BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,113,305	G→T	7.1%	D36E (GAC→GAA)	USA300HOU_1981 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,113,305	0	G	T	7.1%	707.7 / 5.8	239	D36E (GAC→GAA)	USA300HOU_1981	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (119/97); new base T (21/2); total (140/99)
Fisher's exact test for biased strand distribution p-value = 5.80e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 242 total aligned reads displayed.

GGATGTTAGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGCTCTCT  >  CP000730/2113165‑2113443

ggATGTTAGTCCTTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATgg                                                                                                                                            <  2:513779/141‑1 (MQ=255)

  aTGTTAGTCACTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTc                                                                                                                                          <  2:869460/141‑1 (MQ=255)

   tGTTAGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGtgg                                                                                                                                         >  1:327036/1‑139 (MQ=255)

     ttatctaTTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGAt                                                                                                                                       <  2:379366/135‑1 (MQ=255)

        gTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTTTTCTTTTTCGATGTa                                                                                                                                    >  2:881674/1‑141 (MQ=255)

        gTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTa                                                                                                                                    >  1:723595/1‑141 (MQ=255)

          cATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTaaa                                                                                                                                  >  2:1084129/1‑141 (MQ=255)

          cATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTaaa                                                                                                                                  <  2:492377/141‑1 (MQ=255)

            ttttCCTTGCCCTCCCCATCAATTCCAGCCAATTCCTGAATAACGCTCTCCATCCTTTTATCTTTTAGATCTTCTGATACGAATACCTTTAAATCATTGAATTCTGTAATTA‑TTTTTTTTTCGTCATGGTCGATGTAAAtt                                                                                                                                <  2:299395/141‑1 (MQ=255)

             tttCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATACCGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAAttt                                                                                                                               <  2:603576/141‑1 (MQ=255)

               tCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCt                                                                                                                             <  1:591816/141‑1 (MQ=255)

                ccTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTa                                                                                                                            >  1:68963/1‑141 (MQ=255)

                 cTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTACTTA‑TTGTTTTTTCGTCATGGTCGATGTGAATCTATAg                                                                                                                           >  1:135740/1‑141 (MQ=255)

                 cTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAg                                                                                                                           <  1:623988/141‑1 (MQ=255)

                 cTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAg                                                                                                                           >  2:749347/1‑141 (MQ=255)

                 cTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAg                                                                                                                           >  1:407224/1‑141 (MQ=255)

                    gtacttctcATCATTCCCTGCCAATTCATACATATCGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGt                                                                                                                        <  2:1041991/135‑1 (MQ=255)

                    ggcctcctCATCAAGTCCAGCCAATTCATACATACCACCGTCCATACTATGTTTTGTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGt                                                                                                                        <  2:387360/139‑1 (MQ=255)

                     tcctccTCATCTATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTa                                                                                                                       <  2:1024632/141‑1 (MQ=255)

                     tcctccTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAGATTTCTAGCGTa                                                                                                                       <  2:534079/141‑1 (MQ=255)

                             atcaATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGc                                                                                                               >  1:435802/1‑141 (MQ=255)

                               caATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTa                                                                                                             <  1:1074977/141‑1 (MQ=255)

                                aaTTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTaa                                                                                                            >  1:344442/1‑141 (MQ=255)

                                   tCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAAtt                                                                                                         <  2:161546/141‑1 (MQ=255)

                                      cgCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTaaa                                                                                                      <  1:118236/140‑1 (MQ=255)

                                      aGCCAATTCATACATAACGATCTCCATTGTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTaaa                                                                                                      <  1:197728/141‑1 (MQ=255)

                                       gCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTaaaa                                                                                                     >  1:901379/1‑141 (MQ=255)

                                         cAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTCCAATCAGATGAATTaaaacc                                                                                                   <  2:1073966/141‑3 (MQ=255)

                                            ttCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTACTTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTATATTCAAAATAga                                                                                                >  2:865371/1‑140 (MQ=255)

                                              catacataACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCtt                                                                                              <  1:769320/141‑1 (MQ=255)

                                               ctacataACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCttt                                                                                             <  1:881307/140‑1 (MQ=255)

                                               agacataACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTGCTAGCGTACGATCAGCTAAATTAGCAATAGCttt                                                                                             <  1:810118/139‑1 (MQ=255)

                                                     aaCGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTc                                                                                       <  1:64580/141‑1 (MQ=255)

                                                      aCGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCa                                                                                      <  1:578953/141‑1 (MQ=255)

                                                          tctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCAtttt                                                                                  >  1:876404/1‑141 (MQ=255)

                                                          tctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCAtttt                                                                                  >  1:872608/1‑141 (MQ=255)

                                                          tctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCAtttt                                                                                  >  2:972846/1‑141 (MQ=255)

                                                           ctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTTTTTTTTTTTTCTGTAAATTTCTAGCGTACCATCAGTTAACTTAGAAATAGCTTTATCTCCGttttt                                                                                 >  2:781958/1‑140 (MQ=255)

                                                            tcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCt                                                                                >  1:19255/1‑141 (MQ=255)

                                                             ccATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCtt                                                                               <  2:819638/141‑1 (MQ=255)

                                                              cATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCttt                                                                              >  1:350783/1‑141 (MQ=255)

                                                              cATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCttt                                                                              >  1:464417/1‑141 (MQ=255)

                                                              cATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCttt                                                                              >  1:931966/1‑141 (MQ=255)

                                                                tACTTTTATCTTTTAAATATTCTGAAACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTAGTCATGTTCGCTGTAAATTTATAGCGAACCATCAACTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGt                                                                            >  2:41683/1‑141 (MQ=255)

                                                                     ttATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTCTGTATCCt                                                                       >  1:560420/1‑141 (MQ=255)

                                                                        tCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATTTTCATTTTCTTTGTATCCtttt                                                                    >  2:706731/1‑141 (MQ=255)

                                                                           tttAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTTGTTTTTTTTGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCt                                                                 >  2:732975/1‑141 (MQ=255)

                                                                            ttAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCGTGGTCGATGTACAGTTCTAGCGTACCATCAGCTAAATTAGAAATAGCTTTATCTTCATTTTCTTTGTATCCATTTTCtt                                                                >  2:903364/1‑141 (MQ=255)

                                                                            ttAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCtt                                                                >  1:292469/1‑141 (MQ=255)

                                                                            ttAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTGATCTTCCTTTTCTTTGTATCCTTTTTCtt                                                                >  1:385910/1‑141 (MQ=255)

                                                                             tAAATCTTCTGACACGAATACCTTTAAATCAGGGAATTCT‑AATTCACCTACTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCttt                                                               <  1:520905/141‑1 (MQ=255)

                                                                              aaaTCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCtttt                                                              >  1:299691/1‑141 (MQ=255)

                                                                              aaaTCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGAACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCtttt                                                              <  1:664744/141‑1 (MQ=255)

                                                                                    tctGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTTTTTTTTTTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAttttt                                                        >  2:554383/1‑140 (MQ=255)

                                                                                     ctGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATttct                                                       <  1:141997/141‑1 (MQ=255)

                                                                                       gACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTTTTTTTTTTCTATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATttcttc                                                     >  2:281210/1‑141 (MQ=255)

                                                                                       gACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATttcttc                                                     >  1:495368/1‑141 (MQ=255)

                                                                                        acacGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTACTTTCTTCa                                                    >  1:83238/1‑141 (MQ=255)

                                                                                         cacGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCaa                                                   >  2:943791/1‑141 (MQ=255)

                                                                                           cGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAg                                                 >  1:970098/1‑141 (MQ=255)

                                                                                                aCCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTTTTCAAAGATTCa                                            >  2:13921/1‑141 (MQ=255)

                                                                                                aCCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTAATGGTCAATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCa                                            <  2:501440/141‑1 (MQ=255)

                                                                                                 ccTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTTTTTTTTTTTCTTCAATGATTcct                                           >  2:985673/1‑139 (MQ=255)

                                                                                                 ccTTTAAATCAGGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAGATTTCTAGCGTACCATCAGCTGAATTAAAAGTAGCTTTATCATCATGTTCTTTGTGTCCTTTTTCTTTTAGTTTCTTCACAGATGCAt                                           >  2:24471/1‑141 (MQ=255)

                                                                                                   tttAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTa                                         <  2:635030/141‑1 (MQ=255)

                                                                                                   tttAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAACTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTa                                         >  2:124607/1‑141 (MQ=255)

                                                                                                    ttAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCAATACCGTTGTATCCTTTTTTTTTTAATTTTTTCAAAGATTCATTaa                                        >  2:433739/1‑141 (MQ=255)

                                                                                                     tAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTCCCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTCAATTTCTTCAAAGATTCATTAat                                       <  2:792079/141‑1 (MQ=255)

                                                                                                       aaTCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAattt                                     <  2:456933/141‑1 (MQ=255)

                                                                                                        aTCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCGTTTTCTTTGTATCCTTTTTCTTTTCATTGCTTCAAAGATTCATTAttttt                                    >  1:454462/1‑141 (MQ=255)

                                                                                                         tCATGGAATTCTGTAATTA‑TTGTTTTTTTTTTTTTTTTTATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTTTTTTAATTTCTTCAAAGTTTTATTAtttttt                                   >  2:680364/1‑140 (MQ=255)

                                                                                                            tGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTCATTTCTTCAAAGACTCAttctttttcttt                                >  1:321690/1‑141 (MQ=255)

                                                                                                            tGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAattttcatt                                >  1:961568/1‑141 (MQ=255)

                                                                                                            tGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAattttcatt                                >  2:506039/1‑141 (MQ=255)

                                                                                                            tGGAATTCTGTAATTA‑TTGATTTCTGGGGGGTGCCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCATTTTTCTTTTAATTTCTTCAAAGATTCATTAattttcatt                                >  1:534618/1‑141 (MQ=255)

                                                                                                             ggAATTCTGTAATTA‑TTGTTTTTTCGTCATTGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTTTTTTATTTTCTTTAAAGATTCATTAgttttctttt                               >  2:829047/1‑141 (MQ=255)

                                                                                                                aTTCTGTAATTA‑TTGTTTTTTTTTTTTTTTTGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAATGATTTATTTatttttattttct                            >  2:677443/1‑140 (MQ=255)

                                                                                                                aTTCTGGAATTA‑TTGTTTTTTCTTCTTGTTCGAAGTAAATTTCTAGTGTACCATCAGCTCAATTAAAAATAGCTTTCTTTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCCTTATTTTTCATTTTcc                            <  2:898571/141‑1 (MQ=255)

                                                                                                                 ttCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCt                           <  1:609144/141‑1 (MQ=255)

                                                                                                                  tCTGTAATTA‑TTTGTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTg                          <  2:20146/141‑1 (MQ=255)

                                                                                                                   ttGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCCTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGt                         <  2:185155/140‑1 (MQ=255)

                                                                                                                   cTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTACATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGt                         <  2:597133/141‑1 (MQ=255)

                                                                                                                      tAATTA‑TTGTTTTTTTTTTATTTTTTATGTAAATTACTAGCGTACCATCAGCTAAATTAGAATTAGCTTTATCTTCATTTTCTTTGTATCCTTTTTTTTTTAATTTACTCAATGATTCATTACTTTTCATTTTCCTGTtcc                      >  2:618784/1‑141 (MQ=255)

                                                                                                                      tAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTtcc                      >  2:652386/1‑141 (MQ=255)

                                                                                                                        atta‑ctGTTGTCTCGTCATGGTCGATGTAAATTTCTAGCGTACCATAAAATAAATAAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTtcctc                    <  2:365996/141‑1 (MQ=255)

                                                                                                                          tttgtgctttttCGTTATGGTGGATGTAAATTTCGTGCGTACCCTAGGCTAAATTAAAGATAGGTTTGTCATCATTTTCTTTGTATCCTTTTTCTCTTAATTTCTTCAAAGATTCATTAAGTTTCACTTTCGTGTgcctct                   <  2:330934/134‑2 (MQ=255)

                                                                                                                          ta‑ttGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTcctcct                  <  1:635126/141‑1 (MQ=255)

                                                                                                                                ttttttCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCatat             <  1:1093875/141‑1 (MQ=255)

                                                                                                                                 tttttCTTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTACATTAAAAATAGCTTTATCTTGATTTTCTTTGTATCTTTTGTTTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATAtt            <  2:31076/141‑1 (MQ=255)

                                                                                                                                 tttttCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCGTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATAtt            <  2:945380/141‑1 (MQ=255)

                                                                                                                                  ttttCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTc           <  2:153948/141‑1 (MQ=255)

                                                                                                                                   tttCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCa          <  1:887134/141‑1 (MQ=255)

                                                                                                                                      cGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGc       >  1:487486/1‑141 (MQ=255)

                                                                                                                                       gTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTccatct      >  1:236866/1‑136 (MQ=255)

                                                                                                                                       gTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGct      <  1:426744/141‑1 (MQ=255)

                                                                                                                                       gTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGct      <  2:482974/141‑1 (MQ=255)

                                                                                                                                         cATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGctct    >  1:9954/1‑141 (MQ=255)

                                                                                                                                         cATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCATCATATTCAAActct    >  1:670581/1‑141 (MQ=255)

                                                                                                                                         cATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTATGGTGAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCATGctct    >  1:804565/1‑141 (MQ=255)

                                                                                                                                           tGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGctctct  >  1:923808/1‑141 (MQ=255)

GGATGTTAGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGCTCTCT  >  CP000730/2113165‑2113443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`