Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,224,814	T→C	I121V (ATT→GTT)	atpA ←	F‑ATPase superfamily proton(H+)‑ or sodium (Na+)‑translocating F‑type ATPase epsilon alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,224,814	0	T	C	100.0%	271.7 / NA	78	I121V (ATT→GTT)	atpA	F‑ATPase superfamily proton(H+)‑ or sodium (Na+)‑translocating F‑type ATPase epsilon alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/46);  total (32/46)

GATTAACTCACGTTGACCTCTACCAATTGGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAATCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCTGTGTATGGTCCTAAAATAACCAC  >  CP000730/2224674‑2224958                                                                                                                                             |                                                                                                                                                 tatTAACTCCCGTTGACCTCTACCAATTGGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCtt                                                                                                                                          <  1:972625/148‑1 (MQ=255)       cTCACGTTGACCTCTACCAATTGGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCAt                                                                                                                                    >  1:1008091/1‑149 (MQ=255)       cTCACCTTCACCTCTACCAATTTGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCAt                                                                                                                                    <  1:776402/149‑1 (MQ=255)         cACGTTGACCTCTACCAATTGGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCa                                                                                                                                  <  1:380101/149‑1 (MQ=255)         cACGTTGACCTCTACCAATTGGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCa                                                                                                                                  >  1:398017/1‑149 (MQ=255)         cACGTTGACCCCTACCAATTGGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCa                                                                                                                                  <  1:169954/149‑1 (MQ=255)         cACGGTTACCTCTACCAATTGGTACCACAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCa                                                                                                                                  <  1:245329/149‑1 (MQ=255)                aCCTCTACCAATTGGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGtt                                                                                                                           >  1:907540/1‑149 (MQ=255)                 ccTCTACCAATTGGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTg                                                                                                                          <  1:328529/149‑1 (MQ=255)                   tctACCAATTGGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTc                                                                                                                        <  1:170945/149‑1 (MQ=255)                       ccAATTGGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTaa                                                                                                                    <  1:493680/149‑1 (MQ=255)                       ccAATTGGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGGTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTaa                                                                                                                    >  1:1063253/1‑149 (MQ=255)                         aaTTGGTACTAAAGCATCCATTGCTTTTATACTTTTTTTGAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATg                                                                                                                  <  1:206805/149‑1 (MQ=255)                         aaTTGGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATg                                                                                                                  <  1:52773/149‑1 (MQ=255)                         aaTTGGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCGACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATg                                                                                                                  <  1:159751/149‑1 (MQ=255)                           ttGGTACTATAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGa                                                                                                                <  1:750311/149‑1 (MQ=255)                           ttGGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTCACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGa                                                                                                                >  1:550923/1‑149 (MQ=255)                           ttGGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGa                                                                                                                >  1:237367/1‑149 (MQ=255)                                 cTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTaaca                                                                                                          <  1:361709/149‑1 (MQ=255)                                 cTAAAGCATCAATTGCTATGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTaaca                                                                                                          >  1:964034/1‑149 (MQ=255)                                      gCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAActct                                                                                                     >  1:1046321/1‑149 (MQ=255)                                       cATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCtt                                                                                                    <  1:1065112/149‑1 (MQ=255)                                         tCAATTGCTTTGATACCTGTTTGTAATGGCTCTCCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTcc                                                                                                  <  1:1035164/149‑1 (MQ=255)                                          cAATTGCTTTGATACTTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGCTTATCCTAATGGATTAACAACTCTTCCg                                                                                                 <  1:790005/149‑1 (MQ=255)                                            aTTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCAAATGGATCAACCAATCTTCCGAt                                                                                               >  1:176004/1‑149 (MQ=255)                                                cTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGCTTAGt                                                                                           >  1:436227/1‑149 (MQ=255)                                                 tttGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGtt                                                                                          <  1:1032897/149‑1 (MQ=255)                                                       aCCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCAc                                                                                    <  1:535607/149‑1 (MQ=255)                                                        ccTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCAcc                                                                                   >  1:179256/1‑149 (MQ=255)                                                             ttGTAAGGGCTCATCTTCTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTg                                                                              <  1:160407/149‑1 (MQ=255)                                                                tAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTa                                                                           <  1:341874/149‑1 (MQ=255)                                                                  aTGGCCAATCTACTGTGTTACGATCCATTACACCAGTAGCTTTTCTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCGTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACt                                                                         <  1:258982/149‑1 (MQ=255)                                                                      cTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCa                                                                     >  1:521526/1‑149 (MQ=255)                                                                         aTCTACCGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTGTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGa                                                                  <  1:1120058/149‑1 (MQ=255)                                                                          tCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGAt                                                                 >  1:633663/1‑149 (MQ=255)                                                                          tCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGAt                                                                 <  1:168448/149‑1 (MQ=255)                                                                          tCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATAAACCACACTTCCGGTTAGTTCTTCACCTACTGGTACTTCCATGAt                                                                 >  1:864895/1‑149 (MQ=255)                                                                                  tttACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTTTCCATCAATCGCTCGTCGTACTGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTACCCACATACGAGCAg                                                         >  1:262088/1‑149 (MQ=255)                                                                                      cGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACg                                                     >  1:1066086/1‑149 (MQ=255)                                                                                       gATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTCCGt                                                    >  1:342955/1‑149 (MQ=255)                                                                                       gATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGt                                                    <  1:1029875/149‑1 (MQ=255)                                                                                       gATCCATTAAACCAGTAGCTTCGTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCCTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGt                                                    <  1:292878/149‑1 (MQ=255)                                                                                        ctCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGtt                                                   <  1:59968/148‑1 (MQ=255)                                                                                        acccATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGtt                                                   <  1:421244/147‑1 (MQ=255)                                                                                        aTCCCTTACACCAGTAGCTTTGTTCTCTTCTGGACGTGTTTTAGTTGTGTTACCCGAATTGATTGCGTCAATAGGTTGTCCTAATGGATTAACAACTCTTCCTATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGtt                                                   <  1:261017/149‑1 (MQ=255)                                                                                        aTCAATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGtt                                                   <  1:453352/149‑1 (MQ=255)                                                                                            aTTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTCCTTCCATGATACGACCAGTACGTTTAAc                                               >  1:694871/1‑149 (MQ=255)                                                                                               acacCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCCGCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTc                                            >  1:796119/1‑149 (MQ=255)                                                                                               acacCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTc                                            <  1:474864/149‑1 (MQ=255)                                                                                               acacCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTc                                            <  1:88812/149‑1 (MQ=255)                                                                                               acacCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTAATCCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTc                                            <  1:27507/149‑1 (MQ=255)                                                                                                cacCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCg                                           >  1:110134/1‑149 (MQ=255)                                                                                                 cccAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGt                                          <  1:975647/148‑1 (MQ=255)                                                                                                 acCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATAGGACCAGTAGGTTTAACTTCGt                                          >  1:692103/1‑149 (MQ=255)                                                                                                     gTAGCTTTTCTCTCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCAcc                                      <  1:505910/149‑1 (MQ=255)                                                                                                       aGCTTTTTTCGCTACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCtt                                    <  1:305034/149‑1 (MQ=255)                                                                                                        tcTTTTCTCTCTACTGGACGTGTTTTAGTTGTGTTACCCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTc                                   <  1:54796/148‑1 (MQ=255)                                                                                                           tgtttCTTTGCTGGACGTGTTGTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGt                                <  1:776684/147‑1 (MQ=255)                                                                                                                   tACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTGCGGTTAGTTCTTCACCTACTGGTACTTCCATGATACGACCACTCCGTTTAACTTCGTCACCTTCAGTAATACCtt                        >  1:990549/1‑148 (MQ=255)                                                                                                                   tACTGGACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCACTAATAACtt                        >  1:940257/1‑148 (MQ=255)                                                                                                                    cccggtcGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCtgt                       <  1:21155/143‑1 (MQ=255)                                                                                                                    aCTGGACGTGTTTTAGGTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCtgt                       <  1:8873/149‑1 (MQ=255)                                                                                                                      ttgACGTGTTTTAGTTGTGTGAACCGGTCCTTGTCCATCAATAGGTTGGCCTAATGGATTAACAACTCTTCCGATTCGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCTgtgt                     <  1:1075758/147‑1 (MQ=255)                                                                                                                       ggACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCTGTGTa                    >  1:498531/1‑149 (MQ=255)                                                                                                                        gACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCTTCACCTTCAGTAATACCTgtgtct                   >  1:478981/1‑147 (MQ=255)                                                                                                                        gACGTGTTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCTGTGTAt                   >  1:313169/1‑149 (MQ=255)                                                                                                                           gtgtTTTAGTTGTGTTAACCGGTCCTTGTCCATCCATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCTGTGTATGGt                <  1:851692/149‑1 (MQ=255)                                                                                                                           gtgtTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCCTGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCTGTGTATGGt                <  1:1055250/149‑1 (MQ=255)                                                                                                                           gtgtTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCTGTGTATGGt                <  1:517423/149‑1 (MQ=255)                                                                                                                             gtTTTAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCTGTGTATGGTcc              <  1:64190/149‑1 (MQ=255)                                                                                                                             gtTTTAGTTGTGTTAACCGGTCCGTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCTGTGTATGGTcc              <  1:330785/149‑1 (MQ=255)                                                                                                                               tttAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCTGGGTATGGTGCTa            >  1:194682/1‑149 (MQ=255)                                                                                                                                ttAGTTGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCTGTGTATGGTCCTaa           >  1:588986/1‑149 (MQ=255)                                                                                                                                    ttGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTCACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCTGTGTATGGTCCTAAAATa       >  1:721643/1‑149 (MQ=255)                                                                                                                                    ttGTGTTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCTGTGTATGGTCCTAAAATa       >  1:722860/1‑149 (MQ=255)                                                                                                                                    ttGTGTTAACCGGTCCTTGTCCATCAAGGGGTTGTGCTAATGGATTAACAACTCTTACGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCTGTGTGTGGTCCTCAAATa       >  1:604785/1‑149 (MQ=255)                                                                                                                                      gtgtTAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCTGTGTATGGTCCTAAAATAAc     <  1:802093/149‑1 (MQ=255)                                                                                                                                         ttAACCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGGTTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTAAGTTTCACTTCGTCACCTTCAGTAATTACTGTGTATGTTCCTGAAATAAcctc  >  1:136532/1‑147 (MQ=255)                                                                                                                                             |                                                                                                                                                 GATTAACTCACGTTGACCTCTACCAATTGGTACTAAAGCATCAATTGCTTTGATACCTGTTTGTAATGGCTCATCTACTGATTTACGATCCATTACACCAGTAGCTTTTTTCTCTACTGGACGTGTTTTAGTTGTGTTAATCGGTCCTTGTCCATCAATAGGTTGTCCTAATGGATTAACAACTCTTCCGATTAGTTCTTCACCTACTGGTACTTCCATGATACGACCAGTACGTTTAACTTCGTCACCTTCAGTAATACCTGTGTATGGTCCTAAAATAACCAC  >  CP000730/2224674‑2224958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑