Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 567,389 A→T 60.8% Q95L (CAA→CTA)  ctsR → CtsR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730567,3890AT60.8% 34.6 / 65.4 56Q95L (CAA→CTA) ctsRCtsR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (11/11);  new base T (15/19);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 7.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

ACGATTCACTAATGAACATGGTTATGAAATCGAAAGTAAACGTGGTGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCAATGGATATGGTTTCTAGAGATATTATTAGA  >  CP000730/567249‑567522
                                                                                                                                            |                                                                                                                                     
acGATTCACTAATGAACATGGTTATGAAATCGAAAGTAACCGTGGTTGCGGTGGTTACTTTCGAATCTCTAAAATTGAAAATAAGGATGCACCAGGTTATATTAATCATTTGCTTCAGCTGATTGGCCCTTCTATTTCTca                                                                                                                                       <  2:279811/141‑1 (MQ=255)
acGATTCACTAATGAACATGGTTATGAAATCGAAAGTAAACGTTGTGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCACCAGGTCATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTca                                                                                                                                       <  1:347630/141‑1 (MQ=255)
  gATTCACTAATGAACATGGTTATGAAATCGAAAGTAAACGTGGTGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTctac                                                                                                                                     >  2:733274/1‑141 (MQ=255)
   aTTCACTAATGGACATGGTTATGAAATCGAAAGTAAACGAGGGGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTctaca                                                                                                                                    >  1:359663/1‑141 (MQ=255)
   aTTCACTAATGAACATGGTTATGAAATCGAAAGTAAACGTGGTGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTcaaca                                                                                                                                    <  2:28333/141‑1 (MQ=255)
    ttCACTAATGAACATGGTTATGAAATCGAAAGTAAACGTGGTGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTcaacaa                                                                                                                                   <  1:35852/141‑1 (MQ=255)
       aCTAATGAACATGGTTATGAAATCGAAAGTAAACGTGGTGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTctacaacaa                                                                                                                                <  1:35472/141‑1 (MQ=255)
              aaCATGGTTATGAAATCGAAAGTAAACGTGGTGGTGGTGGTTACCTCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTAGAATAATCATTTGCTTCAGCTGCTTGGACCTTCTATTTCTCTACAACAAGCttatt                                                                                                                         >  2:433826/1‑141 (MQ=255)
               aCATGGTTATGAAATCGAAAGTAAACGTGGTGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTTGACCTTCTATTTCTCAACAACAAGCttatta                                                                                                                        >  2:407381/1‑141 (MQ=255)
                cATGGTTATGAAATCGAAAGTAAACGTGGTGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTtattat                                                                                                                       <  2:677381/141‑1 (MQ=255)
                cATGGTTATGAAATCGAAAGTAAACGTGGTGGTGGTGGGTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTCGTTCAGCTGATTGGACCTTCTATTTCTCTACAACAGGCTtattat                                                                                                                       >  1:301107/1‑141 (MQ=255)
                   ggTTATGAAATCGAAAGTAAACGTGGTGGTGGTGGTTAATGACGAATCGCTTTTAAGCACTTGTGAGAGGGAACACGTTATATTAATCATTTTCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTtattatatt                                                                                                                    <  2:555537/141‑1 (MQ=255)
                       aggAAATCGAAAGTAAACGTGGTGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTg                                                                                                                <  2:371999/139‑1 (MQ=255)
                       aTGAAACCGAAAGTAAACGTGGTGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTg                                                                                                                <  2:309693/141‑1 (MQ=255)
                        tGAAATCGAAAGTAAACGTGGTGGTGGTTGTTACATCCGAATCACTCCTATTGAGAAGAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGa                                                                                                               <  1:219811/141‑1 (MQ=255)
                        tGAAATCGAAAGTAAACGTGGTGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGa                                                                                                               >  2:535949/1‑141 (MQ=255)
                        tGAAATCGAAAGTAAACGTGGTGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGa                                                                                                               >  2:353259/1‑141 (MQ=255)
                        tGAAATCGAAAGTAAACGTGGTGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGa                                                                                                               <  1:518999/141‑1 (MQ=255)
                         gAAATCGAAAGTAAACGTGGTGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGAt                                                                                                              >  1:265287/1‑141 (MQ=255)
                                aaaGTAAACGTGGTGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCAACAACAAGCTTATTATATTAATGATGGGCttt                                                                                                       >  2:406653/1‑141 (MQ=255)
                                aaaGTAAACGTGGGGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCAACAACAAGCTTATTATATTATTGATGGGCttt                                                                                                       <  1:35121/141‑1 (MQ=255)
                                               gtggtggtTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTtaa                                                                                        <  1:73761/141‑1 (MQ=255)
                                                      ttACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTTTTGATGGGCTTTTAGATAAAATGTTAATAAATGa                                                                                 >  1:533336/1‑141 (MQ=255)
                                                       tACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGaa                                                                                <  1:450863/141‑1 (MQ=255)
                                                           tCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTAATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGATCGTg                                                                            <  1:19750/141‑1 (MQ=255)
                                                           tCCGAATCACTAAAATAGAGAATAAAGATGCAACAGGTGATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTg                                                                            >  1:441430/1‑141 (MQ=255)
                                                              gAATCACTAAAAATGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATCGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAg                                                                         >  1:557768/1‑141 (MQ=255)
                                                                aTCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCt                                                                       <  2:446210/141‑1 (MQ=255)
                                                                        aaTTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGAt                                                               <  1:15553/141‑1 (MQ=255)
                                                                           tGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCa                                                            <  1:316473/141‑1 (MQ=255)
                                                                            gcaaaTAAAGATGCAACAGGGTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCaa                                                           <  1:212215/139‑1 (MQ=255)
                                                                              aaaTAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAga                                                         >  2:543934/1‑140 (MQ=255)
                                                                              aaaTAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGc                                                         >  1:193945/1‑141 (MQ=255)
                                                                                aTAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAg                                                       >  2:523914/1‑141 (MQ=255)
                                                                                     gATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTAtt                                                  >  1:117281/1‑141 (MQ=255)
                                                                                         cAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATa                                              <  2:13855/141‑1 (MQ=255)
                                                                                           aaaGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATaga                                            <  2:153068/139‑1 (MQ=255)
                                                                                           aCAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTATCAGCACCAAGCGTGTTATATTATTGATGGGCTTTTAGATAAAATATTAAGAAATAGACGTGAAGGTAGGATGATTTAAGCAGTTATTGagagg                                            >  1:457711/1‑140 (MQ=255)
                                                                                             aGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCAACAACAAGCTTACTATATTATTGATGGCCTTCTACATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAgaga                                          <  1:663563/141‑1 (MQ=255)
                                                                                                  atatTAATCATTTGCGTCAGCTTATTGGTCCTTCTATTTCTCTACAATAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATAAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGc                                     <  1:357177/141‑1 (MQ=255)
                                                                                                   tatTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCt                                    >  2:12970/1‑141 (MQ=255)
                                                                                                   tatTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCt                                    >  1:461689/1‑141 (MQ=255)
                                                                                                   tatTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCAACAACAAGCTTACTATATTATTGCTGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGATAAAATACTTCACGCAGTTATTGATAGAAAAACGCt                                    >  2:161357/1‑141 (MQ=255)
                                                                                                    atTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTa                                   <  2:624915/141‑1 (MQ=255)
                                                                                                       aaTCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATCAATTAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCa                                <  1:523914/141‑1 (MQ=255)
                                                                                                         tCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATGGATAGAGAAACGCTATCaag                              >  1:120818/1‑140 (MQ=255)
                                                                                                            tttGCTTCAGCTGATTGGACCTTCTATTTCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCAATGGa                           <  2:776836/141‑1 (MQ=255)
                                                                                                                cTTCAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCAATGGATATg                       <  2:763190/141‑1 (MQ=255)
                                                                                                                   cAGTTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCAATGGATATGGtt                    <  2:265287/141‑1 (MQ=255)
                                                                                                                   cAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCAATGGATATGGtt                    <  1:784023/141‑1 (MQ=255)
                                                                                                                   cAGCTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCAATGGATATGGtt                    <  1:783630/141‑1 (MQ=255)
                                                                                                                      cTGATTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCAATGGATATGGTTTCt                 >  1:51438/1‑141 (MQ=255)
                                                                                                                      cTGATTGGACCTTCTATTGCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCAATGGATATGGTTTCt                 <  2:259261/141‑1 (MQ=255)
                                                                                                                         aTTGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATGGATAGAGAAACGCGATCAATGGATATGGTTGCTagg              >  2:428280/1‑140 (MQ=255)
                                                                                                                          ttGGACCTTCTATTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAAAGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGATATCAATGGATATGGTTTCTagag             >  1:766805/1‑141 (MQ=255)
                                                                                                                          ttGGACCTTCTATTTCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCAATGGATATGGTTTCTagag             <  1:188278/141‑1 (MQ=255)
                                                                                                                            ggACCTTCTATTTCTCAACAACAAGCTTATTATATTTTTGATGGGCTTTTAGATAAAAGGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCAATGGATATGGTTTCTAGAGat           <  1:20101/141‑1 (MQ=255)
                                                                                                                            ggACCTTCTATTTCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCAATGGATATGGTTTCTAGAGat           <  1:4094/141‑1 (MQ=255)
                                                                                                                            ggACCTTCTATTCCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCAATGCATCTGTGTTCTAGAGat           <  2:597217/141‑1 (MQ=255)
                                                                                                                                   cTATTTCTCTACAACAAGCTTATTATATTATTGATGGACTTTTAGATGAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCGATGGATATGGTTTCTAGAGATAttatta    >  2:593635/1‑141 (MQ=255)
                                                                                                                                     aTTTCTCTACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCAATGGATATGGTTTCTAGTGATATTATTagg  >  2:662048/1‑140 (MQ=255)
                                                                                                                                     aTTTCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCAATGGATATGGTTTCTAGAGATATTATTagc  >  1:270338/1‑140 (MQ=255)
                                                                                                                                     aTTTCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCAATGGATATGGTTTCTAGAGATATTATTaga  >  2:179709/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                     
ACGATTCACTAATGAACATGGTTATGAAATCGAAAGTAAACGTGGTGGTGGTGGTTACATCCGAATCACTAAAATTGAAAATAAAGATGCAACAGGTTATATTAATCATTTGCTTCAGCTGATTGGACCTTCTATTTCTCAACAACAAGCTTATTATATTATTGATGGGCTTTTAGATAAAATGTTAATAAATGAACGTGAAGCTAAAATGATTCAAGCAGTTATTGATAGAGAAACGCTATCAATGGATATGGTTTCTAGAGATATTATTAGA  >  CP000730/567249‑567522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: