Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,180 C→T 36.4% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,1800CT36.4% 176.1 / 101.3 119intergenic (+238/‑896)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (27/47);  new base T (17/25);  total (45/74)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.48e-02

Only 100 of 120 total aligned reads displayed.

TGAATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAAT  >  CP000730/76040‑76315
                                                                                                                                            |                                                                                                                                        
       aaTATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGta                                                                                                                                   <  1:563086/141‑1 (MQ=255)
              cTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAtttt                                                                                                                            <  1:550027/141‑1 (MQ=255)
               tAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTa                                                                                                                           >  1:9303/1‑141 (MQ=255)
                aaTATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTaa                                                                                                                          <  1:137496/141‑1 (MQ=255)
                 atatTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTaaa                                                                                                                         >  2:225327/1‑140 (MQ=255)
                     tgctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTCACaaaa                                                                                                                     >  1:211573/1‑141 (MQ=255)
                      gctgcACCTGTATCAGTTAGAACTACTCACCAAAGTGGTAGTCATCGAGGGAATAAAAACACAAAGGCAATTAATTTAATAGTTACAGCAAAAAACAGGTCCTGTTGAAAAGTTGAAACCATAAAATAATTTTaaaaaaaa                                                                                                                    >  2:16827/1‑141 (MQ=255)
                      gctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACaaaaa                                                                                                                    <  2:334920/141‑1 (MQ=255)
                      gctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACaaaaa                                                                                                                    <  1:681868/141‑1 (MQ=255)
                      gctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGAGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACaaaaa                                                                                                                    <  1:70596/141‑1 (MQ=255)
                       ctgaACATGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAg                                                                                                                   <  1:398932/141‑1 (MQ=255)
                        tgtacatGTATCACTTAAAACAGCTAACCAAAGTGTTAGTAATCCAGTTAATACTAATACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAATTTGAATCTATAGTATAATTTTAACaaaaatg                                                                                                                  <  2:692749/135‑3 (MQ=38)
                               gTAACACTTAGAACCGCCAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGATGCAACGTTGTATATTAACTATAATTTTAACATAAAGGAGtcttc                                                                                                           >  2:163546/1‑141 (MQ=255)
                                tATCACTTAGAACAGCTAACCAAAGTGCTAGTAACACCAGACACACAAACACAAATACCATTACTTTAATAATTATAGCTAAATATAGGTTCTGTTCCCAAGTTGAACCTAGAGTAAAATTTTAACAAAAATGAGacctct                                                                                                          >  2:130175/1‑141 (MQ=255)
                                tATCACTAAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTAAAAAATAAAAAAAAAGGAGtcttct                                                                                                          <  2:566060/141‑1 (MQ=39)
                                      ttAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGa                                                                                                    <  1:180167/141‑1 (MQ=255)
                                       tAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCGATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGaa                                                                                                   <  1:507264/141‑1 (MQ=255)
                                        aGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAAc                                                                                                  <  1:235302/141‑1 (MQ=255)
                                        aGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAAc                                                                                                  <  1:589930/141‑1 (MQ=255)
                                          aaCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCAGTATGAACTa                                                                                                >  1:90328/1‑141 (MQ=255)
                                           aCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAt                                                                                               <  1:656227/141‑1 (MQ=255)
                                           aCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTGCTGTATGAACTAt                                                                                               >  2:656268/1‑141 (MQ=255)
                                           aCAGCGAACCAATGTGTTAGTAATCCAGGGAATCCTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATTGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAt                                                                                               <  1:348060/141‑1 (MQ=255)
                                              gCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGACAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTc                                                                                            >  2:266785/1‑141 (MQ=255)
                                                 aaCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATACTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCaca                                                                                         >  2:110569/1‑139 (MQ=255)
                                                  aCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAtttacgag                                                                                        >  1:566722/1‑136 (MQ=255)
                                                         tgtTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACa                                                                                 >  2:27301/1‑141 (MQ=255)
                                                             aatAAACCAGGAAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                            <  2:708588/139‑1 (MQ=255)
                                                             aGTAATCCATGGAATACTAACACAAAGGCAATTAACCTAATAATTATAGCAAAATACAGGTCCTGTTGCAAAGTTGAATCTATAGTAAAAAAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                            <  2:617408/141‑1 (MQ=21)
                                                             aGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                            >  2:424750/1‑141 (MQ=255)
                                                                  tCCTGGGAATACTAACACAAAGGCATTTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACaa                                                                       <  1:238513/141‑1 (MQ=255)
                                                                                acacAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTg                                                         <  1:303635/141‑1 (MQ=255)
                                                                                 cacaAAGGCAATTAACTTAAATATTACAGCAAAATATATGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCCTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGt                                                        <  1:319662/141‑1 (MQ=17)
                                                                                   caAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAg                                                      <  1:118058/141‑1 (MQ=255)
                                                                                   caAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAg                                                      <  1:195968/141‑1 (MQ=255)
                                                                                     aaGGCAATTACTTTAATAATTCTAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                    <  1:172888/141‑1 (MQ=255)
                                                                                     aaGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                    <  1:173017/141‑1 (MQ=255)
                                                                                     aaGGCAATTAATTTAATAATTATAGAAAAATATAGGTTCTTTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                    <  2:221452/141‑1 (MQ=255)
                                                                                      aGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCg                                                   >  1:399516/1‑141 (MQ=255)
                                                                                       ggCAATTAATTTAATAATTATAGCAAAATATAGGTACTGTTGCAAAGTTGAATCTATAGTATAATATTAACAAAAAGGAGTCTGCGGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGt                                                  >  2:228296/1‑141 (MQ=255)
                                                                                          aattaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAGAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTATGTATGAACTCTTTCAGACATAAACAATTAAACAAGGATGTTATCACTGTAGCCGTTgg                                               <  2:659840/141‑1 (MQ=17)
                                                                                             taatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAGTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGAGATAAACAATTTAACAAGGATGTTATCACTGTATCCGTTGGcta                                            >  1:166452/1‑141 (MQ=255)
                                                                                             taatatgatAATGAGAGGAAAAAAGAGGTTCTGTTGGAAAGCTGAATCTATATTAAAAAAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGcta                                            <  2:382085/141‑1 (MQ=11)
                                                                                             aaatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGcta                                            <  1:279577/140‑1 (MQ=255)
                                                                                                   aataatTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGCAGGTAGGATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCt                                      >  1:574299/1‑141 (MQ=17)
                                                                                                    ataatTATAGCAAAATATTGAGTCTGGTGCAAAGTTGATTCTATAGTATAATTATAAAAAAAAGGAGTCTTCTGTATGTACTATTTCAGATATAACCCATTTAACAAGGATGTTATCACTGTAGACGTTGGCTACTATCTa                                     <  2:289655/141‑1 (MQ=14)
                                                                                                    ataaaTATAGGCAAACATAGGTTCTGTTGTAAAGTTGAATCTATTGTATAAAAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTa                                     <  2:224301/141‑1 (MQ=11)
                                                                                                        ttATAGCAAAATATAGGTTCTGTTGCAAAGTTCAATCTATAGTATAATTTTAACAAAAAGGAGTCCTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGat                                 <  1:526604/141‑1 (MQ=255)
                                                                                                          ataGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTATGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTAATATGCAAGatat                               >  2:621059/1‑141 (MQ=255)
                                                                                                              cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTCTCACTGTAACTGTTGGCCACTATCTAAGATATGCAt                           >  1:530289/9‑141 (MQ=1)
                                                                                                              cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                           >  1:645850/9‑141 (MQ=1)
                                                                                                              cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                           >  1:156821/9‑141 (MQ=11)
                                                                                                              cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                           >  1:238094/9‑141 (MQ=1)
                                                                                                              aataaatgaGGTTCTGGTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAt                           <  2:751178/133‑1 (MQ=11)
                                                                                                               gtaaatgaGGTTCTGTTGCAAAGTTGAATTTATGGTATAATTTTAACAAAAGGGGGTATTCTGTATGAACTATTTCAGAGAGATACGATTTAGCACGGCTGTTATCAAACTAGATGTTGGCTGCTATCTAAGATATGCAtt                          >  2:674139/8‑141 (MQ=2)
                                                                                                                taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                         <  2:19210/135‑1 (MQ=2)
                                                                                                                taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                         <  2:409961/135‑1 (MQ=255)
                                                                                                                taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATAGGCATTg                         >  1:305848/7‑141 (MQ=1)
                                                                                                                taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTAAAATAATACAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                         <  2:404896/135‑1 (MQ=11)
                                                                                                                gttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                         <  1:47979/135‑1 (MQ=0)
                                                                                                                gttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                         <  2:768824/135‑1 (MQ=255)
                                                                                                                aaaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                         <  2:333122/141‑1 (MQ=40)
                                                                                                                  taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                       <  2:649000/137‑1 (MQ=9)
                                                                                                                  taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                       <  2:469028/137‑1 (MQ=2)
                                                                                                                  atatAGGTTCTGTTGTAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                       <  1:236609/141‑1 (MQ=255)
                                                                                                                  atatAGGTTCTGGTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                       <  1:9816/141‑1 (MQ=255)
                                                                                                                  aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                       <  2:146789/137‑1 (MQ=2)
                                                                                                                   atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                      <  1:744661/138‑1 (MQ=9)
                                                                                                                   aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                      >  1:423638/4‑140 (MQ=0)
                                                                                                                   aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                      <  1:303162/138‑2 (MQ=2)
                                                                                                                   aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                      >  1:206619/4‑140 (MQ=2)
                                                                                                                   aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                      <  1:70770/138‑2 (MQ=9)
                                                                                                                   aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGACTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                      <  2:52961/138‑2 (MQ=0)
                                                                                                                    tgaGGTTCTGATGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGtt                     <  2:550254/139‑1 (MQ=2)
                                                                                                                    ggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                     >  1:147746/3‑139 (MQ=0)
                                                                                                                    ggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                     >  1:558877/3‑139 (MQ=2)
                                                                                                                    ggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                     >  1:135622/3‑139 (MQ=2)
                                                                                                                    ggaGGTTCTGTTGCAAAGTTGAATTTATAATAAAAAAATAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                     <  2:486422/139‑3 (MQ=11)
                                                                                                                    ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATGTTCATTGAggt                     >  1:94504/1‑139 (MQ=255)
                                                                                                                     gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                    >  2:267132/2‑141 (MQ=14)
                                                                                                                     gaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgcta                    <  2:501687/140‑4 (MQ=2)
                                                                                                                     gaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgcta                    <  1:214950/140‑4 (MQ=2)
                                                                                                                      aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                   >  1:120858/1‑141 (MQ=1)
                                                                                                                      aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATACCAGTTCTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                   >  1:573401/1‑141 (MQ=11)
                                                                                                                      aGGTTCTGCTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTATTCTGTATGAACTATTTCAGATATAAACAATTTAAGAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                   >  2:582534/1‑141 (MQ=1)
                                                                                                                       ggTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATc                  >  1:335508/1‑141 (MQ=35)
                                                                                                                       ggTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATc                  <  2:76154/141‑1 (MQ=35)
                                                                                                                        gTTCTGTTGCAAAGTTGACACTCTATTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATAACTGTAGCTGTTGCCTACTATCTAAGATATGCATTGAGTTAtgg                 >  2:761403/1‑139 (MQ=11)
                                                                                                                         ttCTGTTGGAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGt                <  2:336042/141‑1 (MQ=25)
                                                                                                                         ttCTGTTGCAAAGTTGAATCTCTAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGt                <  1:253568/141‑1 (MQ=25)
                                                                                                                         tactgctgCAAAGTTGAATGTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGt                <  2:735252/139‑1 (MQ=11)
                                                                                                                          tCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCCTTGTGTTACCGTg               <  2:682212/141‑1 (MQ=255)
                                                                                                                             gtcgCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGata            <  1:717754/138‑1 (MQ=1)
                                                                                                                              ttccAAAGTTAAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTCATCGTGatat           <  2:288916/138‑1 (MQ=1)
                                                                                                                                tgaaaGTGGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGatatat         <  2:776085/139‑1 (MQ=255)
                                                                                                                                gCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatatat         >  2:250451/1‑141 (MQ=34)
                                                                                                                                gCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATAGTGatatat         >  1:768242/1‑141 (MQ=25)
                                                                                                                                    aggtGAATGTAGAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGa     <  1:156062/138‑1 (MQ=11)
                                                                                                                                     gTTGAATCTATAGTAGAATTTTAACAAAAAGGAGTCTTTTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCATTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaa    <  2:381797/141‑1 (MQ=255)
                                                                                                                                       tGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGCATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAat  <  1:653370/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                        
TGAATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAAT  >  CP000730/76040‑76315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: