BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,315	G→A	57.0%	Y79Y (TAC→TAT)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,315	0	G	A	57.0%	63.1 / 310.2	284	Y79Y (TAC→TAT)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (72/48); new base A (79/80); total (155/129)
Fisher's exact test for biased strand distribution p-value = 9.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

Only 100 of 286 total aligned reads displayed.

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTC  >  CP000730/36175‑36455

ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATg                                                                                                                                              <  1:15518/141‑1 (MQ=32)

     aaaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCGTTAGCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTACGt                                                                                                                                         >  1:329041/1‑141 (MQ=11)

      aaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGtc                                                                                                                                        <  2:157139/141‑1 (MQ=32)

        ttGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTCCGtctt                                                                                                                                      >  1:101219/1‑140 (MQ=17)

          ttttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCGTTTATTTTGATATACGTCtcat                                                                                                                                    <  2:23312/140‑1 (MQ=255)

           tttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcatc                                                                                                                                   <  2:367537/141‑1 (MQ=12)

           tttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcatc                                                                                                                                   >  2:173371/1‑141 (MQ=17)

             tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTTTTTTGATGTGCGTCTCATCaa                                                                                                                                 >  1:139883/1‑141 (MQ=12)

              aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATATATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                                                                <  1:127802/141‑1 (MQ=17)

              aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                                                                <  1:265336/141‑1 (MQ=32)

              aaTGAGACGTTTAATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                                                                <  1:304549/141‑1 (MQ=12)

               aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGCTATAAATAGCTCCATTTTCCTTTTATTTTGATCTTCGTCTCATCtata                                                                                                                               >  2:13127/1‑141 (MQ=11)

                tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATAc                                                                                                                              <  1:19791/141‑1 (MQ=32)

                  ggaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGc                                                                                                                            <  1:230225/140‑1 (MQ=17)

                  agaCGTTTGATAAACGCATATGCTGACTGATTATGTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAACGGCACTATATAAATAGCTCCATTTTCCTTTTATTTTGGTGTACGTCTCATCAATACGc                                                                                                                            <  1:226303/141‑1 (MQ=11)

                   gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTACCCTCAATAcccc                                                                                                                           >  2:38257/1‑141 (MQ=11)

                    aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCCACCAAATATCAAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCGCCATTTTCCTTTTATTTTGATGTACGTCTCCTCAACACGcaa                                                                                                                          >  2:37336/1‑139 (MQ=11)

                    aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCa                                                                                                                          <  1:25143/141‑1 (MQ=17)

                      gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCATTGGCACGATATAAATAGCTCTATTTTCCTTTTATTTTGTTATACGTCTCATGAATTAGCCagt                                                                                                                        >  1:41242/1‑139 (MQ=11)

                         tGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATTGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTTATCAATACTCCTTCTGt                                                                                                                     >  1:66066/1‑141 (MQ=11)

                         tGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGCTATACGCTTCATCAATACGCCCTTTga                                                                                                                     >  1:260914/1‑140 (MQ=11)

                         tGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGt                                                                                                                     <  1:351991/141‑1 (MQ=12)

                            tAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGGTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCTTTTGtaat                                                                                                                  >  2:104879/1‑141 (MQ=17)

                                cGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGc                                                                                                              <  2:73501/141‑1 (MQ=35)

                                    tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtttt                                                                                                          <  2:340904/141‑1 (MQ=25)

                                     atGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCttttt                                                                                                         <  2:305335/141‑1 (MQ=255)

                                      tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTACTAAGCtttttt                                                                                                        >  2:52506/1‑141 (MQ=255)

                                      tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCACTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGCTTTACGTCTCATCAATACGCCATTTGTAATAAGCtctctt                                                                                                        >  1:87294/1‑136 (MQ=255)

                                       gCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTa                                                                                                       <  1:52506/141‑1 (MQ=255)

                                            aTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTGGTAATAAGCTTATTTATGCtt                                                                                                  >  1:322340/1‑141 (MQ=255)

                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                             <  2:331660/141‑1 (MQ=39)

                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                             <  1:246105/141‑1 (MQ=40)

                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                             >  1:207254/1‑141 (MQ=40)

                                                  aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATATCTCCATTTTCCTTTTATTTTGATATACGTCTTATCAATACGCCATTTGTAGTAAGCTTTTTTATGAtttttttt                                                                                            >  1:119287/1‑141 (MQ=14)

                                                   tctcGTTTCTTAGCCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTCTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                           <  2:318953/141‑1 (MQ=14)

                                                    ctcGTTGCTTACGCAACCAGATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAAGAAGCTTTTTTATGCTTTTTCTTcc                                                                                          <  1:237443/141‑1 (MQ=255)

                                                    ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                                                                          <  2:199260/141‑1 (MQ=255)

                                                    cgcGTTGTTTACGCTACCAACTATCTCATGGATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                                                                          <  2:294979/139‑1 (MQ=14)

                                                     tcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGAGATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTAGTAATAAACTTTTTTATGATTTGTTTTCCa                                                                                         >  2:271527/1‑141 (MQ=14)

                                                         tGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACTTCTCATCAATACGCCATTTTTAATAAAGTTTTTTATGCTTTTTCTTCCAAAtt                                                                                     >  1:179564/1‑141 (MQ=11)

                                                         tGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAtt                                                                                     <  2:299310/141‑1 (MQ=40)

                                                             tACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAt                                                                                 <  2:336595/141‑1 (MQ=40)

                                                                gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATACATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                                                              <  2:260955/141‑3 (MQ=255)

                                                                gCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                                                              <  1:98140/141‑3 (MQ=38)

                                                                gCAACCAAATATCTAATGTATGTCCCTCTGCATAAATGGCACGATATAAAAAGCTCCATTTTCCTTTTATTTTGATGTACGTATCATTAGTACGCCATTTGTAATAAGCTTTTTTTTGCTTTTTCTTTCCAAtttcgtata                                                                              >  2:246750/1‑135 (MQ=14)

                                                                 cAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCTCAATTTGATATaa                                                                             >  2:315023/1‑141 (MQ=255)

                                                                   aCCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCTAATTTGATATaaaa                                                                           >  2:127530/1‑141 (MQ=255)

                                                                      aaaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTACTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTg                                                                        <  2:216872/141‑1 (MQ=255)

                                                                       aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTACCAAATTTGATATAAAATTgg                                                                       >  1:201462/1‑141 (MQ=255)

                                                                          atCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGc                                                                    <  1:344917/141‑1 (MQ=255)

                                                                          atCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTAATATAAAATTGGGGc                                                                    <  1:276688/141‑1 (MQ=255)

                                                                            cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                  <  1:336716/141‑1 (MQ=255)

                                                                             tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTATTATAAAATTGGGTCata                                                                 >  2:30302/1‑141 (MQ=255)

                                                                               atgtatgtCCCTCTGTATCAATGGCACGCTATAAATAGCTCCATTTTCCTTTTATATTGATATACGTCTCATCAATACGCCAATTGTAATAAGCTTCTTTATGCTTTTTCTTCCAAATTTGTTATAAAATTGTGGAatcct                                                               >  2:235197/1‑138 (MQ=11)

                                                                               atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAACTTTGTAAAAAATTGGGGCATAtt                                                               >  1:247239/1‑141 (MQ=255)

                                                                               atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTATCATGAATAGGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAAAAAATTTGGTGATAtt                                                               >  2:132722/1‑141 (MQ=14)

                                                                                 gtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGGTATAAAATTGGGGCATATTCt                                                             >  1:286583/1‑141 (MQ=255)

                                                                                   atgtCCCTCTGCATCAATGGCACGATATCAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTGTGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                           <  1:210529/141‑1 (MQ=255)

                                                                                      tCCCTCTGTATCAATGGCACGATATCAACACCTTCAGTTTCATTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGATTTTTTATGCTTTTTCTTCCAAATTTGATAGAAAATTGGGGCGTTTTATTGAAc                                                        >  1:370948/1‑141 (MQ=11)

                                                                                      tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                        >  2:192946/1‑141 (MQ=255)

                                                                                          tctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGCTATAAAATTGGGGCATATTCTTGAACCCaa                                                    >  2:341770/1‑141 (MQ=21)

                                                                                             gCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACgg                                                 <  1:131510/141‑1 (MQ=40)

                                                                                             gCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTCTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTCATATAAAGTTGGGGCTTCTTATTGAGCCCAActg                                                 >  1:279851/1‑139 (MQ=11)

                                                                                                tCAATGGCACGATATAAATAGATCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                              >  2:106950/1‑141 (MQ=21)

                                                                                                 cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAgc                                             >  1:217888/1‑140 (MQ=21)

                                                                                                   aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATGTAAAATTGGGACATATACTTGAACCCAACGGTCGatc                                           >  2:51410/1‑139 (MQ=11)

                                                                                                   aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGagt                                           >  2:142606/1‑139 (MQ=255)

                                                                                                     ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTCATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCCACGGTCGACCGt                                         >  2:356164/1‑141 (MQ=255)

                                                                                                      gaaCGATATAAATAGCTCCATTTTCCTTTTATTTTAATGTACGTCTCACCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGtt                                        <  1:290660/139‑1 (MQ=255)

                                                                                                        aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                      >  1:61633/1‑141 (MQ=21)

                                                                                                         cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTTaa                                     >  2:209491/1‑141 (MQ=11)

                                                                                                         cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                     <  2:146819/141‑1 (MQ=255)

                                                                                                               aaaTAGCTCCATTTTCCCTTTCTTTTGATATCCGTCTCATCAATACACCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGAAATAAAATTGGGCCCAATTCTTGACCCCAACGTTAGACCGCTCAatgatca                               >  2:76587/1‑139 (MQ=11)

                                                                                                                    gCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                          >  1:189418/1‑141 (MQ=255)

                                                                                                                       ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTac                       <  1:108389/141‑1 (MQ=255)

                                                                                                                        cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTTATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTgca                      >  2:84621/1‑141 (MQ=255)

                                                                                                                        cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                      >  1:83311/1‑141 (MQ=255)

                                                                                                                        cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAAGTTTaca                      >  2:325511/1‑141 (MQ=255)

                                                                                                                          ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAACGATGAACGTTTAcacc                    <  2:61633/141‑1 (MQ=21)

                                                                                                                          ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                    >  1:67842/1‑141 (MQ=40)

                                                                                                                          ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTACTACGAATGTGGAGGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                    <  1:103640/141‑1 (MQ=11)

                                                                                                                          ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                    <  2:292805/141‑1 (MQ=255)

                                                                                                                          ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                    <  2:265812/141‑1 (MQ=255)

                                                                                                                           tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                   <  2:67842/141‑1 (MQ=40)

                                                                                                                           tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAAGTTTAcacca                   >  2:115596/1‑141 (MQ=255)

                                                                                                                            ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacaac                  >  2:11281/1‑141 (MQ=255)

                                                                                                                             tgattttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACACAACGGTAGATAGTTGAATGATGAACGTTTacacaaag                 >  2:371443/4‑137 (MQ=11)

                                                                                                                             tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg                 <  1:194960/141‑1 (MQ=21)

                                                                                                                             tCCTTTTATTTGGATATACGTCTTATAAGTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                 <  2:151392/141‑1 (MQ=11)

                                                                                                                              ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAAACCACGt                >  1:27019/1‑141 (MQ=255)

                                                                                                                               cTTTTATTTTGATGTACGTCCCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt               <  1:93491/141‑1 (MQ=255)

                                                                                                                                  ttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGTTctc            >  1:171932/1‑139 (MQ=11)

                                                                                                                                   tATTTTGATATACGTCTCCTCAATACGCCATTTGTAATAAGCTTTTTTAGGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctct           <  1:38186/141‑1 (MQ=11)

                                                                                                                                    tttttGGTATTCGGCTCACCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt          <  2:8837/140‑1 (MQ=11)

                                                                                                                                    tttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt          <  1:310540/140‑1 (MQ=39)

                                                                                                                                      tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGCACCCAACGGTAGACCGTTGAATGATGAACGTTTACACAACGTTCCCTTaa        >  2:255387/1‑141 (MQ=255)

                                                                                                                                      tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGACCCCAACGGTAGACCGTTGAATGATGAACGTTTACACAACGTTCCCTTaa        <  2:217888/141‑1 (MQ=255)

                                                                                                                                       ttGATGTACGTCTCATCAATACGCCATTCGTAATAAGCTTTTTTTTTTTTTTTTTTCCAAATTTGATATAAAATTGGGGCATATTTTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACTCTATTTTCCCTTAat       >  2:273653/1‑141 (MQ=11)

                                                                                                                                       ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAat       <  2:308586/141‑1 (MQ=255)

                                                                                                                                       ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAat       <  1:297539/141‑1 (MQ=11)

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTC  >  CP000730/36175‑36455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`