Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 1,646,189 G→C A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,646,1890GC94.9% 113.0 / NA 40A155G (GCA→GGA) pepPM24B family Xaa‑Pro dipeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (10/27);  minor base T (1/1);  total (12/28)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTTTTA  >  CP000730/1646044‑1646332
                                                                                                                                                 |                                                                                                                                               
ttCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtt                                                                                                                                              >  1:148214/1‑149 (MQ=255)
  cAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCATATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTGTTTCAGTCATGCCTCCtttt                                                                                                                                            <  1:586115/149‑1 (MQ=255)
  cAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtttt                                                                                                                                            <  1:936196/149‑1 (MQ=255)
   aataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                           <  1:99859/149‑1 (MQ=255)
   aataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                           <  1:724133/149‑1 (MQ=255)
   aataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                           <  1:796973/149‑1 (MQ=255)
       atTTTCTCACTTTCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                                       <  1:1091785/149‑1 (MQ=255)
          ttattcgttGCACCGCCACGTGGTAACGGACTTCTGTGACAGGAGGCTACAATCGTATAGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGt                                                                                                                                    <  1:428673/142‑1 (MQ=255)
          tgaTCACTTGCAACCCCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTACTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGt                                                                                                                                    <  1:678497/147‑1 (MQ=255)
                    cAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATTCCTTCTTTTACTACAATTAAAatatat                                                                                                                          >  1:669343/1‑149 (MQ=255)
                    cAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAACTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatatat                                                                                                                          <  1:996179/149‑1 (MQ=255)
                     aaCACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAtt                                                                                                                         >  1:410974/1‑149 (MQ=255)
                                   aTGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatca                                                                                                           <  1:230769/149‑1 (MQ=255)
                                           ttATGACCAGATGCTACTATCGTATCGAATGATGCTCTATGCTCTCTGAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATAtt                                                                                                   <  1:585488/148‑1 (MQ=255)
                                                   aGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTTATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtt                                                                                           <  1:395472/149‑1 (MQ=255)
                                                       gCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTg                                                                                       <  1:747433/149‑1 (MQ=255)
                                                         caCAATCTTACCCAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGaa                                                                                     <  1:398390/148‑1 (MQ=255)
                                                         caCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGaa                                                                                     <  1:301889/148‑1 (MQ=255)
                                                             aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTaa                                                                                 <  1:916761/149‑1 (MQ=255)
                                                             aTCGTATCGAATGACGGTCCATCTGCTCCTAATTCTAGCACACTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTaa                                                                                 <  1:1546/149‑1 (MQ=255)
                                                                 tATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATTAACAATATTAGCTGTTTTTTGAATTAAAGCa                                                                             >  1:123801/1‑149 (MQ=255)
                                                                    cGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAtt                                                                          <  1:667298/149‑1 (MQ=255)
                                                                                        ccTAATTCTCGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATACCCATTGGCGGCATTACTAAAGATGGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtc                                                      <  1:266539/149‑1 (MQ=255)
                                                                                          tAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTACTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACCATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTAAGAATCTTTGACGtctc                                                    >  1:495179/1‑149 (MQ=255)
                                                                                              tCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAt                                                >  1:1040342/1‑149 (MQ=255)
                                                                                               cTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTGTTCAGTCATGCCTTCTTTTACAACAGTTAAAATCTATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtt                                               <  1:384386/149‑1 (MQ=255)
                                                                                                  gCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAttttt                                            >  1:530365/1‑148 (MQ=255)
                                                                                                     ttttGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCt                                         <  1:288701/149‑1 (MQ=255)
                                                                                                        tGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTAAAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTTAGCAGCCTTTACGTCTCTCATGTTATCTACa                                      >  1:86144/1‑149 (MQ=255)
                                                                                                          cTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTACTTTTATCTACAGt                                    >  1:609165/1‑149 (MQ=255)
                                                                                                           tttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTa                                   <  1:924324/149‑1 (MQ=255)
                                                                                                                    tttgcTTTAATTCTTTTTCATGCATGCCTCCTCGTACAACAGTTAAAATATATCCCTATGTTTCATCAACAACATTAGCGGCTTTTTGAATGAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATg                          <  1:369174/145‑1 (MQ=255)
                                                                                                                      gCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAACTTTATCTACAGTATTAGAAATGCt                        <  1:669303/149‑1 (MQ=255)
                                                                                                                       ccTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtt                       <  1:1052870/149‑1 (MQ=255)
                                                                                                                         tttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttat                     <  1:174653/149‑1 (MQ=255)
                                                                                                                          ttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACCGTATTAGAAATGCttatt                    >  1:972101/1‑149 (MQ=255)
                                                                                                                               tCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGa               >  1:665021/1‑149 (MQ=255)
                                                                                                                                       aGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAGTTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGc       >  1:377179/1‑149 (MQ=255)
                                                                                                                                            tGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTttta  <  1:1051172/149‑1 (MQ=255)
                                                                                                                                            tGCCTCCTTTTACAACAGTTAAAATATAGTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTTTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTttta  <  1:636673/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTTTTA  >  CP000730/1646044‑1646332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑