New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 = 230615748 (1.230)4 (0.120) 4/244 4.9 9.1% coding (78/108 nt) USA300HOU_2165 hypothetical protein
?CP000730 = 2306167 38 (1.110)coding (68/108 nt) USA300HOU_2165 hypothetical protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCTCAACGTTTGGTAAAACGAATCGCGTTATTATCGATACTGAAGACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACAGTTGTCCTTTTTCACATATATTTATAACAAAAGATGTGCCATCAAAGAAATAATTGG  >  CP000730/2306028‑2306301
                                                                                                                                            |                                                                                                                                      
gCTCAACGTTTGGTAAAACGAATCGCGTTATTATCGATACTGAAGACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTc                                                                                                                                       >  1:299395/1‑141 (MQ=255)
  tCAACGTTTGGTAAAACGAATCGCGTTATTATCGATACTGAAGACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCat                                                                                                                                     >  1:44487/1‑141 (MQ=255)
   cAACGTTTGGTAAAACGAATCGCGTTATTATCGATACTGAAGACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCatg                                                                                                                                    <  2:357796/141‑1 (MQ=255)
    aaCGTTTGGTAAAACGAATCGCGTTATTATCGATACTGAAGACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCatga                                                                                                                                   >  1:353681/1‑141 (MQ=255)
     aCGTTTGGTAAAACGAATCGCGTTATTATCGATACTGAAGACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCatgaa                                                                                                                                  >  1:63891/1‑141 (MQ=255)
        tttGGTAAAACGAATCGCGTTATTATCGATACTGAAGACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCatgaatga                                                                                                                               <  1:264803/141‑1 (MQ=255)
          tGGTAAAACGAATCGCGTTATTATCGATACTGAAGACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGAtt                                                                                                                             <  2:61673/141‑1 (MQ=255)
            gTAAAACGATTCGCGTTATTATCGATACTGAAGACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGAtttt                                                                                                                           <  2:358583/141‑1 (MQ=255)
            gTAAAACGAATCGCGTTATTATCGATACTGAAGACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAAATCATTCATGGTGAGGGCTTCATGAATGAttt                                                                                                                            >  1:438305/1‑141 (MQ=255)
                       cgcgTTATTATAGATACTGAAGACCACGAATACATTATTTATACTCAAAATGTGCAAAAAGTTTACAATGAATTAACTAAATAAAGTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGAtt                                                                                                                <  2:318946/141‑1 (MQ=255)
                            tattatCGATACTGAAGACCACGAATACATTATTTATACGCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAaca                                                                                                           >  2:67243/1‑141 (MQ=255)
                                  cGATACTGAAGACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAAAGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGc                                                                                                     >  2:263851/1‑141 (MQ=255)
                                   gATACTGAAGACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCa                                                                                                    <  2:164425/141‑1 (MQ=255)
                                   gATACTGAAGACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCa                                                                                                    <  2:260024/141‑1 (MQ=255)
                                    aTACTGAAGACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCAt                                                                                                   <  1:432640/141‑1 (MQ=255)
                                         gAAGACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACa                                                                                              <  2:249884/141‑1 (MQ=255)
                                            gACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAAt                                                                                           >  1:46887/1‑141 (MQ=255)
                                                aCGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAACTAAATCGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAGCACCCAGCATAAACAAATACaa                                                                                       >  2:91782/1‑141 (MQ=255)
                                                aCGAATACATTATTTATACTCAAAATGATCAAAAAGTTGACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACaa                                                                                       >  2:91783/1‑141 (MQ=255)
                                                   aaTACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAg                                                                                    >  1:47152/1‑141 (MQ=255)
                                                      aCATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGAc                                                                                 <  1:47764/141‑1 (MQ=255)
                                                        attattTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACaa                                                                               >  1:16615/1‑141 (MQ=255)
                                                                taCTCAAAATGATCAAAAAGTTTACAAAGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTccc                                                                       <  1:263851/141‑1 (MQ=255)
                                                                 aCTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCa                                                                      <  1:127550/141‑1 (MQ=255)
                                                                   tCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCAAAAAAAAAAAAAAAAGGACAACTGTTCCCATa                                                                    <  2:170046/141‑1 (MQ=255)
                                                                    cAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATaa                                                                   >  1:124692/1‑141 (MQ=255)
                                                                      aaaTGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAAtt                                                                 >  1:305249/1‑141 (MQ=255)
                                                                      aaaTGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAAtt                                                                 <  1:188775/141‑1 (MQ=255)
                                                                        aTGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAAtttt                                                               <  2:145896/141‑1 (MQ=255)
                                                                           aTCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAAc                                                            >  2:437562/1‑141 (MQ=255)
                                                                            tCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACa                                                           >  1:335061/1‑141 (MQ=255)
                                                                            tCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACa                                                           >  1:318021/1‑141 (MQ=255)
                                                                                aaaGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACAGTTg                                                       <  1:194460/141‑1 (MQ=255)
                                                                                        cAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACAGTTGTCCttttt                                               <  1:23062/141‑1 (MQ=255)
                                                                                            gAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACAGTTGTCCGTTTTcaca                                           >  2:360760/1‑141 (MQ=255)
                                                                                               ttAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACAGTTGTCCTTTTTCACatat                                        <  2:63891/141‑1 (MQ=255)
                                                                                                 aaCTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACAGTTGTCCTTTTTCACatatat                                      >  2:407484/1‑141 (MQ=255)
                                                                                                  aCTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACGCCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACAGTTGTCCTTTTTCACATATAtt                                     >  1:64507/1‑141 (MQ=255)
                                                                                                           atTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACAGTTGTCCTTTTTCACATATATTTATAACaaa                            >  2:352682/1‑141 (MQ=255)
                                                                                                           atTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACAGTTGTCCTTTTTCACATATATTTATAACaaa                            <  1:400146/141‑1 (MQ=255)
                                                                                                                   aaaaaaaTCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACAGTTGTCCTTTTTCACATATATTTATAACAAAAGAtgtg                     >  2:408674/1‑141 (MQ=255)
                                                                                                                   aaaaaaaTCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAAGTGATTCAACACCCAGCATAAACAAATACAAACGGACAACTGTTCCCATAATTTTAACAGTTGTCCTTTTTCACATATATTTATAACAAAAGAtgtg                     <  2:315338/141‑1 (MQ=255)
                                                                                                                     aaaaaTCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACAGTTGTCCTTTTTCACATATATTTATAACAAAAGATGTGcc                   <  1:289479/141‑1 (MQ=255)
                                                                                                                          tcattcatGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACAGTTGTCCTTTTTCACATATATTTATAACAAAAGATGTGCCATCaa              <  2:409802/141‑1 (MQ=255)
                                                                                                                           cattcatGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACAGTTGTCCTTTTTCACATATATTTATAACAAAAGATGTGCCATCaaa             <  2:335282/141‑1 (MQ=255)
                                                                                                                                 tGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATATACAAATACAAAAGGACAACTGTTCCCATAATTTTAACAGTTGTCCTTTTTCACATATATTTATAACAAAAGATGTGCCATCAAAGAaata       >  1:173362/1‑141 (MQ=255)
                                                                                                                                      aGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACAGTTGTCCTTTTTCACATATATTTATAACAAAAGATGTGCCATCAAAGAAATAATTgg  >  2:298692/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                      
GCTCAACGTTTGGTAAAACGAATCGCGTTATTATCGATACTGAAGACCACGAATACATTATTTATACTCAAAATGATCAAAAAGTTTACAATGAATTAACTAAATAAATTGTATAAAAAAATCATTCATGGTGAGGGCTTCATGAATGATTTTTTTAATTGATTCAACACCCAGCATAAACAAATACAAAAGGACAACTGTTCCCATAATTTTAACAGTTGTCCTTTTTCACATATATTTATAACAAAAGATGTGCCATCAAAGAAATAATTGG  >  CP000730/2306028‑2306301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.