Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,812 G→A 64.6% intergenic (‑895/‑273) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8120GA64.6% 121.6 / 125.6 139intergenic (‑895/‑273)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (31/18);  new base A (38/51);  total (69/70)
Fisher's exact test for biased strand distribution p-value = 3.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 149 total aligned reads displayed.

TCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC  >  CP000730/1929672‑1929950
                                                                                                                                            |                                                                                                                                              
tCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtag                                                                                                                                                <  1:716088/141‑1 (MQ=255)
 cGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagt                                                                                                                                               >  2:426748/1‑141 (MQ=255)
         ttCTTAAATTACCAATATCTATGCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTACACTCATCTTCCCTACCAGTTGCATGTTATTGTGCTTGTATATTTGCCGATGAAATATTAGTAGAGTCGc                                                                                                                                       <  2:166902/141‑1 (MQ=18)
             tAATTTAACAATATCTATTCGCATAGAATTTGTCCAATTAAGTGTAGACGAGTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAt                                                                                                                                   <  1:9718/141‑1 (MQ=255)
             tAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAt                                                                                                                                   <  1:399358/141‑1 (MQ=255)
                   aaCAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCa                                                                                                                             <  2:491713/141‑1 (MQ=255)
                     cAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAgg                                                                                                                           >  1:756113/1‑141 (MQ=255)
                      aaTATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGc                                                                                                                          <  2:3515/141‑1 (MQ=255)
                              ttCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAAttt                                                                                                                  >  2:270743/1‑141 (MQ=255)
                                     aGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCa                                                                                                           >  2:403482/1‑141 (MQ=255)
                                      gAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCag                                                                                                          <  1:510033/141‑1 (MQ=255)
                                      gAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGGAGAGTCGCCTATCTCTAAGGCGCCAATTTAGACGCag                                                                                                          >  2:671500/1‑141 (MQ=255)
                                               cAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCCGAGaggaggg                                                                                                 >  1:592540/1‑140 (MQ=255)
                                                aaTTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGCTACAATGGTAGAGTCGCCTATCTCCCAGGCGTCAATTTAGACGCAGAGAGGAGgtg                                                                                                >  1:22294/1‑141 (MQ=255)
                                                        gaaGACGATGCAATCTAACAACTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTTGAGTAGACTATCACCCCGTTTCCAATTTTGACGCAGAGAGGAGGTGTATACGGt                                                                                    >  2:553076/3‑141 (MQ=255)
                                                          aGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGa                                                                                  >  2:534310/1‑141 (MQ=255)
                                                                     tCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTc                                                                       >  1:234925/1‑141 (MQ=255)
                                                                     tCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGGGTATAAGGTGATGCTTATTTTc                                                                       >  1:89949/1‑141 (MQ=255)
                                                                              tAGAATCATATAACCTATCAGAAGCATCTGATTGGGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTACTTTCGTTCAcatc                                                              <  2:510327/141‑1 (MQ=255)
                                                                                  aTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAg                                                          >  1:120961/1‑141 (MQ=255)
                                                                                       ataACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCa                                                     <  2:629134/141‑1 (MQ=255)
                                                                                            ttATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGtcat                                                >  1:396041/1‑141 (MQ=255)
                                                                                               tCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAg                                             >  2:702850/1‑141 (MQ=255)
                                                                                                 aGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTg                                           >  2:21626/1‑141 (MQ=255)
                                                                                                  atcggaagtagatatgCTTGTTTATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                         <  1:248677/127‑1 (MQ=255)
                                                                                                  atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                         <  1:243169/127‑1 (MQ=255)
                                                                                                  atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                         >  2:216025/15‑141 (MQ=255)
                                                                                                     ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                      <  1:30215/130‑1 (MQ=255)
                                                                                                     ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                      <  2:780147/130‑1 (MQ=255)
                                                                                                     ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAAGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCagt                                      >  2:295305/12‑141 (MQ=255)
                                                                                                      gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                     >  2:446819/11‑141 (MQ=255)
                                                                                                      gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                     <  2:202120/131‑1 (MQ=255)
                                                                                                      gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCCCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTCCGTCCACATCATAGCACCAGTCATCAGTGGCtgtg                                     <  1:781285/131‑1 (MQ=255)
                                                                                                      cAAGTGACTGTGCTTGTATTGTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                      <  2:712038/141‑1 (MQ=255)
                                                                                                       aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                    <  1:648254/132‑1 (MQ=255)
                                                                                                       aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                    <  1:194823/132‑1 (MQ=255)
                                                                                                       aagtagacatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCCCCTCACAGCCCCAGTCATCAGTGGCTGTGc                                    <  2:494119/132‑1 (MQ=255)
                                                                                                        agttgatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                   <  2:340707/133‑2 (MQ=255)
                                                                                                        agtaggtatgCTTGTAAATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGTTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtacg                                   <  2:134359/133‑4 (MQ=255)
                                                                                                        agtagatatgCTTGTATATTCACATATGGTATAATAATAGAGTCGAATCTCACGCAGGCGTCAATT‑‑GACGAAGAGATTAGTTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                   <  1:771550/133‑2 (MQ=255)
                                                                                                        agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                   <  2:293117/133‑2 (MQ=255)
                                                                                                        agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                   <  2:447388/133‑2 (MQ=255)
                                                                                                        agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                   <  1:175539/133‑2 (MQ=255)
                                                                                                        aggagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                   <  1:220785/133‑2 (MQ=255)
                                                                                                        agcggatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                   <  2:223559/133‑2 (MQ=255)
                                                                                                         gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                  <  1:17166/134‑3 (MQ=255)
                                                                                                         gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAAGCATCAGTGGCTGTGctt                                  >  1:648556/8‑139 (MQ=255)
                                                                                                         gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                  >  1:665767/8‑139 (MQ=255)
                                                                                                         gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                  <  1:540274/134‑3 (MQ=255)
                                                                                                           agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATAATTGGCAGTGctgtt                                >  1:562840/6‑137 (MQ=255)
                                                                                                            gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGgtgttctgttt                               >  1:297441/5‑134 (MQ=255)
                                                                                                             ttatgCTTGTATATTCACAGTTGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                              <  2:119996/138‑7 (MQ=255)
                                                                                                             atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                              <  2:285063/138‑7 (MQ=255)
                                                                                                             atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGc                              >  2:612588/4‑141 (MQ=255)
                                                                                                             agatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                              <  2:612720/138‑7 (MQ=255)
                                                                                                              tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTTTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgcg                             <  2:73757/139‑8 (MQ=255)
                                                                                                              tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCCCCAGTCATCAGTGGCTGTGCTGTTGCg                             >  2:707829/3‑141 (MQ=255)
                                                                                                              tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                             >  1:564133/3‑141 (MQ=255)
                                                                                                              ggtgGTTGTAGGTTTGCCGATGAGATAATTGTGGAGGCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATCTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                              <  2:25965/140‑1 (MQ=255)
                                                                                                              gatgcgggTATATTCCCAGATGGTATAAAAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTGCACATCATAGCACCAGCCATCAGTGGCTGTGctgttgcg                             <  2:72792/134‑8 (MQ=255)
                                                                                                               ttgCTTGTATATTCACAGATGGTATAATAATAGAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATGGGACCATTCATCAGTGGCTGTGCTGTTGCGt                            <  2:398364/140‑1 (MQ=255)
                                                                                                               gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCCGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTATTGCg                             >  2:76694/1‑141 (MQ=255)
                                                                                                               atgCTTGTATATTCAAAGATGGTATAATAATAGAGACGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGGTGTGCTGTTGCGt                            <  2:479971/140‑1 (MQ=255)
                                                                                                                   ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                        <  1:361405/141‑5 (MQ=255)
                                                                                                                   ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                        <  1:132123/141‑5 (MQ=255)
                                                                                                                   ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                        <  2:631932/141‑5 (MQ=255)
                                                                                                                    tGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAGCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                        <  1:663620/141‑1 (MQ=255)
                                                                                                                    tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                       <  1:228803/141‑6 (MQ=255)
                                                                                                                    tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                       <  1:729979/141‑6 (MQ=255)
                                                                                                                     gTATATTTGCCGATGATATACTAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGCCGCAGAGAGGAGGTGTATAAGGTGATGCTTATTGTCGTTCACATCATAGCACCGGTCATCCGTGGCTGTGCCATTTCGattttt                       >  2:242328/1‑141 (MQ=255)
                                                                                                                      tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                     <  1:333845/141‑5 (MQ=255)
                                                                                                                      tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                     <  2:58166/141‑8 (MQ=255)
                                                                                                                      tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                     <  1:474462/141‑8 (MQ=255)
                                                                                                                       atattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatact                    >  1:69526/10‑136 (MQ=255)
                                                                                                                       atatTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttttttt                     >  1:613147/1‑140 (MQ=255)
                                                                                                                          ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                 >  2:589971/7‑141 (MQ=255)
                                                                                                                           tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                >  2:780498/6‑141 (MQ=255)
                                                                                                                            cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCGATT‑‑GACGAAGAGAGGAGGAGCATTACTTTGCTAATCATTTCCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTTCGTACTATACTTATTg               >  2:229152/5‑141 (MQ=255)
                                                                                                                            cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg               >  1:213155/5‑141 (MQ=255)
                                                                                                                            cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg               >  1:431598/5‑141 (MQ=255)
                                                                                                                             acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg              <  1:446819/138‑1 (MQ=255)
                                                                                                                             acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATACTACTTATTgg              >  2:373499/4‑141 (MQ=255)
                                                                                                                              cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GAAGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc             <  2:646429/139‑1 (MQ=255)
                                                                                                                                gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTAACATCATAGCACCAGTCATCATTGGCTGTGCGGTTGCGTATTATACTTATTGGCtt           >  1:374746/1‑140 (MQ=255)
                                                                                                                                gatagtataATAAGAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt           <  2:40769/136‑2 (MQ=255)
                                                                                                                                 atggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta          >  2:764145/5‑139 (MQ=255)
                                                                                                                                 actatatAATATTGGCGTGACCTTTCTTTAAGGGCTCAACTTACACGCAGTGGGGCGGTGTTGCAGGTGATGCTTACTTTGGTTCAGCTCATGGCTCCGGTTATTAGTGGCTGTGCCATTGCGTTTTTTTCTTCTTGGCTa           <  2:17241/138‑1 (MQ=255)
                                                                                                                                  tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag         >  1:588513/4‑138 (MQ=255)
                                                                                                                                  tgctataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGATAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag         <  1:146154/138‑4 (MQ=255)
                                                                                                                                   ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCAAAACATGGCACCAGTCATCAGTGGCTGTGCTGTTGCGTAATATACTTATTGGCttcgt        >  2:198286/3‑137 (MQ=255)
                                                                                                                                   ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt        >  2:603051/3‑141 (MQ=255)
                                                                                                                                   ggtagaataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt        <  2:115824/136‑1 (MQ=255)
                                                                                                                                     tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa      >  1:47314/1‑140 (MQ=255)
                                                                                                                                     tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa      >  2:18834/1‑140 (MQ=255)
                                                                                                                                      ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTGTTGGCTTAGTaaa     >  2:599572/1‑139 (MQ=255)
                                                                                                                                      ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa     <  1:309192/141‑3 (MQ=255)
                                                                                                                                      ataataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAGTGGCTAGGTAg      >  2:185483/1‑141 (MQ=255)
                                                                                                                                       taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc    >  1:613689/1‑141 (MQ=255)
                                                                                                                                        aataGTTGGCTCGCCTGTCTCCTAGTCGTCAATTTAGACGCAGAGAGTAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGAc    <  2:743024/141‑1 (MQ=255)
                                                                                                                                          tagtagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCCGTGGCTGTGCCATTTCGTTTTTTTCTTATTGACTAGTTAGACGc  >  2:416952/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                              
TCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC  >  CP000730/1929672‑1929950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: