Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 707,909 A→T 100% M1M (TTG→ATG) † mntB ← ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730707,9090AT96.4% 79.5 / ‑4.4 27M1M (TTG→ATG) †mntBABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (11/15);  total (12/15)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGA  >  CP000730/707782‑708041
                                                                                                                                |                                                                                                                                    
gAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATAcctc                                                                                                                         >  1:38279/1‑141 (MQ=255)
  aGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGAAAATGTTTTTAAGTACATGCTTATTACCTAAAAACGGATTTAAATCTTTTGTTTCTAACATAACGTTATCcctcct                                                                                                                       >  2:8019/1‑141 (MQ=255)
                 aTTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAgg                                                                                                        >  1:73972/1‑141 (MQ=255)
                      ggCCCATGATACCAATTATTTCGCCGCGTTCTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAAc                                                                                                   <  1:159397/141‑1 (MQ=255)
                       gCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                                                  <  2:93759/141‑1 (MQ=255)
                                    attattTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTATCATCAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGtat                                                                                     <  1:368016/141‑1 (MQ=255)
                                          tCGCCGCTTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATaaa                                                                               <  2:2215/141‑1 (MQ=255)
                                             ccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTCGAGACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTg                                                                            <  1:194700/141‑1 (MQ=255)
                                             ccgcATAATAGTATCGATAAAGAAATGTTTTTAAGTACATGCATATAACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTg                                                                            <  2:88569/141‑1 (MQ=255)
                                                    cTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAatat                                                                     <  1:15307/141‑1 (MQ=255)
                                                        tATCTATAAGTAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTGTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTAt                                                                 <  1:356702/141‑1 (MQ=255)
                                                                 ggAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATATACTGAGAATATTTATCATGTCAAg                                                        <  1:272362/141‑1 (MQ=255)
                                                                  gAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTAATCTAGTCaaat                                                       >  2:200942/1‑139 (MQ=255)
                                                                   aaaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                                                      <  1:107402/141‑1 (MQ=255)
                                                                      tGTTTTTAAGTACATGCTTATTGAATAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAt                                                   >  2:361166/1‑141 (MQ=255)
                                                                              aGTACATCCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGata                                           <  1:174270/141‑1 (MQ=255)
                                                                                   aTGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATATCAAGTAAATTCGTGatataata                                      >  2:130679/1‑141 (MQ=255)
                                                                                           taCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTCAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAat                              <  2:189343/141‑1 (MQ=255)
                                                                                            aCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATCGTACACTTGAGTGTTAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACActg                             >  1:254364/1‑141 (MQ=255)
                                                                                               tAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgtat                          >  2:47536/1‑141 (MQ=255)
                                                                                                  aaaGAGATTTAAATCTTTTGTTTCTAACATACGTTTATTCCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATATCAAGTAAATTCGTAATATAATATAGACAATTTAtgtg                       >  2:274350/1‑141 (MQ=255)
                                                                                                     aaGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAgg                    <  1:326099/140‑1 (MQ=255)
                                                                                                        aTTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTATGATAACCTATTTAATTTTATAATAAACTGAGAATCTTTATCAGGTAAAGTAACTTCGTGATATAATATAGACAATGTATGTGAGTTGa                 >  2:279128/1‑141 (MQ=255)
                                                                                                            aaaTCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGt             <  2:112472/141‑1 (MQ=255)
                                                                                                                      tttCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTTAGTGGGGAGAAAGGATGTTAAcgg   >  1:42632/1‑139 (MQ=255)
                                                                                                                      tttCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGGGAAAGTATGTTAACTg   >  1:18600/1‑141 (MQ=255)
                                                                                                                       ttCTAACATTCGTTTATACCTCCTAATTAATAGTTTAGGCTAACCTAATTAATTGAATAAAAAACTGAGAATATTAATCCTGTCAAGTAAATTCGAGATATAAAATAGACAATGTATGTGAGGTGAAAGTATGTaaaaaaa  <  2:285480/141‑5 (MQ=255)
                                                                                                                                |                                                                                                                                    
GAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGA  >  CP000730/707782‑708041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: