGCAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTc                                                                                   >  1:274713/1‑149 (MQ=255)
                                                              gCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGGTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGGTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTc                                                                                   >  1:65542/1‑149 (MQ=255)
                                                                  agagGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCATGGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCTATAAAAATCCCCTCACTa                                                                               <  1:814817/149‑1 (MQ=255)
                                                                                 a‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGAGAAA‑TAT‑TGCC‑GCACCAGTAAAAATCCCCTCACTACCGCCAATAGTGAg                                                                 >  1:586463/1‑149 (MQ=255)
                                                                                     g‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCCCTACCGCAAATAGTGgggg                                                               >  1:10834/1‑149 (MQ=255)
                                                                                             cTTATTTTCGTTCACATCATAGCACCCGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCC‑‑ATGCAT‑‑GGGAGGTGACA‑TAT‑AGCC‑GCACCCATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTgg                                                         <  1:214472/149‑1 (MQ=255)
                                                                                                      gTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGt                                                >  1:1074633/1‑149 (MQ=255)
                                                                                                       ttCACATCATAGCACCAGTCATCAGTGGCTGTTCCATTGCGTTTTTATCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTa                                               >  1:721197/1‑149 (MQ=255)
                                                                                                       ttCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTa                                               >  1:899438/1‑149 (MQ=255)
                                                                                                        tCACATCATAGCACCAGTCATCAGTGGCTGTGCCAATGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTaa                                              <  1:854300/149‑1 (MQ=255)
                                                                                                         cacaTCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTaaa                                             >  1:42761/1‑149 (MQ=255)
                                                                                                          acaTCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCCATAAAAATCCCCTCACTACCGCAAATAGTGTGGAGAATGGTGTATAAGTAAAt                                            >  1:432377/1‑149 (MQ=255)
                                                                                                                tAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAACAAATAATTAATAGGCGACTTTATAAGCCAGCATCGA‑AAAAATCCCCACACTACTGC‑AATAGTGTGGGGATTGGTGcattaagtgtta                                           >  1:975997/1‑140 (MQ=255)
                                                                                                                tAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAt                                      <  1:550275/149‑1 (MQ=255)
                                                                                                                     ccAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCg                                 >  1:936024/1‑149 (MQ=255)
                                                                                                                         tcatcaGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTGTTTTCGTTGt                             >  1:421890/1‑149 (MQ=255)
                                                                                                                               gTGGCTGTGCCATTGGTTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAAt                       <  1:87447/149‑1 (MQ=255)
                                                                                                                               gTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAAt                       <  1:648345/149‑1 (MQ=255)
                                                                                                                                       gCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGc               >  1:373903/1‑149 (MQ=255)
                                                                                                                                          cgtGCGTTTTTTTCTTAGTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTaa            <  1:478425/147‑1 (MQ=255)
                                                                                                                                                 ttttttCTTATTGGATAAGTCGACGCC‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATTTTTCt     <  1:357039/149‑1 (MQ=255)
                                                                                                                                                    tttCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAAGCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATTTTTCtttt  >  1:509531/1‑149 (MQ=255)
                                                                                                                                                      |                                                                                                                                                         
GCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑CTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATTTTTCTTTT  >  CP000730/1929785‑1930076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑