Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	478,502	C→A	D342E (GAC→GAA)	ndhF →	NADH dehydrogenase (ubiquinone)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	478,502	0	C	A	98.5%	210.6 / NA	68	D342E (GAC→GAA)	ndhF	NADH dehydrogenase (ubiquinone)
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (36/31);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAAATGGTA  >  CP000730/478356‑478646                                                                                                                                                   |                                                                                                                                                 tttATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGc                                                                                                                                                >  1:423535/1‑149 (MQ=255)  ttATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCCATATTCCACAACAATCATCTGCCAACGAAGCt                                                                                                                                               >  1:14470/1‑149 (MQ=255)       ttAGTTCAATGTGCATTGGGTGTATATTGAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAACCAAGCATCTGCTAAAGAAGCTTATgg                                                                                                                                          >  1:684105/1‑149 (MQ=255)       ttAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATgg                                                                                                                                          >  1:128136/1‑149 (MQ=255)        tAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGc                                                                                                                                         <  1:1034757/149‑1 (MQ=255)            tCAATGTGCATTGGGTGTATATTCCGCAGAGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGa                                                                                                                                     >  1:578175/1‑149 (MQ=255)              aaTGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGAtt                                                                                                                                   >  1:918434/1‑149 (MQ=255)              aaTGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGGGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATggagggagt                                                                                                                                   >  1:34455/1‑142 (MQ=255)              aaTGCGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATCTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGTTAAAGAAGCTTATGGCTGGAtt                                                                                                                                   >  1:121011/1‑149 (MQ=255)               aTGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTg                                                                                                                                  >  1:74549/1‑149 (MQ=255)                tgtgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGt                                                                                                                                 <  1:767526/149‑1 (MQ=255)                    cATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAGGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATg                                                                                                                             >  1:648239/1‑149 (MQ=255)                           tgtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAGCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACgtg                                                                                                                      >  1:637634/1‑149 (MQ=255)                              atatTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTAt                                                                                                                   <  1:254148/149‑1 (MQ=255)                                 ttCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGTGACGTGTATTAg                                                                                                                >  1:220719/1‑149 (MQ=255)                                    agcagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCta                                                                                                             >  1:63207/1‑149 (MQ=255)                                    agcagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCta                                                                                                             >  1:1106080/1‑149 (MQ=255)                                    agcagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCta                                                                                                             <  1:458527/149‑1 (MQ=255)                                      cagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCtatt                                                                                                           <  1:332392/149‑1 (MQ=255)                                          gagtaggcATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCg                                                                                                       <  1:252765/142‑1 (MQ=255)                                          gATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGTACGTGTATTAGCTATTATCg                                                                                                       >  1:451985/1‑149 (MQ=255)                                          gATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCg                                                                                                       <  1:834551/149‑1 (MQ=255)                                           attattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATCTTGCAAAACAAGCATCTGCTAAAGAAGCGTATGGCTGGGTTGTCATGGGACGTGTATGAGCCATTATCGt                                                                                                      >  1:50698/1‑149 (MQ=255)                                               ttCATTTAATATTGCACGGTATTTTTAAAGCAACATGAGGTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCa                                                                                                  >  1:547245/1‑149 (MQ=255)                                                tCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGCCGTGGATTCGCCCTTATCGTGCCAt                                                                                                 >  1:992812/1‑149 (MQ=255)                                                tCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAt                                                                                                 >  1:953686/1‑149 (MQ=255)                                                 cATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGCTTCAATATTCCAAATCAAGCATCTGCTAAAGAAGCTTATTGCTGGATTGTCATGGGACGGGTATTAGCTATTATCGTGGCAtt                                                                                                >  1:541630/1‑149 (MQ=255)                                                   tttAATATTGCACGGTATTTTTAAAGTAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGTCGTGTATTAGCTATTATCGTGGCATTTc                                                                                              <  1:680040/149‑1 (MQ=255)                                                    ttAATATTGCACGGTATTTTTAGAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCt                                                                                             >  1:733617/1‑149 (MQ=255)                                                     tAATACTCCACGGTATTTTTAAACCAACATTATTCTTCCAACCAGTTTCTATCGTGAAGCGAGTCACTATACCAAAACAAGCCTCTGCTAAAGAACCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTa                                                                                            <  1:56969/149‑1 (MQ=255)                                                        tatTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAttt                                                                                         <  1:742408/149‑1 (MQ=255)                                                         atTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAtttt                                                                                        <  1:410895/149‑1 (MQ=255)                                                          ttGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGATTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTg                                                                                       >  1:215660/1‑149 (MQ=255)                                                              aCGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCTTGGGACGTGTTTTAGCTATTATCGTGGTATTTCTATTTTGGGTg                                                                                   >  1:55116/1‑149 (MQ=255)                                                              aCGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATg                                                                                   >  1:642879/1‑149 (MQ=255)                                                              aCGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATg                                                                                   <  1:468589/149‑1 (MQ=255)                                                              aCGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATg                                                                                   >  1:162485/1‑149 (MQ=255)                                                                  tATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGagta                                                                               <  1:335393/149‑1 (MQ=255)                                                                  tATTTTCAAAGCAACATTAGTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGGTTGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGagta                                                                               <  1:495372/149‑1 (MQ=255)                                                                     ttttAAAGCAACATTATTTTTCAAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTg                                                                            <  1:103335/149‑1 (MQ=255)                                                                     ttttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTg                                                                            <  1:369865/149‑1 (MQ=255)                                                                      tttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGCCCTGGGTCGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGGAGTgc                                                                           >  1:313678/1‑148 (MQ=255)                                                                        tAAAGCAACATTATTTTTACAATAAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACa                                                                         >  1:832837/1‑149 (MQ=255)                                                                         aaaGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGTTGGAGTGTCATGGTACGTGTCTTAGCTATTATCGTGGAATTTCTATTTTGAATGAGAACTGACAg                                                                        >  1:538467/1‑149 (MQ=255)                                                                            gCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCCTTTCTATTTTGGATGAGTACTGACAGAAg                                                                     >  1:931233/1‑149 (MQ=255)                                                                            gCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAg                                                                     <  1:987901/149‑1 (MQ=255)                                                                               aCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGCTTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGGGACAGAAGTGc                                                                  >  1:67904/1‑149 (MQ=255)                                                                               aCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGc                                                                  >  1:381194/1‑149 (MQ=255)                                                                               aCATTATTTTTACAACCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGc                                                                  <  1:14700/149‑1 (MQ=255)                                                                               aCATGATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGc                                                                  <  1:896660/149‑1 (MQ=255)                                                                                cATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCa                                                                 <  1:415437/149‑1 (MQ=255)                                                                                       tttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAg                                                          >  1:549931/1‑149 (MQ=255)                                                                                       tttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGAGTTTGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGGATTTCTATTTTGGATGAGTAGTGACAGAGGTGCATGTGAAg                                                          >  1:652121/1‑149 (MQ=255)                                                                                        ttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATTAAgt                                                         >  1:1031522/1‑149 (MQ=255)                                                                                        ttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAgt                                                         <  1:793997/149‑1 (MQ=255)                                                                                               cAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGt                                                  >  1:582277/1‑149 (MQ=255)                                                                                                          ctGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCt                                       <  1:879818/148‑1 (MQ=255)                                                                                                                       atatTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTa                          <  1:945251/149‑1 (MQ=255)                                                                                                                          ttCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACtt                       <  1:799797/149‑1 (MQ=255)                                                                                                                           tCCAACACAAGCATCTGCTAAAGAAGCTTATGGCTGGAGTGTCATGGGACGTTTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTg                      <  1:1048553/149‑1 (MQ=255)                                                                                                                           tCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTg                      <  1:707924/149‑1 (MQ=255)                                                                                                                            ccAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGt                     >  1:597453/1‑149 (MQ=255)                                                                                                                                  cAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTg               <  1:848307/149‑1 (MQ=255)                                                                                                                                   acgCACCTTCTAAAGAAGCTCACGGCGTGATTGTCATGGGCCGTCTATTAGCTATTATCCTGGCATTTCTATTTTGGATGAGTAGTGACATAAGTGCATATGAAGTGTTAAGTGCACTCACTCTAGGATGGTCATTACTTGTATCTTgg              <  1:98758/147‑1 (MQ=255)                                                                                                                                   aaGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTgg              <  1:380338/149‑1 (MQ=255)                                                                                                                                   aaGCATCGGCTAAAGAAGGTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACGCATTCTAGTATGGTCATTACTTGTATCTTgg              <  1:734348/149‑1 (MQ=255)                                                                                                                                      cATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTAGTATCGTGGCATTTCTATTGTGGGTGCGCCGTCACATAGGGGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAAt           <  1:265565/149‑1 (MQ=255)                                                                                                                                          tGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGGATCaaa       >  1:573170/1‑149 (MQ=255)                                                                                                                                               aagaagCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAAATGGTa  >  1:251086/1‑149 (MQ=255)                                                                                                                                               aagaagCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAAATGGTa  >  1:1032463/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                 TTTATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAAATGGTA  >  CP000730/478356‑478646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑