Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 620,399 T→A 9.0% V321D (GTC→GAC)  sdrE → Ser‑Asp rich fibrinogen/bone sialoprotein‑binding protein SdrE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730620,3990TA 9.0% 166.3 / 2.2 69V321D (GTC→GAC) sdrESer‑Asp rich fibrinogen/bone sialoprotein‑binding protein SdrE
Reads supporting (aligned to +/- strand):  ref base T (20/42);  new base A (2/5);  total (22/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGCTTCAAACAATGTAAATGATTTAATTAAAGTGACGAAGCAAACAATCAAAGTTGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGAACATTTGATAAAGCAACTAAGC  >  CP000730/620260‑620539
                                                                                                                                           |                                                                                                                                            
gTTGCTTCAAACAATGTAAATGATTTAATTAAAGTGACGAAGCAAACAATCAAAGTTGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTc                                                                                                                                             >  2:16351/1‑141 (MQ=255)
  tGCTTCAAACAATGTAAATCATCTAATTAAAGTGACGAAGCAAACAATCAAAGTTGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCaa                                                                                                                                           <  1:193889/141‑1 (MQ=255)
            attttAAAGGATTTAATTAAAGTGACGAAGCAAATAATCAAAGTTGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCgtt                                                                                                                                 <  2:77128/137‑3 (MQ=255)
            aaTGTAAATGATTTAATTAAAGTGACGAAGCAAACAATCAAAGTTGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGAt                                                                                                                                 <  1:1036860/141‑1 (MQ=255)
            aaTGCAAATCATTGAATTAAAGTGACGAAGCAAACAATCAAAGTTGGCTATGGTAAAGATAATGTGGCAACAGAGCAAAACGGTAAAGTCATTTAGTATATTACAGAGTTTTCAATTGACAATAAAGTCAAAAAAGGCGAt                                                                                                                                 <  2:124662/141‑1 (MQ=255)
            aaTGAAAATGATTTAATTAAAGAAAAGAAGCAAAAAATCAAAGTTGGCGATGGTAAAGATAATGGGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGAAAAAAAAAAAAAAAAAGGCGAt                                                                                                                                 <  2:522411/141‑1 (MQ=255)
              tGTAAATGATTTAATTAAAGTGACGAAGCAAACAATCAAAGTTGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATAc                                                                                                                               <  1:879701/141‑1 (MQ=255)
                 aaaTTATTTAAATAAAGAGACGAAGCAAACAATCAAATGTGAAGATGGTAAAGATAATGTCGAAGCAGTGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTTACAATAAAAAAAAAAAAGGCGATACAAt                                                                                                                            <  2:666561/141‑1 (MQ=255)
                   attaTTTAATTAAAGTGACGAAGCAAACAATCAAAGTTGGCGCTGGTAAAGATAATGTGTCAGCAGCGCATGACGGTAAAGATATCGAACATGATCCAGAGTTTAAAACTGACAATAAAGTAAAAAAAGGCGATACAATGa                                                                                                                          <  2:692176/138‑1 (MQ=255)
                    gtaTTTAATACTAGTGACGCAGCAAGTAATCAAAGGTTGCGATGGTAAAGATAAAGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATGTACAATAAAGTCAAAAAAGGCGATACAATggc                                                                                                                         <  2:230853/139‑3 (MQ=255)
                       tttAATTAAAGTGACGAATCAAACAATCAGAGGTGCGGATTGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAAGAACGTCAAAGAAGGAGATACTAttgccac                                                                                                                      <  2:775000/141‑7 (MQ=255)
                                    aCGAAGCAAACAATCAAAGTTGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAg                                                                                                         >  1:102842/1‑141 (MQ=255)
                                     cGAAGCAAACAATCAAAGTTGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGa                                                                                                        <  1:16351/141‑1 (MQ=255)
                                     cGAAGCAAACAATCAAAGTGGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGa                                                                                                        <  1:602704/141‑1 (MQ=255)
                                        aGCAAACAATCAAAGTTGGCGATGGTAAAGATAATGTGGCAGCAACGCATGACGGTAAAGATATAGAAAATAAAACAAAATATACAATTGACACTAAAAACAAAAAAGACGACAAAATTACGATAAATGCTGATAaaaaaa                                                                                                     >  2:1042715/1‑139 (MQ=255)
                                          cAAACAATCAAAGTTGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTa                                                                                                   >  1:37345/1‑141 (MQ=255)
                                          cAAACAATCAAAGTTGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTa                                                                                                   <  1:651184/141‑1 (MQ=255)
                                          cAAACAATCAAAGTTGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGAGATTGAATATGATACAGAGTTTACAGTTGACAATAAAGTCAAAAAAGGCGATAAGATGACGCTTAATTATGATCAAAATGTa                                                                                                   >  1:228053/1‑141 (MQ=255)
                                            atcAATCAAAGTTGGCGATGGTAAAGATATTGTGGCAGCAGCCCATGACGGTAAAGATATCGAATATGATACAGAGCTTACAATTCACATCATTTTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAAt                                                                                                 <  2:62691/139‑1 (MQ=255)
                                               aaTCAAAGTTGGCGATGGTAAAGGTGGGGGGGCGGCAGCGCATGACGGGAAAGATATTGAATATGATACAGAGTTGACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTcc                                                                                              <  2:1003510/141‑1 (MQ=255)
                                               aaTCAAAGTGGGCGATGGAAAAGATAAGGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAAAAAAAAAAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTcc                                                                                              <  2:987455/141‑1 (MQ=255)
                                                  cAAAGTTGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTc                                                                                           >  1:665507/1‑141 (MQ=255)
                                                  aaaaGGTGGCGATGGGAAAGATAATGGCGCAGCAGGGCACGACGGTAAAAATATTGAATATGATACAGAGTTTACAATTAAAAAAAAAAAAAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTc                                                                                           <  2:952515/140‑1 (MQ=255)
                                                    aaGTTGGCGATGGTAAAGAGAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGGTTACAATTGACAATAAAGACAAAAAAGGCGATACAATGACGATTGATGATGAGAAGAATGTAATTCCTTCga                                                                                         >  1:540334/1‑140 (MQ=255)
                                                     aGTTGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGa                                                                                        >  2:572989/1‑141 (MQ=255)
                                                     aGTTGGCGATGGAAAAGAAAATGTGGCAGCAGCGCATGACGGTAAAGATAATGAAAATGATACCGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATCCCTTCGGa                                                                                        <  2:953381/141‑1 (MQ=255)
                                                          gtgATGGTAAAGATAATGTGGCAGCAGCGCATGAAGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTaa                                                                                   <  1:739861/139‑1 (MQ=255)
                                                          gCGATGGTAAAGATAAGGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAACAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTaa                                                                                   <  1:36217/141‑1 (MQ=255)
                                                           cGATGGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAAc                                                                                  <  1:99789/141‑1 (MQ=255)
                                                             aTGGTAAAGATAATGTGGCAGCAGCGAATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAg                                                                                <  1:796475/141‑1 (MQ=255)
                                                                    aGATAATGTGGCAGCGGCGCATGACGGTAAAGATATTGAATATGATACGGAGTTTACAATTGACAATAAAGTCAAAAAAGCCGATACAAGGACGATTAAGTATGATAAGAATGTAATTCCTTCGTATTTAACAGATaaaaa                                                                         >  2:335951/1‑141 (MQ=255)
                                                                       tAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAAAAGATAAAAAtta                                                                      >  1:142369/1‑139 (MQ=255)
                                                                                agcagcGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGa                                                             <  2:72817/141‑1 (MQ=255)
                                                                                agcagcGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGa                                                             <  1:595212/141‑1 (MQ=255)
                                                                                agcagcGCATGACGGAAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATTACTATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGa                                                             <  1:42868/141‑1 (MQ=255)
                                                                                 gcagcGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGGCAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTACTTGGGATTTAACAGGTAAAAATGATCCTCTCGat                                                            >  1:350976/1‑141 (MQ=255)
                                                                                   agcGCATGACGGTAAAGATATCGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGatat                                                          <  1:298836/141‑1 (MQ=255)
                                                                                   agcGCATGACGGAAAAGAGATTGAATCTGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTTGGATTTAACAGATAAAAATGATCCTATCGatat                                                          <  2:1021001/141‑1 (MQ=255)
                                                                                          aaccgTAAAAATATAGAATATGATAAACAGTTTACAAATGAAAAAAAAAAAAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGAt                                                   <  2:609935/137‑1 (MQ=255)
                                                                                                 aaGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAg                                            >  1:998130/1‑141 (MQ=255)
                                                                                                 aaGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAg                                            >  1:491694/1‑141 (MQ=255)
                                                                                                   gATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATGAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGTTCCTATCGATATTACTGATCCATCAGga                                          >  1:767441/1‑141 (MQ=255)
                                                                                                      atTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGagag                                       <  2:350135/141‑1 (MQ=255)
                                                                                                      atTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGagag                                       <  1:126829/141‑1 (MQ=255)
                                                                                                      atTGAATACGGTACAGAGTTTTCAATGGACAATAAAGTGAAAAAAGGGCATACAATGACGATTAATTATGATAAGAATGTTATTCCTTCGGAAAAAAAAAAAAAAAATGATCCTATCGATATTACTGATCCATCAGGagag                                       <  2:143379/141‑1 (MQ=255)
                                                                                                      agtGAATATGATACAGATTTTACAATTGAAAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGagag                                       <  2:636335/139‑1 (MQ=255)
                                                                                                        tGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCTTATCGATATTACTGATCCATCAGGAGAGGt                                     <  2:672494/141‑1 (MQ=255)
                                                                                                          aaTAGGATACAGAGTTTACAATTTACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACATATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCa                                   <  2:876077/141‑1 (MQ=255)
                                                                                                            taagtaaCAGATTTAACAATTGACAGTAAAGTCAAAAAAGGCTATACAATGACGATTGATTGTGATAAGAATGTAATTCCTTCGGAGTTAACAGATAAAAATGATCCTATCGATATTACTTATCCATCAGGAGAGGTCAtt                                 <  2:482941/135‑1 (MQ=255)
                                                                                                                aTACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAAGTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCAATCGATATTACTGATCCATCAGGAGAGGTCATTGcga                             >  2:829551/1‑139 (MQ=255)
                                                                                                                    agagTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAgg                         <  1:957212/141‑1 (MQ=255)
                                                                                                                    agagTGTACAATTGAAAATAAAGTCAAAAAAGGCGATACAATGACGTTTAACTAAGTTTATAATGTAATTCCTTCGGATTTAACAGATAAAAATGTTCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAgg                         <  1:857293/141‑1 (MQ=255)
                                                                                                                           aCAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGCGCCATCAGGCAGGGTCCTTACTGAAGGAACAttt                  >  1:1085140/1‑141 (MQ=255)
                                                                                                                            cAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTGAAGGAACATTTg                 <  1:572989/141‑1 (MQ=255)
                                                                                                                            cAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGAACATTTg                 >  1:282258/1‑141 (MQ=255)
                                                                                                                            aaaTTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGAACATTTg                 <  1:607642/140‑1 (MQ=255)
                                                                                                                             aaTTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGAACATTTGa                <  1:602474/141‑1 (MQ=255)
                                                                                                                             aaTTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAACTATGAAAAGAATGTAATTCCGTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGAACATTTGa                <  1:52394/141‑1 (MQ=255)
                                                                                                                                tGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATACGAATGTAATTCCTCCGGACTTAACCGATAAATTTGATCCCATCGAGATTACTGATCCATCAGGAGAGTTCATTGCTAAAGGAACAATTGATaa             >  1:42679/1‑141 (MQ=255)
                                                                                                                                tGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGAACATTTGATaa             <  2:143744/141‑1 (MQ=255)
                                                                                                                                tGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGAACATTTGATaa             >  1:882411/1‑141 (MQ=255)
                                                                                                                                tGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGAACATTTGATaa             >  1:512412/1‑141 (MQ=255)
                                                                                                                                       aaaGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGAACATTTGATAAAGCAACt      >  2:851187/1‑141 (MQ=255)
                                                                                                                                         aGTCAAAAAATGCGACACAAGGACGATTAATTATGATACGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTTATCCATCAGGAGAGGTTATTGCTAAAGGAACATTTGATAAAGCAACTaa    <  2:697856/141‑1 (MQ=255)
                                                                                                                                         aGTCAAAAAAGGCGATACAATGACGATTAATTATGGTAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGAACATTTGATAAAGCAACTaa    <  1:925746/141‑1 (MQ=255)
                                                                                                                                         aGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGAACATTTGATAAAGCAACTaa    <  1:712480/141‑1 (MQ=255)
                                                                                                                                          gTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTCTAAGGTCAGTTGATAAAGCAACTAAg   <  1:536113/141‑1 (MQ=255)
                                                                                                                                          gTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGAACATTTGATAAAGCAACTAAg   <  1:538307/141‑1 (MQ=255)
                                                                                                                                          gTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAAAAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGAACCTTTGATAAAACaaaaaag   >  2:415987/1‑136 (MQ=255)
                                                                                                                                           tCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGAACATTTGATAAAGCAACTAAGc  <  1:15511/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
GTTGCTTCAAACAATGTAAATGATTTAATTAAAGTGACGAAGCAAACAATCAAAGTTGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGAACATTTGATAAAGCAACTAAGC  >  CP000730/620260‑620539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: