Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,884,975 T→A 5.9% N82Y (AAT→TAT)  fmtB1 ← cell surface anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,884,9750TA 5.9% 210.3 / 2.8 67N82Y (AAT→TAT) fmtB1cell surface anchored protein
Reads supporting (aligned to +/- strand):  ref base T (33/30);  new base A (2/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

CTCAGTTGCTGTTGTATTGCCATCTGTATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTTGTGAATTTACAGGTGTTGCTTGATTAGTATCGCTTTGTACATTATTATCCGTA  >  CP000730/1884838‑1885102
                                                                                                                                         |                                                                                                                                
ctcAGTTGCTGTTGTATTGCCATCTGTATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGgt                                                                                                                               <  1:858979/141‑1 (MQ=255)
ctcAGTTGCTGTTGTATTGCCATCTGTATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGgt                                                                                                                               <  2:285621/141‑1 (MQ=255)
  cAGTTGCTGTTGTATTGCCATCTGTATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGgttg                                                                                                                             >  1:711854/1‑141 (MQ=255)
    gTTGCTGTTGTATTGCCCTCTGAGTGAAGATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGgttgtt                                                                                                                           >  2:237082/1‑141 (MQ=255)
    gTTGCTGTTGTATTGCCATCTGTATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGgttgtt                                                                                                                           >  2:515952/1‑141 (MQ=255)
    gTTGCTGTTGTATTGCCATCTGTATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGgttgtt                                                                                                                           >  1:685694/1‑141 (MQ=255)
    gTTGCTGTTGTATTGCCATATGCATATTTGTTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGgttgtt                                                                                                                           >  2:237246/1‑141 (MQ=255)
      tGCTGTTGTATTGCCATCTGTATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGc                                                                                                                         <  2:143876/141‑1 (MQ=255)
         tgttgtATTGCCATCTGTATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAga                                                                                                                      <  1:938267/141‑1 (MQ=255)
            tgtATTGCCATCTGTATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAgattg                                                                                                                   <  2:768607/141‑1 (MQ=255)
            tgtATTGCCATCTGTATGATTATTGTTTTGTGCTGAAGGCGTACGTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAgattg                                                                                                                   <  2:742618/141‑1 (MQ=255)
                 tGCCATCTGTATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATAGGTTGTTGCAGATTGATTAg                                                                                                             >  1:918318/1‑141 (MQ=255)
                 tGCCATCTGTATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATAGGTTGTTGCAGATTGATTAg                                                                                                             >  1:539531/1‑141 (MQ=255)
                  gCCATCTGTATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAgg                                                                                                            >  2:571909/1‑141 (MQ=255)
                  gCCATCTGTATGATTAGTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAgg                                                                                                            >  1:655135/1‑141 (MQ=255)
                   ccATCTGTATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGgt                                                                                                           >  1:63255/1‑141 (MQ=255)
                     aTCTGTATGATTATTGTTTTGTTCTGAAGGCGTACTTGATTGCACTGATTTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGgtgt                                                                                                         <  2:296939/141‑1 (MQ=255)
                     aTCTGTATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGCTTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGgtgt                                                                                                         >  2:41421/1‑141 (MQ=255)
                       cTGTATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTAt                                                                                                       <  1:760592/141‑1 (MQ=255)
                        tGTATTATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTAtt                                                                                                      <  2:654891/141‑1 (MQ=255)
                        tGTAGGATTATTGTTGTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACCACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTAtt                                                                                                      <  2:567851/141‑1 (MQ=255)
                          tATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCt                                                                                                    >  1:362159/1‑141 (MQ=255)
                                   tctgttGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTAt                                                                                           <  1:451847/137‑1 (MQ=255)
                                   tGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTAt                                                                                           >  2:1176/1‑141 (MQ=255)
                                   tGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTAt                                                                                           <  2:838996/141‑1 (MQ=255)
                                   tGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTAt                                                                                           <  2:1084436/141‑1 (MQ=255)
                                   tGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTAt                                                                                           <  1:149327/141‑1 (MQ=255)
                                   tGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTAt                                                                                           <  1:164857/141‑1 (MQ=255)
                                    gctttGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATATACTATACTACCATTATTATGATTAACCAATGCTTCATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTAtt                                                                                          >  2:816351/3‑141 (MQ=255)
                                     ttttGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTAttt                                                                                         >  1:359729/1‑141 (MQ=255)
                                       ttcggcagAAGGTGTACTTGATTGCACTGATGGTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGACTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGc                                                                                       <  1:38356/134‑1 (MQ=255)
                                       ttGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGc                                                                                       <  1:88721/141‑1 (MQ=255)
                                       ttGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATATACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTACTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGc                                                                                       <  1:693095/141‑1 (MQ=255)
                                       ttGGGCGGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGc                                                                                       <  1:295622/141‑1 (MQ=255)
                                         gtgCGGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGTTTATGTTTATTCTGCGCACTATTTGCTa                                                                                     <  2:507058/141‑1 (MQ=255)
                                          tgCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTaa                                                                                    >  2:1022289/1‑141 (MQ=255)
                                          tgCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGAGTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTGAAGTGATTCTGGGAACTATTGGCTaa                                                                                    >  2:1110474/1‑141 (MQ=255)
                                             tGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATGATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAAcc                                                                                 >  1:403954/1‑141 (MQ=255)
                                                     tACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCtattat                                                                         >  1:1067842/1‑141 (MQ=255)
                                                     tACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCtattat                                                                         >  1:937718/1‑141 (MQ=255)
                                                     tACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCtattat                                                                         >  1:563255/1‑141 (MQ=255)
                                                     tACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCtattat                                                                         >  1:844537/1‑141 (MQ=255)
                                                     tACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCtattat                                                                         >  1:307744/1‑141 (MQ=255)
                                                      aCTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTTCCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTattatt                                                                        <  2:917184/141‑1 (MQ=255)
                                                        ttGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGCTTGATTAGGTGTATTCTGCGCACTATTTGCTAACCCTCTATTATTAg                                                                      >  1:545380/1‑141 (MQ=255)
                                                        ttGATTGCACTGATGATGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATATGCTAAACCTCTATTATTAg                                                                      >  1:818273/1‑141 (MQ=255)
                                                           aTTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCaa                                                                   >  1:612771/1‑141 (MQ=255)
                                                                     tGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATc                                                         <  1:261120/141‑1 (MQ=255)
                                                                         ggCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATAGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTtgt                                                     <  2:918318/141‑1 (MQ=255)
                                                                         ggCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATAGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTtgt                                                     <  2:539531/141‑1 (MQ=255)
                                                                             tAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTTGTGAAt                                                 <  1:1176/141‑1 (MQ=255)
                                                                              aGCTTGATTTTCTATTCTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTTGTGAAtt                                                <  2:420455/141‑1 (MQ=255)
                                                                                    aTTTACTACACTGCCAGTATTATGATTAACCAAGGCTTGATTCGTTGCTTGATTGGTTGTTGAAGACTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTTGTGAATTTACAgg                                          <  1:692474/141‑1 (MQ=255)
                                                                                         ctatactaCCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGCTGTGGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTCGACGTCTATTATTAGCAACATCGTTATCTTGTGAATTTACAGGTGTTg                                     >  1:921026/1‑141 (MQ=255)
                                                                                                  catttgtatGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTTGTGAATTTACAGGTGTTGCTTGATTAg                            <  2:380445/135‑1 (MQ=255)
                                                                                                   tttattaTGATTAAACATTGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTTGAATTGTCACAGGTGTTGCTTGATTAGt                           <  2:508310/140‑1 (MQ=255)
                                                                                                     tattatGATTAACTAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCCTTTATTAGCAACATCTTTATCTTGTGAATTTACAGGTGTTGCTTGATTAGTAt                         <  1:367414/141‑1 (MQ=255)
                                                                                                       ttatGATTAAGCAACGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTTGTGAATTTACAGGTGTTGCTTGATTAGTATCg                       >  2:749325/1‑141 (MQ=255)
                                                                                                               aaCCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTTGTGAATTTACAGGTGTTGCTTGATTAGTATCGCTTTGTAc               <  2:711854/141‑1 (MQ=255)
                                                                                                                      gCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTTGTGAATTTACAGGTGTTGCTTGATTAGTATCGCTTTGTACAttatta        >  2:1033268/1‑141 (MQ=255)
                                                                                                                        ttGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTTGTGAATTTACAGGTGCTGCTTGATTAGTATCGCTTTGTACATTATTATc      <  2:547398/141‑1 (MQ=255)
                                                                                                                         tGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAGCCTCTATTATTAGCAACATCTTTATCTTGTGAATTTACAGGTGTTGCTTGATTAGTATCGCGTTGTACATTATTATcc     >  1:294626/1‑141 (MQ=255)
                                                                                                                         tGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTTGTGAATTTACAGGTGTTGCTTGATTAGTATCGCTTTGTACATTATTATcc     >  2:760869/1‑141 (MQ=255)
                                                                                                                         tGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTTGTGAATTTACAGGTGTTGCTTGATTAGTATCGCTTTGTACATTATTATcc     >  2:407462/1‑141 (MQ=255)
                                                                                                                         tGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTTGTGAATTTACAGGTGTTGCTTGATTAGTATCGCTTTGTACATTATTATcc     >  2:395260/1‑141 (MQ=255)
                                                                                                                         tGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTTGTGAATTTACAGGTGTTGATTGATTAGTATCGCTTTGTGCATTATTATcc     >  2:479297/1‑141 (MQ=255)
                                                                                                                            ttCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTTGTGAATTTACAGGTGTTGCTTGATTAGTATCGCTTTGTACATTATTATCCGTa  >  1:756703/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                
CTCAGTTGCTGTTGTATTGCCATCTGTATGATTATTGTTTTGTGCTGAAGGCGTACTTGATTGCACTGATGTTGGCGTAGCTTGATTTACTATACTACCATTATTATGATTAACCAATGCTTGATTCGTTGCTTGATTGGTTGTTGCAGATTGATTAGGTGTATTCTGCGCACTATTTGCTAAACCTCTATTATTAGCAACATCTTTATCTTGTGAATTTACAGGTGTTGCTTGATTAGTATCGCTTTGTACATTATTATCCGTA  >  CP000730/1884838‑1885102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: