Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,105,994	T→C	T12T (ACT→ACC)	pdhD →	dihydrolipoyl dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,105,994	0	T	C	100.0%	133.4 / NA	39	T12T (ACT→ACC)	pdhD	dihydrolipoyl dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/17);  total (22/17)

TAAGCTTTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATTACTACATG  >  CP000730/1105852‑1106139                                                                                                                                               |                                                                                                                                                   tAAGCTTTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTa                                                                                                                                              >  1:702171/1‑149 (MQ=255)        tggccATAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAg                                                                                                                                       <  1:941137/146‑1 (MQ=255)        tGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATCGTAATCgggg                                                                                                                                       >  1:999761/1‑147 (MQ=255)        tGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAg                                                                                                                                       <  1:462849/149‑1 (MQ=255)          aCCACATACAAATTGATGGTGCAACTGGCCAACATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑Ca                                                                                                                                    <  1:443027/149‑1 (MQ=255)           ccACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAg                                                                                                                                   >  1:344706/1‑149 (MQ=255)                      ttGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGCG‑CAGGTCCTGGTGGa                                                                                                                        >  1:307150/1‑149 (MQ=255)                            gTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGtt                                                                                                                  <  1:348646/149‑1 (MQ=255)                             tGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATACAAATTTAGTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTg                                                                                                                 >  1:771593/1‑149 (MQ=255)                               cAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTgca                                                                                                               >  1:252137/1‑149 (MQ=255)                                       cAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCg                                                                                                       <  1:782876/149‑1 (MQ=255)                                          aaTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGc                                                                                                    >  1:474947/1‑149 (MQ=255)                                           aTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCa                                                                                                   >  1:134043/1‑149 (MQ=255)                                            tGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAg                                                                                                  <  1:603965/149‑1 (MQ=255)                                              gaaTGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCt                                                                                                <  1:499761/148‑1 (MQ=255)                                              cAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCt                                                                                                <  1:1022478/149‑1 (MQ=255)                                                   aaTCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTACTCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCCATTCGTGCAGCTCAAtt                                                                                           >  1:210142/1‑149 (MQ=255)                                                     tCACATTAAACGTTTATTAAATAATCCAGAATTATTATTACTGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAg                                                                                         >  1:767977/1‑149 (MQ=255)                                                                      taAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATc                                                                        >  1:1057138/1‑149 (MQ=255)                                                                                          ttaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGGTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGGACTCTtgg                                                    >  1:178496/1‑149 (MQ=255)                                                                                               ggAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGgtggtg                                               <  1:978765/149‑1 (MQ=255)                                                                                                gAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCCATTAATTCATCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGgtgt                                              >  1:1098334/1‑149 (MQ=255)                                                                                                gAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGgtgt                                              >  1:20581/1‑149 (MQ=255)                                                                                                  ggggTAAAACATGGTAGTTGGAGATTTCCGAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGttt                                            >  1:587954/1‑149 (MQ=255)                                                                                                  ggggTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGttt                                            >  1:230919/1‑149 (MQ=255)                                                                                                    ggTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGc                                          <  1:1059643/149‑1 (MQ=255)                                                                                                     gTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCt                                         <  1:35659/149‑1 (MQ=255)                                                                                                              ggTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAg                                >  1:655997/1‑149 (MQ=255)                                                                                                               gTAGTTTGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAgg                               <  1:1026167/149‑1 (MQ=255)                                                                                                               gTAGTTGGAGATTTCCCAATGGAAACAGATCCCATAGTAATCGGAG‑CAGGCCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAgg                               <  1:515181/149‑1 (MQ=255)                                                                                                                tAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGa                              >  1:1095749/1‑149 (MQ=255)                                                                                                                 aGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGAt                             <  1:187903/149‑1 (MQ=255)                                                                                                                            tCCCAATTGAAACAGATACCATAGTACTAGGAGTCAGG‑CCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGGTGTATTCCTTCa                  >  1:547552/1‑149 (MQ=255)                                                                                                                              ccgaTTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCaaa                <  1:418356/146‑1 (MQ=255)                                                                                                                              ccAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCaaa                >  1:827489/1‑149 (MQ=255)                                                                                                                                 agttaaaCAGATACCAAAGGAAACGGAG‑CAGGGCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGc             <  1:176760/145‑1 (MQ=255)                                                                                                                                  ttGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCa            >  1:882841/1‑149 (MQ=255)                                                                                                                                         aGATACCATAGTAATCGGAG‑CACGTCCAGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTACTTAAACGTAGGATGTATTCCTTCAAAAGCATtactac     >  1:732454/1‑149 (MQ=255)                                                                                                                                            tACCATAGTTATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATTACTACATg  <  1:21577/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                   TAAGCTTTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATTACTACATG  >  CP000730/1105852‑1106139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑