Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,196,508	C→A	intergenic (+2563/‑1229)	ftsQ → / → ylmE	cell division protein FtsQ/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,196,508	0	C	A	100.0%	127.4 / NA	41	intergenic (+2563/‑1229)	ftsQ/ylmE	cell division protein FtsQ/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/17);  total (24/17)

CGTTAATATGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGACGTTCAA  >  CP000730/1196362‑1196651                                                                                                                                                   |                                                                                                                                                cGTTAATATGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGATCAAGCGTAAATACTtata                                                                                                                                               <  1:812908/149‑1 (MQ=255)       tatGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACCACCCAATAGCGCATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATg                                                                                                                                         <  1:491516/149‑1 (MQ=255)       tatGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCaagg                                                                                                                                         >  1:632806/1‑147 (MQ=255)       tatGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGAACTGGATTCGGAACAAGCGTAAATACTTATAGCAATg                                                                                                                                         >  1:96242/1‑149 (MQ=255)             tttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACtt                                                                                                                                   <  1:527771/149‑1 (MQ=255)              ttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACtta                                                                                                                                  >  1:80705/1‑148 (MQ=255)                 ggTACAGTTATTAATCCTGACTTACAAGATGAGATTGTTGTAAAAGTTATTGCAACTGGTTTTGAGGACAACCCACCATCACATGGTCGTAAATCTGGTAGCAGTGGATTCGGAACAAGCGTAAATACTTATAGCAACCGAACTTCTaa                                                                                                                               >  1:162879/1‑149 (MQ=255)                   tACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAg                                                                                                                             <  1:984851/149‑1 (MQ=255)                       gTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATACGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGa                                                                                                                         >  1:209545/1‑149 (MQ=255)                        ttattaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGaa                                                                                                                        >  1:620354/1‑149 (MQ=255)                             aaTCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAGTACTTATAGCAATGCAACTTCTAAAGATGAATCAtt                                                                                                                   >  1:998755/1‑149 (MQ=255)                              aTCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTc                                                                                                                  <  1:331300/149‑1 (MQ=255)                              aTCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTc                                                                                                                  >  1:621935/1‑149 (MQ=255)                                ccTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCAc                                                                                                                >  1:348046/1‑149 (MQ=255)                                  tGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACtt                                                                                                              <  1:690379/149‑1 (MQ=255)                                    aaTTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTGGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCa                                                                                                            >  1:862766/1‑149 (MQ=255)                                    aaTTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCa                                                                                                            >  1:635264/1‑149 (MQ=255)                                                agaTTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAAt                                                                                                >  1:109574/1‑149 (MQ=255)                                                      ttgtAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACaa                                                                                          >  1:951058/1‑149 (MQ=255)                                                            cAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAACTACTTATAGCAATGCAACTTCTAAAGATGAATCATTCCCTTCAAATTCCTCCAATGCACAAGCaaat                                                                                    >  1:846652/1‑147 (MQ=255)                                                            cAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACt                                                                                    >  1:846651/1‑149 (MQ=255)                                                                tactGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATa                                                                                <  1:710582/146‑1 (MQ=255)                                                                   ggCAACGGGTTTTGATGACAAACCAACAGCACATGGGCGGAAGGCGGGGAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTGCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAgtg                                                                             <  1:761853/148‑1 (MQ=255)                                                                     cAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTa                                                                           <  1:423947/149‑1 (MQ=255)                                                                     aaaCGGGTTTTGATGACAAACCAACAACACATGGTCGTAAATCTGGTAGCCCTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCAATTCACCGATATCAAATGCACAAGCAACTGATAGTGTa                                                                           <  1:271624/148‑1 (MQ=255)                                                                          ggTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCGTCAAATGCACAAGCAACTGATAGTGTAAGTGa                                                                      <  1:716876/149‑1 (MQ=255)                                                                           ggtttGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGaa                                                                     <  1:807897/147‑1 (MQ=255)                                                                            ttttGATGACAAACCAACATCACCAGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAGGTGaaa                                                                    >  1:223924/1‑149 (MQ=255)                                                                            ttttGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAACGCACAAGCAACTGATAGTGTAAGTGaaa                                                                    <  1:683915/149‑1 (MQ=255)                                                                              ttGATGAAAAACCAACATCACATGGTCGTAAATCCGGTAGCACCGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGa                                                                  <  1:279359/149‑1 (MQ=255)                                                                                       aaCCAACATCACATGGTCGTAACACTGGTAGCTGGGGATTCGGCCGGTCCTGGTGTACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACa                                                         <  1:811369/149‑1 (MQ=255)                                                                                               tCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAaaga                                                 <  1:354453/149‑1 (MQ=255)                                                                                                     ggTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACCTACAACTAAAGAAgatga                                           <  1:812896/149‑1 (MQ=255)                                                                                                     ggTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAgatga                                           >  1:625728/1‑149 (MQ=255)                                                                                                          tAAATCTGGTAGCACTGGATTCGGAACAAGCGTAACTTCTGATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTc                                      >  1:1107885/1‑149 (MQ=255)                                                                                                                           gATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAAt                     >  1:290232/1‑149 (MQ=255)                                                                                                                              tCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATaga                  >  1:217834/1‑149 (MQ=255)                                                                                                                                   aCAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCGAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTGGAAATAGagaaga             >  1:622636/1‑149 (MQ=255)                                                                                                                                        cGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAACTTCATCCCATGCACAGGAAAGTGATAATGTAAGTAAAAGAACACATACAACGAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGAc        <  1:224249/149‑1 (MQ=255)                                                                                                                                             aTACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGACGTTCa   >  1:74313/1‑149 (MQ=255)                                                                                                                                              tACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAGGTAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGACGTGCaa  >  1:31697/1‑149 (MQ=255)                                                                                                                                              tACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAATACGTTCaa  >  1:506890/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                CGTTAATATGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGACGTTCAA  >  CP000730/1196362‑1196651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑