Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,113,299 G→T 6.4% D38E (GAC→GAA USA300HOU_1981 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,113,2990GT 6.4% 701.8 / 6.4 233D38E (GAC→GAAUSA300HOU_1981hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (116/97);  new base T (17/3);  total (133/100)
Fisher's exact test for biased strand distribution p-value = 8.73e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 241 total aligned reads displayed.

CTTGTAGGATGTTAGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGCT  >  CP000730/2113159‑2113439
                                                                                                                                             |                                                                                                                                            
cTTGTAGGATGTTAGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGGAATTATTGTTTTTTCg                                                                                                                                              >  1:940478/1‑141 (MQ=255)
  tGTAGGATGTTAGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTc                                                                                                                                            >  1:1052008/1‑141 (MQ=255)
   gTAGGATGTTAGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAACTCTTCTGACACGAATACCTTTAAATCATGGAATTCTGAAATTATTGTTTTTTCGTCa                                                                                                                                           >  2:434734/1‑141 (MQ=255)
    tcggATGTTAGTCAGTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCAt                                                                                                                                          <  1:175870/139‑1 (MQ=255)
    tacgATGTTAGTCATTTTCCTTGTCCTCCTCATCAATTCCACCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCAt                                                                                                                                          <  2:987162/138‑1 (MQ=255)
      gtaTGTTAGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATgg                                                                                                                                        <  2:187967/139‑1 (MQ=255)
         tGTTAGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGtgg                                                                                                                                     >  1:327036/1‑139 (MQ=255)
           ttatctaTTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGAt                                                                                                                                   <  2:379366/135‑1 (MQ=255)
             aGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGt                                                                                                                                 >  1:966268/1‑141 (MQ=255)
              gTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTa                                                                                                                                >  2:635126/1‑141 (MQ=255)
              gTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTa                                                                                                                                >  2:797878/1‑141 (MQ=255)
                cagtttCCTGTTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGACTAACTTTAAAACATGATACGGGGGCATTATTGTTTTTTCGTCATGGTCGATGTaaa                                                                                                                              >  2:499544/4‑141 (MQ=255)
                cATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTaaa                                                                                                                              <  2:492377/141‑1 (MQ=255)
                cATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAAAGCTACAAAGACTTTTATATATTCCTACCATTGACACGAATACCTTAAAATCATGTAATTCTGTTATTATTGTTTTTTCGTCATGGTCGGGGTaaa                                                                                                                              >  1:808752/1‑141 (MQ=255)
                      ccTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTACATTTCTa                                                                                                                        >  2:973075/1‑141 (MQ=255)
                      ccTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTa                                                                                                                        >  1:68963/1‑141 (MQ=255)
                       cTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAAGGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATGCCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAGATTTCtgg                                                                                                                       >  2:598823/1‑139 (MQ=255)
                       cTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTACTTATTGTTTTTTCGTCATGGTCGATGTGAATCTATAg                                                                                                                       >  1:135740/1‑141 (MQ=255)
                       cTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAg                                                                                                                       <  1:623988/141‑1 (MQ=255)
                          ttcaccctcATCAATTCCAGCCAATTCATACATAACTCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGt                                                                                                                    <  2:740739/136‑1 (MQ=255)
                          gtacttctcATCATTCCCTGCCAATTCATACATATCGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGt                                                                                                                    <  2:1041991/135‑1 (MQ=255)
                           tcctccTCATCTATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTa                                                                                                                   <  2:1024632/141‑1 (MQ=255)
                           gcctcctCATCCATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTa                                                                                                                   <  1:192591/140‑1 (MQ=255)
                                   atcaATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGc                                                                                                           >  1:435802/1‑141 (MQ=255)
                                   atcaATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGc                                                                                                           <  1:403871/141‑1 (MQ=255)
                                     caATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTa                                                                                                         >  1:456933/1‑141 (MQ=255)
                                      aaTTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCCGCTaa                                                                                                        >  2:531362/1‑141 (MQ=255)
                                      aaTTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTaa                                                                                                        >  1:344442/1‑141 (MQ=255)
                                        ttCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCTTCTTTTTTGATGTAAATTTCTAGCGTACCATCAGCTAAAt                                                                                                      >  2:955227/1‑141 (MQ=255)
                                         tCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAAtt                                                                                                     <  2:161546/141‑1 (MQ=255)
                                           cAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTaa                                                                                                   <  2:1095287/141‑1 (MQ=255)
                                            cgCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTaaa                                                                                                  <  1:118236/140‑1 (MQ=255)
                                             gCCAATTCATACATCACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTaaaa                                                                                                 <  1:906568/141‑1 (MQ=255)
                                             gCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCGACTAAATTaaaa                                                                                                 >  1:653207/1‑141 (MQ=255)
                                                    catacataACGCTCTCCATACTTTTATCTTTTAAATCTACTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCCGCTACATTAAAAATAGCtt                                                                                          <  1:719574/141‑1 (MQ=255)
                                                       acataACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTAt                                                                                       >  1:87561/1‑141 (MQ=255)
                                                                tctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTCTCTTCActtt                                                                              >  2:84076/1‑141 (MQ=255)
                                                                tctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCGTTATCTTCTtttt                                                                              >  1:297138/1‑141 (MQ=255)
                                                                tctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAACGTACCATAAGCTAAATTAAAAATGGCTTTATCTTCAtttt                                                                              >  2:883980/1‑141 (MQ=255)
                                                                  tcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCt                                                                            >  1:482818/1‑141 (MQ=255)
                                                                   ccATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTTTTTTTTTTTGATGTAAATTTCTGGCGTACCGTCAGCTAAATTAAAATTAGCTTTATTTTAATTTTCtt                                                                           >  2:509553/1‑141 (MQ=255)
                                                                    cATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCTTTTTTTTTGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAATTGGCTTCATCTTCATTTTCttt                                                                          >  2:944165/1‑141 (MQ=255)
                                                                    cATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCttt                                                                          >  1:350783/1‑141 (MQ=255)
                                                                     aTACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCtttt                                                                         >  1:865033/1‑140 (MQ=255)
                                                                      tACTTTTATCTTTTAAATATTCTGAAACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTAGTCATGTTCGCTGTAAATTTATAGCGAACCATCAACTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGt                                                                        >  2:41683/1‑141 (MQ=255)
                                                                          tttATCTTTTAAATAGTCTGCATCGAATGCGTTTAAATTATGGAATTCTGTACTTATTTTTTTTTCGTCATGGTGGATGTCAATTTCTAGCGTACCATCATCTAAATTAAAAATAGCTTTATCTTCCTTTTCTTTGTATcc                                                                    <  2:867454/141‑1 (MQ=255)
                                                                                  ttAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCTTTGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTCCATTTTCTTTGTATCCTTTTTCtt                                                            >  2:903223/1‑141 (MQ=255)
                                                                                  ttAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCtt                                                            >  1:200614/1‑141 (MQ=255)
                                                                                  ttAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTGATCTTCCTTTTCTTTGTATCCTTTTTCtt                                                            >  1:385910/1‑141 (MQ=255)
                                                                                  ttAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGGCCTGGTCGATGTAAATTTCTCGCGTACCATCAGCTACATTAAAAATAGCTTTATCTTCATTTTCTTTGTTTCCGTTTTCtt                                                            >  1:58306/1‑141 (MQ=255)
                                                                                   tAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCttt                                                           <  1:880320/141‑1 (MQ=255)
                                                                                   tAAATCTTCTGACACGAATACCTTTAAATCAGGGAATTCTAATTCACCTACTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCttt                                                           <  1:520905/141‑1 (MQ=255)
                                                                                    aaaTCTTCTGACACGGATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTGTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCtttt                                                          <  1:900801/141‑1 (MQ=255)
                                                                                    aaaTCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTTTTTTTTTTTTTTGTAAATTTCTAGCTTACCATCAGCTAAATTAGACTTAGCTTTTCCTTCGTTTTCTTTGTATCTtttttttttt                                                          >  2:613354/1‑141 (MQ=255)
                                                                                    aaaTCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGAACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCtttt                                                          <  1:664744/141‑1 (MQ=255)
                                                                                       tcttctGACACGGATACCTTTTCATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAAt                                                       <  2:302517/141‑1 (MQ=255)
                                                                                         ttctGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAAttt                                                     >  1:757999/1‑141 (MQ=255)
                                                                                          tctGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCTTTTTTTTTTATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCCTTTTCTTTGTATCCTTTTTTTTTTTAtttt                                                    >  2:939743/1‑140 (MQ=255)
                                                                                          tctGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGCTGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCGTTTTCTTTGTATCCTTTTTCTTTTTAtttt                                                    >  2:828753/1‑140 (MQ=255)
                                                                                          tctGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTTAtttt                                                    >  2:551235/1‑140 (MQ=255)
                                                                                           ctGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATttct                                                   <  1:141997/141‑1 (MQ=255)
                                                                                           ctGACACGAATACCTCTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATttct                                                   >  1:553765/1‑141 (MQ=255)
                                                                                             gACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTTTTTTTTTTCTATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATttcttc                                                 >  2:281210/1‑141 (MQ=255)
                                                                                             gACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATttcttc                                                 >  1:785700/1‑141 (MQ=255)
                                                                                              acacGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTACTTTCTTCa                                                >  1:83238/1‑141 (MQ=255)
                                                                                              acacGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCa                                                >  1:991390/1‑141 (MQ=255)
                                                                                              acacGAATACCTTTAAATCATGGAATTCGGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCa                                                >  1:578297/1‑141 (MQ=255)
                                                                                               cacGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAGTTAAAAATAGCTTTATCTTCATTTTCTTTGTTTCCTTTTTCTTTTATTTTGTTCaa                                               >  2:107887/1‑141 (MQ=255)
                                                                                                acGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTTTTTTTTTTTTATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGGTTTTTCTTCTTTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCatt                                              >  2:56532/1‑139 (MQ=255)
                                                                                                 cGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTATTTTCTTCCAAg                                             >  2:34281/1‑141 (MQ=255)
                                                                                                 cGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTAGCCTTTTTCTTTTAATTGCTTCaaaa                                             >  1:499792/1‑140 (MQ=255)
                                                                                                  gAATACCTTTAAATCATGGAGTTCTGTAATTATTTTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGa                                            <  2:725770/141‑1 (MQ=255)
                                                                                                      aCCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTTTTCAAAGATTCa                                        >  2:13921/1‑141 (MQ=255)
                                                                                                       ccTTTAAATCATGGAATCCTGACGTTGTTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTACATTGAACATAGCTTTATCTTCATGTTCTTCGTATCCATTTTCTTTTAAATTCTTCGAAGATTAAt                                       >  2:785331/1‑141 (MQ=255)
                                                                                                          ttAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTaa                                    >  2:366349/1‑141 (MQ=255)
                                                                                                             aaTCATGGAATTCTGTAATTATTGTTTTTTTTTTTTTTTCGATGTAAATTTCTAGCTTACCATCAGCTAAATTAAAATTAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGTTTCATTAattt                                 >  2:471590/1‑141 (MQ=255)
                                                                                                             aaTCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAattt                                 <  2:456933/141‑1 (MQ=255)
                                                                                                              aTCATGCAATTCTGTAATTTTTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAatttt                                <  2:149945/141‑1 (MQ=255)
                                                                                                               tCATGGAATTCTGTAATTATTGTTTTTTTTTTTTTTTTTATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTTTTTTAATTTCTTCAAAGTTTTATTAtttttt                               >  2:680364/1‑140 (MQ=255)
                                                                                                                 aTGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAGGATTCATTAtttttcat                             >  1:42127/1‑141 (MQ=255)
                                                                                                                  tGGAATTCTGTAATTATTGTTTTTTTTTTATTTTCGAAGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGATTTATCTTCATTTTCTTTCTATCCCTTTTCTTTTAATTTCTTCAAAGATTCATTAattttcatt                            >  2:268995/1‑141 (MQ=255)
                                                                                                                  tGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTCATTTCTTCAAAGACTCAttctttttcttt                            >  1:321690/1‑141 (MQ=255)
                                                                                                                  tGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAattttcatt                            >  2:506039/1‑141 (MQ=255)
                                                                                                                  tGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAattttcatt                            >  1:935942/1‑141 (MQ=255)
                                                                                                                  tGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAAATTCATTAattttcatt                            >  2:75854/1‑141 (MQ=255)
                                                                                                                      aTTCTGTAATTATTGTTTTTTTTTTTTTTTTGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAATGATTTATTTatttttattttct                        >  2:677443/1‑140 (MQ=255)
                                                                                                                       ttCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCt                       <  1:609144/141‑1 (MQ=255)
                                                                                                                        tCTGTAATTCTTGTTTTTTCGCCATGGGCGATGTAAAGTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTg                      <  1:310027/141‑1 (MQ=255)
                                                                                                                        tCTGTAATTATTTGTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTg                      <  2:20146/141‑1 (MQ=255)
                                                                                                                         cTGTATTTATTGTTTTTTCGTCATGGTCGATGTCAATTTCTAGCGTCCCATCAGCTAAATTAAAAATAGCTTTATCTTCGTTTTCTTTGTGTCCTTTTTCTTTTAATTTCTTCGAAGATTCATTAattttcattttattgt                     >  1:267270/1‑136 (MQ=255)
                                                                                                                            tAATTATTGTTTTTTTTTTATTTTTTATGTAAATTACTAGCGTACCATCAGCTAAATTAGAATTAGCTTTATCTTCATTTTCTTTGTATCCTTTTTTTTTTAATTTACTCAATGATTCATTACTTTTCATTTTCCTGTtcc                  >  2:618784/1‑141 (MQ=255)
                                                                                                                            tAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAACATCGCTTTATCTACTTTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTATTTTTCATTTTCTTGtttc                  >  2:393371/1‑139 (MQ=255)
                                                                                                                            tAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTtcc                  >  2:652386/1‑141 (MQ=255)
                                                                                                                                tattGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTcctcct              <  1:635126/141‑1 (MQ=255)
                                                                                                                                tattGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTcctcct              <  1:881674/141‑1 (MQ=255)
                                                                                                                                    tGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCat           <  2:872608/141‑1 (MQ=255)
                                                                                                                                      ttttttCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCatat         <  1:1093875/141‑1 (MQ=255)
                                                                                                                                       tttttCTTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTACATTAAAAATAGCTTTATCTTGATTTTCTTTGTATCTTTTGTTTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATAtt        <  2:31076/141‑1 (MQ=255)
                                                                                                                                       ttgttCGTCATGGTCTATGTAAATTTCTAGCGTCCCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATAtt        <  2:702485/138‑1 (MQ=255)
                                                                                                                                            cGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGc   >  1:487486/1‑141 (MQ=255)
                                                                                                                                             |                                                                                                                                            
CTTGTAGGATGTTAGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTATTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGCT  >  CP000730/2113159‑2113439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: