BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,413	C→T	55.4%	V47I (GTC→ATC)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,413	0	C	T	55.4%	85.3 / inf	548	V47I (GTC→ATC)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (124/120); new base T (158/144); total (284/264)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.62e-01

Only 100 of 552 total aligned reads displayed.

ATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCAT  >  CP000730/36274‑36551

aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATACACAATTGGGGCATATTCTTGAACCCAACGGTAGAcc                                                                                                                                           >  2:66445/1‑141 (MQ=255)

aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGAcc                                                                                                                                           <  1:272003/141‑1 (MQ=18)

 tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCg                                                                                                                                          >  1:684038/1‑141 (MQ=21)

  ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGt                                                                                                                                         >  2:638343/1‑141 (MQ=39)

   gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGtt                                                                                                                                        >  1:398997/1‑141 (MQ=255)

    cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTg                                                                                                                                       <  1:362359/141‑1 (MQ=21)

     gcGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCCTTTGTAATAAGCTTTTATATTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                                                      <  2:193841/140‑1 (MQ=255)

     ccGATATAAATAGCTCCATTTTCCGTTTCTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                                                      <  2:575408/140‑1 (MQ=255)

     aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                                                      >  1:503330/1‑141 (MQ=255)

      cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCTAAAATTGGGGCATATTATTGAACCCAACGGTAGATCGTTGaa                                                                                                                                     >  2:766887/1‑141 (MQ=11)

      cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                                                                                                                     >  2:721025/1‑141 (MQ=255)

      cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                                                                                                                     >  1:749143/1‑141 (MQ=255)

       gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAat                                                                                                                                    >  1:598636/1‑141 (MQ=255)

       gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAat                                                                                                                                    >  1:121119/1‑141 (MQ=255)

        atatAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatg                                                                                                                                   <  2:749484/141‑1 (MQ=21)

          aaaaaTAGCCCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgat                                                                                                                                 <  2:279769/139‑1 (MQ=11)

           taAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgatg                                                                                                                                >  1:218550/1‑141 (MQ=255)

           taAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgatg                                                                                                                                >  1:86554/1‑141 (MQ=255)

            aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                                                               >  1:45866/1‑141 (MQ=255)

            aaaTAGCTCCATTTTCCTTTGATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTATACCGTTGAAtgatga                                                                                                                               <  1:584073/141‑1 (MQ=255)

               aaGCTCCATTTTCCTTTTATTTTGATATACGTCCCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACg                                                                                                                            <  2:507782/140‑1 (MQ=255)

                aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCCATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGt                                                                                                                           <  2:624038/141‑1 (MQ=255)

                aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGt                                                                                                                           >  1:724753/1‑141 (MQ=255)

                aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAAGt                                                                                                                           >  2:287523/1‑141 (MQ=255)

                  cTCCATCTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGttt                                                                                                                         <  1:762107/141‑1 (MQ=21)

                    tcATTTTCCTTTTATTTTGATGTACGTTTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTac                                                                                                                       <  1:667750/140‑1 (MQ=21)

                    ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATTAACGTTTac                                                                                                                       >  1:193112/1‑141 (MQ=255)

                     cATTTTCCTTTTATTTTTATATACGTCTCATCAATACGTCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATCGGAGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                                                      <  1:541966/141‑1 (MQ=11)

                     cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                                                      >  1:293595/1‑141 (MQ=255)

                      aTTTTCCTTTTATTTTGATGTACGTCCCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGGTGAATGATGAACGTTTacac                                                                                                                     <  1:479313/141‑1 (MQ=255)

                       ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTATCTTCCAAATTTGATATAACCCCGGGACTTCATCAGAAACCAAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                                                    >  1:372503/1‑141 (MQ=11)

                       ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                                                    <  2:431913/141‑1 (MQ=255)

                       ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGCGGCATATTCTTGAACCCAACGGTAGATCGTTGAAGGATGACCATTTAcacc                                                                                                                    >  2:445235/1‑141 (MQ=11)

                       tttgCCTTTTCTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTGTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                                                    <  2:490790/141‑1 (MQ=255)

                         ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                                                                  >  1:489681/1‑141 (MQ=255)

                         ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTATTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATAAAGGTTTAcaccac                                                                                                                  >  1:308742/1‑141 (MQ=11)

                           ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGCTGAACGTTTACACCACGt                                                                                                                >  2:713354/1‑141 (MQ=255)

                           ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                                                                <  2:102668/141‑1 (MQ=255)

                           ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTCGGGATTTTTCAGGAACCCAACGGTAGATCCTTGCATGATGAACGTTTACACCACGt                                                                                                                >  1:179533/1‑141 (MQ=11)

                            tttttATTGTGATGAACGTCTCAGCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt                                                                                                               <  1:692757/140‑1 (MQ=255)

                            cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt                                                                                                               >  1:471990/1‑141 (MQ=21)

                            cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt                                                                                                               >  1:715930/1‑141 (MQ=21)

                            cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCATTGAATGATGAACGTTTACACCACGtt                                                                                                               >  2:83788/1‑141 (MQ=25)

                            cTTTTATTTTGATGTACGTCTCATCAATACGCCATTGGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAAACCGTTGATTGATGAACGTTTCAACCACGtt                                                                                                               >  1:546068/1‑141 (MQ=11)

                            cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                                                               >  1:740373/1‑141 (MQ=255)

                            cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                                                               >  1:516300/1‑141 (MQ=255)

                            cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                                                               >  1:205802/1‑141 (MQ=255)

                             ttttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTc                                                                                                              >  1:385627/1‑141 (MQ=255)

                               ttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                                                            <  2:255869/141‑1 (MQ=21)

                               ttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                                                            >  1:284542/1‑141 (MQ=18)

                                 aTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                          <  2:599218/141‑1 (MQ=18)

                                  ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCGTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                                                         <  2:402079/141‑1 (MQ=11)

                                  ttttGATATACGTCCCGTCAATGCGCCCTTCCTGAAAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATAATGAACGTTTACACCACGTTctcgta                                                                                                         >  1:156182/1‑136 (MQ=11)

                                    ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAat                                                                                                       <  2:130617/141‑1 (MQ=255)

                                     tGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAata                                                                                                      <  1:230854/141‑1 (MQ=21)

                                     tGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAata                                                                                                      >  1:426328/1‑141 (MQ=25)

                                     tGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAata                                                                                                      <  1:5337/141‑1 (MQ=21)

                                      gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGGTTACACCCCGTTCTCTTAatat                                                                                                     >  1:101883/5‑141 (MQ=11)

                                      gATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                     <  1:301258/141‑1 (MQ=255)

                                          tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                 <  1:603880/141‑1 (MQ=21)

                                          tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                 >  2:146127/1‑141 (MQ=25)

                                          tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                 <  1:2265/141‑1 (MQ=18)

                                          tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                 >  1:710064/1‑141 (MQ=25)

                                           aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                                                                >  2:394665/1‑141 (MQ=255)

                                             gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                                                              <  2:339020/141‑1 (MQ=255)

                                               ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGCACCCACCGGGAGACCGTTGAATGATGAACGTTTACACCACGTCCTCTTAATATTTCAGatat                                                                                            >  2:240771/1‑141 (MQ=11)

                                                tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTCCTCTTAATATTTCAGatata                                                                                           >  1:638205/1‑141 (MQ=255)

                                                tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCGTTCCCTTAATCTTTCAGataca                                                                                           >  1:62396/1‑139 (MQ=255)

                                                  atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGTTGAACGTTTACACCAGGTTTTTTTAGTATTTCAGATATATc                                                                                         >  1:437433/1‑141 (MQ=11)

                                                  atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                                                         >  1:557806/1‑141 (MQ=25)

                                                   tcaATACGCCATTTGTAATAAGCTGTTTTATGCTTTTTCTTTCGAATTGTAGATAAAAGTGGGGCATATTCTTGAACCCACCGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                                                        <  2:165226/141‑1 (MQ=11)

                                                    caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATCAAATTGGGGCATATTCTCAAACACACCGGTAGATCGTTGAATGATGCACGTTTCCACCACGTTTTCTTAATATTTCAGCTATATCag                                                                                       >  1:355155/1‑140 (MQ=11)

                                                    caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                                                       >  2:380533/1‑141 (MQ=255)

                                                      aTACGCCATTTGTAATAAGCTATTTTATGCTTTTTCTTCAAAATTTGATATAAAATTAGGTCATATTCTTGAACACAACGGTAGATCGTTGAATAATGCACGTTTACAACACGTTCCCCAAATTTTTCATTAATATCAcca                                                                                     >  2:108663/1‑139 (MQ=255)

                                                      aTACGCCACTTGAAATAAGCTTTTTCATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGTTAGATCGTTGAATGATGAACGTTTACACCCCGTTCTCTTAATATGTCAGATATATCACGa                                                                                     <  1:258403/141‑1 (MQ=11)

                                                       tACGCCATTTGGTATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCCACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                                                    <  2:309735/141‑1 (MQ=11)

                                                       tACGACATTTGTAATAAGCTTTTTTATGCTATTTCTTCCAAATTTGATATAAATTTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACAACGTTCTCTTAATATTTCAGatatataccgaa                                                                                    >  2:481284/1‑135 (MQ=11)

                                                        accccATGTGTAATAAGCGTTTTTACACATATTCTGCCAAATTGGACACTGATTTGCGTCATATTCATGACACCAACTGTAGTCCGCTCAAGGTTGAACGGGTACAACACGTTCCCTTAATATTTCAGATATATCAcaaga                                                                                   <  2:321717/138‑5 (MQ=2)

                                                         cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAACTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACTCCACGTTTCTTTAATTTTTTAGATATATCACGGTaa                                                                                  >  1:80076/1‑141 (MQ=11)

                                                         cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATTTTTCAGATATATCACGATag                                                                                  <  1:44753/141‑2 (MQ=11)

                                                         cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                  >  1:76936/1‑140 (MQ=255)

                                                         cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTAAGATATATCACGATag                                                                                  >  2:631795/1‑140 (MQ=255)

                                                          gCCATTTGTAATAAGCTTTTTGATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                                                                 <  1:13807/141‑1 (MQ=17)

                                                           ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGGTATAAAAGTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                >  1:416904/1‑141 (MQ=11)

                                                           ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                >  1:565915/1‑141 (MQ=11)

                                                              tttCTAATAAGCTTTTTCTTGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTCGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                             <  2:528253/141‑1 (MQ=11)

                                                              gttGTAATAAGCTGTATTATGCTTGTTCTACCAAATTTGATACAAAATTGGGGCATATTCAGGAACCCAACGATAGACCGATGAATGATGAAGGTTTACACACCGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                             >  2:353346/2‑141 (MQ=11)

                                                               ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                            <  1:648721/141‑1 (MQ=255)

                                                               ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTATTAATATTTCAGAAATATCACGATAGCGCAAt                                                                            >  2:615159/1‑141 (MQ=11)

                                                               ttGTAATAAGCTTTTTTATGCTTTTTCTCCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                            <  2:55673/141‑1 (MQ=11)

                                                                 ttaataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                          <  2:225225/140‑2 (MQ=11)

                                                                  taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGtt                                                                         >  2:535678/1‑140 (MQ=255)

                                                                   aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCCGATATATCACGacagaccaatgcat                                                                        >  2:471345/1‑128 (MQ=11)

                                                                   aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGATCTCTTAATATTTCAGATATATCACGATAGCTCAATgcaa                                                                        >  2:685558/1‑138 (MQ=11)

                                                                   aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCCCGATAACTCATTGtaa                                                                        >  2:268896/1‑140 (MQ=255)

                                                                    ataaGCTTTTTTATGCTTTATATTGCACATTTGATATAAACTTGGGGGAGATTAGAGAACCCGAAGATGGATCGGTGAGTGATGAACGTTTAAGAGACGTTATCTTAATATTTCAGATACATCACGATAGCGCaagatatt                                                                       >  2:204848/1‑135 (MQ=255)

                                                                                    tcttgcttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCa                                                       <  1:241332/136‑1 (MQ=25)

                                                                                            ccAAAGTTGATACAGAGTGTGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTAc                                               <  2:123005/141‑1 (MQ=11)

                                                                                                                 ggCAAATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGGTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGCTaaa                          >  2:545530/1‑141 (MQ=12)

                                                                                                                               cccAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTAAGATATTGCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGAGATAACATCCTTTTTAACTTGTTTATATCTGa            >  1:327676/1‑141 (MQ=11)

ATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCAT  >  CP000730/36274‑36551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`