BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	36,378	C→T	L58L (TTG→TTA)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,378	0	C	T	90.5%	1296.0 / 124.2	664	L58L (TTG→TTA)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (33/29); new base T (290/302); total (331/333)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01

Only 100 of 666 total aligned reads displayed.

GTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCT  >  CP000730/36230‑36520

      tACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTTTACGTCTCATCAATATGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAtt                                                                                                                                          >  1:254078/1‑149 (MQ=11)

      tACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAtt                                                                                                                                          <  1:945933/149‑1 (MQ=255)

       aCGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTg                                                                                                                                         <  1:1087488/149‑1 (MQ=255)

        cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATAGTCGGCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                        <  1:155483/149‑1 (MQ=11)

        cGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAATCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                        <  1:267348/149‑1 (MQ=255)

        cGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                        <  1:97701/149‑1 (MQ=37)

         gCAACCAATTATCTAATGTATGTCTCTCTGTATTAATGGCACGATATAATCAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTATTCCAAATTTGATATAAAATTggg                                                                                                                                       <  1:564466/149‑1 (MQ=11)

           aaCCAAATATCTAATGTATGTCCCTCTGTAACAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGc                                                                                                                                     <  1:41386/149‑1 (MQ=255)

            aCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATTCGCCATTTGTAACAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTggtcgc                                                                                                                                    >  1:343760/1‑145 (MQ=255)

              cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGATCCATTTTCCTTTTATTTTGATATATGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTGTTTCTTCTACATTTGATATAGAATTGGGGCata                                                                                                                                  >  1:233207/1‑149 (MQ=11)

                aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTGCTTTTTCTTCCAAATTTGGTATAAAATTGGGGCATAtt                                                                                                                                >  1:883301/1‑149 (MQ=11)

                  tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCt                                                                                                                              <  1:504819/149‑1 (MQ=37)

                      tAATGTATGTCTCTTCGTATGAATGGCAAGATATAAATAGCTGCATTTTCCTTTTATTTCGATATACGTCTCATCAATACGGCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa                                                                                                                          <  1:511158/149‑1 (MQ=11)

                        atgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCGAATTTGATATAAAATTTGGGCTTATTCTTGAAcc                                                                                                                        >  1:956415/1‑149 (MQ=11)

                           tatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGCTATACGTCTCATCAATACGCCCTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAATTTTCATATAAAATTGCGGCATGTTCTTGAACCaaa                                                                                                                     >  1:934977/1‑149 (MQ=11)

                            atgtCCCTCTGTATCAATTGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc                                                                                                                    >  1:982160/1‑149 (MQ=255)

                            atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc                                                                                                                    <  1:201920/149‑1 (MQ=255)

                              gtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGTTCTTTCTTCCCAATTTGATATAAAATTGGGGCGTATTCTTGAACCCAACgg                                                                                                                  >  1:609945/1‑149 (MQ=11)

                              gtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTCGGGCATATTCTTGAACCCAACgg                                                                                                                  <  1:835191/149‑1 (MQ=255)

                              gtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTGTGCTTCCAAATTTGATATAAAATGGGGGCATATTCTTGGAccctccgg                                                                                                                  >  1:386014/1‑144 (MQ=11)

                               tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATAAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                                                                                                 >  1:902990/1‑149 (MQ=255)

                                cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTTTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCTTATTCTTGAACCCAACGGTa                                                                                                                >  1:633553/1‑149 (MQ=11)

                                cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCTAAAATTGGGGCATTATCTTGTACCCCACGGTa                                                                                                                >  1:440485/1‑149 (MQ=11)

                                cccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATTAATACGCCGTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTa                                                                                                                >  1:708440/1‑149 (MQ=255)

                                cccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTGGAACCCAACGGTa                                                                                                                >  1:843566/1‑149 (MQ=25)

                                  ctctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                                                                                              <  1:554961/149‑1 (MQ=18)

                                   tctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATGGGGGCATATTCTTGAACCCAACgggtgat                                                                                                             >  1:59272/1‑144 (MQ=11)

                                    ctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGatc                                                                                                            <  1:100039/149‑3 (MQ=255)

                                     tGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCg                                                                                                           <  1:1106256/149‑1 (MQ=18)

                                      gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                                                                                          <  1:285166/147‑1 (MQ=255)

                                      gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                                                                                          <  1:336585/147‑1 (MQ=255)

                                       taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGtt                                                                                                         <  1:19317/148‑1 (MQ=255)

                                         tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                       >  1:1082435/1‑149 (MQ=255)

                                         tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGATTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                       <  1:728097/149‑1 (MQ=11)

                                          cAATGGAACCATCTAAATAGCTCCATTTTCCGTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGaa                                                                                                      <  1:693495/149‑1 (MQ=255)

                                           aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTACGTCTCATTAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAat                                                                                                     <  1:784488/149‑1 (MQ=255)

                                            aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                    <  1:148131/149‑1 (MQ=255)

                                             tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatga                                                                                                   <  1:1061713/149‑1 (MQ=18)

                                               gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGTATATTCTTAAAACCAACGGTAGATCGTTGAatgata                                                                                                 >  1:138608/1‑148 (MQ=11)

                                                cgcGATATAAATATCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                                <  1:863445/147‑1 (MQ=11)

                                                cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                                <  1:164262/149‑1 (MQ=255)

                                                 aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGTCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                                                                                               >  1:309538/1‑149 (MQ=255)

                                                    atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGt                                                                                            >  1:181392/1‑149 (MQ=255)

                                                     tataAATAGCTCCATTTTCCTTTTCTCTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGtt                                                                                           <  1:921116/149‑1 (MQ=255)

                                                       taAACAGCTCCATTTTTCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                                                                                         <  1:775893/149‑1 (MQ=11)

                                                        aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAATTGCGGTCAGATTCTTAAACCCCACGGGAGATCGTTGAATGATGAACGTTTac                                                                                        >  1:1094179/1‑149 (MQ=11)

                                                        aaaTAGCTCCATTTTCCTTTTATTTTAATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAATGGTAGATCGTTGAATGATGAACGTTTac                                                                                        <  1:682426/149‑1 (MQ=11)

                                                           tAGCTTCTTTTGCGTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCAAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                     <  1:404260/149‑1 (MQ=11)

                                                            aGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATGTTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                                    >  1:380271/1‑149 (MQ=25)

                                                             gCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                                   >  1:321704/1‑149 (MQ=255)

                                                             gCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                                   >  1:700099/1‑149 (MQ=255)

                                                             gCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                                   <  1:107476/149‑1 (MQ=255)

                                                              cTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                                  >  1:471690/1‑149 (MQ=255)

                                                               tCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAACGATGAACGTTTACACCACGt                                                                                 >  1:830111/1‑149 (MQ=21)

                                                               tCCATTTTCCCTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAGATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                                 >  1:649757/1‑149 (MQ=255)

                                                                  aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAGTAAGCGTTTTTATGCTTGTTCTTCCAAATTTGATATAAAATTGGGTCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTct                                                                              <  1:985825/149‑2 (MQ=11)

                                                                  aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTct                                                                              <  1:1008157/149‑2 (MQ=255)

                                                                   ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctc                                                                             <  1:428171/149‑3 (MQ=255)

                                                                   ttttCCTTTTATTTTGATATACGTCCCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctc                                                                             <  1:758114/149‑3 (MQ=11)

                                                                     ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                           >  1:458931/1‑149 (MQ=40)

                                                                      tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                          <  1:123018/149‑1 (MQ=11)

                                                                          tttatttgtgtatACGTCTCATCAATACGCCATGTGTAATAAGCTTTTGTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                      <  1:372175/137‑1 (MQ=11)

                                                                          tttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                      >  1:523781/1‑149 (MQ=21)

                                                                          tttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                      >  1:916669/1‑149 (MQ=255)

                                                                          tttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                      >  1:393610/1‑149 (MQ=11)

                                                                           ttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAtt                                                                     <  1:574459/149‑1 (MQ=255)

                                                                            tATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCATCGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAttt                                                                    <  1:767726/149‑1 (MQ=11)

                                                                             aTTTTTATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGAATTTTACAGTTATTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTc                                                                   <  1:536866/149‑1 (MQ=11)

                                                                             aTTTAGATATACGTCTCATCAATACGCCAATTCTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTCCTCTTAATATTTc                                                                   <  1:35292/149‑1 (MQ=11)

                                                                               tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAg                                                                 >  1:664148/1‑149 (MQ=21)

                                                                               tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAg                                                                 <  1:225584/149‑1 (MQ=40)

                                                                                 tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                               <  1:444545/149‑1 (MQ=255)

                                                                                 tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                               >  1:484566/1‑149 (MQ=255)

                                                                                  gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGata                                                              >  1:643096/5‑149 (MQ=255)

                                                                                      tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                          >  1:736181/1‑149 (MQ=25)

                                                                                      tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                          <  1:1119982/149‑1 (MQ=21)

                                                                                       aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                         >  1:1037585/1‑149 (MQ=255)

                                                                                       aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                         >  1:790371/1‑149 (MQ=255)

                                                                                             catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                   >  1:548331/1‑148 (MQ=255)

                                                                                             catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGATCCCTTAATATTTCAAATATATCACTATaa                                                   >  1:133966/1‑149 (MQ=255)

                                                                                               tcaATACGCCATTTGTCATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                 <  1:1025579/149‑1 (MQ=255)

                                                                                               taaaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                 <  1:365671/147‑1 (MQ=11)

                                                                                                 aaTACGCCATTTGTAATAAGCTTTTTTAGGATTTTTCTTCCAAGTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGCTGAATTATGAACGTTTACACCACGTTCTCTTAATATTTCTGATATATCACGATAGCTCa                                               <  1:249227/149‑1 (MQ=11)

                                                                                                   tACGCCATTTGTAATAAGCTTTTTTATGCTTTTCCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGCATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                             <  1:1055371/149‑1 (MQ=255)

                                                                                                   aaCGCCATTTGTAATAAGCTTTTTTATGCTTCTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                             <  1:60009/148‑1 (MQ=21)

                                                                                                    aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAtt                                            >  1:853362/1‑148 (MQ=255)

                                                                                                    aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                            <  1:750130/149‑1 (MQ=39)

                                                                                                    aCGCCATTTGTAATAAGCTTTTTTATGCTTTTACTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGACATATCGCGATAACGCAATg                                            >  1:716653/1‑149 (MQ=11)

                                                                                                      gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCCGGCTTGTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                          <  1:816417/149‑3 (MQ=11)

                                                                                                      gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                          >  1:20876/1‑147 (MQ=11)

                                                                                                       ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATGcat                                         <  1:1041902/149‑1 (MQ=11)

                                                                                                       ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgcat                                         >  1:2954/1‑146 (MQ=17)

                                                                                                       ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAGTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCGTTAATATTTCAGATATATCACGATAACTCAATGcat                                         >  1:871761/1‑149 (MQ=255)

                                                                                                        cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATGcata                                        <  1:500371/149‑1 (MQ=11)

                                                                                                           ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGCATATCt                                     >  1:971605/1‑149 (MQ=17)

                                                                                                           ttGTAATAAGCTTTTGTATGCTTTTTCTTCCAAATTGGATAGAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTGCAGATATATCACGATAGCTCAATGCATATCt                                     <  1:272631/149‑1 (MQ=11)

                                                                                                            tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGCTATAAAATTGGGGCATATTCGTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGCATATCtt                                    <  1:260638/149‑1 (MQ=255)

                                                                                                             gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGCATATCTTa                                   >  1:570788/1‑149 (MQ=9)

                                                                                                              taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAGGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATGTCACGATAGCTCAATGCATATCTTAg                                  >  1:689192/1‑149 (MQ=11)

                                                                                                              taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGCATATCTTAg                                  <  1:895361/149‑1 (MQ=2)

GTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCT  >  CP000730/36230‑36520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`