Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 708,019 T→C 13.8% intergenic (‑110/‑12) mntB ← / → sirR ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730708,0190TC13.8% 54.3 / 3.1 29intergenic (‑110/‑12)mntB/sirRABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (13/12);  new base C (2/2);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

AAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGGCGATAAAAACTTTGTGACAAATAAAATCTTATCTCAATTTTTAAATATTAAGCCTCCATCTGTAAGTGAA  >  CP000730/707880‑708153
                                                                                                                                           |                                                                                                                                      
aaaGAGATTTAAATCTTTTGTTTCTAACATACGTTTATTCCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATATCAAGTAAATTCGTAATATAATATAGACAATTTAtgtg                                                                                                                                       >  2:274350/1‑141 (MQ=255)
   aaGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAgg                                                                                                                                    <  1:326099/140‑1 (MQ=255)
      aTTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTATGATAACCTATTTAATTTTATAATAAACTGAGAATCTTTATCAGGTAAAGTAACTTCGTGATATAATATAGACAATGTATGTGAGTTGa                                                                                                                                 >  2:279128/1‑141 (MQ=255)
          aaaTCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGt                                                                                                                             <  2:112472/141‑1 (MQ=255)
                    tttCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTTAGTGGGGAGAAAGGATGTTAAcgg                                                                                                                   >  1:42632/1‑139 (MQ=255)
                    tttCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGGGAAAGTATGTTAACTg                                                                                                                   >  1:18600/1‑141 (MQ=255)
                     ttCTAACATTCGTTTATACCTCCTAATTAATAGTTTAGGCTAACCTAATTAATTGAATAAAAAACTGAGAATATTAATCCTGTCAAGTAAATTCGAGATATAAAATAGACAATGTATGTGAGGTGAAAGTATGTaaaaaaa                                                                                                                  <  2:285480/141‑5 (MQ=255)
                                     taCCTCCTAATTAAGAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTAt                                                                                                  >  2:253831/1‑141 (MQ=255)
                                        cttctAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTa                                                                                               <  1:288461/138‑1 (MQ=255)
                                               ttAAAAGTTTAGGCTAACCAAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCaa                                                                                        <  2:209387/141‑1 (MQ=255)
                                                           gCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGaata                                                                            >  1:333492/1‑141 (MQ=255)
                                                           gCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGaata                                                                            >  1:333546/1‑141 (MQ=255)
                                                               aCCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATgg                                                                        >  2:355722/1‑141 (MQ=255)
                                                                   aattaattGTATAATAAACTGAGAATATTTATCGTGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGCCGAt                                                                    <  1:253831/141‑1 (MQ=255)
                                                                   aattaaatGTATAATAAACGAAGAATATTTAGCATGTCAAGTAAATTCGTGATAGAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGGCGAt                                                                    <  2:178653/141‑1 (MQ=255)
                                                                     ttaatgGAATAATAAACTGAGAATTCTTAGCATGTCAAGTAAATTCGTGATACAATATAGACACTGTCTGTGTGGTGAGAGTATGCTAGCTGAAGGAAAAGAGGACTATTTGACGGCAATCCTTACGAATCATGGCGATaa                                                                  <  2:234500/141‑1 (MQ=255)
                                                                              taataaACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAAACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCACTCCTTACGAATAATGGCGATAAAAACTTtgt                                                         >  1:16020/1‑141 (MQ=255)
                                                                                ataaACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAACACTATTGAAAGTCAATCCTTACGAATAATGGCGATAAAAACTTTGTGa                                                       >  1:121053/1‑141 (MQ=255)
                                                                                         gaATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGGCGATAAAAACTTTGTGACAAATaaaa                                              >  1:90730/1‑141 (MQ=255)
                                                                                            aatTTATCATGTCAAGTAAATTCGTGATAGAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGGCGATAAAAACTTTGTGACAAATAAAATCt                                           <  1:227597/140‑1 (MQ=255)
                                                                                              tttATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATATAAAGGCAATCCTTACGAATAATGGCGATAAAAACTTTGTGACAAATAAAATCtaa                                         >  2:132145/1‑139 (MQ=255)
                                                                                                 aTCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGCGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGGCGATAAAAACTTTGTGACAAAt                                                  >  1:13095/1‑129 (MQ=255)
                                                                                                 aTCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGCGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGGCGATAAAAACTTTGTGACAAAt                                                  <  2:13095/129‑1 (MQ=255)
                                                                                                 aTCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGCGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGGCGATAAAAACTTTGTGACAAAt                                                  >  1:196532/1‑129 (MQ=255)
                                                                                                 aTCATGCAAAGTAAATTCGTGATATAATATAGACAATGTATGCGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGGCGATAAAAACTTTGTGACAAAt                                                  <  2:196532/129‑1 (MQ=255)
                                                                                                          aGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGGCGATAAAAACTTTGTGACAAATAAAATCTTATCTCAATTTTTa                             <  1:47536/141‑1 (MQ=255)
                                                                                                                   gTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGGCGATAAAAACTTTGTGACAAATAAAATCTTATCTCAATTTTTAAATATTAAg                    >  2:24364/1‑141 (MQ=255)
                                                                                                                             ataGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATGTAAAGGCAATCCTTACGAATAATGGCGATAAAAACTTTGTGACAAATAAAATCTTATCTCAATTTTTAAATATTAAGCCTCCATcgg          >  2:79097/1‑139 (MQ=255)
                                                                                                                              taGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGGCGATAAAAACTTTGTGACAAATAAAATCTTATCTCAATTTTTAAATATTAAGCCTCCATCTGt         <  2:333492/141‑1 (MQ=255)
                                                                                                                              taGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGGCGATAAAAACTTTGTGACAAATAAAATCTTATCTCAATTTTTAAATATTAAGCCTCCATCTGt         <  2:333546/141‑1 (MQ=255)
                                                                                                                                  caacctatgtGAGGTGAAAGTATGTTATCTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGGCGATAAAATCTTTGTGACAAATAAAATCTTATCTCAATTTTTAAATATTAAGCCTCCATCTGTAAGt     <  1:200942/136‑1 (MQ=255)
                                                                                                                                     tgtatgtGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGGCGATAAAAACTTTGTGACAAATAAAATCTTATCTCAATTTTTAAATATTAAGCCTCCATCTGTAAGTGaa  >  1:282926/1‑141 (MQ=255)
                                                                                                                                     tgtatgtGAGGGGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGGCGATAGAAACTTTGTGACAAATAAAATCTTATCTCAATTTTTAAATATTAAGCCTCCATCCGTAAGTGaa  >  1:78553/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                      
AAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAGAGGACTATTTAAAGGCAATCCTTACGAATAATGGCGATAAAAACTTTGTGACAAATAAAATCTTATCTCAATTTTTAAATATTAAGCCTCCATCTGTAAGTGAA  >  CP000730/707880‑708153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: