BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,015	C→T	35.0%	R179R (AGG→AGA)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,015	0	C	T	35.0%	349.7 / 260.1	311	R179R (AGG→AGA)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (98/102); new base T (54/55); total (154/157)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 321 total aligned reads displayed.

GCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATC  >  CP000730/35875‑36155

gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                                                                              <  2:49348/141‑1 (MQ=17)

     aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTCTTGATACTTTGATACCTTGt                                                                                                                                         >  2:196308/1‑141 (MQ=17)

       ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                                                                                                       <  2:64471/141‑1 (MQ=17)

        tGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctt                                                                                                                                      >  1:6175/1‑141 (MQ=255)

         gCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtcttt                                                                                                                                     <  1:45719/141‑1 (MQ=11)

         gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                                                                     <  2:106598/141‑1 (MQ=17)

         gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACATTGtcttt                                                                                                                                     >  1:10176/1‑141 (MQ=17)

         gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCACTCCAACTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                                                                     <  1:288150/141‑1 (MQ=11)

           tAGCATGATGCTAATTTCGTGGCATGAGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATAGATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                                                                                                                                   <  1:227971/141‑1 (MQ=11)

            aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGTTGTATTGATACTTTGATACCTTGcctttatt                                                                                                                                  >  2:220395/1‑141 (MQ=255)

             gCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGGTACTTGGATATCTTGtctttctgt                                                                                                                                 >  1:27449/1‑139 (MQ=11)

                tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                                                                                              >  1:354009/1‑141 (MQ=255)

                tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                                                                                              >  1:374363/1‑141 (MQ=11)

                   tgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTaa                                                                                                                           <  1:75992/141‑1 (MQ=21)

                    gCTAATTTCGTGGCATGGCGAAAATCAGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATAACTTTTAAAGTATTGTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                                                                                                                          <  1:226083/141‑1 (MQ=255)

                     cTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAata                                                                                                                         <  1:40514/141‑1 (MQ=255)

                     cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATAAACTCAATACCTTTTAAAGTATACTTTGCTGTATTGTTACTTTGATACCTTGTCTTTCTTACTTTAatt                                                                                                                         >  2:340586/1‑140 (MQ=255)

                      tAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCGTACTTTAatat                                                                                                                        >  1:232312/1‑141 (MQ=11)

                       aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                                                                       >  2:230225/1‑141 (MQ=35)

                        aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGa                                                                                                                      >  1:166263/1‑141 (MQ=11)

                         tttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGAc                                                                                                                     <  1:359891/141‑1 (MQ=11)

                          ttCGTGGCATGGGGAAAATCCGTAGAGCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATGCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg                                                                                                                    <  1:127175/141‑1 (MQ=11)

                           tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                                                                                                   <  1:340462/141‑1 (MQ=38)

                             gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                 <  1:217799/141‑1 (MQ=255)

                             gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                 <  1:239946/141‑1 (MQ=255)

                              tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                                                                                                >  2:303568/1‑141 (MQ=255)

                              gggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                                                                                                <  2:334242/140‑1 (MQ=255)

                                gCATGGCGAAAATCCGTAGATCTGCAGAGACCTGCGGTTTTTTTTGT‑CAGATCCTGAAATACACTCAATACCGTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATc                                                                                                              <  1:175336/141‑1 (MQ=14)

                                      cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTGTTATCTCGCACAT‑TAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                                                                                                        <  1:269721/141‑1 (MQ=14)

                                        aaaaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCaa                                                                                                      <  1:111954/141‑1 (MQ=255)

                                         aaaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACAATCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                                                                     >  1:248456/1‑141 (MQ=255)

                                           atcctgaGATCTGAAGGGACCTGCGGTTCTTTTTATATAGTGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                                                                                                   <  2:248416/135‑1 (MQ=14)

                                           aTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATGCTTTGATCTCTTGTCTTCCTTCCTCTCATCTATCGGTGATCTTGCTCAATGa                                                                                                   >  1:67203/1‑141 (MQ=11)

                                           aTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGTGCTTGCTCAAtag                                                                                                   >  1:250038/1‑139 (MQ=11)

                                            tCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAg                                                                                                  <  2:276054/141‑1 (MQ=255)

                                                      tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTTCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                                                                        <  1:78070/141‑1 (MQ=37)

                                                       gAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGat                                                                                       >  1:276089/1‑141 (MQ=38)

                                                         agagACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGatat                                                                                     <  1:52148/141‑1 (MQ=255)

                                                          gagaCCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGGTGTATTGATACTTTGATACCTTGTCTTTCTTGCTTTGATATGACGGTGGTCTTGGTAAATGAGGTTATTCAGATAtt                                                                                    >  1:191916/1‑141 (MQ=11)

                                                          gagaCCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                                                                    <  1:137838/141‑1 (MQ=38)

                                                          gagaCCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                                                                    <  1:186587/141‑1 (MQ=255)

                                                           agaCCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAttt                                                                                   <  2:360413/141‑1 (MQ=255)

                                                             aCCTACGGTTCTTTTTATATCGAGAGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg                                                                                 <  2:178502/141‑1 (MQ=14)

                                                             aCCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg                                                                                 <  2:232153/141‑1 (MQ=255)

                                                              ccTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa                                                                                <  2:118876/141‑1 (MQ=255)

                                                                 acGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTCATCTTTGCCGTATTGATACTTTGATTTATTGATAAATGTGTTATAATAGGACGGTTTGCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                                             >  2:262994/2‑141 (MQ=2)

                                                                 acGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                                             <  1:330142/140‑1 (MQ=255)

                                                                    gTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACa                                                                          <  1:221361/141‑1 (MQ=255)

                                                                       cTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCGGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TATTCAGATATTTCGATGTACAATgg                                                                      >  1:9167/1‑140 (MQ=255)

                                                                       cTTTATATATAGAGCGT‑AAATACATTCAATGCCTTTTAAAGTATTCCTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATAGTACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATg                                                                       >  2:226303/1‑141 (MQ=14)

                                                                            tatataGAGAGT‑AAATACATTCAATACATTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGt                                                                  <  2:244234/141‑1 (MQ=255)

                                                                            gatataGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                                                                  <  2:70933/140‑1 (MQ=255)

                                                                             atataGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTAGTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGtt                                                                 >  1:271817/1‑140 (MQ=255)

                                                                              tataGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCa                                                                <  1:209561/141‑1 (MQ=255)

                                                                              tataGAGCCT‑AAATCCTCTCAATACTTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCa                                                                <  1:142164/141‑1 (MQ=14)

                                                                              tacagagCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCa                                                                <  1:106505/138‑1 (MQ=255)

                                                                                  gagCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtt                                                            >  1:26437/1‑141 (MQ=40)

                                                                                  gagCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGACCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtt                                                            <  1:292454/141‑1 (MQ=255)

                                                                                  gagCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGACACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtt                                                            <  2:215839/141‑1 (MQ=255)

                                                                                   agCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgttt                                                           >  1:185110/1‑141 (MQ=255)

                                                                                     cGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaa                                                         >  2:16945/1‑141 (MQ=255)

                                                                                      gT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag                                                        >  2:305012/1‑141 (MQ=255)

                                                                                       t‑AAATACTCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagt                                                       <  2:363281/141‑1 (MQ=255)

                                                                                       t‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagt                                                       <  2:37809/141‑1 (MQ=255)

                                                                                          tcttcATTCACTTCTTTTTATAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTGCTTTAATATGACGGTGATCTTGCTCAATGTGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                                     <  2:148337/137‑1 (MQ=14)

                                                                                           aTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                                    >  2:345793/1‑141 (MQ=255)

                                                                                             acacTCAATACCGTTTAAAGTATTCTTTGCTTTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCACATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaa                                                  <  2:322015/141‑1 (MQ=14)

                                                                                              cATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTaaaa                                                 <  1:243759/141‑1 (MQ=255)

                                                                                               attgagtACCTTTTAAAGTATGCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTGCTTTGATATGACGGTGATCTTGCTCGATGTGGTTATTCAGTTATTTCGATGTACTATGACAGTCAGGTGTAAGGTTAAAAg                                                <  2:85516/135‑1 (MQ=255)

                                                                                               actCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAg                                                >  1:335265/3‑141 (MQ=255)

                                                                                                ttCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAgt                                               >  1:97606/1‑140 (MQ=255)

                                                                                                ctCAATACCTTTTAATGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGc                                               <  2:365670/140‑1 (MQ=255)

                                                                                                ctCAATACCTTTTAAATTATTCTTTGCTGTATTGATACTTTGATACCTTGTATTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGc                                               <  1:85693/140‑1 (MQ=255)

                                                                                                 taaagttcTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATACGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCt                                              <  2:277828/134‑1 (MQ=255)

                                                                                                   aaTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCttt                                            <  2:320397/141‑1 (MQ=40)

                                                                                                   aaTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTATCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGCTTAAGTTTAAAAGCttt                                            >  2:237443/1‑141 (MQ=255)

                                                                                                    aTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTa                                           >  1:302862/1‑141 (MQ=255)

                                                                                                    aTACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTa                                           <  2:185131/141‑1 (MQ=255)

                                                                                                        cTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCCGTCAGGTTTAAGTTTAAAAGCTTTAATTa                                       >  1:200934/1‑141 (MQ=255)

                                                                                                          tttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACt                                     <  1:306864/141‑1 (MQ=255)

                                                                                                             aaaGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGATGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTa                                  <  2:40838/141‑1 (MQ=255)

                                                                                                                gTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAAcc                               >  2:201610/1‑141 (MQ=255)

                                                                                                                gTATTCCTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAAcc                               >  2:205381/1‑141 (MQ=255)

                                                                                                                 tATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCa                              >  1:145559/1‑141 (MQ=255)

                                                                                                                   ttCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCAtt                            >  1:318953/1‑141 (MQ=255)

                                                                                                                     cTTTGCTGTATTGATACGTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGc                          <  2:97327/141‑1 (MQ=255)

                                                                                                                     cTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGc                          <  2:160914/141‑1 (MQ=255)

                                                                                                                       ttGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCtg                        >  2:240015/1‑140 (MQ=255)

                                                                                                                         gccgTATTGATACTTTGATATATTGACAGTTTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGcc                      >  1:371310/4‑141 (MQ=14)

                                                                                                                           cgTATTGATTCCTTGTTTTATTGTCTAGCTTGCTTTAAGATGACGGTGTTCTTGCTCTTTGAGTTTTTTCAGATATTCCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCtt                    <  2:352814/140‑1 (MQ=2)

                                                                                                                              aTTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGt                 <  1:58800/141‑1 (MQ=255)

                                                                                                                                    aCTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAgg           >  2:126737/1‑141 (MQ=255)

                                                                                                                                    aCTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAGTGAGGTTATTCCGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAgg           >  2:101474/1‑141 (MQ=255)

                                                                                                                                     cTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGt          <  1:121985/141‑1 (MQ=255)

                                                                                                                                       ttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGc        <  2:287963/141‑1 (MQ=255)

                                                                                                                                       ttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGc        <  2:138473/141‑1 (MQ=255)

                                                                                                                                        tGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGcc       >  1:282106/1‑141 (MQ=255)

                                                                                                                                         gATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCt      >  2:315077/1‑141 (MQ=255)

                                                                                                                                         gATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCt      >  1:323816/1‑141 (MQ=255)

                                                                                                                                         gATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGACATTGCTAACTTCGTTGAGGGTGCCt      >  2:253505/1‑141 (MQ=255)

GCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATC  >  CP000730/35875‑36155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`