Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,353 G→A 52.9% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,3530GA52.9% 44.0 / inf 498intergenic (+411/‑723)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (149/87);  new base A (122/139);  total (272/226)
Fisher's exact test for biased strand distribution p-value = 3.02e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

Only 100 of 499 total aligned reads displayed.

GTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCAT  >  CP000730/76213‑76491
                                                                                                                                            |                                                                                                                                           
gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACgg                                                                                                                                             <  1:183673/141‑1 (MQ=17)
  aTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTc                                                                                                                                           <  2:726277/141‑1 (MQ=17)
  aTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTc                                                                                                                                           <  1:501120/141‑1 (MQ=32)
     aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                                                                                                        >  1:54544/1‑141 (MQ=12)
      aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAACGAGAACAAGCATAAAAAAGAAGGCAACGTGGTGTAAACGTTCATCATTCAACGGTCTAcc                                                                                                                                       >  2:359597/1‑141 (MQ=11)
      aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTCTTGGCTACTATCTAAGATATGCATTGAATTATCGTGATATATCTGAAATAATAAGGGAACGTGGTGTAAACGTTCATCATTCCACGGTCTAcc                                                                                                                                       >  1:317627/1‑141 (MQ=11)
       cTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCg                                                                                                                                      >  1:746344/1‑141 (MQ=17)
        tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                                                                                                                     <  1:416145/141‑1 (MQ=255)
        tATTGCAGATATAAAGAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGt                                                                                                                                     <  2:707420/141‑1 (MQ=12)
        caTTTCAGATATAAACAATTTAACAAGGATGTTATCACCGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                                                                                                                     <  1:217382/140‑1 (MQ=11)
         ttttcggtttttaaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                                                                                    <  2:587392/129‑1 (MQ=11)
         aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                                                                                                                    <  2:239634/141‑1 (MQ=17)
         aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                                                                                                                    >  2:81575/1‑141 (MQ=32)
         aTTTCAGATATAAACAATATAACCAGGTTGTTATTACTGTAGCTGTTCGCTACAATGTGGAGGGTGCATGGTGTTAGCGGGATATGTCTTAATTAGTTACTTGACGTGGTGGAAACGTTCATCATTCAACGGTCTACCGtt                                                                                                                                    <  2:458365/141‑1 (MQ=2)
          tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTAAACGGTCTACCGTTg                                                                                                                                   >  2:546068/1‑141 (MQ=17)
          tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAATGATCTACCGTTg                                                                                                                                   <  2:55428/141‑1 (MQ=11)
           ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTgg                                                                                                                                  >  1:572186/1‑141 (MQ=17)
           ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGAAGTTGGCTAATATCTAAGATATGAATAGAAAACACGTGATATATATGAAATATTAAGAGAAAGCGGTGTAAACGTTCATAATTCAACGATCTACTGtgag                                                                                                                                  >  2:219575/1‑138 (MQ=2)
            tCAGATATAAACAATTTTACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGGGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                                                 <  1:760937/141‑1 (MQ=11)
            tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTGCATCATTCAACGGTCTACCGTTggg                                                                                                                                 >  1:101615/1‑141 (MQ=17)
            tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                                                 >  2:321881/1‑141 (MQ=12)
            tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                                                 >  1:304812/1‑141 (MQ=12)
              aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGtt                                                                                                                               >  1:196302/1‑141 (MQ=17)
                atatAAAAAATTCAACAAGGATGTTATCACTGTAGGCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCa                                                                                                                             <  1:27301/141‑1 (MQ=12)
                  ataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAg                                                                                                                           <  1:565392/141‑1 (MQ=255)
                   taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                                                                                                          <  1:339095/141‑1 (MQ=32)
                   taAACAATTTAACAAGGATGTAATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATAAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                                                                                                          <  1:380303/141‑1 (MQ=11)
                    aaaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGaa                                                                                                                         >  2:777717/1‑141 (MQ=17)
                    aaaCAAGTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGaa                                                                                                                         <  2:387153/141‑1 (MQ=255)
                     aaCAATTTAACAAGGATGTTATCACTGTAGCCTTTGGCTACTATCTAAGATATGCCTTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAat                                                                                                                        >  1:22832/1‑141 (MQ=11)
                     aaCAATTTAACAAGGATGTTATCACTGTAGCCGTCAGCTACTATCTAAGATATGCCTTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGGAAACGCTCATGATTCAGGCATCTACCGTTggggacaaaaga                                                                                                                        >  2:135804/1‑132 (MQ=11)
                      aCAATTTAATAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAAATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAata                                                                                                                       >  2:506144/1‑141 (MQ=11)
                      aCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAata                                                                                                                       >  1:150622/1‑141 (MQ=17)
                      aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAata                                                                                                                       <  1:785745/141‑1 (MQ=255)
                       cAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAAAGTTCATCATTCAACGGTCTACCGTTAGGTGCAAGAatat                                                                                                                      >  2:432003/1‑141 (MQ=12)
                       aactttAACAAGGATGTTATTACTGTAGCTGGTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAatat                                                                                                                      <  2:417190/138‑1 (MQ=255)
                        aaTTTAACATGGATTTTATCACTGTCGCCGTTGGCTACTATCTAAGATAGGCATTGAGCTAGCGTGATATATCTGAAATATTAAGAGAACGCGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                                                                                                                     <  1:26298/141‑1 (MQ=11)
                        aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                                                                                                                     <  1:692350/141‑1 (MQ=255)
                        aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                                                                                                                     >  1:466182/1‑141 (MQ=12)
                           ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGccc                                                                                                                  >  2:535092/1‑141 (MQ=255)
                           taaaaaaGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGccc                                                                                                                  <  1:585870/136‑1 (MQ=255)
                             aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                                                                                                                >  1:89550/1‑141 (MQ=255)
                              aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCGTTCAACGGTCTACCGTTGGGTTCAAGAATCTGCCCCaa                                                                                                               >  2:630725/1‑141 (MQ=12)
                              aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                               >  2:293595/1‑141 (MQ=255)
                                aaGGATGTTATCACTGTAGCTGTTGGCTACTCTCTAAGATATGAATTGAGTTATCGTGATATATCGGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTCCCGCTTGGATCAAGAATATGCCCCAAtt                                                                                                             >  2:6016/1‑141 (MQ=11)
                                 aGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAttt                                                                                                            >  1:486866/1‑141 (MQ=255)
                                  ggATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAtttt                                                                                                           >  2:266706/1‑141 (MQ=17)
                                  ggATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtttt                                                                                                           <  2:40493/141‑1 (MQ=255)
                                   gATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAATAATATGCCCCAATTTta                                                                                                          <  1:155921/141‑1 (MQ=17)
                                   gATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTta                                                                                                          <  1:348151/141‑1 (MQ=18)
                                   gATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTta                                                                                                          <  1:635766/141‑1 (MQ=255)
                                    aTGTTATCAGTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGGGAGATATCTTAAATATTAAGAGTAGGTGGTGTTAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCGCAATTTtat                                                                                                         <  2:148047/141‑1 (MQ=11)
                                    aTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATACGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                                                                                         <  1:450039/141‑1 (MQ=11)
                                       ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCACTTTTATATc                                                                                                      >  2:179533/1‑141 (MQ=255)
                                        tATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGGAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                                                                                     >  2:565502/1‑141 (MQ=25)
                                        tATCACTGTAGCCTTTGGCTACTATCTAAAATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                                                                                     <  1:390422/141‑1 (MQ=11)
                                           cACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAt                                                                                                  >  2:112966/1‑141 (MQ=25)
                                                 aGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                                                                            <  2:372100/141‑1 (MQ=25)
                                                 aGCCGTTGGTTACTATCTAAGATATGCATTGAGCTATNGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                                                                            <  2:759361/141‑1 (MQ=255)
                                                 aGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                                                                            <  2:200201/141‑1 (MQ=255)
                                                  gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCTTGATATATCTGGAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTACAAGAATATGCCCCAATTTTATTTCAATTTTTgaag                                                                                           >  1:769486/1‑141 (MQ=11)
                                                  gCCGTTGGCTACTAATGAAGATATGCATTTAGCTATCGTGATATACCTGAAATATTAAGAGAACGTGTTGTAAACGTTCATCATTCACCGATTTACCGTTGGGTTCAATAATATGCCCCAAGTTTATATCAAATTTGgaag                                                                                           <  2:275767/141‑1 (MQ=11)
                                                   ctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga                                                                                          >  2:669878/3‑141 (MQ=25)
                                                   ctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga                                                                                          >  2:442065/3‑141 (MQ=25)
                                                    tgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaagaa                                                                                         >  2:741460/2‑141 (MQ=25)
                                                    cGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaagaa                                                                                         >  2:100925/1‑141 (MQ=35)
                                                    cGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaataa                                                                                         >  2:167615/1‑141 (MQ=255)
                                                        ggttactaTCGAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                                                                                     <  2:450943/138‑1 (MQ=25)
                                                         gCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGc                                                                                    <  1:600923/141‑1 (MQ=255)
                                                         gCTAATATCTAAGATATGCATTGAGTTATCGTTATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGc                                                                                    <  2:208624/141‑1 (MQ=25)
                                                          ctactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                                                   <  2:90328/141‑1 (MQ=35)
                                                          ctactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                                                   >  1:73359/1‑141 (MQ=35)
                                                          ctactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                                                   >  1:363511/1‑141 (MQ=34)
                                                          ctactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                                                   >  2:338727/1‑141 (MQ=255)
                                                          ctactaTCTAAGATACGCATTGAGCTATCGTGATATATCTGAAGTATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGTTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAGAGCa                                                                                   <  1:556156/141‑1 (MQ=11)
                                                           tactaTCTAAGATATGCATTGAGTTATCGTGATAGATCTGAAATATTAAGGGACCGTTTTTGAAATTTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCTCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                  <  1:375967/141‑1 (MQ=11)
                                                           tactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                  <  1:248290/141‑1 (MQ=255)
                                                           tactaTCTAAGATATGCATGTGGCTATCTTGATATACCTGAAATATAAAGAGAACGTCGTGCAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                  <  2:475520/141‑1 (MQ=11)
                                                            actaTCTAAGAAATGCATTGAGTTAGCGTGATGTATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                                                                 <  2:577804/141‑1 (MQ=255)
                                                             ttaTTTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                                                                <  2:31179/140‑1 (MQ=25)
                                                             ctagttAAGGTATGCATTGAGCTATCGTGATATATCTGAAATATTGAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTTGGTTCAAGAATATGCCCCAATGTTATATCAAATTTGGAAGAAAAAGCATaa                                                                                <  2:158955/136‑1 (MQ=11)
                                                             ctaTCTAAGATATGCGTTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                                                                <  1:658266/141‑1 (MQ=255)
                                                             ctaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCCATTTTATATCAAATTTGGAAGAAAAAGCATaa                                                                                <  2:521788/141‑1 (MQ=21)
                                                              caTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                                                                              <  1:326141/140‑1 (MQ=255)
                                                               aTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa                                                                             >  1:677812/1‑141 (MQ=35)
                                                                tCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaa                                                                            <  1:507720/141‑1 (MQ=255)
                                                                tCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaa                                                                            <  1:628338/141‑1 (MQ=255)
                                                                 cTAAGATATGCATTGAGCTATCGTGAGATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaa                                                                           >  2:488840/1‑140 (MQ=11)
                                                                   aaGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagc                                                                         >  1:521894/1‑138 (MQ=11)
                                                                   aaGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGGTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGACGAAAAAGAATaaaaaagc                                                                         >  2:351791/1‑138 (MQ=11)
                                                                     gATATGCATTGAGTTACCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTaa                                                                       <  1:16827/141‑1 (MQ=255)
                                                                        atGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGCACGTGGTGTAAACGCTCGTCATTCAACGGTCAGCCGTTGGGTTCAAGAATGTGCCCCAATTTTATATCAAATTTGAAAGAAAAAGCATaaaacagcgtata                                                                    >  2:694365/1‑132 (MQ=11)
                                                                        atGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAGTAGGCTCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTa                                                                    >  1:649631/1‑141 (MQ=255)
                                                                                    aTCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTAt                                                        <  1:81575/141‑1 (MQ=38)
                                                                                              atCTGAAATATTAAGGGAACGTGGTCTAAACGGTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACg                                              <  1:110569/141‑1 (MQ=255)
                                                                                                        ttAAGGAAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAACAAGCATAACAAAGCTAATAACAAATGGCGTATTGATGATACATACATCaaa                                     >  2:653886/1‑141 (MQ=11)
                                                                                                             ggAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATaaaa                               <  1:128609/141‑1 (MQ=40)
                                                                                                                    ggtgTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAAt                        >  1:715932/1‑141 (MQ=40)
                                                                                                                              ttCATCATTCAACGGTCTACCGTTGGGTACAAGAATATGCCCCAATTTTATATCAAACTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTAttt              <  1:432003/141‑1 (MQ=255)
                                                                                                                                       cAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGc     >  1:620285/1‑141 (MQ=40)
                                                                                                                                            |                                                                                                                                           
GTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCAT  >  CP000730/76213‑76491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: