Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,000,9480CT70.4% 31.6 / 18.7 27T11I (ACA→ATA) spxApossible glutaredoxin
Reads supporting (aligned to +/- strand):  ref base C (4/4);  new base T (10/9);  total (14/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTATAATAAATAGTAACGATTAAGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGACGGTACTGATGAAAT  >  CP000730/1000808‑1001083
                                                                                                                                            |                                                                                                                                       
ttATAATAAATAGTAACGATTAAGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGcac                                                                                                                                         >  2:353647/1‑141 (MQ=255)
               aCGATTAAGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGc                                                                                                                          >  1:75242/1‑141 (MQ=255)
                cGATTAAGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCg                                                                                                                         <  1:366298/141‑1 (MQ=255)
                          ttttAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGt                                                                                                               <  2:118971/141‑1 (MQ=255)
                           tttAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGtt                                                                                                              <  2:14260/141‑1 (MQ=255)
                           tttAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGtt                                                                                                              >  1:69978/1‑141 (MQ=255)
                               gAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACaa                                                                                                          >  1:61308/1‑141 (MQ=255)
                                         ttttttCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACa                                                                                                >  1:448054/1‑141 (MQ=255)
                                              tCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCg                                                                                           <  1:118704/141‑1 (MQ=255)
                                                          aCTAAATTTTAAGCAATGAATTTTATTCATTCAAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGt                                                                               <  2:392201/141‑1 (MQ=255)
                                                           cTAAATTTTAAGCAATGAATTTTATTCATTCAAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTa                                                                              >  2:349367/1‑141 (MQ=255)
                                                            tAAATTTTAAGCAATGAATTTTATTCATTCAAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTaa                                                                             <  2:208504/141‑1 (MQ=255)
                                                                           gAATTTTATTCATTCAAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACa                                                              <  1:58469/141‑1 (MQ=255)
                                                                                  attcattcAAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACa                                                       >  2:357770/1‑141 (MQ=255)
                                                                                     cattcaAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAAtt                                                    <  2:269308/141‑1 (MQ=255)
                                                                                                 agagTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAg                                        <  1:209350/141‑1 (MQ=255)
                                                                                                   agTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCa                                      >  1:393863/1‑141 (MQ=255)
                                                                                                        gaTGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAatat                                 >  2:99907/1‑141 (MQ=255)
                                                                                                         aTGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATAtt                                >  1:202385/1‑141 (MQ=255)
                                                                                                           gTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTaa                              >  2:178100/1‑141 (MQ=255)
                                                                                                               ggTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAAt                          >  2:297800/1‑141 (MQ=255)
                                                                                                                     attattTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGa                    <  1:29724/141‑1 (MQ=255)
                                                                                                                         tttACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGAc                >  2:198841/1‑141 (MQ=255)
                                                                                                                         tttACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGAc                <  2:263554/141‑1 (MQ=255)
                                                                                                                         tttACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGAc                >  1:419928/1‑141 (MQ=255)
                                                                                                                               tCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGACGGTACt          <  2:303657/141‑1 (MQ=255)
                                                                                                                                 aCCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGACGGTACtga        <  1:54991/141‑1 (MQ=255)
                                                                                                                                       ttGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGACGGTACTGATGAAAt  >  1:109760/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                       
TTATAATAAATAGTAACGATTAAGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAATAGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGACGGTACTGATGAAAT  >  CP000730/1000808‑1001083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑