BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,940	G→A	34.5%	R204R (CGC→CGT)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,940	0	G	A	34.5%	181.4 / 156.7	181	R204R (CGC→CGT)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (47/71); new base A (31/31); total (78/103)
Fisher's exact test for biased strand distribution p-value = 2.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

Only 100 of 183 total aligned reads displayed.

TGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35799‑36080

tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                                              <  2:213429/136‑1 (MQ=11)

tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                                              <  1:81528/136‑1 (MQ=11)

 attttaataaAAAT‑GATGGTTCTGTTGCAAAG‑AAAAAAATATAGCTAATCACTAATATATCATGTCAGTGGACGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAACCGTAGATATGAAGAGACCTGCg                                                                                                                                             >  2:365966/5‑141 (MQ=11)

  ttttaataaAAAT‑GCTGGTTCTGTTGCAAAGT‑AAAAAATATAGCCAACCACTAATATATCCTGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTGCGTTGCATGGCGAAAATCCGGAGATCTGAAGAGACCTGCgg                                                                                                                                            <  1:321997/138‑1 (MQ=11)

  aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                                              <  2:10051/141‑1 (MQ=255)

   tCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                                                                                             <  1:213002/141‑1 (MQ=255)

    ttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                                                                                          <  2:340933/140‑1 (MQ=11)

     taagaaAAATTGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATCTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                                                                                           <  2:61688/141‑1 (MQ=255)

       ataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                                                                                                         <  2:156609/141‑1 (MQ=38)

                                                                    cAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                           <  1:75650/141‑1 (MQ=11)

                                                                      gttttCGTTTTACTTGCTAGCATGATGCTAATTTCGAGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                         <  1:220734/138‑1 (MQ=11)

                                                                      gtgtTCGTTGAAGTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                         <  2:241365/141‑1 (MQ=11)

                                                                      gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                         >  1:320397/1‑141 (MQ=32)

                                                                       tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                                                        >  1:249908/1‑141 (MQ=255)

                                                                       tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                        >  2:325923/1‑141 (MQ=17)

                                                                        gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                                                       >  2:340462/1‑141 (MQ=12)

                                                                         ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAACATCCGCAGGTGTGAAAAGGCCTGCGGTTCTTATTATATCAGA‑CACCAAAACACTCCATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                      >  2:113727/1‑141 (MQ=11)

                                                                         ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTCTGCAGAGACCTGCGGTTCTTTTTGTAT‑AGAGCGTAACTACACTCAATACCTTTTAAAGTATTATTTTCTGTATGTATACTTTgg                                                                      >  2:240568/1‑140 (MQ=11)

                                                                          tCGTTTAACTTGCTTGCATGATGCTAATTTCGTGGCATGGCGAAATTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                     <  2:370183/141‑1 (MQ=255)

                                                                           cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATa                                                                    >  2:139994/4‑141 (MQ=255)

                                                                           cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                                                    <  1:193857/138‑1 (MQ=17)

                                                                            gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCCATGCCTGTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                   >  1:287963/1‑141 (MQ=255)

                                                                            gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                   <  2:49348/141‑1 (MQ=17)

                                                                            gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                   <  2:365020/141‑1 (MQ=17)

                                                                             tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                  <  2:351589/140‑1 (MQ=17)

                                                                             cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGTCCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                  <  2:229755/141‑1 (MQ=17)

                                                                              ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAGCCCTGAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatct                                                                 <  1:288064/141‑4 (MQ=11)

                                                                               tAACTTGCCAGCATGTTGCTAATTTCGTGGCATGTGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑ATCGCGTAAATACGTTCAATACCTTTTAACGTATTCTTTGCCTTATTGATACTTTGATATCtt                                                                <  2:164765/141‑1 (MQ=11)

                                                                                aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATTCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGCAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTg                                                               <  1:122546/141‑1 (MQ=11)

                                                                                aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTg                                                               >  2:183620/1‑141 (MQ=255)

                                                                                  cTTGCTAGCATGATGCTAATGTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                             <  1:67282/141‑1 (MQ=17)

                                                                                   ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                            <  2:320869/141‑1 (MQ=17)

                                                                                    tGCTAGCATGCTGCTCCTCTAGTGGAATGGCGCTAATCCGTTGCTCTGCAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                           <  1:77379/141‑1 (MQ=11)

                                                                                    tGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctt                                                           >  1:6175/1‑141 (MQ=255)

                                                                                    tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                           <  1:333939/141‑1 (MQ=17)

                                                                                     gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                          <  2:106598/141‑1 (MQ=17)

                                                                                     gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                          <  2:262409/141‑1 (MQ=17)

                                                                                     gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACATTGtcttt                                                          >  1:10176/1‑141 (MQ=17)

                                                                                      cTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtatttc                                                         >  2:247793/1‑141 (MQ=11)

                                                                                       tAGCATGATGCTAATTTCGTGGCATGAGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATAGATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                                                        <  1:227971/141‑1 (MQ=11)

                                                                                        aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttactt                                                       >  2:250530/1‑141 (MQ=17)

                                                                                         gCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGGTACTTGGATATCTTGtctttctgt                                                      >  1:27449/1‑139 (MQ=11)

                                                                                            tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                   >  1:354009/1‑141 (MQ=255)

                                                                                            tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                   >  1:374363/1‑141 (MQ=11)

                                                                                             gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATATCAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                  >  2:32939/1‑141 (MQ=255)

                                                                                              atgCTAATTTCGTGGCATGGAGAAAATCCGTAGATCTGAAGAGAGCTACGGTTCTTTTGATAT‑AGAGCGTAAATACATTCAATACCTTTTAAGGTATTCTTTGCCGTATTGATCATTTGATATCTCGTCTTTCTTACTTTa                                                 >  1:226146/1‑141 (MQ=11)

                                                                                               tgCTAATTTCGTGGCATGGCGCAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATTT‑AGAGCGGAAATACACCCAATACCTTTTAAAGTATTCTTTGCTGTTTTGATACTTTGATACCTTGTCTTTCTTACTTTaa                                                >  1:131354/1‑141 (MQ=11)

                                                                                                gCTAATTTCGTGGCATGGCGAAAATCAGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTatt                                               >  2:230869/1‑139 (MQ=19)

                                                                                                 cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                                              >  2:269327/1‑141 (MQ=21)

                                                                                                  tAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCGTACTTTAatat                                             >  1:232312/1‑141 (MQ=11)

                                                                                                   aaTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTTATATCTTGTCTTTCTTACTTTAATATg                                            >  1:304752/1‑141 (MQ=11)

                                                                                                   aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                            >  1:358758/1‑141 (MQ=21)

                                                                                                    aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGa                                           >  1:166263/1‑141 (MQ=11)

                                                                                                    aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGa                                           <  2:163039/141‑1 (MQ=255)

                                                                                                    aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTAAGGCTATTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGa                                           >  1:352855/1‑141 (MQ=11)

                                                                                                    aTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAAtgtgt                                           >  1:106025/1‑140 (MQ=19)

                                                                                                      ttCGTGGCATGGGGAAAATCCGTAGAGCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATGCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg                                         <  1:127175/141‑1 (MQ=11)

                                                                                                      ttCGTGGCATGGGCAAAGTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTTCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg                                         <  1:25851/141‑1 (MQ=11)

                                                                                                       tCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACgg                                        <  1:289429/141‑1 (MQ=255)

                                                                                                       tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                        <  1:48683/141‑1 (MQ=38)

                                                                                                         gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACTTACTGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTATTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTg                                      <  1:101840/141‑1 (MQ=14)

                                                                                                          tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                     >  2:303568/1‑141 (MQ=255)

                                                                                                           ggCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCATAAATACAATCAATACCTTTTAAAGTATTCTTTGCCGAATAAATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    >  1:69150/1‑141 (MQ=11)

                                                                                                           ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    >  2:234769/1‑141 (MQ=38)

                                                                                                           ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    >  2:85693/1‑141 (MQ=37)

                                                                                                           ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    >  1:65540/1‑141 (MQ=255)

                                                                                                           ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    >  1:43998/1‑141 (MQ=255)

                                                                                                             cATGGCGAAAATCCGTAGATCTGAAGAGACCTCCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTTCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCt                                  <  1:250022/141‑1 (MQ=255)

                                                                                                             cATGGCGAAAATCCGTAGATCGGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTAGTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCt                                  <  1:52935/141‑1 (MQ=255)

                                                                                                               tGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                <  1:47451/141‑1 (MQ=255)

                                                                                                                ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAAGATGACGGTGATCTTGc                               <  1:185336/141‑1 (MQ=255)

                                                                                                                  cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTGTTATCT‑CGCACATTAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                             <  1:269721/141‑1 (MQ=14)

                                                                                                                    aaaaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCaa                           <  1:111954/141‑1 (MQ=255)

                                                                                                                     aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                          >  1:360555/1‑141 (MQ=38)

                                                                                                                     aaaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACAATCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                          >  1:248456/1‑141 (MQ=255)

                                                                                                                      aaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGGCGCTGATCTTGCTCAAta                         >  1:341864/1‑140 (MQ=255)

                                                                                                                      aaTCCGTAGATCTGAAGAGAACTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTGTCTTACTGTAATATGACGATGATCTTGCTCAATg                         >  1:225728/1‑141 (MQ=255)

                                                                                                                       aTCCGTAGACCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                        <  1:281277/141‑1 (MQ=255)

                                                                                                                         ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGTTACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCACTGAgg                      >  1:322545/1‑141 (MQ=255)

                                                                                                                          cGTAGATCTGAAGAGACCTGCTGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     <  2:224273/141‑1 (MQ=255)

                                                                                                                          cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     >  2:121985/1‑141 (MQ=38)

                                                                                                                          cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     >  1:327609/1‑141 (MQ=38)

                                                                                                                          cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     >  1:97327/1‑141 (MQ=255)

                                                                                                                             ataTCGGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAt                  <  2:277178/139‑1 (MQ=255)

                                                                                                                              gATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATACGACGGTGATCTTGCTCAATGAGGTTAtt                 <  1:240862/141‑1 (MQ=255)

                                                                                                                              gATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                 <  2:209556/141‑1 (MQ=255)

                                                                                                                                   gaggagaCCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGat            <  2:190055/138‑1 (MQ=255)

                                                                                                                                   gAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGat            >  1:276089/1‑141 (MQ=38)

                                                                                                                                      gagaCCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt         <  1:137838/141‑1 (MQ=38)

                                                                                                                                      gagaCCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt         <  1:186587/141‑1 (MQ=255)

                                                                                                                                       agaCCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAttt        <  2:360413/141‑1 (MQ=255)

                                                                                                                                       agaCCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAACTATTCTTTGCTGTATTGATACTTTGATACCTTCTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCATATAttt        <  2:234827/141‑1 (MQ=255)

                                                                                                                                        gaCCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTTTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTc       >  1:143411/1‑141 (MQ=255)

                                                                                                                                         aCCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAACGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg      >  1:272692/1‑141 (MQ=255)

                                                                                                                                         aCCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg      <  2:148816/141‑1 (MQ=255)

                                                                                                                                          ccTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa     <  2:118876/141‑1 (MQ=255)

                                                                                                                                          ccTACGCTTCTTTTTATAT‑AGCGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTCATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa     <  1:184784/141‑1 (MQ=11)

                                                                                                                                           cTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTCAAGTTTCATGTAGGTTATTTATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt    <  2:262933/141‑1 (MQ=14)

                                                                                                                                           cTGCGGTTCTTTTTATAT‑AGAGCGTAAATAAACTCAATACCTTTTAAAGTATTCTTTGCGGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt    >  2:217246/1‑141 (MQ=255)

                                                                                                                                            tGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATg   <  1:23504/141‑1 (MQ=38)

TGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35799‑36080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`