Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,265 C→T 34.7% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2650CT34.7% 189.2 / 191.0 220intergenic (+323/‑811)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (83/60);  new base T (43/33);  total (127/93)
Fisher's exact test for biased strand distribution p-value = 8.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 220 total aligned reads displayed.

CAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAG  >  CP000730/76129‑76403
                                                                                                                                        |                                                                                                                                           
 aatgaagtTAACAACGATAGAAAAAAAAAGGTTCTTTTGCAAAGTTGAATCTATAGTAAAAAAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTgg                                                                                                                                        <  2:196424/141‑1 (MQ=11)
          aataatTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCt                                                                                                                               >  2:335496/1‑141 (MQ=255)
                      gtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAtt                                                                                                                   <  2:237635/134‑1 (MQ=255)
                      gtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATAGAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAtt                                                                                                                   <  1:51004/134‑1 (MQ=11)
                       gttagaaGGTTCTTTTGCAAAGTTGAATTTATAATATAATTAAAAACAAAAGTAATCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                                                                                                                  <  2:145906/135‑1 (MQ=11)
                        aaTAGAGGTTCTGGTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGCGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                                                                                                                 <  2:262436/141‑1 (MQ=255)
                         aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                                                                                                                <  1:333272/137‑1 (MQ=2)
                           tgaGGTTCTGTTGCAAAGTTGCATTTATAGTATAATTTTAAACAATAGAAGAGTTCCGTACAAACTATTTCAGATATAAACATTTTAACAATGATTTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGtt                                                                                                              <  2:359269/139‑1 (MQ=11)
                           ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAAAAAGGAGGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTgcggt                                                                                                              >  1:134857/1‑137 (MQ=255)
                            gaGGTTCTGTTGCAAAGTTGAATTTATAATATAATCTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgctc                                                                                                             >  2:66066/2‑138 (MQ=255)
                             aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATTTTGGGCATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                                                                                                            >  1:271527/1‑141 (MQ=1)
                                        gaaaGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTTTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATc                                                                                                 <  2:112078/140‑1 (MQ=255)
                                        cAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc                                                                                                 >  1:128590/1‑141 (MQ=35)
                                            agtGAATCTATAGTAAAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTGGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaa                                                                                             <  1:290331/139‑1 (MQ=255)
                                               tgttCTGTAGTATAATTTTAACAAAAAGGAGGCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTGTCACTGTAGCCGTTGGCTACTATCTAAGATATGCGTTGAGTTATCGTGATATATCTGAAata                                                                                          >  2:3970/4‑141 (MQ=11)
                                                         tataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGCTTTAAACAATTTAACAAGGATGTTCAACCGGGAGCCGTTGCCCTCAATTTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAAc                                                                                <  1:87723/141‑1 (MQ=11)
                                                             aTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtgg                                                                           <  1:117299/141‑1 (MQ=12)
                                                              ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACatggt                                                                          >  1:206246/1‑141 (MQ=12)
                                                              ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGAACAAAAAAACTAAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggt                                                                          <  1:121091/141‑1 (MQ=11)
                                                                ttAACAAAAAGGGGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGgtgt                                                                        <  2:107521/141‑1 (MQ=12)
                                                                ttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                                                        <  1:310058/141‑1 (MQ=17)
                                                                tcaaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACCGTAGCTGTTGGCTACCATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                                                        <  1:191513/139‑1 (MQ=255)
                                                                    cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAAc                                                                    >  2:28773/1‑141 (MQ=17)
                                                                    aaaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAAc                                                                    <  2:284906/140‑1 (MQ=12)
                                                                     aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACg                                                                   <  2:132959/141‑1 (MQ=17)
                                                                     aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACg                                                                   <  1:255967/141‑1 (MQ=12)
                                                                     aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACg                                                                   <  1:160928/141‑1 (MQ=17)
                                                                       aaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGtt                                                                 >  1:73529/1‑141 (MQ=17)
                                                                         aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtca                                                               >  1:260038/1‑141 (MQ=17)
                                                                         aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtca                                                               <  2:49281/141‑1 (MQ=17)
                                                                           gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGTTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATAACTGAAATATTAAGAGGACGTGGTGTAGACGTtcatc                                                             >  2:188712/1‑141 (MQ=11)
                                                                           gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatc                                                             >  2:210508/1‑141 (MQ=17)
                                                                           gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTTAGATAACGTGGTGTAAACGTtcatc                                                             <  2:264711/141‑1 (MQ=255)
                                                                            aGTCTTCTGTATGAACTATTTCAGATATAATCAGTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatca                                                            >  2:343205/1‑141 (MQ=12)
                                                                            aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatca                                                            >  1:370462/1‑141 (MQ=32)
                                                                             gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGGCTGTGCGAGAGAACTGAAATATTAAGGGAACGTGGTGTAAACGTTcatcat                                                           >  1:84621/1‑141 (MQ=11)
                                                                             gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTcatcat                                                           >  1:130731/1‑141 (MQ=17)
                                                                             gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTcatcat                                                           >  1:171190/1‑141 (MQ=12)
                                                                             gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGCTcatcat                                                           >  2:200650/1‑141 (MQ=12)
                                                                             gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGCTGTAAACGTTcatcat                                                           >  2:268382/1‑141 (MQ=12)
                                                                             gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTACCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACATTcatcat                                                           >  2:260914/1‑141 (MQ=255)
                                                                              tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCAtt                                                          <  2:47172/141‑1 (MQ=17)
                                                                              tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTAATCAtt                                                          <  2:90476/141‑1 (MQ=17)
                                                                               cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTc                                                         >  2:101219/1‑141 (MQ=17)
                                                                               cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTc                                                         <  1:109016/141‑1 (MQ=17)
                                                                                ttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAGACGTTCATCATTCa                                                        >  2:364287/1‑141 (MQ=12)
                                                                                    gTATGAATTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACga                                                    <  1:28499/141‑2 (MQ=12)
                                                                                    gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACgg                                                    <  2:22444/141‑1 (MQ=32)
                                                                                      aTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACgatc                                                  <  1:51947/141‑4 (MQ=255)
                                                                                       tgcaCTATTTCAGATATAAACAAGTTAACAAGGATGTTAGCACTTTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCt                                                 <  2:12903/138‑1 (MQ=11)
                                                                                       tGAACTATTTCAGATCTAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTACGATATGCATTGAGGTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAAAGTTCATCATTCAACGGTCt                                                 >  2:213949/1‑141 (MQ=11)
                                                                                       tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCt                                                 <  2:136146/141‑1 (MQ=255)
                                                                                          aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAcc                                              <  1:97905/141‑1 (MQ=17)
                                                                                          aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                              >  1:60671/1‑141 (MQ=12)
                                                                                          aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                              >  1:213637/1‑141 (MQ=12)
                                                                                           cTATTTCAGCTATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATCTGCATTGAGTTATCGTGATATGACTGAATGATTAGGGCGACGTGGTGTTGACGTTCCTCATTGCACGGTCTTCCg                                             >  2:292637/1‑141 (MQ=11)
                                                                                            tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGt                                            <  2:272632/141‑1 (MQ=32)
                                                                                             aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                           >  1:356484/1‑141 (MQ=17)
                                                                                                tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                        <  1:291983/141‑1 (MQ=12)
                                                                                                 cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATGATTCAAGGATCTACCGTTGGGt                                       >  2:197192/1‑141 (MQ=11)
                                                                                                  aGATCTAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTCCATCATTCAACGATCTACCGTTGGGtt                                      <  1:194941/141‑1 (MQ=11)
                                                                                                  aGATATAAACAATTTACCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                      >  2:171932/1‑141 (MQ=11)
                                                                                                  aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                      <  1:116124/141‑1 (MQ=255)
                                                                                                   gATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAACTATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTc                                     >  2:165617/1‑141 (MQ=17)
                                                                                                   gATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTc                                     <  1:234082/141‑1 (MQ=255)
                                                                                                   gATATAAACAATTTAACAAGGATGTTATCACTGCAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCAGAAATAGTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTc                                     >  2:246389/1‑141 (MQ=11)
                                                                                                       taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                 >  1:66343/1‑141 (MQ=17)
                                                                                                       taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                 <  2:305998/141‑1 (MQ=17)
                                                                                                       taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                 >  1:146869/1‑141 (MQ=17)
                                                                                                       taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                 <  2:159781/141‑1 (MQ=17)
                                                                                                         aaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAat                               <  2:54938/141‑1 (MQ=32)
                                                                                                            aaTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATg                            <  1:137732/141‑1 (MQ=17)
                                                                                                              tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACTATCTACCGTTGGGTTCAAGAATATGcc                          <  2:3467/141‑1 (MQ=255)
                                                                                                               ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGccc                         >  2:83311/1‑141 (MQ=255)
                                                                                                                 aaCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCa                       >  1:281408/1‑141 (MQ=17)
                                                                                                                 aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTAATCCTTCAACGGTCTACCGTTGGGTTCAAGAATGTGCCCCa                       >  2:8626/1‑141 (MQ=12)
                                                                                                                  aCAAGGGTGTTCTCACTGTAGCGGTTGGCTACTATCTAAGTTATGCATGGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                      >  2:370948/1‑141 (MQ=11)
                                                                                                                  aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAAAATTAAGGGAACGTGGTGTAAACGTTCAGCATTAAACGGTCTACGGTTGGTGTCAAGATTATGCCCCaa                      >  2:72824/1‑141 (MQ=11)
                                                                                                                  aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATGTTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                      >  1:198167/1‑141 (MQ=255)
                                                                                                                  aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTGCAAGACTATGCCCCaa                      >  1:327803/1‑141 (MQ=11)
                                                                                                                  aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAAAATTAAGAGACCGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAACATGCCCCaa                      >  2:326591/1‑141 (MQ=11)
                                                                                                                   cAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGTGCTATCATAATATAACTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAt                     >  1:113394/1‑141 (MQ=11)
                                                                                                                    aaGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATGTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTATACCGTTGGGTTCAAGAATATGCCCCAAtt                    >  2:27694/1‑141 (MQ=12)
                                                                                                                    aaGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtt                    >  2:280789/1‑141 (MQ=255)
                                                                                                                    aaGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtt                    >  1:173371/1‑141 (MQ=12)
                                                                                                                     aGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGAGCAGTGCAGGAAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCACttt                   >  1:367510/1‑141 (MQ=11)
                                                                                                                        aTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                <  2:233478/141‑1 (MQ=255)
                                                                                                                         tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata               <  2:217926/141‑1 (MQ=255)
                                                                                                                          gTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACATTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCACCAATTTTatat              >  1:216961/1‑141 (MQ=255)
                                                                                                                           ttATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc             >  1:336915/1‑141 (MQ=25)
                                                                                                                           ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc             >  2:193814/1‑141 (MQ=255)
                                                                                                                            tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa            <  1:297940/141‑1 (MQ=255)
                                                                                                                             aTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGGTCAAGAATATGCCCCAATTTTATATCaa           >  1:94327/1‑141 (MQ=21)
                                                                                                                                  tGTAGCCGTTGGTTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg      <  1:303295/141‑1 (MQ=21)
                                                                                                                                   gcaGCCGTTGGCTACTATCTAAGACATGCATTGAGTTATCGCGATATATCTGAAATATTAAGGGAACGTGGTGTAATCGCTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg     <  1:82767/139‑1 (MQ=255)
                                                                                                                                   gaaGCCGTTGGCTACTATCCAAGATATGGATTGAGTTATCGTGATATATCTGAAAGATCACGGGAACGGGGTGTACACGTTCATCCCTCAACGGTCTACCGCTGGGCCCAAGAATATGCCCCAATTTTATATCAAATTTgg     <  2:104167/139‑1 (MQ=11)
                                                                                                                                   gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg     >  2:115989/1‑141 (MQ=35)
                                                                                                                                    tAGCCGTTGGCTACTATCTAAGATCTGCATTGAGCTATCGTGATATATCGGAAGTATTAAGAGACGGTGGAGAAAAGGTTCATCATTCAACGATCGAGCGTTGGGTGCAAGAATATGCCCCAAGTTTAGATCAAATTTGga    >  2:320178/1‑141 (MQ=11)
                                                                                                                                    tAGCCGTTGGCTACTAGCTAAGATATGCATTGAGCTATTGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATACAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga    >  1:207752/1‑141 (MQ=11)
                                                                                                                                      gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag  >  1:245417/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                           
CAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAG  >  CP000730/76129‑76403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: