Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,693,509 A→G 5.3% L10S (TTA→TCA)  hrcA ← heat shock transcriptional repressor HrcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,693,5090AG 5.3% 219.8 / 3.2 75L10S (TTA→TCA) hrcAheat shock transcriptional repressor HrcA
Reads supporting (aligned to +/- strand):  ref base A (35/36);  new base G (2/2);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.62e-01

TCTCGATATAGTTTAAATCTTCAAGCTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAATTTTTTTAATCAT  >  CP000730/1693369‑1693644
                                                                                                                                            |                                                                                                                                       
tctcGATATAGTTTAAATCTTCAAGCTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATACTCTTCAACAATTGCGTTTa                                                                                                                                         >  1:552204/1‑141 (MQ=255)
   cGATATAGTTTAAATCTTCAAGCTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTATAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAata                                                                                                                                      <  1:525058/141‑1 (MQ=255)
   cGATATAGTTTAAATCTTCAAGCTGTTTCATATCATTTCTAATTGTGGCAGGACTAACATTCACGTTATGTCGCTCAAGTAGTGTTTTAGATCGAACGGGTTGTCCAAAATCAAGATAATCCTCAAGAATTGCGTTTAata                                                                                                                                      >  2:215777/1‑141 (MQ=255)
     atatAGTTTAAATCTTCAAGCTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGGTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAAtata                                                                                                                                    >  1:649653/1‑141 (MQ=255)
      tataGTTTAAATCTTCAAGCTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATAc                                                                                                                                   <  2:526745/141‑1 (MQ=255)
      tataGTTTAAATCTTCAAGCTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATAc                                                                                                                                   <  2:410846/141‑1 (MQ=255)
      tataGTTTAAATCTTCAAGCTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTGATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATAc                                                                                                                                   <  1:531415/141‑1 (MQ=255)
      tataGTTTAAATCTTCAAGCTGTTTCATCTCATTTCCAAATGGATCAGTACTTAGATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATAc                                                                                                                                   <  2:277773/141‑1 (MQ=255)
      tataGTTTAAATCTGCAAGCTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATAc                                                                                                                                   <  1:9099/141‑1 (MQ=255)
      tataCTTTAAATCTTCGAGCTGTTTCATCTCATTTCTAATTGTAGCAGGACGAACATTGAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATAc                                                                                                                                   <  2:769891/141‑1 (MQ=255)
      aataGTTTAAATTTTCAAGCTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACCATTGCGTTTAATATAc                                                                                                                                   <  1:392721/140‑1 (MQ=255)
                 tCTTCAAGCTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCtt                                                                                                                        >  2:2294/1‑140 (MQ=255)
                 tCTTCAAGCTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTa                                                                                                                        <  1:304621/141‑1 (MQ=255)
                 tCTTCAAGCTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTa                                                                                                                        >  2:228283/1‑141 (MQ=255)
                 tCTTCAAGCGGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTa                                                                                                                        <  2:458033/141‑1 (MQ=255)
                    tCAAGCTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATCTACTCAATTGCCTATCt                                                                                                                     >  1:684662/1‑141 (MQ=255)
                     cAAGCTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTg                                                                                                                    >  1:84644/1‑141 (MQ=255)
                      aaGCTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGt                                                                                                                   <  2:340345/141‑1 (MQ=255)
                         cTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAAt                                                                                                                <  2:248125/141‑1 (MQ=255)
                          tGTTTCATTTCATTTCTAATTGGAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCTAAAATCAACATAATCCTCACCAATTGCGTTTAATATACTCAATTGCCTATCTGTAATc                                                                                                               <  2:342437/141‑1 (MQ=255)
                          agTTTTAGCTCATATCTAAGTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGGTTAATATACTCAATTGCCTATCTGTAATc                                                                                                               <  1:746778/140‑1 (MQ=255)
                             ttCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTGATATACTCAATTGCCTATCTGTAATCATg                                                                                                            >  1:562872/1‑141 (MQ=255)
                             ttCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTGATATACTCAATTGCCTATCTGTAATCATg                                                                                                            >  1:687738/1‑141 (MQ=255)
                              tCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTTAATTGCCTATCTGTAATCATGt                                                                                                           <  2:576429/141‑1 (MQ=255)
                               cATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGtt                                                                                                          >  1:635702/1‑141 (MQ=255)
                                  ctcATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTc                                                                                                       <  1:265309/141‑1 (MQ=255)
                                     aTTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCAcc                                                                                                    >  2:22815/1‑141 (MQ=255)
                                     aTTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCAcc                                                                                                    >  1:279753/1‑141 (MQ=255)
                                        tCTAATTGTAGCAGGACTATCATTCTAGTTATGTCGCTCATTTAGTGTTTTATAACCAACGGGTTGTCCAAAATCAACATAATCCTCATCAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCa                                                                                                 <  2:27236/141‑1 (MQ=255)
                                         ttAAGTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCAt                                                                                                <  1:94709/140‑1 (MQ=255)
                                         cTAATTGGAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCAt                                                                                                <  1:138207/141‑1 (MQ=255)
                                          tAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCAtt                                                                                               <  1:519086/141‑1 (MQ=255)
                                          tAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCAtt                                                                                               >  1:345540/1‑141 (MQ=255)
                                            aTTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAg                                                                                             >  1:700158/1‑141 (MQ=255)
                                               gTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGcac                                                                                          >  2:759445/1‑141 (MQ=255)
                                                        cTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTAtc                                                                                 >  2:297916/1‑141 (MQ=255)
                                                        cTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTGTAACATTGTCAGTTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTAtc                                                                                 >  1:181931/1‑141 (MQ=255)
                                                        cTAACATTCAAGTTATGTCGATCACTATGTTGGTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTAtc                                                                                 >  2:559081/1‑141 (MQ=255)
                                                          aaCGTTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTAtctc                                                                               <  2:555139/141‑1 (MQ=255)
                                                           aCATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATGCTCAACAATTGCGTGTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTaaacca                                                                              >  2:59735/1‑136 (MQ=255)
                                                           aCATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCa                                                                              <  2:532229/141‑1 (MQ=255)
                                                           aCATTCAAGTTATGTCGCTAAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCATCATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTATTCATGTTTTCACCTCATTAGCACTCACTTATCTCa                                                                              <  2:337023/141‑1 (MQ=255)
                                                            cATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCaa                                                                             <  1:534986/141‑1 (MQ=255)
                                                            aaTTAAATTTATGTCGCGCAATTAGTGTTTTAAAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCaa                                                                             <  1:635635/140‑1 (MQ=255)
                                                              ttCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGt                                                                           <  2:84644/141‑1 (MQ=255)
                                                                      aTGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATCTTAACTAGTTCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATta                                                                   >  1:775489/1‑141 (MQ=255)
                                                                       tGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATtat                                                                  >  1:640726/1‑141 (MQ=255)
                                                                       tGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATtat                                                                  >  1:94194/1‑141 (MQ=255)
                                                                       tGTCGCTCAATTAGTGTTTTAGAACCAACGAGTTGTCCAAAATCAACATAATCCTCAACAGTTGCGTTTAATATACTCAATTGCATATATGTAATCATGTTTTATCCTCATTAGAACTCCCTTATCTCAGATGCAATTtat                                                                  >  2:410654/1‑141 (MQ=255)
                                                                       tGTCGCTCAATTAGGGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATtat                                                                  >  1:514898/1‑141 (MQ=255)
                                                                               aaTTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATc                                                          <  2:638941/141‑1 (MQ=255)
                                                                                 ttAGTGTTTTAGAACCAGCGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGATTCCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCaa                                                        <  2:278807/141‑1 (MQ=255)
                                                                                 tgtgagtgttAGAACCCACGGGTTCTCCAAAACCAACATAATCCTCATCAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCTCTCACTTATCTCAAGTGCTAATTATAATTTATCaa                                                        <  1:364947/133‑1 (MQ=255)
                                                                                     tgtTTTAGAACCAACGGGTGATTCGGTATGAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTAGCTCAAGTGCTAATTATAATTTATCAGATTg                                                    <  2:550464/141‑1 (MQ=255)
                                                                                             aaCCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGCTCAcagt                                            >  1:746624/1‑141 (MQ=255)
                                                                                                cAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAacgtaag                                         >  2:788838/1‑141 (MQ=255)
                                                                                                  aCGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTc                                       >  2:308496/1‑141 (MQ=255)
                                                                                                   cGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAACGTAAGTCa                                      >  2:80685/1‑141 (MQ=255)
                                                                                                     ggTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAATTAAGTAAAt                                    >  1:364481/1‑141 (MQ=255)
                                                                                                      gTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATg                                   >  1:89019/1‑141 (MQ=255)
                                                                                                                aTCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTc                         >  1:470370/1‑141 (MQ=255)
                                                                                                                     cATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAAt                    >  1:769336/1‑141 (MQ=255)
                                                                                                                      aTAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAAtt                   >  2:631688/1‑141 (MQ=255)
                                                                                                                      aTAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAAtt                   <  2:76432/141‑1 (MQ=255)
                                                                                                                           ccTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAAt              >  1:559036/1‑141 (MQ=255)
                                                                                                                            cctaactagtGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGGCAATTTTAAAGACTCGAAATTTCAAtt             <  2:169065/132‑1 (MQ=255)
                                                                                                                              caacaaTTGCCTTTAATATACTCAATTGCCTATCGGCAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAGGACTCGAAATTTCAAtttt           <  2:536570/141‑1 (MQ=255)
                                                                                                                               aacaaTTGCGTTTGATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAAttttt          <  2:687738/141‑1 (MQ=255)
                                                                                                                               aacaaTTGCGTTTGATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAAGGTCAAttttt          <  2:562872/141‑1 (MQ=255)
                                                                                                                                  aaTTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAAtttttttt       >  2:75826/1‑140 (MQ=255)
                                                                                                                                  aaTTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAATTTTTTTa       <  2:190078/141‑1 (MQ=255)
                                                                                                                                  aaTTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAATTTTTTTa       <  1:221586/141‑1 (MQ=255)
                                                                                                                                    ttGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAATTTTTTTAAt     <  1:130836/141‑1 (MQ=255)
                                                                                                                                    ttGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAATTGTTCTaaa     >  2:277102/1‑140 (MQ=255)
                                                                                                                                       cGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAATTTTTTTAATCAt  >  2:652647/1‑141 (MQ=255)
                                                                                                                                       cGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAATTTTTTTAATCAt  >  2:114650/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                       
TCTCGATATAGTTTAAATCTTCAAGCTGTTTCATCTCATTTCTAATTGTAGCAGGACTAACATTCAAGTTATGTCGCTCAATTAGTGTTTTAGAACCAACGGGTTGTCCAAAATCAACATAATCCTCAACAATTGCGTTTAATATACTCAATTGCCTATCTGTAATCATGTTTTCACCTCATTAGCACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAATTTTTTTAATCAT  >  CP000730/1693369‑1693644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: