Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 462,218 A→T 11.7% K329I (AAA→ATA)  USA300HOU_0436 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730462,2180AT11.7% 222.5 / 8.7 85K329I (AAA→ATA) USA300HOU_0436hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (30/43);  new base T (4/7);  total (35/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAAAATGATGAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGACTAAATTCGCAAAT  >  CP000730/462078‑462357
                                                                                                                                             |                                                                                                                                            
gAAAAATGATGAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGaaaa                                                                                                                                              >  1:167283/1‑141 (MQ=255)
 aaaaaTGATGAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTACTTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGaaaaa                                                                                                                                             >  2:378186/1‑141 (MQ=255)
 aaaaaTGATGAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGaaaaa                                                                                                                                             <  1:668750/141‑1 (MQ=255)
    aaTGATGAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAaaaagta                                                                                                                                          >  1:652837/1‑141 (MQ=255)
     atgatgAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAaaaagtaa                                                                                                                                         <  1:703632/141‑1 (MQ=255)
     atgatgAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAaaaagtaa                                                                                                                                         <  1:339125/141‑1 (MQ=255)
     atgatgAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGGGGAaaaagtaa                                                                                                                                         >  1:33275/1‑141 (MQ=255)
       gatgaGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACAAAAGTGGAaatagtaaaa                                                                                                                                       >  2:468336/1‑141 (MQ=255)
       gatgaGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGGTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAaaaagtaaaa                                                                                                                                       >  2:218424/1‑141 (MQ=255)
          gagaTCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAaaagtaaaagta                                                                                                                                    >  1:154054/1‑141 (MQ=255)
          gagaTCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAaaagtaaaagta                                                                                                                                    >  1:648286/1‑141 (MQ=255)
             atgtaattaaactctGCACCTGAGGAAGAAGACGAAGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTg                                                                                                                                 <  2:609373/127‑1 (MQ=255)
              tctcATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGa                                                                                                                                <  1:485455/141‑1 (MQ=255)
               ctcATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAGAGTAAAAGTATTGaa                                                                                                                               >  1:492782/1‑141 (MQ=255)
               ctcATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGaa                                                                                                                               >  1:44738/1‑141 (MQ=255)
               ctcATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGGAACGAGTTTAGAAGATATTGATAGAGCAGAAACTAAATATGACCATACTAAAGTGGGAAAGGAAAAAGTGTTaaa                                                                                                                               >  1:357212/1‑141 (MQ=255)
                tcATTAACATTTGCACCTGATGACGAAGATGAGGAATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGaaa                                                                                                                              <  2:252488/141‑1 (MQ=255)
                   ttAACATTTGCACCTGATGACGAAGACGAGCCATCGAACTCACGAGTTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGAt                                                                                                                           <  1:216423/141‑1 (MQ=255)
                     aaCATTTGCACCTGATGACGAAGATGAGGCATTGAAGTGGTGAATGTGCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAGTAGTAAAAGTATTGAAAGATGt                                                                                                                         >  1:417839/1‑141 (MQ=255)
                       cATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTa                                                                                                                       >  1:636997/1‑141 (MQ=255)
                       cATTTGCACCTGATGACGAAGATAAGGTATTGAATGCAGTAACTTCCCAAACTCAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCCTACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTa                                                                                                                       <  1:694166/141‑1 (MQ=255)
                       aaTTTGCAACTGATGACGAAGATGAAGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGATCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTa                                                                                                                       <  1:563176/140‑1 (MQ=255)
                        aTTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTaa                                                                                                                      <  1:198590/141‑1 (MQ=255)
                              aCCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAagaaga                                                                                                                >  2:4419/1‑141 (MQ=255)
                                cTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGAtt                                                                                                              >  1:269853/1‑141 (MQ=255)
                                       cGAAGATGAGACATTGAAGTCATTAATTTTCAAAACGAAGGTCACCAGCGTATTAGAGATTTTTAAAGCAGAAACGAAATATGACCATACTAAAGTGGAAAAGGTAAAAGAATTGAAAGACGTTAAAGAAGATTTACAtat                                                                                                       >  2:556398/1‑139 (MQ=255)
                                          aGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAATAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGa                                                                                                    <  1:367611/141‑1 (MQ=255)
                                           gATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGAt                                                                                                   <  1:269439/141‑1 (MQ=255)
                                                   aTTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTa                                                                                           <  1:763971/141‑1 (MQ=255)
                                                   aTTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTa                                                                                           <  1:67393/141‑1 (MQ=255)
                                                   aTTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTa                                                                                           <  1:451788/141‑1 (MQ=255)
                                                   aTTGAAGTCATTAATTTTCAAAACGAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTa                                                                                           <  1:256434/141‑1 (MQ=255)
                                                      ggaGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACgg                                                                                        <  2:369236/139‑1 (MQ=255)
                                                      aaaGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACgg                                                                                        <  2:668903/140‑1 (MQ=255)
                                                         gTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACAAAATACGACCATACTAAAGGGGAAAAAGTAAAAGTAGTGAAAGATGTTAAAGAAGATTCACATGGAGACGAAATTGACAGacg                                                                                     >  1:130210/1‑139 (MQ=255)
                                                                  tttCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAAAAGACACTAAGTAAGAGCATACTAAAGGGGAAAAAGTAAAAGAATTGAAAGATGTAAAAGAAGATTTACATGTACATGAAATTTACGGAACCTTATATcc                                                                            >  2:360918/1‑140 (MQ=255)
                                                                   ttCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATAATAAAGTGGAAATAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCAt                                                                           >  1:697793/1‑141 (MQ=255)
                                                                    tCAAAACTAAGTTAACTAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATa                                                                          <  1:422121/141‑1 (MQ=255)
                                                                    tCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACCGAAGCTTATATCATa                                                                          <  1:586894/141‑1 (MQ=255)
                                                                    tCAAAACAAAGGAAACAAGTTTAGAAGATATTGATAAAGCAGAAACTAAATAAGAAAAAAAAAAAGGGGAAAAAGAAAAAGTATTGAAAGATGTTAAAGAAGCTTTACATGTAGGTGAGATTTACGGTAGTTtaaaaaaaa                                                                          <  2:37327/141‑8 (MQ=255)
                                                                       aaaCTAAGGTAACGAGTTGAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGCATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAg                                                                       <  2:16324/141‑1 (MQ=255)
                                                                       aaaCTAAGGTAACGAGGTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAg                                                                       <  1:690873/141‑1 (MQ=255)
                                                                           ttaGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATAGGACCATACGAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTAGGGAAGCTTATATCATACAGaaaa                                                                  >  2:526253/3‑141 (MQ=255)
                                                                           tcaGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTCTGGTTGCTTATACCATACTGatac                                                                   <  2:782270/139‑4 (MQ=255)
                                                                            aaGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGaaaaa                                                                 <  2:652837/141‑1 (MQ=255)
                                                                              ggTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAaaagg                                                               >  1:106837/1‑141 (MQ=255)
                                                                              ggTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAaaagg                                                               >  1:215187/1‑141 (MQ=255)
                                                                                gaaCGAGGTTAGAAGATAATGATAAAGCAGAAACTAAATATGACCATACTAAAGGGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAACTTACGGAAGCTTATATAATACAGAAaaaggta                                                             <  1:292605/140‑1 (MQ=255)
                                                                                  aCGAGTTTAGAAGATATTGATTAAGCACAAACTGAATATGCCCCCCCCCCAAGTGAAGAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAaaaggtaaa                                                           <  1:179239/141‑1 (MQ=255)
                                                                                  aCGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATATACGGAAGCTTATATCATACAGAAaaaggtaaa                                                           >  2:440769/1‑141 (MQ=255)
                                                                                  aCGAGGTTAGAAGATATTGATAAAGCAGGAACTAAATATGACCATACTAAAGTGGAAAAAGTAGAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAAATTACGGAAGCTAATATGATACAGAAaaaggtaaa                                                           >  2:298530/1‑141 (MQ=255)
                                                                                     aGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAaaaaggaaaagga                                                        >  2:98745/1‑140 (MQ=255)
                                                                                     aGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAaaaggtaaaggt                                                        <  1:723831/141‑1 (MQ=255)
                                                                                       tttAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTAt                                                      >  2:67519/1‑141 (MQ=255)
                                                                                        ttAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTAtt                                                     >  2:647870/1‑141 (MQ=255)
                                                                                        ttAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTAtt                                                     >  2:574255/1‑141 (MQ=255)
                                                                                         tAGAAGATATTGATAAAGGAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTc                                                    <  2:131659/141‑1 (MQ=255)
                                                                                         tAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTc                                                    <  2:695319/141‑1 (MQ=255)
                                                                                           gaagaTATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCtt                                                  <  1:4419/141‑1 (MQ=255)
                                                                                             agaTATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGa                                                >  1:701869/1‑141 (MQ=255)
                                                                                             agaTATTGATAAAGCAGAAACCAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGa                                                <  1:68677/141‑1 (MQ=255)
                                                                                              aaaaaagaaaaaGCAGAAACTAAATATGAACATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAACTTTACGGAAGCTTAAAAAAAAAAAAAAAAGGTAAAGGTATTCTTGAt                                               <  2:598665/132‑1 (MQ=255)
                                                                                               atatTGATAAAGCAGAAACTAAATAAGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATa                                              <  1:232957/141‑1 (MQ=255)
                                                                                               atatTGAAAAAGCAGAAACGAAATATGAACAAAAAAAAAAAAAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATa                                              <  2:568505/141‑1 (MQ=255)
                                                                                                tatTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAATAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATCCTTGATaa                                             <  1:424149/141‑1 (MQ=255)
                                                                                                 atTGATAAAGCAGAACCTACATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGACAAAGGTAAAGGTATTCTTGATaaa                                            <  2:231532/141‑1 (MQ=255)
                                                                                                   tgatttagtaaaaaCTAAAGATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGCAGCACGTGCACTTTATAAGAAGGTAAAGGTATTGTTGATAaaga                                          >  2:754572/12‑141 (MQ=255)
                                                                                                   tGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGATAAAGAAGATTTACATGTAGATGAAATTTACGGAAGATGAAATCATACAGAACAAGGGAAAGGTATTGTTGATAaaga                                          >  2:122086/1‑141 (MQ=255)
                                                                                                     aTAAAGCAGAAACTAAATATTACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAaagaag                                        <  1:378186/141‑1 (MQ=255)
                                                                                                       aaaGCAGTAAACAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATCAGCACCTGGGCAAAGGTATTCTTGATAAAGAAGGt                                      <  1:189026/141‑1 (MQ=255)
                                                                                                          gCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACt                                   >  2:108986/1‑141 (MQ=255)
                                                                                                            aGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTaa                                 >  2:668938/1‑141 (MQ=255)
                                                                                                             gAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTaaa                                >  2:523813/1‑141 (MQ=255)
                                                                                                             gAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTaaa                                >  2:340847/1‑141 (MQ=255)
                                                                                                             gAAACTAAATAGGAACATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTaaa                                <  2:546521/141‑1 (MQ=255)
                                                                                                             aacaatAAATATGTAAATAATAAAGTGGAAAAAGTAAAAGTATTGCAAGATTTTAAAGAAGATTTACAAGTAGATGAAAGTTACGTAAGCTTAAAAAAAAAAAAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTaaa                                <  2:616223/136‑1 (MQ=255)
                                                                                                              aaaCTAAATATGACCATACTAAAGTGGAAATAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAg                               <  1:394474/141‑1 (MQ=255)
                                                                                                              aaaCTAAATATGACCATACTAAAGTGGAAATAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAGGTATTCTTGATAAAGAAGGTACTAAAGt                              >  1:678280/1‑141 (MQ=255)
                                                                                                                aCTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTAGTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTa                             <  1:642760/141‑1 (MQ=255)
                                                                                                                aCTAAATATGAACAGACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTa                             <  2:572592/141‑1 (MQ=255)
                                                                                                                 cTAAATATGACCATACTAAAGTGGAAATAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTaa                            >  1:518945/1‑141 (MQ=255)
                                                                                                                 cTAAAGATGACCATACTAAAGTGGAAATAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTaa                            <  1:354527/141‑1 (MQ=255)
                                                                                                                 atAAATATGAACATACGAAAGTGGATATAGTAAAAGGATTGAGAGATCGTAAAGAAGATTTACATGTAGATGAAATTTTCGGAAGCTTAAAAAAAAAAAAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTaa                            <  2:275085/140‑1 (MQ=255)
                                                                                                                  tAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGAATTCTTGATAAAGAAGGTACTAAAGTAAt                           >  2:142455/1‑141 (MQ=255)
                                                                                                                  ataaaaagcaCCATAATAAAGTGGAAATAGTAAAACTATTGAAAGGTGCTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTaa                            <  2:491318/132‑1 (MQ=255)
                                                                                                                   aaaTATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAAtt                          >  1:378558/1‑141 (MQ=255)
                                                                                                                    aaTATGACCATACTAAAATGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAAATTTACATGTAGATGAAATTTACGGAAGCCTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTa                         <  2:702927/141‑1 (MQ=255)
                                                                                                                     atatGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAATCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTaa                        <  2:597908/141‑1 (MQ=255)
                                                                                                                     atatGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTaa                        <  1:300149/141‑1 (MQ=255)
                                                                                                                       atGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAACGTAATTAAAg                      >  2:424365/1‑141 (MQ=255)
                                                                                                                       aatcaCCATCCTAAAGTGGAAAAAGTAAAAGTATTCAAAGATGTTAAAGAAGATTTACATGTAGATGAAACTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTaaa                       <  1:159420/137‑1 (MQ=255)
                                                                                                                               tACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGaa               >  2:740710/1‑140 (MQ=255)
                                                                                                                                aCTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGAca              >  1:126228/1‑140 (MQ=255)
                                                                                                                                 ctcaaGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGACTa             <  1:335348/138‑1 (MQ=255)
                                                                                                                                      ggggAAATAATAAAAGCAATGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTAAAAAAAAAAAAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGACTAAATTc        <  2:202759/139‑1 (MQ=255)
                                                                                                                                      gTGGCAAAAGTAAAAGTATTGAAAGATGTTACAGAAGATTTACATGTAGATGAAAATTACGGAAGCTTATATCATACCGAAAAAGGTCAAGGTATTCTTGATAAAGAAGGTACTCAAATAATTAAAGGTAAGACTAAATTc        >  2:308964/1‑141 (MQ=255)
                                                                                                                                          aaaaaGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGCAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAGAGAAGGTACTAAAGTAATTAAAGGTACGACTAAATTCGCaa    >  2:159481/1‑141 (MQ=255)
                                                                                                                                          aaaaaGTAAAAGTATTGAAAGATGGTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGACTAAATTCGCaa    <  1:225289/141‑1 (MQ=255)
                                                                                                                                            aaagtaaaagtaTTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGACTAAATTCGCAAAt  <  2:648286/141‑1 (MQ=255)
                                                                                                                                            aaaataaaagtaTTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGACTAAATTCGCAAAt  <  2:154054/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                            
GAAAAATGATGAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGACTAAATTCGCAAAT  >  CP000730/462078‑462357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: