Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 499,857 C→T 16.5% A854V (GCG→GTG)  gltB1 → glutamate synthase (NADPH), large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730499,8570CT16.5% 196.7 / 22.5 94A854V (GCG→GTG) gltB1glutamate synthase (NADPH), large subunit
Reads supporting (aligned to +/- strand):  ref base C (35/42);  new base T (6/9);  total (42/52)
Fisher's exact test for biased strand distribution p-value = 7.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

TTTAAAGCATACTCTGAAGCGGTGAACAAAAATAGAACAGATCACATTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTACAAGTTGATGGAAGCAACAA  >  CP000730/499718‑499995
                                                                                                                                           |                                                                                                                                          
tttAAAGCATACTCTGAAGCGGTGAACAAAAATAGAACAGATCACATTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGCCg                                                                                                                                           >  2:241536/1‑141 (MQ=255)
 ttAAAGCATACTCTGAAGCGGTGAACAAAAATAGAACAGATCACATTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGTGa                                                                                                                                          >  1:647535/1‑141 (MQ=255)
   aaaGCATACTCTGAAGCGGTGAACAAAAATAGAACAGATCACATTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGTGATg                                                                                                                                        <  2:107894/141‑1 (MQ=255)
   aaaGCATACTCTGAAGCGGTGAACAAAAATAGAACAGATCACATTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTCAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGTGATg                                                                                                                                        <  2:421315/141‑1 (MQ=255)
    aaGCATACTCTGAAGCGGTGAACAAAAATAGAACAGATCACATTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATgg                                                                                                                                       >  2:411254/1‑140 (MQ=255)
    aaGCATACTCTGAAGCGGTGAACAAAAATAGAACAGATCACATTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGATAAGTAACATTGTCAAACGCTTTAATACAGGGGAGATGa                                                                                                                                       >  1:534479/1‑141 (MQ=255)
      gCATACTCGGAAGCGGTGAACAAAAATAGAACAGATCACATTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGt                                                                                                                                     <  2:174801/141‑1 (MQ=255)
          tctctGAAGCGGTGAACAAAAATAGAACAGATCACATTACTGATTTGCTTGAATTTAAAGCATGGACACCGATTGACAGCGACCTAGTCCAACCGGTAAGTGACAGTGTCAAACGCTTTAATACAGGGGCGATGAGTGATg                                                                                                                                 <  2:223675/140‑1 (MQ=255)
           ctctGAAGCGGTGAACAAAAATAGAACAGATCACATTAGGCATTTACTTGAATTTAAAGCATGTCCACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGAGCGATGTGTTATgg                                                                                                                                >  2:101600/1‑141 (MQ=255)
              tGAAGCGGTTAACAAAAATAGAACAGATCACATTAGACATTTACTTGAATTTAAAGCATTTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATc                                                                                                                             <  2:653359/141‑1 (MQ=255)
              tGAAGCGGTGAACAAAAATAGAACAGATCACATTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTTATATAGGGGTGATGAGGTAtttaac                                                                                                                             <  2:406096/141‑6 (MQ=255)
              tGAAGCGGTGAAAAAAAATAGAACAGATCACATTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATc                                                                                                                             <  1:543365/141‑1 (MQ=255)
               gAAGCGGTGAACAGAAAGAGAACAGATCACATTAGACAGTTACTTGAATTTAAAGCATGTACACCTATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCg                                                                                                                            <  2:4270/141‑1 (MQ=255)
               gAAGCGGTGAACAAAAATAGAACAGATCACATTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGGCAGCAAACAACCTGTAAGTGACATTGTCAAACGCTTTAATACAGGGGTGATGAGTTATGGATCg                                                                                                                            >  1:774567/1‑141 (MQ=255)
               gAAGCGGTGAACAAAAATAGAACAGATCACATTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGCCCACGAGGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGTGATGAGTTATGGATCg                                                                                                                            >  1:774632/1‑141 (MQ=255)
                    ggctaaCAAAAATAGAACAGATCACATTAGACAATTGCTTGAAATTAAAGCATGTACACCGCTTGACATCAACCAAGTTGAACCGGGAAGGGCCCTTGGCACACGCGTTAATACATGGGCGAGGCGTTATGGATCGATTTc                                                                                                                       >  2:369984/5‑141 (MQ=255)
                      tGAACAAAAATAGAACAGATCACATTAGACATTGACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAg                                                                                                                     <  2:588979/141‑1 (MQ=255)
                      ggAACAAAACAAGACCAGATCCGGTTCGACATTAACTTGAATTTAAAGGCGGTACACCGATTGACAGCGACCAAGTTGAACGGGGAAGGGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGAGCGATTTCAg                                                                                                                     <  2:440686/140‑1 (MQ=255)
                                 aggaCAGATCACATTTGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACAt                                                                                                          <  2:177345/138‑1 (MQ=255)
                                 aGAACAGATCACATTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACAt                                                                                                          <  1:257715/141‑1 (MQ=255)
                                   aaCAGATCACATTAGACATCTACTTGAATTTAAAGCATGTACACCGATTGACATGGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGCGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGa                                                                                                        >  1:512673/1‑141 (MQ=255)
                                    aaaGATCACCTTCGAAATTAACCTGAAAGTAAAGAATGTAGACCGATTGACAACGACAAAGTTGAACCGAAATCTGACATTTTCAAACGCGTAACTACAGGGGCGATGAGTTATGGATCGATTTCATCGGAAGGACATGaa                                                                                                       >  2:375873/3‑141 (MQ=255)
                                       gaacacaTTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACg                                                                                                    <  1:715813/138‑1 (MQ=255)
                                          cacaTTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTa                                                                                                 >  2:628510/1‑141 (MQ=255)
                                            caTTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGc                                                                                               >  1:665673/1‑141 (MQ=255)
                                             aTTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCACACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACGTGAAACGTTAGca                                                                                              >  2:570838/1‑141 (MQ=255)
                                               tAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGcaca                                                                                            <  1:84294/141‑1 (MQ=255)
                                                aGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACaa                                                                                           <  1:647931/141‑1 (MQ=255)
                                                    atctACTTGAATTTAAAGCATGTACACCGATGGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTCCAGCGGAAGCACATGAAACGTTAGCACAAGCCa                                                                                       <  1:253145/138‑1 (MQ=255)
                                                    aTTTACTTGAATTTAAAGCCTGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCa                                                                                       <  1:334128/141‑1 (MQ=255)
                                                         cTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAAc                                                                                  >  1:349657/1‑141 (MQ=255)
                                                         cTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATAAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAAc                                                                                  <  1:525671/141‑1 (MQ=255)
                                                          ttGAATTTAAAGCATGTACACCGCTTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTGATGGATCGATTTCAGCGGAAGCACATGAGACGCTAGCACAAGCCATGAAcc                                                                                 >  2:98812/1‑141 (MQ=255)
                                                          ttGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGATATGGATCGATTTCAGCGGAAGCACATGAAAAGTTATCACAAGCCATGAAca                                                                                 >  2:652928/1‑140 (MQ=255)
                                                          ttGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGCTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAAcc                                                                                 >  2:506280/1‑141 (MQ=255)
                                                           tGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCa                                                                                <  2:721244/141‑1 (MQ=255)
                                                           tGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCa                                                                                <  2:429453/141‑1 (MQ=255)
                                                            gAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCaa                                                                               <  2:304049/141‑1 (MQ=255)
                                                              aTTGAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAAtt                                                                             <  2:251801/141‑1 (MQ=255)
                                                                ttAAAGCATGTACACCGATTGACATCTACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTGGCACAAGCCATGAACCAATTAg                                                                           >  2:661541/1‑141 (MQ=255)
                                                                ttAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCAAATGAAACGTTAGCACAAGCCATGAACCAATAAg                                                                           >  2:73835/1‑141 (MQ=255)
                                                                 tAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAgg                                                                          <  1:285032/141‑1 (MQ=255)
                                                                  aaggCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGt                                                                         <  1:271406/138‑1 (MQ=255)
                                                                   aaGGATGTACACCGATTGACATCGACCAAGTTGAACCGGGAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTg                                                                        <  2:335652/141‑1 (MQ=255)
                                                                   aaGCATGTACACCGATTGCCATCGACCAAGTTGAACCGGTAGGTGACATTGTCAAACGCTTTAATACAGGGGCGGTGAGTAGTGGATCGATTTCAGCGAAGGCACATGAAAGGTTGACACACGCCACGAACaaagaagcgg                                                                        >  2:260365/1‑134 (MQ=255)
                                                                   aaGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTg                                                                        >  1:205401/1‑141 (MQ=255)
                                                                    aGCATGTACACCTATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTgg                                                                       <  1:559076/141‑1 (MQ=255)
                                                                    aGCATGCACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTgg                                                                       <  2:543317/141‑1 (MQ=255)
                                                                     gCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGa                                                                      <  1:566079/141‑1 (MQ=255)
                                                                        tGTACACCGATTGACATCGACCCAGTTGTGCCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACGAGCCATGAACCAATTAGGTGGAAag                                                                   >  1:580349/1‑141 (MQ=255)
                                                                        tGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGGGGAAag                                                                   >  2:399714/1‑141 (MQ=255)
                                                                        tGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGCGGAAag                                                                   >  1:757887/1‑141 (MQ=255)
                                                                           acacCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGt                                                                >  2:303035/1‑141 (MQ=255)
                                                                              ccGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGTGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAAt                                                             <  1:67431/141‑1 (MQ=255)
                                                                              ccGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGTGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAAt                                                             <  1:67692/141‑1 (MQ=255)
                                                                               cGATTGACATCGACCCAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGTGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATGAGGTGGAAAGGGTAATa                                                            >  2:226693/1‑141 (MQ=255)
                                                                               cGATTGACATCGACCAAGTTGAACCGGTAAGTGACATGGTCAAACGCTTTAATACAGGGGTGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGAAATa                                                            >  1:319176/1‑141 (MQ=255)
                                                                                gATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAg                                                           >  2:590986/1‑141 (MQ=255)
                                                                                  ttGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGGGGAAAGAGTAATAgtg                                                         >  1:108650/1‑141 (MQ=255)
                                                                                    gACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGTGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAgtggt                                                       >  1:135549/1‑141 (MQ=255)
                                                                                     aCATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAgtggtg                                                      >  2:419255/1‑141 (MQ=255)
                                                                                       aTCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGTAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATTgtggtgca                                                    >  2:362090/1‑139 (MQ=255)
                                                                                          gACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGGGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAACGAGTAATAATGGTGAAggg                                                 >  2:510453/1‑140 (MQ=255)
                                                                                             cAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGc                                              <  2:146308/141‑1 (MQ=255)
                                                                                                 ttGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAg                                          <  1:510313/141‑1 (MQ=255)
                                                                                                    aaCCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGTGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATg                                       <  1:209683/141‑1 (MQ=255)
                                                                                                    aaCCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATg                                       >  1:323207/1‑141 (MQ=255)
                                                                                                    aaCCGGTAAGGGACATTGGCAAACGCTTTAATACAGGGGTGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTGAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATg                                       <  2:321909/141‑1 (MQ=255)
                                                                                                    aaCCGGGAAGTGACATTGGCAAACGCTTTAGTACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATg                                       <  2:491627/141‑1 (MQ=255)
                                                                                                    aaCCGGGAAGTGACATGGGTAAACGCTTTAATACGGGGGGGAGGAGTGATGGAGCGATGTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAAGTAGGTGGAAAGAGTAATAGGGGTGAAGGTGGCGAAGATg                                       <  1:550711/141‑1 (MQ=255)
                                                                                                      ccGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGCAGGTGGCGAAGATGCa                                     <  1:392292/141‑1 (MQ=255)
                                                                                                            aGTGACATTGTCAAACGCTTTAATACAGGGGCGCTTAATTACTTGTCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGt                               <  2:553045/141‑1 (MQ=255)
                                                                                                            aGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGCTCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGt                               >  2:422140/1‑141 (MQ=255)
                                                                                                            aGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGt                               <  1:464769/141‑1 (MQ=255)
                                                                                                            aGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGt                               <  2:103606/141‑1 (MQ=255)
                                                                                                            aGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATGAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGt                               <  1:499331/141‑1 (MQ=255)
                                                                                                            aGTGACATTGTAAAACGATACAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGt                               <  1:5230/141‑1 (MQ=255)
                                                                                                            aGTGACATCGGCAAACGCTTTAAGTCAGTGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGt                               <  2:175659/141‑1 (MQ=255)
                                                                                                                  aTTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGAAAAACGTCATGaa                         >  2:354737/1‑141 (MQ=255)
                                                                                                                     gTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTa                      >  2:353791/1‑141 (MQ=255)
                                                                                                                       cAAACGCTTTAATACAGGGGCGATGAGTTATGGAGCGATTTCAGCGGAAGAGCACCATGTGCTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTACa                    <  1:774017/141‑1 (MQ=255)
                                                                                                                       cAAACGCTTTAACACAGGGGCGAGGATTTATGGATTGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTACa                    <  1:340836/141‑1 (MQ=255)
                                                                                                                        aaaCGCTTTAATACAGGGGTGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTACaa                   <  2:774632/141‑1 (MQ=255)
                                                                                                                        aaaCGCTGTAAAACAGGGGTGAGGAGTTATGGATCGATTTCAGCGGAAGCACAGGAAACGTTAGCACAAGCCATGAACCCATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTACaa                   <  2:774567/141‑1 (MQ=255)
                                                                                                                         aaCGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTACAAg                  <  1:241536/141‑1 (MQ=255)
                                                                                                                          aCGCTTTAATACAGGGGCGGTGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTACAAGt                 <  2:455607/141‑1 (MQ=255)
                                                                                                                            gCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCCGCGGAAGCACATGAAACGTTGGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAAGAGTGGGGAAGGTGGCGAGTATGCAAAACGTTATGAAGTAGAAGTTg               >  2:472990/1‑141 (MQ=255)
                                                                                                                                  aTACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTACAAGTTGATGGaa         >  1:709143/1‑141 (MQ=255)
                                                                                                                                  aTACAGGGGCGATGAATCATGGATCGATTTCAGCGTAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGGAGGTGGCGAAGATGCAAAACGTTATGAAGTACAAGTTCATGGaa         <  2:464890/141‑1 (MQ=255)
                                                                                                                                   tACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTACAAGTTGATGGAAg        >  2:644083/1‑141 (MQ=255)
                                                                                                                                   tACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTACAAGTGGATGGAAg        >  2:703136/1‑141 (MQ=255)
                                                                                                                                     cAGGGGCTATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTACAAGTTGATGGAAGca      <  2:219005/141‑1 (MQ=255)
                                                                                                                                     cAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTACAAGTTGATGGAAGca      <  2:652159/141‑1 (MQ=255)
                                                                                                                                         ggCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTACAAGTTGATGGAAGcaacaa  >  1:659637/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TTTAAAGCATACTCTGAAGCGGTGAACAAAAATAGAACAGATCACATTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTTGAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATTTCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAATAGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTACAAGTTGATGGAAGCAACAA  >  CP000730/499718‑499995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: