Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,742,872	A→G	5.0%	V292A (GTG→GCG)	relA2 ←	GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,742,872	0	A	G	5.0%	222.4 / 2.2	80	V292A (GTG→GCG)	relA2	GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base A (41/34);  new base G (2/2);  total (44/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

CGTTCGTATTTGGATTTCGAGCGGGTCTCCATTTGGGCCTACTACTGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTGTAAATATGTTTAGGTCTACCATTT  >  CP000730/1742739‑1743012                                                                                                                                      |                                                                                                                                             ctttCGTATTTGTGTTTCAGGCGGGTCTCCATTTGGGCCTACTACTGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGCTTTCCATAACGTATGCACCAAccc                                                                                                                                       <  2:623938/139‑1 (MQ=255)          ttGGATTTCGAGCGGGTCTCCATTTGGGCCTACTACTGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGGTTCCATAACGTATGCACCAACTCAAGTATCGc                                                                                                                              <  2:458717/141‑1 (MQ=255)          ttGGATTCCGCGCGGGTCTCCATTTCAGCCTACTACTGTAGTATGCAATGCGTGACACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGc                                                                                                                              <  1:241750/141‑1 (MQ=255)          ttGGATGTCGTGCGGGTCCCCATTTGGGCCTACTACTGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGc                                                                                                                              <  2:323572/141‑1 (MQ=255)               tttCGAGCGGGTCTCCATTTGGGCCTACTACTGTAGTATGCAATGACTGATACAAATTGTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCCCAAAAc                                                                                                                         >  1:261911/1‑141 (MQ=255)                ttCGAGCGGGTCTCCATTTGGGCCTACTACTGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACa                                                                                                                        >  1:23996/1‑141 (MQ=255)                   gAGCGGGTCTCCATTTGGGCCTACTACTGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTGCATAACGTATGCCCCAACCCGAGTATCGCATAACAATc                                                                                                                     >  2:334526/1‑141 (MQ=255)                   gAGCGGGTCTCCATTTGGGCCTACTACTGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACaaca                                                                                                                     >  2:980243/1‑139 (MQ=255)                     gCGGGTCTCCATTTGGGCCTACTACTGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCACGTATCGCATAAAAATCAt                                                                                                                   >  1:235031/1‑141 (MQ=255)                     gCGGGTCTCCATTTGGGCCTACTACTGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCAt                                                                                                                   >  1:819166/1‑141 (MQ=255)                        ggTCTCCATTTGGGCCTACTACTGTAGTATGCAATGACTGATACGAATTTTGTTTAGGCATTGCACTATAATCTTTACAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTaa                                                                                                                <  2:320681/141‑1 (MQ=255)                         gTCTCCATTTGGGCCTACTACTGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTaaa                                                                                                               >  1:924289/1‑140 (MQ=255)                              cATTTGGGCCTACTACTGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAAt                                                                                                          <  2:1102558/141‑1 (MQ=255)                                   gggCCTACTACTGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACa                                                                                                     >  1:996545/1‑141 (MQ=255)                                   gggCCTACTACTGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACa                                                                                                     >  1:439692/1‑141 (MQ=255)                                    ggCCTACTACTGTAGTATGCAATGACTGATACAAATTTTGTTTAGTCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACaa                                                                                                    >  1:425216/1‑141 (MQ=255)                                    ggCCTACTACTGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACaa                                                                                                    >  1:693123/1‑141 (MQ=255)                                            actTTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGt                                                                                            <  2:1078441/141‑1 (MQ=255)                                            actGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAACATAATCCTCAAAACGTCCTGGCATCGGTTTCCAGAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGt                                                                                            <  2:749462/141‑1 (MQ=255)                                              tGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATTCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTAt                                                                                          <  2:617208/141‑1 (MQ=255)                                              tGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTAt                                                                                          <  2:1010970/141‑1 (MQ=255)                                               gtagtaTGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATc                                                                                         >  1:351462/1‑141 (MQ=255)                                                      gCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCCCATAACAATAATTAATAGAATTGAAAATAACCGGTATCGCcaaca                                                                                  >  2:474241/1‑141 (MQ=255)                                                        aaTGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACaaa                                                                                <  2:124085/141‑1 (MQ=255)                                                        aaTGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACaaa                                                                                <  2:829625/141‑1 (MQ=255)                                                            aCTGATACAAATTTTGTTTAGGCATTGAAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCaa                                                                            <  2:380661/141‑1 (MQ=255)                                                             cTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATCACACGTATCGCCAACAAATCaaa                                                                           >  2:104151/1‑141 (MQ=255)                                                                aTACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAAAAATAATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAAt                                                                        <  2:281013/141‑1 (MQ=255)                                                                   cAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCGTAACAATGATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTg                                                                     >  1:816317/1‑141 (MQ=255)                                                                   cAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTg                                                                     >  1:575844/1‑141 (MQ=255)                                                                    aaaTTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGa                                                                    >  1:948449/1‑141 (MQ=255)                                                                          tttttCGGGATTGAAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGTACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAAt                                                              <  2:325145/139‑1 (MQ=255)                                                                          tttttAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAAt                                                              <  2:971602/139‑1 (MQ=255)                                                                          tGTTTAGGCATTGCAATATAATCTTTAAACCGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAAt                                                              <  2:974356/141‑1 (MQ=255)                                                                          tGTTTAGGCATTGCAATATAATCCTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAAt                                                              <  2:184563/141‑1 (MQ=255)                                                                                ggCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCGCCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGtttt                                                        >  2:850405/1‑141 (MQ=255)                                                                                ggCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCGCCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGtttt                                                        >  1:104821/1‑141 (MQ=255)                                                                                ggCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGtttt                                                        >  1:988914/1‑141 (MQ=255)                                                                                 gCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTACCATAACCTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGttttt                                                       >  2:690689/1‑141 (MQ=255)                                                                                     ggCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCGAAAATTTGATCAAATTGttttttttt                                                   >  2:459589/2‑138 (MQ=255)                                                                                        aaTATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCAGCGACCCAAGTATCGCGTAACAATCACAAATAGAATTGACAATAACACGTATGGCCAACAAATGAAAAATGTGATCAAATGGttatttgggcct                                                >  2:384576/1‑135 (MQ=255)                                                                                        aaTATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGAAAAAAAATAAAAAATTTGATCAAATTGTTTTTTCTGCtt                                                <  2:23996/141‑1 (MQ=255)                                                                                          ttaaaTCTTTAAAACCGCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTtca                                              <  2:97937/138‑1 (MQ=255)                                                                                          tataGTCTTTCAAAGGTATTTGCATCGGTTTCCTTAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTtca                                              <  1:541810/141‑1 (MQ=255)                                                                                          tataATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTtca                                              >  1:283339/1‑141 (MQ=255)                                                                                          tataATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTtca                                              >  1:1066935/1‑141 (MQ=255)                                                                                           ataATCTGTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTtcat                                             <  2:832598/141‑1 (MQ=255)                                                                                            taATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACGAATCAAAAATTTGTTCAAATTGTTTTTTCTGCTtcatc                                            <  1:708567/141‑1 (MQ=255)                                                                                            taATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTtcatc                                            <  2:231052/141‑1 (MQ=255)                                                                                            taATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTtcatc                                            <  2:347022/141‑1 (MQ=255)                                                                                            taATCTTGTACACGTCCTGGCATCGGTTTCCATAACGGATGCACCATTCCTAGTATCGCAGTACAATCAGTAATAGATTTGACAATTACTCGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTtcatc                                            <  2:485412/141‑1 (MQ=255)                                                                                              aTCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGAATAACAATCATTTATAGAATTGACGATTACTCTTATCGCCAACAGATCAAAAATTTGATCAAATTGTTTTTTCTGCTTcatcat                                          <  2:369662/141‑1 (MQ=255)                                                                                              aTCTTTAAAACCTCCTTGCATCGGTTTCCATAACGTATGCACAAACCCAAGTATCGCATAACAATCATTAATAGAATTGATAATAACACGTATCGCCAACAACTCAAAAATTTGATCAAATTGTTTTTTCTGCTTcatcat                                          <  2:451121/141‑1 (MQ=255)                                                                                                       aCGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATa                                 >  2:1031570/1‑141 (MQ=255)                                                                                                       aCGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATa                                 >  2:948980/1‑141 (MQ=255)                                                                                                       aCGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATa                                 >  1:956270/1‑141 (MQ=255)                                                                                                         gTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTCTAGCATAACAATCATTAATAGAATTGACAATAACACGGATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTACTTCATCATTTTCCCATaaa                               >  1:532993/1‑141 (MQ=255)                                                                                                          tCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAAACATAAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTACATCAAATTGTTTTTTCTGCCTCATCATTTTCCGATaccc                              >  1:614321/1‑138 (MQ=255)                                                                                                            cTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATaa                            >  1:792899/1‑140 (MQ=255)                                                                                                            cTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATAc                            <  2:239375/141‑1 (MQ=255)                                                                                                             tGGCATCTGTTTCCATAACGTATGCACCAACCCCAGTATCGCATAACAATCATTAATATAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACt                           <  2:395386/141‑1 (MQ=255)                                                                                                             tGGCATCGGTTTCCATAACGTATGCACCAACCCCAGTAGCGCACTACAATTATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACt                           <  2:312189/141‑1 (MQ=255)                                                                                                             tGGCATCGGTTTCCATAACGTATGCACCAACCAAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACt                           >  1:156088/1‑141 (MQ=255)                                                                                                              ggCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTg                          >  1:967514/1‑141 (MQ=255)                                                                                                              ggCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTg                          >  1:977603/1‑141 (MQ=255)                                                                                                              ggCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAAAACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTg                          >  1:43975/1‑141 (MQ=255)                                                                                                                  tCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAGCAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTGTaaa                      <  2:561887/141‑1 (MQ=255)                                                                                                                  ccGGTTTCCATTACGGACGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTAGCGCCAACAAATCAAATATTTGATAAAATTTTTTTTTCTGCTTCATCATTTTCCGATAAATACTGTaaa                      <  1:1033380/140‑1 (MQ=255)                                                                                                                      tttCCATAACGTATGCGCCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTGTAAATATg                  <  1:850405/141‑1 (MQ=255)                                                                                                                      tttCCATAACGTATGCGCCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTGTAAATATg                  <  2:104821/141‑1 (MQ=255)                                                                                                                          cATAACTTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAAGTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTGTAAATATGTTTa              <  2:622916/141‑1 (MQ=255)                                                                                                                          cATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTGTAAATATGTTTa              >  1:37548/1‑141 (MQ=255)                                                                                                                              aCGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTGTAAATATGTTTAGGTc          >  1:1010664/1‑141 (MQ=255)                                                                                                                               cGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTGTAAATATGTTTATGTCt         >  1:581236/1‑141 (MQ=255)                                                                                                                               cGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTGTAAATATGTTTAGGTCt         >  1:357437/1‑141 (MQ=255)                                                                                                                               cGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTGTAAATATGTTTAGGTCt         >  2:738097/1‑141 (MQ=255)                                                                                                                                  aTGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTGTAAATCTTTTTAGGTTTAca      >  1:515076/1‑140 (MQ=255)                                                                                                                                   tGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGGCAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTGTAAATATGTTTAGGTCTAcct     >  1:863594/1‑140 (MQ=255)                                                                                                                                   tGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCGTCATCATTTTCCGATAAATACTGTAAATATGTTTAGGTCTACCa     >  1:144657/1‑141 (MQ=255)                                                                                                                                    gCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTGTAAATATGTTTAGGTCTACCAt    >  1:408577/1‑141 (MQ=255)                                                                                                                                      aCCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTGTAAATATGTTTAGGTCTACCAttt  <  1:308873/141‑1 (MQ=255)                                                                                                                                      |                                                                                                                                             CGTTCGTATTTGGATTTCGAGCGGGTCTCCATTTGGGCCTACTACTGTAGTATGCAATGACTGATACAAATTTTGTTTAGGCATTGCAATATAATCTTTAAAACGTCCTGGCATCGGTTTCCATAACGTATGCACCAACCCAAGTATCGCATAACAATCATTAATAGAATTGACAATAACACGTATCGCCAACAAATCAAAAATTTGATCAAATTGTTTTTTCTGCTTCATCATTTTCCGATAAATACTGTAAATATGTTTAGGTCTACCATTT  >  CP000730/1742739‑1743012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑