Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,889 G→A 21.2% intergenic (+947/‑187) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,8890GA21.2% 117.0 / 20.8 58intergenic (+947/‑187)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (27/18);  new base A (3/9);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 4.98e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01

GTATCAAAATATCAATACAGCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAA  >  CP000730/76750‑77029
                                                                                                                                           |                                                                                                                                            
gTATCAAAATATCCATACAGCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGc                                                                                                                                             <  1:323504/141‑1 (MQ=17)
gTATCAAAATATCAATACAGCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGc                                                                                                                                             <  1:721236/141‑1 (MQ=32)
gTATCAAAATATCAATACAGCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGc                                                                                                                                             >  2:251083/1‑141 (MQ=32)
 tATCAAAATACCAATACAGCAAATAATACTTTAAAAAGTATTAAACGTATTTACGCTCTATATAAAAAGAACCGCAAGTCTCTTCCGATCTACAGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCg                                                                                                                                            <  2:666615/141‑1 (MQ=11)
   taaaaaTATCAATACGGCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGTAGGTCTCTTCAGATCTACGGATTTTCCCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGaa                                                                                                                                          <  1:1271/139‑1 (MQ=11)
     caaggagcAATACAGCAACGAATACTTTAAAAGGTATTGAGTGTATTTACGCTCCATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAaca                                                                                                                                        <  2:464756/134‑1 (MQ=1)
     aaaaTATCAATACAGCAAACAATACTTTAAAAGGTATTGAATTTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACTAAATTAGCATCATGCTAGCAAGTTAAGCGAaca                                                                                                                                        <  2:652561/141‑1 (MQ=12)
      aaaTATCAATACAGCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAacac                                                                                                                                       >  1:74178/1‑141 (MQ=32)
       agtatcaaccgaGCAAGGAATACTTTAAAAGGTATTGAGTGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGACCACt                                                                                                                                      >  2:764315/12‑141 (MQ=11)
       aggatCAATACAGCAAAGAATACGTGAAAAGGTATTGAGTGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACt                                                                                                                                      <  2:550929/138‑1 (MQ=1)
        atatCAATACAGCAAATAATACTTTAAAAGCTATTGAGTGTATTTACGCTCTATATAAAAAGAACCGCATGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTg                                                                                                                                     <  2:512667/141‑1 (MQ=11)
               aacggCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGTAGGTCTCTTCAGATCTACGGATTTTCCCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGAt                                                                                                                              <  2:739938/137‑1 (MQ=0)
                        gactACTTTAAAAGGTATTGAATGTATTTACTCTCTATATAAAACGAACCGTAGGTCTCTTCAGATCTACGGATTTTCCCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTg                                                                                                                     <  1:151351/138‑1 (MQ=1)
                              ttgaaaaGGTATTGAAGTTAATTACGCTCTATATAAAAAGAACCGTAGGTCTCTTCAGATCTACGGATTTTCCCCATGGCACGAAATTAGCATCATGCTAGCAAGTTAAAGGATGCTTTACATGATATATTAGTGATTAGc                                                                                                               <  2:363012/138‑1 (MQ=1)
                                      gtagtgatggTATTTAGGTTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATAtttt                                                                                                       <  1:355510/132‑1 (MQ=1)
                                      gcatagagtGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGACCTACGGATTTTCGCCATGCCACCAAATTAGAATCATGCTAGCAAGTTATACGAACACTGACATGATATATTAGTGATTAGCTATAtttt                                                                                                       <  1:360048/133‑1 (MQ=1)
                                           gagtGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTGATTAGCTATAttttttact                                                                                                  >  1:427155/4‑138 (MQ=1)
                                             atgttgttaccgtctATATAAAGAGAACCGTAGTTCTCTTCAGAGCTTCTGATGTTCCCCATGCCATGAAATTAGCATCATGCTAGCAAGTTAACCGAACACGGACAGGATATATTAGTGATTAGCTATATTGTTTACttt                                                                                               <  2:215336/129‑1 (MQ=0)
                                                      cgatctATATAAAAAGAACCGTAGTTCTCTTCAGATCTACGGATATTCCCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGCCATGATAAATTAGTGATTAGCTATATTTTCTACTTTGCAACAGaa                                                                                      >  2:267967/4‑141 (MQ=1)
                                                         cttacataAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTGATTAGCTATATTTTTTACTTTGCAACAGAAcca                                                                                   <  2:120364/136‑2 (MQ=1)
                                                              ataAAAAGACCCGCCGGTCTCTTCAGATCTACGGATTTTCGCCAGGCCACGAAATTAGACTCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTATCTATATTTTTTTACTTTGCAACAGAACCTATaa                                                                               <  2:404701/141‑1 (MQ=11)
                                                                  aatgAACCGCAGGTCTTCTCAGATCTACGGATTTTCGCCATGCCACGGAGTTAGAAGCATGCTAGCAAGTCAAGCGAACACTGACATGATACATTACTGGTTAGCGATAGTTTTTTACTTTGCAACAGAACCTATaaaaaa                                                                           <  2:201125/138‑1 (MQ=11)
                                                                  aaaGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACTAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATaaaaaa                                                                           <  1:19388/141‑1 (MQ=25)
                                                                    aGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCAAGCTAGCAAGTGAAACCAACAGTGACACGATAAATTAGCGTATAGCTATATTTTTTACTTTGCAACAGAAccatcatttgtatt                                                                        >  2:756688/1‑129 (MQ=11)
                                                                        ccGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATggg                                                                     >  1:56983/1‑141 (MQ=40)
                                                                         cGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGc                                                                    >  1:645646/1‑141 (MQ=255)
                                                                         cGCAGGTCTCTTCAGATCTACGGATTTTCACCATGCCACGAAATTAGCGTCATGCGAGCCAGTTAGGCGAACACTGGCATGATAAATTAGAGGTTAGCTAGATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGc                                                                    >  2:197776/1‑141 (MQ=14)
                                                                          gCAGGTCTCTTCAGATTTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTAATTTGCAACAGAACCTATAAAAAACATGGGCt                                                                   <  1:230711/141‑1 (MQ=255)
                                                                          gCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCt                                                                   >  2:27564/1‑141 (MQ=255)
                                                                           cAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCAAAATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCtt                                                                  >  1:346401/1‑141 (MQ=255)
                                                                            aGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTa                                                                 >  1:400303/1‑141 (MQ=255)
                                                                             ggTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTaa                                                                >  1:419416/1‑141 (MQ=255)
                                                                              gTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTTTAACAGAACCTATAAAAAACATGGGCTTaaa                                                               >  2:638228/1‑141 (MQ=255)
                                                                               tctcTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAg                                                              <  1:711075/141‑1 (MQ=255)
                                                                                ctctTCAGATCTACGGATTTTCGCCATGCCAGGAAATTAGCATCATGCTAGCAAGTTACGCGCACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAAACTATCAAAAACAAGGGATTAAAgt                                                             >  2:228969/1‑140 (MQ=14)
                                                                                ctctTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAgg                                                             >  1:234259/1‑141 (MQ=255)
                                                                                ctctTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAgg                                                             <  1:652973/141‑1 (MQ=255)
                                                                                         tCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTTCAACAGAACCTATAAAAAACATTGGCTTAAAGGGTTTAATta                                                    >  1:71137/1‑141 (MQ=255)
                                                                                            aCGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCGGCGTTGGGTTAGTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTtatat                                                 >  1:774454/1‑141 (MQ=18)
                                                                                            aCGGATTTTCGCCATGCCACCAAATTAGCATCATGCTAGCAAGTCAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTGTATTACTTTGCAACAGAACCTCTAAAAAACATGGGCTTAAAGGGTTAATTtatat                                                 >  2:433848/1‑141 (MQ=17)
                                                                                                   ttCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTa                                          >  2:52033/1‑141 (MQ=255)
                                                                                                   ttCGCCATGCCACGAAATTAGCACCATGCCAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTa                                          <  2:472648/141‑1 (MQ=255)
                                                                                                     cgtcatctcACGAAAGTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACt                                        <  2:256399/133‑1 (MQ=255)
                                                                                                     cGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAGTTAATATAGTATTACt                                        >  1:491405/1‑141 (MQ=255)
                                                                                                     cGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACt                                        <  1:111207/141‑1 (MQ=255)
                                                                                                     cGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACt                                        >  1:193213/1‑141 (MQ=255)
                                                                                                      gCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGATTAAAGGGTTCATTTATATAGTactactc                                       >  2:207555/1‑140 (MQ=255)
                                                                                                       ccATGCCACGAAATTAGCATCTTGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGc                                      <  1:341260/141‑1 (MQ=255)
                                                                                                       ccATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGc                                      >  1:515379/1‑141 (MQ=255)
                                                                                                               cGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATTGTATTACttaaacacttg                              >  2:415785/1‑131 (MQ=255)
                                                                                                               cGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACAtta                              >  1:643831/1‑140 (MQ=255)
                                                                                                                        catcatGCTAGCAAGTTAAGCGAACACTGACAGGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTc                     <  1:294769/141‑1 (MQ=255)
                                                                                                                         atcatGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCa                    <  1:777177/141‑1 (MQ=255)
                                                                                                                                    aaGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTTCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAATCTAgggg         >  2:149600/1‑141 (MQ=255)
                                                                                                                                       ttAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTAt      >  1:629476/1‑141 (MQ=255)
                                                                                                                                       ttAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTAGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTAt      >  1:742730/1‑141 (MQ=255)
                                                                                                                                       gtAAGCGAACACTGACATTATAAATTAGTGGTTAGCTATATTTTTATAAATTGCAACAGAAAATATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGTTCAAAAATTaaaaaaaa      <  2:74178/140‑8 (MQ=25)
                                                                                                                                          aGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaa   >  1:637626/1‑141 (MQ=255)
                                                                                                                                           gCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaaa  <  1:160100/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
GTATCAAAATATCAATACAGCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAA  >  CP000730/76750‑77029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: