Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,915 G→A 12.8% intergenic (+973/‑161) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,9150GA12.8% 136.2 / 5.6 53intergenic (+973/‑161)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (28/17);  new base A (2/5);  total (31/22)
Fisher's exact test for biased strand distribution p-value = 1.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTT  >  CP000730/76780‑77050
                                                                                                                                       |                                                                                                                                       
ttgaaaaGGTATTGAAGTTAATTACGCTCTATATAAAAAGAACCGTAGGTCTCTTCAGATCTACGGATTTTCCCCATGGCACGAAATTAGCATCATGCTAGCAAGTTAAAGGATGCTTTACATGATATATTAGTGATTAGc                                                                                                                                    <  2:363012/138‑1 (MQ=1)
        gtagtgatggTATTTAGGTTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATAtttt                                                                                                                            <  1:355510/132‑1 (MQ=1)
        gcatagagtGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGACCTACGGATTTTCGCCATGCCACCAAATTAGAATCATGCTAGCAAGTTATACGAACACTGACATGATATATTAGTGATTAGCTATAtttt                                                                                                                            <  1:360048/133‑1 (MQ=1)
             gagtGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTGATTAGCTATAttttttact                                                                                                                       >  1:427155/4‑138 (MQ=1)
               atgttgttaccgtctATATAAAGAGAACCGTAGTTCTCTTCAGAGCTTCTGATGTTCCCCATGCCATGAAATTAGCATCATGCTAGCAAGTTAACCGAACACGGACAGGATATATTAGTGATTAGCTATATTGTTTACttt                                                                                                                    <  2:215336/129‑1 (MQ=0)
                        cgatctATATAAAAAGAACCGTAGTTCTCTTCAGATCTACGGATATTCCCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGCCATGATAAATTAGTGATTAGCTATATTTTCTACTTTGCAACAGaa                                                                                                           >  2:267967/4‑141 (MQ=1)
                           cttacataAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTGATTAGCTATATTTTTTACTTTGCAACAGAAcca                                                                                                        <  2:120364/136‑2 (MQ=1)
                                ataAAAAGACCCGCCGGTCTCTTCAGATCTACGGATTTTCGCCAGGCCACGAAATTAGACTCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTATCTATATTTTTTTACTTTGCAACAGAACCTATaa                                                                                                    <  2:404701/141‑1 (MQ=11)
                                    aatgAACCGCAGGTCTTCTCAGATCTACGGATTTTCGCCATGCCACGGAGTTAGAAGCATGCTAGCAAGTCAAGCGAACACTGACATGATACATTACTGGTTAGCGATAGTTTTTTACTTTGCAACAGAACCTATaaaaaa                                                                                                <  2:201125/138‑1 (MQ=11)
                                    aaaGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACTAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATaaaaaa                                                                                                <  1:19388/141‑1 (MQ=25)
                                      aGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCAAGCTAGCAAGTGAAACCAACAGTGACACGATAAATTAGCGTATAGCTATATTTTTTACTTTGCAACAGAAccatcatttgtatt                                                                                             >  2:756688/1‑129 (MQ=11)
                                          ccGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATggg                                                                                          >  1:56983/1‑141 (MQ=40)
                                           cGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGc                                                                                         >  1:645646/1‑141 (MQ=255)
                                           cGCAGGTCTCTTCAGATCTACGGATTTTCACCATGCCACGAAATTAGCGTCATGCGAGCCAGTTAGGCGAACACTGGCATGATAAATTAGAGGTTAGCTAGATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGc                                                                                         >  2:197776/1‑141 (MQ=14)
                                            gCAGGTCTCTTCAGATTTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTAATTTGCAACAGAACCTATAAAAAACATGGGCt                                                                                        <  1:230711/141‑1 (MQ=255)
                                            gCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCt                                                                                        >  2:27564/1‑141 (MQ=255)
                                             cAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCAAAATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCtt                                                                                       >  1:346401/1‑141 (MQ=255)
                                              aGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTa                                                                                      >  1:400303/1‑141 (MQ=255)
                                               ggTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTaa                                                                                     >  1:419416/1‑141 (MQ=255)
                                                gTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTTTAACAGAACCTATAAAAAACATGGGCTTaaa                                                                                    >  2:638228/1‑141 (MQ=255)
                                                 tctcTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAg                                                                                   <  1:711075/141‑1 (MQ=255)
                                                  ctctTCAGATCTACGGATTTTCGCCATGCCAGGAAATTAGCATCATGCTAGCAAGTTACGCGCACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAAACTATCAAAAACAAGGGATTAAAgt                                                                                  >  2:228969/1‑140 (MQ=14)
                                                  ctctTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAgg                                                                                  <  1:652973/141‑1 (MQ=255)
                                                  ctctTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAgg                                                                                  >  1:234259/1‑141 (MQ=255)
                                                           tCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTTCAACAGAACCTATAAAAAACATTGGCTTAAAGGGTTTAATta                                                                         >  1:71137/1‑141 (MQ=255)
                                                              aCGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCGGCGTTGGGTTAGTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTtatat                                                                      >  1:774454/1‑141 (MQ=18)
                                                              aCGGATTTTCGCCATGCCACCAAATTAGCATCATGCTAGCAAGTCAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTGTATTACTTTGCAACAGAACCTCTAAAAAACATGGGCTTAAAGGGTTAATTtatat                                                                      >  2:433848/1‑141 (MQ=17)
                                                                     ttCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTa                                                               >  2:52033/1‑141 (MQ=255)
                                                                     ttCGCCATGCCACGAAATTAGCACCATGCCAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTa                                                               <  2:472648/141‑1 (MQ=255)
                                                                       cgtcatctcACGAAAGTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACt                                                             <  2:256399/133‑1 (MQ=255)
                                                                       cGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAGTTAATATAGTATTACt                                                             >  1:491405/1‑141 (MQ=255)
                                                                       cGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACt                                                             <  1:111207/141‑1 (MQ=255)
                                                                       cGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACt                                                             >  1:193213/1‑141 (MQ=255)
                                                                        gCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGATTAAAGGGTTCATTTATATAGTactactc                                                            >  2:207555/1‑140 (MQ=255)
                                                                         ccATGCCACGAAATTAGCATCTTGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGc                                                           <  1:341260/141‑1 (MQ=255)
                                                                         ccATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGc                                                           >  1:515379/1‑141 (MQ=255)
                                                                                 cGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATTGTATTACttaaacacttg                                                   >  2:415785/1‑131 (MQ=255)
                                                                                 cGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACAtta                                                   >  1:643831/1‑140 (MQ=255)
                                                                                          catcatGCTAGCAAGTTAAGCGAACACTGACAGGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTc                                          <  1:294769/141‑1 (MQ=255)
                                                                                           atcatGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCa                                         <  1:777177/141‑1 (MQ=255)
                                                                                                      aaGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTTCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAATCTAgggg                              >  2:149600/1‑141 (MQ=255)
                                                                                                         ttAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTAt                           >  1:629476/1‑141 (MQ=255)
                                                                                                         ttAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTAGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTAt                           >  1:742730/1‑141 (MQ=255)
                                                                                                         gtAAGCGAACACTGACATTATAAATTAGTGGTTAGCTATATTTTTATAAATTGCAACAGAAAATATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGTTCAAAAATTaaaaaaaa                           <  2:74178/140‑8 (MQ=25)
                                                                                                            aGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaa                        >  1:637626/1‑141 (MQ=255)
                                                                                                             gCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaaa                       <  1:160100/141‑1 (MQ=255)
                                                                                                              cGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGCTCAAAAATTAGGGGTATTaaaa                      >  2:639313/1‑141 (MQ=255)
                                                                                                                 agcgtGACATGATAAATGAGTGGTTAGCTATCTTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATCACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaaaaaaa                   <  2:131008/137‑1 (MQ=255)
                                                                                                                     tGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGAGCTTAAAGGGTTACTTTATATAGTATTACTGCAAACATTGATTTGGGTCTAAAATTACGGGTATTAAAAAattgaa               >  2:527318/1‑141 (MQ=37)
                                                                                                                      gACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCAATAAAAGACATGGGCTTAAAGGGTCAATACCAAAAACGGCACGCCAAACAGGGATTGGGGTCAAAAAGTAGGGGGATTAAAAAaatgaaa              >  2:555750/1‑140 (MQ=12)
                                                                                                                            aTAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCa        >  1:681837/1‑141 (MQ=255)
                                                                                                                             tAAATTAGTGATTAGCTAGATCTTTTTACTCTGTAACAGAACCTATAAAAAATATGGGCTTAAAGGGTTAATTTATATAGTCTTCGCGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCAt       <  1:458056/141‑1 (MQ=255)
                                                                                                                                  tAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAAttt  <  1:743969/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TTTAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTT  >  CP000730/76780‑77050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: