Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 110,660 T→A 19.9% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730110,6600TA19.9% 162.2 / 37.1 75I173I (ATT→ATAUSA300HOU_0109staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (22/38);  new base A (8/7);  total (30/45)
Fisher's exact test for biased strand distribution p-value = 2.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01

CGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGG  >  CP000730/110521‑110794
                                                                                                                                           |                                                                                                                                      
cGGTTAAAATGGTCGATAATCAAATCAATCCAAAAAAAGAAATTAAAGATGAAACACTAAAAGCACAGATCGAAAACGTTAAGTTCTTTGTTCAATATGGCCGCTTTAAAAATTTGAAAGATTATAAAGAAGCAGATAttt                                                                                                                                       >  2:296554/1‑141 (MQ=17)
    tAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCata                                                                                                                                   <  1:42729/141‑1 (MQ=255)
      aaaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTAGAAATATTATAAAGACGGAGATATTTCAtata                                                                                                                                 >  2:20880/1‑141 (MQ=255)
        aTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAACACGGAGATATTTCATATAAt                                                                                                                               >  1:161017/1‑141 (MQ=255)
            tCGATAATAAAAAAAATCCAACAAAAAAAATTAAAAATAAAAAAATAAAAAAGAAACCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCaga                                                                                                                          <  2:700231/141‑1 (MQ=17)
               ataataAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAgg                                                                                                                        >  2:717801/1‑141 (MQ=255)
               ataataAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAgg                                                                                                                        <  1:225858/141‑1 (MQ=255)
                   taaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                    >  1:418603/1‑141 (MQ=255)
                    aaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAATAGAAATGGAAAACTTTAAGTTATTTGTTCAATATGGCGACTTTAAAAATTTGAAACATTTTTAAAACGTTGATAATTTATATAATTAATAGGTGTCg                                                                                                                   >  2:63356/1‑141 (MQ=17)
                     aaaTCATTCCAACAAAAGGAATTAAAGAAGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                                  <  1:363510/141‑1 (MQ=255)
                     aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                                  <  1:767929/141‑1 (MQ=255)
                          ataccAACAAAAGAAATTAAAGATGAAAAAAAAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTAt                                                                                                             <  2:171804/138‑1 (MQ=255)
                          aTTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGTGATATTTCATATAATCCAGAGGTGCCGAGTTAt                                                                                                             >  1:536991/1‑141 (MQ=255)
                          aTTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTAt                                                                                                             <  2:103284/141‑1 (MQ=255)
                          aTTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTAt                                                                                                             <  2:373302/141‑1 (MQ=255)
                            tCCAACAAAAGAAATTAAAGATGCAAAAATAACAAAAGAAATCGAAAACTTTAAGTTCTTTGGTAAATATCGCGACTCTAAAAATCTGAAAAATTATAAAGACGGAGATATTTCATATAATCCCGAGGTGCAGAGAGATTc                                                                                                           >  2:200419/1‑141 (MQ=17)
                            tCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTGCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGAAGATATTTCATATAATCAAGAGGTGCAGAGCTATTc                                                                                                           >  2:384269/1‑141 (MQ=17)
                                 caaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTa                                                                                                      >  1:153345/1‑141 (MQ=255)
                                  aaaaaaaaATAAAGAATAAAAAAAAAAAAAAGCAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTaa                                                                                                     <  2:700774/141‑1 (MQ=17)
                                  aaaaTAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTaa                                                                                                     <  2:388715/141‑1 (MQ=255)
                                        aatgacaGATGAAATAATTAAATATAGAGTCGAAAACTTTAAGTACTATAAGCAAGATGGCGACGTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCCAGTTATTCGGCTGAATATCa                                                                                               <  1:494092/135‑1 (MQ=12)
                                             aaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                          <  2:189977/141‑1 (MQ=255)
                                               tcgcaaaaaataaaaaatGAAATCAAACACTTTAAGTTCTTTGTTCACTTTGGTGACTTTAAAAACTGGAAAAATTACAAAGAAGGAGATATTTCATATAAGCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAcc                                                                                        <  1:209630/137‑2 (MQ=14)
                                               gATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                        <  1:180597/141‑1 (MQ=255)
                                               gATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                        <  1:759418/141‑1 (MQ=255)
                                                aTGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                                       <  2:370991/141‑1 (MQ=255)
                                                aTGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                                       <  1:240465/141‑1 (MQ=255)
                                                 tGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGTATTTTAATTCGGCTAAATATCAATTAACTaa                                                                                      <  1:781492/141‑1 (MQ=18)
                                                     aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                  >  1:424713/1‑141 (MQ=255)
                                                       aaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTAGTCGCGAATATTTCAATTAACTAAtgatga                                                                                <  2:364334/141‑1 (MQ=17)
                                                           aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                            <  1:114454/141‑1 (MQ=255)
                                                            aaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAGtata                                                                           <  2:547452/141‑1 (MQ=255)
                                                                  aaaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCATATGTGCCGTGTTGTTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                     <  2:48024/141‑1 (MQ=255)
                                                                  aaaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                     <  2:124257/141‑1 (MQ=255)
                                                                        aaaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATAATAAAGACGGCGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATAGCCATTAACTAATGAAGACtagcatcgacaaccat                                                               >  1:333216/1‑127 (MQ=17)
                                                                           aCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAAAAttcc                                                            >  2:254266/1‑139 (MQ=255)
                                                                            ctctAAGTTCTTTGGTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACg                                                           <  1:772953/138‑1 (MQ=255)
                                                                             tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                          <  1:646219/141‑1 (MQ=255)
                                                                               tAAGTTCCTTGTTCAATATCGCCACTTTAAAAATTTGAAAAATTAGAAAGACGGACATACTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATCAACTAATGATGATTATAATGTAACACAATTACGCaa                                                        <  2:295029/141‑1 (MQ=17)
                                                                                     cTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgata                                                  >  1:633971/1‑141 (MQ=255)
                                                                                        tcttCAAGATGGCGACGTTAAAAATTTGAAAAATTAGAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatga                                               <  1:348745/139‑1 (MQ=255)
                                                                                        tGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatga                                               <  1:248404/141‑1 (MQ=255)
                                                                                            cAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATAACAATTAACTAATGATGATTATAATGTCAACCAATTACGACAAAGATATTGtata                                           >  1:653010/1‑141 (MQ=17)
                                                                                                atGGCGACTTTAAAAATTTGGAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcga                                       <  2:290749/141‑1 (MQ=255)
                                                                                                  tgCGACTTTAAAAAGTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGGGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATTATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacg                                     <  2:72685/140‑1 (MQ=255)
                                                                                                  ggCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacg                                     >  2:431046/1‑141 (MQ=255)
                                                                                                       ttttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAACCAATTACGCAAAAGATATGATATACCGACGAGTaa                                <  2:71894/140‑1 (MQ=11)
                                                                                                         ttAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGCTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAg                              <  2:520694/141‑1 (MQ=255)
                                                                                                          taaagatgtaaggaaTTACAAAGACGAACATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                             <  1:177459/128‑1 (MQ=14)
                                                                                                          tAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAga                             >  1:632078/1‑140 (MQ=255)
                                                                                                           aaagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                            >  1:663049/13‑141 (MQ=11)
                                                                                                            aagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGTTc                           >  2:119865/12‑141 (MQ=11)
                                                                                                            aagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTc                           >  1:618771/12‑141 (MQ=11)
                                                                                                            aagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTc                           >  1:351557/12‑141 (MQ=11)
                                                                                                            aaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTc                           >  2:3169/1‑141 (MQ=255)
                                                                                                            aaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAACTc                           >  2:613824/1‑141 (MQ=255)
                                                                                                              gatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGCCGAGTAAAGCTCCa                         >  2:122263/10‑141 (MQ=11)
                                                                                                               aTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaa                        <  1:708963/141‑1 (MQ=255)
                                                                                                                 ttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGCCGAGTAAAGCTCCAAAg                      >  1:698602/7‑141 (MQ=14)
                                                                                                                   gAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                    >  1:55546/1‑141 (MQ=255)
                                                                                                                     aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt                  <  1:434420/141‑1 (MQ=255)
                                                                                                                      aaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAtt                 >  1:594725/1‑141 (MQ=255)
                                                                                                                         ttATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGtt              <  1:714464/141‑1 (MQ=255)
                                                                                                                         ttATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGtt              >  1:101860/1‑141 (MQ=255)
                                                                                                                           ataAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa            <  1:310939/141‑1 (MQ=255)
                                                                                                                            taAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa           <  2:749630/141‑1 (MQ=255)
                                                                                                                            aaaaGAGGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa           <  1:244480/140‑1 (MQ=255)
                                                                                                                             aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAGTGTTaaaa          >  2:213675/1‑141 (MQ=255)
                                                                                                                             aaaGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaaa          >  2:776121/1‑141 (MQ=255)
                                                                                                                              aaGACGGGGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAg         <  2:418603/141‑1 (MQ=255)
                                                                                                                               agttggAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAgg        <  1:27572/137‑1 (MQ=255)
                                                                                                                                gACGGAGATATTTCATATANTCCAGAGGTGCCGTGTTATTCGGCTAAATNTCAAGTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt       <  1:654264/141‑1 (MQ=255)
                                                                                                                                gACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt       <  1:662191/141‑1 (MQ=255)
                                                                                                                                   ggAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTcg    >  1:417910/1‑140 (MQ=255)
                                                                                                                                     agaTATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCGAAAGATATGATATACCGACGAGTAAAGCTCAAAAGTTATTGTTAAAAGGTTCAgg  <  1:503454/141‑1 (MQ=255)
                                                                                                                                     agaTATATCAAATAATCCAGAGGGGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAgg  <  2:136200/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                      
CGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGG  >  CP000730/110521‑110794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: