Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,180 C→T 26.0% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,1800CT26.0% 299.7 / 81.5 148intergenic (+238/‑896)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (56/50);  new base T (22/16);  total (81/67)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

Only 100 of 148 total aligned reads displayed.

AATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTA  >  CP000730/76042‑76319
                                                                                                                                          |                                                                                                                                           
aaTTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCta                                                                                                                                           <  2:727009/141‑1 (MQ=255)
     aaTATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGta                                                                                                                                      <  2:450422/141‑1 (MQ=255)
     aaTATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGta                                                                                                                                      <  1:226508/141‑1 (MQ=255)
     aaTATAACGAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGta                                                                                                                                      <  1:196832/141‑1 (MQ=255)
      atatAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTCTAGtat                                                                                                                                     >  2:245162/1‑141 (MQ=255)
      atatAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGtaa                                                                                                                                     >  2:833324/1‑140 (MQ=255)
              aaTATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTaa                                                                                                                             <  1:78085/141‑1 (MQ=255)
              aaTATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTaa                                                                                                                             <  2:304386/141‑1 (MQ=255)
              aaTATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTaa                                                                                                                             <  2:304568/141‑1 (MQ=255)
                    gctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACaaaaa                                                                                                                       >  1:826564/1‑141 (MQ=255)
                     ctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTCTAGTATAATTTTAACAAAAAg                                                                                                                      >  2:240398/1‑141 (MQ=255)
                     ctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAg                                                                                                                      >  1:636424/1‑141 (MQ=255)
                     ctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAg                                                                                                                      >  2:942439/1‑141 (MQ=255)
                       gctcccgTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGa                                                                                                                    <  1:567410/135‑1 (MQ=255)
                             gTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAACAAGGAGtcttc                                                                                                              >  1:578541/1‑141 (MQ=255)
                                    ttAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGa                                                                                                       <  1:354326/141‑1 (MQ=255)
                                      aGAACAGCTAACCAAAGTGTTAGTAAGCCGAGGAATACTAACACAAAGGCAAGTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTAGAATTTTAACAAAAAGGAGTCTTCTGTATGAAc                                                                                                     <  2:56605/141‑1 (MQ=37)
                                             cTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCa                                                                                              <  1:341514/141‑1 (MQ=255)
                                             cTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCa                                                                                              <  1:666264/141‑1 (MQ=255)
                                                     aGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGCACTATTTCATATATaaa                                                                                      >  2:1109795/1‑141 (MQ=255)
                                                     aGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTATTCTGTATGAACTATTTCAGATATaaa                                                                                      >  2:247499/1‑141 (MQ=255)
                                                         ttAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAt                                                                                  >  1:51616/1‑141 (MQ=255)
                                                           aGTAATCCAGGGAATACTAAGACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTCTTGCAAAGTTGAATCTTTAGTAAAAAAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                                <  2:800079/141‑1 (MQ=25)
                                                           aGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                                >  1:974772/1‑141 (MQ=255)
                                                                 ccAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAg                                                                          >  1:767842/1‑141 (MQ=255)
                                                                    gggAATACTAACACAAAGGCAATTGATTTAATAATTATAGCAAAATATAGGTTATGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGAt                                                                       >  1:807833/1‑141 (MQ=255)
                                                                    gggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGAt                                                                       >  2:521470/1‑141 (MQ=255)
                                                                    gagAATACTAACACAAAGGTAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGAt                                                                       <  1:223/139‑1 (MQ=255)
                                                                           cTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCa                                                                >  2:603002/1‑141 (MQ=255)
                                                                                acaAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTa                                                           >  1:323413/1‑141 (MQ=255)
                                                                                acaAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTa                                                           >  1:39792/1‑141 (MQ=255)
                                                                                   aaGGCAATTAATTTAATATTTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                        <  1:66209/141‑1 (MQ=255)
                                                                                   aaGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                        <  2:850834/141‑1 (MQ=255)
                                                                                   aaGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                        >  1:352347/1‑141 (MQ=255)
                                                                                     ggCAATTAATTTAATAATTATAGCAAAATCTCGGTTATGTTGCAATGTTGAATCTATAGTATAATCTTATTTGGAATTAGTCATCTCTGGTAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGt                                                      <  1:1092599/141‑1 (MQ=17)
                                                                                       cAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTTTAGCCGTTg                                                    >  2:149162/1‑141 (MQ=255)
                                                                                        aattaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTgg                                                   >  2:763741/1‑141 (MQ=255)
                                                                                        aattaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTgg                                                   <  1:726103/141‑1 (MQ=255)
                                                                                        aattaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTgg                                                   >  2:691752/1‑141 (MQ=255)
                                                                                        aattaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATAACTGTAGCCGTTgg                                                   >  1:546252/1‑141 (MQ=255)
                                                                                          ttaatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTAAAATAATAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGct                                                 <  2:58844/141‑1 (MQ=255)
                                                                                            aatttaatAATTATAGCAATATATAGGTTCTGTTGCAAAGTTGAATCTAGCGGATTGTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCTGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctac                                               <  1:752533/141‑1 (MQ=17)
                                                                                            aatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctac                                               >  1:281314/1‑141 (MQ=255)
                                                                                             atttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctact                                              <  1:758994/141‑1 (MQ=255)
                                                                                             atttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctact                                              <  1:99474/141‑1 (MQ=255)
                                                                                                       tataGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGata                                    <  1:893217/141‑1 (MQ=255)
                                                                                                       tataGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTAAAATTATAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGata                                    <  2:64635/141‑1 (MQ=255)
                                                                                                        ataGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGatat                                   <  1:197586/141‑1 (MQ=255)
                                                                                                        aaaGCAAAATATAGATTCTGTTGCAAAGATGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGatat                                   <  1:1068754/139‑1 (MQ=255)
                                                                                                          aGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGc                                 >  2:464188/1‑141 (MQ=255)
                                                                                                           acagttaggaGGTTCTGTTGCAAAGTTGAATGTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCa                                <  2:1103588/132‑1 (MQ=11)
                                                                                                            cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                               >  1:84477/9‑141 (MQ=11)
                                                                                                            cAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTACCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTAACACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                               >  2:357933/1‑141 (MQ=255)
                                                                                                            cAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                               >  1:965373/1‑141 (MQ=255)
                                                                                                             gtaaatgaGGTTCTGTTGCAAAGTTGAATTTAGAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAtt                              >  1:1096080/8‑141 (MQ=11)
                                                                                                              taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                             >  1:1038913/7‑141 (MQ=255)
                                                                                                              gttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                             <  2:799276/135‑1 (MQ=9)
                                                                                                              gttaggaGGTTCTGTTGAAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTGGGCTACGATCTAAGATATGCATTg                             <  2:508612/135‑1 (MQ=11)
                                                                                                              aaaTATAGGTTCTGTTGCAAATTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                             <  2:908064/141‑1 (MQ=255)
                                                                                                              aaaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                             <  1:708847/141‑1 (MQ=40)
                                                                                                               ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                            <  2:1022166/136‑1 (MQ=9)
                                                                                                               ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                            <  1:80318/136‑1 (MQ=2)
                                                                                                               ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                            <  2:1012725/136‑1 (MQ=2)
                                                                                                               aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                            >  2:564082/6‑141 (MQ=2)
                                                                                                                taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                          <  2:96869/137‑2 (MQ=11)
                                                                                                                aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                           >  2:553126/5‑141 (MQ=2)
                                                                                                                 atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                          <  1:563534/138‑1 (MQ=2)
                                                                                                                 aggaGGTTCTGTTGTAAAGTTGAATTTATAATATAATTGTAACAAAAAGGAGTCTTCTGTATGAACTATTTCGGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                          <  2:190663/138‑2 (MQ=11)
                                                                                                                 aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                          <  1:903370/138‑2 (MQ=2)
                                                                                                                 aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                          <  1:494634/138‑2 (MQ=0)
                                                                                                                  ggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                         >  1:161564/3‑139 (MQ=2)
                                                                                                                   taGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGCTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGCGTTa                        <  2:264793/141‑1 (MQ=25)
                                                                                                                   taGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTa                        <  1:343361/141‑1 (MQ=38)
                                                                                                                   gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                        >  2:249482/2‑141 (MQ=11)
                                                                                                                   gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                        >  1:579066/2‑141 (MQ=0)
                                                                                                                   gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                        >  2:833289/2‑141 (MQ=0)
                                                                                                                    agcgttagtcgCAAAGTTAAATCTATAATATAATTTTAACAAAAAGGAGTCATCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGAGGTTATCACTCTAACTGTTGGCTACTATCTAAGAAATGCATTGAATTAt                       <  2:851746/131‑1 (MQ=11)
                                                                                                                    aGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAGAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTAt                       >  2:1040065/1‑141 (MQ=0)
                                                                                                                    aGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgcttt                       >  2:207376/1‑137 (MQ=0)
                                                                                                                    aGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTAt                       >  2:930683/1‑141 (MQ=2)
                                                                                                                    aGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTAt                       >  1:964315/1‑141 (MQ=35)
                                                                                                                    aGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTAt                       >  2:26866/1‑141 (MQ=35)
                                                                                                                    aGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATATAAGATATGCATTGAGTTAt                       >  2:510857/1‑141 (MQ=25)
                                                                                                                    aGGTTCTGTTGCAAAATTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTAt                       >  1:819755/1‑141 (MQ=11)
                                                                                                                           tttGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGata                <  1:245162/140‑1 (MQ=25)
                                                                                                                           gTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGata                <  1:833324/141‑1 (MQ=35)
                                                                                                                           gTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGata                <  1:631402/141‑1 (MQ=35)
                                                                                                                              tcaaatgtGAATTCATAATATAATCTTAACAAAAAGGAGTCTTCTGTAGGAACTACTGCAGATATAAACAATTTAACAAGTATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGatatat             <  1:314337/134‑1 (MQ=11)
                                                                                                                              gCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCACTGAGTTATCGCGatatct             <  2:1097948/141‑3 (MQ=19)
                                                                                                                              gCAAAGTTGAATATATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTAGGCTACTATCTAAGATATGCATTGAGCTATCGTGatatat             >  1:782287/1‑141 (MQ=255)
                                                                                                                              gCAAAGGTCAATGTAAAATATAATTTTAACAAAACATAGACATGTGTATGACTTGAATGTTAGATATACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGatatat             >  2:502922/1‑141 (MQ=2)
                                                                                                                               cAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc            >  2:972953/1‑141 (MQ=34)
                                                                                                                                aaaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCt           >  2:979393/1‑141 (MQ=35)
                                                                                                                                     tGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAat      >  1:161672/1‑141 (MQ=35)
                                                                                                                                     tGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAat      >  1:1078885/1‑141 (MQ=35)
                                                                                                                                     tGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAat      >  1:1006344/1‑141 (MQ=35)
                                                                                                                                      gAATCTATAGTATAATTTTAACAAAAAGGAGTCATCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGGAata     <  1:750572/141‑1 (MQ=21)
                                                                                                                                       aaTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGGGATATATCTGAAatat    >  2:356053/1‑141 (MQ=25)
                                                                                                                                         tCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTa  >  1:401708/1‑141 (MQ=33)
                                                                                                                                         tCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTAGTTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTa  >  1:1037362/1‑141 (MQ=21)
                                                                                                                                          |                                                                                                                                           
AATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTA  >  CP000730/76042‑76319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: