Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,083,479 T→C 6.5% G33G (GGT→GGC purD → phosphoribosylamine‑‑glycine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,083,4790TC 6.5% 184.1 / 3.5 67G33G (GGT→GGCpurDphosphoribosylamine‑‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base T (32/30);  new base C (3/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

ACTCGACATTTTAAACACTAATTTTAAAGGGGCGTAACATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTGGGTAGTTATAGGTCCAGAACAGCCGCTAATTGATGGAT  >  CP000730/1083339‑1083615
                                                                                                                                            |                                                                                                                                        
cgtCGACATTTTAAACACTAGTTGTAAAGGGGCGTAACATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGt                                                                                                                                          <  2:145177/139‑1 (MQ=255)
aCTCGACATTTTAAACACTAATTTTAAAGGGGCGTAACATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGt                                                                                                                                          >  1:304755/1‑141 (MQ=255)
aCTCGACATTTTAAACACTAATTTTAAAGGGGCGTAACATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGt                                                                                                                                          <  2:531412/141‑1 (MQ=255)
   cGACATTTTAAACACTAATTTTAATGGGGCGTAACATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCATTCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAAt                                                                                                                                       <  2:493501/141‑1 (MQ=255)
         tttAAACACTAATTTTAAAGGGGCGTAACATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCa                                                                                                                                 <  2:503384/141‑1 (MQ=255)
                   aaTTTTAAAGGGGCGTAACATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATAGAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGCAATGAGGCAATGACACCta                                                                                                                       >  2:86444/1‑141 (MQ=255)
                   aaTTTTAAAGGGGCGTAACATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTACATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGCAATGAGGCAATGACACCta                                                                                                                       >  2:86296/1‑141 (MQ=255)
                     ttttAAAGGGGCGAAACATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCtata                                                                                                                     <  2:331735/141‑1 (MQ=255)
                         aaaGGGGCGTAACATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTg                                                                                                                 <  1:85689/141‑1 (MQ=255)
                             gggCGTAACATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGt                                                                                                             >  2:641494/1‑141 (MQ=255)
                             gggCGTAACAGAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGt                                                                                                             >  2:533938/1‑141 (MQ=255)
                                   aaCATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAGACATGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTacacac                                                                                                       >  1:375079/1‑141 (MQ=255)
                                   aaCATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTacacac                                                                                                       >  2:83564/1‑141 (MQ=255)
                                   aaCATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTacacac                                                                                                       <  1:462760/141‑1 (MQ=255)
                                    aCATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACt                                                                                                      >  2:561983/1‑141 (MQ=255)
                                    aCAGAAGTGAATGTATCCGTAATTGGTGCTGGTGGACGAGAACACGCCCTTGCATATCAACTTAATCAATCGAATCCAGTTAAACACGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACt                                                                                                      <  2:33342/141‑1 (MQ=255)
                                     cATAAATGAATCTACTAGTATTTGGTGCTGTTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTg                                                                                                     <  2:511088/141‑1 (MQ=255)
                                          atgaatgTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTCCACCCTGAGAtt                                                                                                >  1:33303/1‑141 (MQ=255)
                                          atgaatgTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACAAACTGAAAtt                                                                                                >  1:96060/1‑141 (MQ=255)
                                              atgGATTAGTAATTGGTGCTGGTGGACGAGAATATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAg                                                                                            <  1:630593/141‑1 (MQ=255)
                                                 tATTAGTAATTGGTGCGTGTGGACGAGAACGTGCCCTTGCAGATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAAt                                                                                         <  2:585401/141‑1 (MQ=255)
                                                     aGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGa                                                                                     <  1:175415/141‑1 (MQ=255)
                                                     aGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGa                                                                                     <  1:175412/141‑1 (MQ=255)
                                                     aGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAATTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGa                                                                                     >  2:675040/1‑141 (MQ=255)
                                                          ttGGTGCTGGTGGACGATAACATGCACCTGCCTATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGatcatc                                                                                <  1:289866/141‑1 (MQ=255)
                                                          ttGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTGATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTGCACAATCTGataatc                                                                                >  1:652263/1‑141 (MQ=255)
                                                          ttGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGatcatc                                                                                >  1:309446/1‑141 (MQ=255)
                                                            ggTGCTGGTGGACGAGGACGTGCACTTGCATATAAACTTAAGCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCaa                                                                              >  2:382929/1‑141 (MQ=255)
                                                            ggTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGCATCTCGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCaa                                                                              >  1:23380/1‑141 (MQ=255)
                                                            ggTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGGCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACAAACCGAAATTTCCGAATCGGATCATCaa                                                                              >  1:519561/1‑141 (MQ=255)
                                                               gCTGGTGGACGAGAACATGCACTTGAATACAAAATTACACAATCGAATCTAGTTAAACAAGTGATTGTCATACCAGGCAATGAGGCAAAAACACATATAGCTGAAGTACACACGGGAATTTCAGAACATGATCATCAAGCg                                                                           >  2:245797/1‑141 (MQ=255)
                                                                  ggtggACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATa                                                                        >  2:773132/1‑141 (MQ=255)
                                                                    tggACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCAGTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACt                                                                      <  2:110546/141‑1 (MQ=255)
                                                                    gggACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACt                                                                      <  1:611091/140‑1 (MQ=255)
                                                                     ggACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGCATCTGATCAACAAGCGATACTa                                                                     >  2:307300/1‑141 (MQ=255)
                                                                     ggACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTa                                                                     >  1:647447/1‑141 (MQ=255)
                                                                         gagaACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAATTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTCATCATCCAGCGATACTAGAtt                                                                 <  2:539961/141‑1 (MQ=255)
                                                                                       gCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCaa                                                   <  2:712638/141‑1 (MQ=255)
                                                                                         ttaggaaCTTGATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTAGCTAAACGGCaaaa                                                 <  2:538288/136‑1 (MQ=255)
                                                                                               aCTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGa                                           <  1:761652/141‑1 (MQ=255)
                                                                                                cTTAATCAATGGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTgct                                          >  1:571528/1‑139 (MQ=255)
                                                                                                cTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGCAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGAt                                          <  1:86296/141‑1 (MQ=255)
                                                                                                cTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGCAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGAt                                          <  1:86444/141‑1 (MQ=255)
                                                                                                 ttatccaagcGAATCTAGGTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGTATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGAtt                                         <  2:306939/136‑1 (MQ=255)
                                                                                                 ttAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGAtt                                         <  2:106856/141‑1 (MQ=255)
                                                                                                 ttAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGAtt                                         <  2:652551/141‑1 (MQ=255)
                                                                                                 ttAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGAtt                                         <  2:48290/141‑1 (MQ=255)
                                                                                                   aatcaatcGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGCTCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTgg                                       >  1:735255/1‑141 (MQ=255)
                                                                                                   aatcaatcGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTgg                                       >  2:665110/1‑141 (MQ=255)
                                                                                                   aatcaatcGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTgg                                       >  1:338651/1‑141 (MQ=255)
                                                                                                     tcaatcGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGCACCCCATGTCAGCAAGCCAGCTTAGCGTCAAACGTCTCCAGGTTTTGCTAAACGGCAACATGTTGATTGGGt                                     <  2:555968/141‑1 (MQ=255)
                                                                                                      caatcGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTGGGTa                                    <  2:727178/141‑1 (MQ=255)
                                                                                                        atcGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTgggaggt                                  >  1:211340/1‑137 (MQ=255)
                                                                                                        atcGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCAAATCGTAAAGCAAAACTGGATATTGCTAAACGACAAATTGTTGAATGGGGAGt                                  >  1:105105/1‑141 (MQ=255)
                                                                                                             aTCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATATCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTGGGTAGTTATAg                             >  1:742350/1‑141 (MQ=255)
                                                                                                             aTCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTGGGTAGTTATAg                             >  2:613297/1‑141 (MQ=255)
                                                                                                             aTCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAAAGTTGATTGGTTAGTTagag                             >  2:139748/1‑141 (MQ=255)
                                                                                                             aTCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGACAAAATGTTGATTGGGTAGTTATAg                             >  2:338286/1‑141 (MQ=255)
                                                                                                                  gTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTGGGTAGTTATAGGTCCa                        >  2:221119/1‑141 (MQ=255)
                                                                                                                   ttgaaCAAGTGGTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTGGGTAGTTATAGGTCCAg                       <  2:701890/138‑1 (MQ=255)
                                                                                                                   ttAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTGGGTAGTTATAGGTCCAg                       >  1:620538/1‑141 (MQ=255)
                                                                                                                   gtgaaCAAGTGGTTGTCATTCCAGCTAATGAGTAAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGGTACTAGATTTTGCTAAACGGCAAAATGTTGATTGGGTAGTTATAGGTCCAg                       <  2:188114/138‑1 (MQ=255)
                                                                                                                    tAAACAAGTGTTTGTCATTCCAGTTAATGAGGCAATGACACCTATCGCGGAAGTACACCCTGAAATTTCAGAATCTGAGCTTCAAGCGTGACTAGCTTTTGCTAAACGGCAAAATGTTGATTGGGTAGTTATAGGTCCAGa                      <  2:349790/141‑1 (MQ=255)
                                                                                                                               ttGTCATTCCAGGTAATGAGGCAATGACACATATAGCTGAAGTACCCACTGAAATTTCGGAATCTAATCATCACACGATACTAGATTGCGCTAAACGGCAGAATTGTGAGTGTTTAGTTATAGGTCCAGAACAGCCGCTaa           >  2:577403/1‑141 (MQ=255)
                                                                                                                               tgttCATTCCAGGTAAGGCGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTGGGTAGTTATATGTCCAGAACAGCCGCTaa           <  2:23380/138‑1 (MQ=255)
                                                                                                                                   cATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACAGACGGAGATGTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTGGGTAGTTATAGGTCCAGAACAGCCGCTAATTGa       >  1:152657/1‑141 (MQ=255)
                                                                                                                                   cATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGCACAAGGTGGAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAGACGGCAAAATGTTGATTGGGTAGTTATAGGTCCAGAACAGCCGCCAATTGa       >  1:541701/1‑141 (MQ=255)
                                                                                                                                    aTTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTGGGTAGTTATAGGCCCAGAACAGCCGCTAATTGAt      >  2:448686/1‑141 (MQ=255)
                                                                                                                                        cAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTGGGTAGTTATAGGTCCAGAACAGCCGCTAATTGATGGAt  <  1:294132/141‑1 (MQ=255)
                                                                                                                                        cAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTGGGTAGTTATAGGTCCAGAACAGCCGCTAATTGATGGAt  <  1:354194/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                        
ACTCGACATTTTAAACACTAATTTTAAAGGGGCGTAACATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACAAGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAGCGATACTAGATTTTGCTAAACGGCAAAATGTTGATTGGGTAGTTATAGGTCCAGAACAGCCGCTAATTGATGGAT  >  CP000730/1083339‑1083615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: