BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,970	T→C	60.2%	E194E (GAA→GAG)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,970	0	T	C	60.2%	548.0 / inf	844	E194E (GAA→GAG)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (165/169); new base C (262/242); total (429/415)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 846 total aligned reads displayed.

AACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAA  >  CP000730/35848‑36108

        atatATCACGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGc                                                                                                                  <  1:400701/138‑1 (MQ=0)

                   cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAACTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                                                       >  1:215644/1‑141 (MQ=2)

                     gtgtTCGTTTAACTTGCTAGCATGATGCTAATTGCGTGGCATGGCGAAAATAAGTAGATTTGAAGAAACCTGCTGTTCTTTTTATATAGAGCGTAAAGACACTTAATACCTTTTAAAGTATTCTTTGCTGTATTTATACtt                                                                                                     >  2:716532/1‑141 (MQ=11)

                       gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg                                                                                                   >  2:729820/1‑141 (MQ=11)

                       gtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                                                                                   >  2:292952/1‑141 (MQ=17)

                        ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                                                  <  2:265078/141‑1 (MQ=32)

                        ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                                                  >  2:57287/1‑141 (MQ=17)

                         tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTCTGAAGAGACCCGCGGTGCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                                                 <  2:475428/141‑1 (MQ=11)

                         gcgtttAACTTGCCAGCATGATGCTAGTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGAt                                                                                                 <  2:599006/137‑1 (MQ=11)

                          cactgaaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCAGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTCAAGTATTCTTTGCTGTATTGATACTTTGATa                                                                                                >  2:249484/6‑141 (MQ=255)

                            tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                                                                              <  1:634917/140‑3 (MQ=255)

                            cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                              <  1:645285/141‑1 (MQ=17)

                                aCTTGCTAGCAGGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGCTATCTTGt                                                                                          <  2:694712/141‑1 (MQ=11)

                                 cTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTGAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGAGATCTTGtc                                                                                         >  1:693908/1‑141 (MQ=11)

                                 cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                         <  2:307825/141‑1 (MQ=17)

                                    gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                      <  1:683601/141‑1 (MQ=17)

                                    gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                      <  1:41849/141‑1 (MQ=17)

                                     cTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttc                                                                                     <  1:28482/141‑1 (MQ=11)

                                      tAGCATGATGGTAATTCCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTCAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                    <  2:220799/141‑1 (MQ=255)

                                      tAGCATGATGCTAATTTCGTTGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                    <  1:101260/141‑1 (MQ=17)

                                      tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTCTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                    <  2:49602/141‑1 (MQ=17)

                                          atgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACt                                                                                >  2:787238/1‑141 (MQ=11)

                                          atgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACt                                                                                >  1:77989/1‑141 (MQ=18)

                                           tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                                                                               >  2:622483/1‑141 (MQ=25)

                                              tgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTaa                                                                            >  2:390696/1‑141 (MQ=21)

                                               gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                                                                           >  1:365950/1‑141 (MQ=34)

                                                cTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTCCTTTAata                                                                          >  2:666680/1‑141 (MQ=11)

                                                 tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                                                                         >  2:111606/1‑141 (MQ=35)

                                                 tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                                                                         <  1:675118/141‑1 (MQ=25)

                                                  aaTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGCGAACTACGGTTCTATTTATAGAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTACTACTTTAATATCTTGTCTTTCTTACCTTAATATg                                                                        >  2:279935/1‑141 (MQ=11)

                                                  aaTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTCAATACATTCAATAACGTTTAAAGTATTATTTGCCGTATTGATACTTTGATATCTTGTTTTTCTTACTTTAatatc                                                                        >  2:632813/1‑140 (MQ=11)

                                                  aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                        <  1:177066/141‑1 (MQ=25)

                                                   aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGa                                                                       >  1:317080/1‑141 (MQ=255)

                                                   aTTTCGTGGCATGGCGAAAATCCGTAGATCGGGAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTATTTGGTGTATTGATAATTTGAGACTTAGTGTTTCGTACGTTAATATGa                                                                       >  1:244934/1‑141 (MQ=11)

                                                     ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGCCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTGGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg                                                                     <  2:418382/141‑1 (MQ=255)

                                                     ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg                                                                     <  2:505935/141‑1 (MQ=37)

                                                     ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg                                                                     <  2:546795/141‑1 (MQ=37)

                                                      tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                                                    <  1:460910/141‑1 (MQ=38)

                                                       cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                   >  1:303307/1‑141 (MQ=38)

                                                        gTGGCATGGCGAAAATCCGTAGATCTGACGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                  <  1:548398/141‑1 (MQ=255)

                                                           gCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATc                                                               >  1:533059/1‑141 (MQ=255)

                                                            cATGGGGGAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATAAATTCAATACCTTTTAAAGTATTCTTTGCCGTCTTGATACTTTGATATCTTGTCTTTCTTTCTTTAATGTGACgagtgtct                                                              >  2:180500/1‑134 (MQ=11)

                                                             aTGGGGAAAATCCGTAGCTCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAAGACATTCAATACCTTTTATAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                                             <  1:739185/141‑1 (MQ=14)

                                                             aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                                             <  1:564939/141‑1 (MQ=255)

                                                              tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATTTTg                                                            >  2:68115/1‑141 (MQ=37)

                                                               tgggAAAATCCGTAGATCTGAAGAGACCTACTGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                           <  1:511228/138‑1 (MQ=11)

                                                               ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                           <  1:487632/141‑1 (MQ=39)

                                                               ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                           >  1:712158/1‑141 (MQ=38)

                                                                ggtaaaaTCCGTAGATCTGAAGAGTCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATCGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                                                          <  2:639112/138‑1 (MQ=11)

                                                                  gAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATCTGACGGTGATATTGCTCa                                                        >  2:161748/1‑141 (MQ=255)

                                                                   aaaaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCaa                                                       <  1:237237/141‑1 (MQ=255)

                                                                    aaaTCCGTAGATCTGAAGAGACCTAGGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGTCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCCCAAt                                                      >  1:261120/1‑141 (MQ=11)

                                                                    aaaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATATTTTGATATCTTTTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                      >  2:652435/1‑141 (MQ=11)

                                                                         cGTAGATCTGAAGAGTCCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTGAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                 >  1:165816/1‑141 (MQ=255)

                                                                         cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCACAATGAGGt                                                 >  2:14526/1‑141 (MQ=255)

                                                                          gTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAGTGAgtgg                                                >  1:335997/1‑138 (MQ=14)

                                                                             gATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                             <  1:626950/141‑1 (MQ=255)

                                                                              aTCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAACGAGGTTATTc                                            >  2:441276/1‑141 (MQ=255)

                                                                               tCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATAACGGTGATCTTGCTCAATGAGGTTATTCa                                           >  2:319546/1‑141 (MQ=11)

                                                                                 tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                         <  1:706983/141‑1 (MQ=255)

                                                                                 tGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                         <  2:100338/141‑1 (MQ=255)

                                                                                  gAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATACTGTGCTGTATGGATACTTTGCTACGTTGTCGTTCTTACTTGAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGat                                        >  2:197981/1‑141 (MQ=11)

                                                                                   aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata                                       <  1:349048/141‑1 (MQ=255)

                                                                                   aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAACACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata                                       >  2:431493/1‑141 (MQ=255)

                                                                                   aaGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTTTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGCCGGTTATCTTGTTCAATAAGGTTATTCAGatt                                       >  1:758870/1‑140 (MQ=11)

                                                                                     ggtgccTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTCCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                     <  2:3814/137‑1 (MQ=255)

                                                                                       gaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTc                                   >  1:39092/1‑141 (MQ=38)

                                                                                        aCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg                                  >  1:602022/1‑141 (MQ=255)

                                                                                            gCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                              >  2:235281/1‑141 (MQ=39)

                                                                                            gCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCGGATGTTTCGATGt                              >  1:80435/1‑141 (MQ=255)

                                                                                            gCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                              >  2:128179/1‑141 (MQ=38)

                                                                                             cGGTTCTTTGTATATAGAGCGTAAATACATTCAATATCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATATCGTGTCTTTCTTACTGTGATTTGACACTGATCTTGCGCTAGGAGGCTATTCAGATATTTCGATGTa                             <  2:362781/141‑1 (MQ=11)

                                                                                               tttCTTTTTATATAGAGCGGAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACa                           <  1:57697/140‑1 (MQ=255)

                                                                                               gTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTCTTCAGATATTTCGATGTACa                           <  2:591664/141‑1 (MQ=255)

                                                                                                   tttttATATGGAGCGAAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCGGATATTTCGATGTACAATGa                       >  1:584174/1‑141 (MQ=14)

                                                                                                   tttttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATCCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCCATGAGGTTATTCAGATATTTCGATGTACAATgg                       >  2:85608/1‑140 (MQ=255)

                                                                                                    ttttATATAGAGCTTAAATACACTCAATACCTTTTAAAGTTTTCTTTGTTGTATTGATACTTTGATACCTTGTCTTGCTTACTTTAATACGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATggc                      <  2:767225/141‑3 (MQ=14)

                                                                                                     tttATATCGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACa                     <  1:192794/141‑1 (MQ=255)

                                                                                                     tttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATggca                     <  1:126627/141‑4 (MQ=255)

                                                                                                      ttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGCTGTACAATGACAg                    >  1:664920/1‑141 (MQ=255)

                                                                                                      ttACATAGAGCGTAAATACACTCAATACCTTTTAAAGTATAATTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAg                    >  2:517777/1‑141 (MQ=14)

                                                                                                       tatataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                   <  2:405373/141‑1 (MQ=255)

                                                                                                        ttataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTc                  <  1:609882/140‑1 (MQ=255)

                                                                                                        atataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTc                  >  1:723557/1‑141 (MQ=255)

                                                                                                          ataGAGCGTAAATACAGCCAATACCTTTTAAAGTATTCTTTGCTGTATGGATACGTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCCTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAg                <  1:111590/141‑1 (MQ=14)

                                                                                                           taGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAgg               <  1:447224/141‑1 (MQ=255)

                                                                                                            agagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgt              >  1:275725/1‑141 (MQ=255)

                                                                                                             gagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtt             >  1:136183/1‑141 (MQ=255)

                                                                                                              tgtgTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttt            <  2:352768/138‑1 (MQ=255)

                                                                                                              agCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttt            <  1:147813/141‑1 (MQ=255)

                                                                                                                cGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaa          >  2:456859/1‑141 (MQ=255)

                                                                                                                  tAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagt        <  1:502051/141‑1 (MQ=255)

                                                                                                                   aaaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGCCAGTCAGgtttagatt       >  2:468922/1‑137 (MQ=255)

                                                                                                                   aaaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTGAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt       >  2:537947/1‑141 (MQ=255)

                                                                                                                    aaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACGGGTAGggttaagttt      >  2:340021/1‑141 (MQ=14)

                                                                                                                    aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGTCGTACGCGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt      >  1:376210/1‑141 (MQ=14)

                                                                                                                     aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta     <  1:428505/141‑1 (MQ=255)

                                                                                                                     aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta     >  1:470433/1‑141 (MQ=255)

                                                                                                                      tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttgagtttaa    >  2:216137/1‑141 (MQ=255)

                                                                                                                      tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCCGATATTTCGATATTGCATCGCATTCAGgtttaagtttaa    >  2:752427/1‑141 (MQ=11)

AACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAA  >  CP000730/35848‑36108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`