Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,489,727 C→A 7.4% intergenic (+76/+77) USA300HOU_2350 → / ← USA300HOU_2351 hypothetical protein/hypothetical lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,489,7270CA 7.4% 277.5 / 2.2 93intergenic (+76/+77)USA300HOU_2350/USA300HOU_2351hypothetical protein/hypothetical lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (35/49);  new base A (2/7);  total (37/56)
Fisher's exact test for biased strand distribution p-value = 3.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACAACCATTAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGTAACCATTGCTTGTCCAGTATAATCAACCGTTACAAT  >  CP000730/2489592‑2489863
                                                                                                                                       |                                                                                                                                        
gAACAACCATTAGAATCTATTTTGTTACGGATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCaaaaa                                                                                                                                     >  2:508072/1‑141 (MQ=255)
gAACAACCATTAGAATCTATTTCAGAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCaaaaa                                                                                                                                     >  1:708836/1‑141 (MQ=255)
gAACAACCATTAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATACAACGTAAATTTTGATACTTTAAAATACGCaaaaa                                                                                                                                     >  2:52060/1‑141 (MQ=255)
gAACAACCATTAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAACCTTTGATACTTTAAAATACGCaaaaa                                                                                                                                     >  2:662458/1‑141 (MQ=255)
gAACAACCATTAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATTCTTTAAAATTCGCaaaaa                                                                                                                                     >  2:137914/1‑141 (MQ=255)
gAACAACCATTAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCGGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCaaaaa                                                                                                                                     >  2:135205/1‑141 (MQ=255)
aaacaacCATCAGCATCTACTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATATAACGTAAACTTTGATACTTTAAAATACGCaaaaa                                                                                                                                     <  1:587351/140‑1 (MQ=255)
 aacaacCATTAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCaaaaaa                                                                                                                                    <  2:823743/141‑1 (MQ=255)
    aacCATTAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAATCGTAATCTTTGATACTTTAAAATACGCAAAAAACgg                                                                                                                                 <  2:249351/141‑1 (MQ=255)
    aacCATTAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACgg                                                                                                                                 <  1:394221/141‑1 (MQ=255)
    aaacATGAGAAACTATTTCACAACTTAAAAAGAATAAAATAACTTCTAATCAATAATGTTTAAATTTCCAAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTCAACTTTGATACTTAAAAAAAAAAAAAAAACgg                                                                                                                                 <  2:637721/138‑1 (MQ=255)
      ccATTAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGTTa                                                                                                                               >  2:181445/1‑141 (MQ=255)
      ccATTAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTa                                                                                                                               >  2:440772/1‑141 (MQ=255)
       cATTAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTaa                                                                                                                              >  2:800961/1‑141 (MQ=255)
       cATTAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTaa                                                                                                                              >  2:223493/1‑141 (MQ=255)
         ttAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAACCTTTGATACTTTAAAATACGATAAACACGGTAAAc                                                                                                                            >  1:633823/1‑141 (MQ=255)
          tAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAAcc                                                                                                                           <  1:1005917/141‑1 (MQ=255)
           acaaTCGATTTAAAAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAAccc                                                                                                                          <  2:599936/139‑1 (MQ=255)
           aGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAAccc                                                                                                                          <  2:457484/141‑1 (MQ=255)
             aaacaaattCACAAATTACATTGTCTAAGATGACATCTAATCAATAGTGTTGAAATTTCTCAGGGGCAGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATAATAAAAAAAAAAAAAAAAACGGTAAACCCTa                                                                                                                        <  1:141040/134‑1 (MQ=255)
                 tATTTCACAACTTATATTGTCTAAGTTGACATCTAATCAATAGAGGTTAAATTTCTCTGTGGCTGTGACTGAGGATTAAAAGTACTATATGATGTAAACTTTGATACTTTAGAATACGCAAAAAACGGCAAACCCGGATTc                                                                                                                    >  2:365236/1‑141 (MQ=255)
                   tttCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCat                                                                                                                  >  1:662233/1‑141 (MQ=255)
                        aaaCTTGTATTGTCTAAGGAGAAATATAATAAATATTGTTTAATGTTCTCAGTTGCTGTGCATAAGATTTACCAGTACTACTAGACGGAAACTTTGATACTTAAAAAAACGCAAAAAACGGTAAACCCTAATTCAtattat                                                                                                             <  2:333384/140‑1 (MQ=255)
                         aaCTTATATTGGCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAACCCCTAATTCATATtata                                                                                                            <  1:588983/141‑1 (MQ=255)
                          aCTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATag                                                                                                           >  1:928971/1‑141 (MQ=255)
                                    tttcagtggcaatttAATCAATAGTTTTTAAAGTTCACAAGAGCTCGGACGAAGATTCAAAAGTACTATAAAACGGAAACTTTAATAAATAAAAAAAAAAAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGt                                                                                                 <  2:748379/127‑1 (MQ=255)
                                    tCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGt                                                                                                 <  1:221541/141‑1 (MQ=255)
                                       aaGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGtttt                                                                                              <  1:680327/141‑1 (MQ=255)
                                       aaGATGACATCTAATCAAAAGTGTTTAAATTTAACAGTGGCTAGCAATAAGGTTTAAAAGTACTATAAAACGTAAAACTTGAAACTAAAAAAAAAACAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGtttt                                                                                              <  2:477313/141‑1 (MQ=255)
                                          aTGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTAtt                                                                                           >  1:1023326/1‑141 (MQ=255)
                                          aTGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTAtt                                                                                           >  2:202258/1‑141 (MQ=255)
                                             actttaaaTTAATAGTGTTGAAAATTCTCATTGTTTGTGAAATAGGTTTAAAAGTACTATAAAACGTACACTTTGATACATTAAAAAAAGAAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTAttttt                                                                                        <  2:919974/135‑1 (MQ=255)
                                             aCATTTAATCAATAGTATTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAGCCCTAATTCATATTATAGAGTTTACCGTTTTAttttt                                                                                        <  2:168891/141‑1 (MQ=255)
                                              cATCTAAGCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTAtttttt                                                                                       <  1:1013268/141‑1 (MQ=255)
                                               aTCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTa                                                                                      <  1:685838/141‑1 (MQ=255)
                                                tCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTaa                                                                                     >  2:626084/1‑141 (MQ=255)
                                                  tAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATTAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACt                                                                                   <  2:664724/141‑1 (MQ=255)
                                                  tAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACt                                                                                   <  2:12956/141‑1 (MQ=255)
                                                  tAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACt                                                                                   >  2:340506/1‑141 (MQ=255)
                                                  tAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACt                                                                                   <  2:870670/141‑1 (MQ=255)
                                                  tAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACt                                                                                   <  1:849603/141‑1 (MQ=255)
                                                  caaTCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACAGTTTTATTTTTTAACt                                                                                   >  2:459228/2‑141 (MQ=255)
                                                   aaTCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACtt                                                                                  <  1:1101933/141‑1 (MQ=255)
                                                   aaTCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACtt                                                                                  <  1:347448/141‑1 (MQ=255)
                                                     taaaTAGTGTTTAAATTTCTCAGTTGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAATCTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGc                                                                                <  2:851604/139‑1 (MQ=255)
                                                     tCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGc                                                                                <  2:833920/141‑1 (MQ=255)
                                                     tCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGAAAAAAACGGTAAACCCTAATTCATATTATAGAGTATACCGTTTTATTTTTTAACTTGc                                                                                >  2:700277/1‑141 (MQ=255)
                                                     tCAATAGTGTTTAAAATTCTCAGTGACTATTAAGAACGTTTAAAAGCAATTTAAAACGTAAACTTAGATACTAAAAAAAAAAAAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGc                                                                                <  2:18193/141‑1 (MQ=255)
                                                     ataaTAGTGTTTAAATTTCGCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGc                                                                                <  2:749653/139‑1 (MQ=255)
                                                     acAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGc                                                                                <  2:18100/140‑1 (MQ=255)
                                                     acAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGc                                                                                <  2:909331/140‑1 (MQ=255)
                                                     acAATAGTGTTTAAATTTCTCAGTGGCTGTGAAAGAGGTTAAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGAAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGc                                                                                <  2:371477/140‑1 (MQ=255)
                                                        aTAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGcatc                                                                             >  1:234340/1‑141 (MQ=255)
                                                           gtgtTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTCTAGAGTTTACCGTTTTATTTTTTAACTTCCATCATa                                                                          >  2:1025916/1‑141 (MQ=255)
                                                           gtgtTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTCTAGAGTTAACCGTTTTCTTTTTTAACTTGCTTCATa                                                                          >  2:1025977/1‑141 (MQ=255)
                                                           gtgtTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGcatcatt                                                                          >  2:991744/1‑140 (MQ=255)
                                                             gtTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGt                                                                        <  2:928971/141‑1 (MQ=255)
                                                             gtTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGt                                                                        <  1:285466/141‑1 (MQ=255)
                                                             gtTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTAGAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAATCCCGAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGt                                                                        <  1:472106/141‑1 (MQ=255)
                                                                 aaaTTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTtata                                                                    <  2:234340/141‑1 (MQ=255)
                                                                    tttCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTa                                                                 >  1:755003/1‑141 (MQ=255)
                                                                     ttCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTaa                                                                <  2:770512/141‑1 (MQ=255)
                                                                     ttCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTaa                                                                <  1:959335/141‑1 (MQ=255)
                                                                       ctcAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACa                                                              >  1:823382/1‑141 (MQ=255)
                                                                                 gtgAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTgg                                                    >  2:536805/1‑141 (MQ=255)
                                                                                    tattatgattAATATTACTATAAAAAGTAAACTTTGATACTTTAAATTACGCAATAAACCGTAATTCCTAACTCATATTATAAAAGTTACCGTTTTATTTTTTAACTTGCATCATATTTATATTACCATCATTGTTGGTAg                                                 <  2:297143/133‑1 (MQ=255)
                                                                                    aagtaGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATCCATATTATATAGTTTACCGTTTTATTTTTTAACTTGCAACATAGTTATATTAACATTATTGTTGGTAg                                                 <  2:150117/137‑1 (MQ=255)
                                                                                    aaTGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAg                                                 <  2:18618/141‑1 (MQ=255)
                                                                                      agAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTATTTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGtt                                               <  2:378134/140‑1 (MQ=255)
                                                                                       gAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGttt                                              >  1:1000576/1‑141 (MQ=255)
                                                                                       gAGGTTTAAAAGTACGATAAAACGTCAACTATGATACTTTAAAATACGCAAAAAACGGAAAACCCTAATTCGTAATAAAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGttt                                              >  2:514060/1‑141 (MQ=255)
                                                                                          ggttAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGa                                           <  1:793873/139‑1 (MQ=255)
                                                                                          gTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGa                                           <  1:135205/141‑1 (MQ=255)
                                                                                          gTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTATTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGa                                           <  2:708836/141‑1 (MQ=255)
                                                                                          gTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATGATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGa                                           <  1:507688/141‑1 (MQ=255)
                                                                                           tttAAAAGTACTATACAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAACTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGAt                                          >  1:824809/1‑141 (MQ=255)
                                                                                             tAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCa                                        >  2:828907/1‑141 (MQ=255)
                                                                                               aaaGTACTATAACACGTAAACTTTGATACTTTAAAATACGCCAAAAACGGTATACCCTAATTCATATTATAGAGTTTACCGCTTTATATTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGt                                      <  2:423797/141‑1 (MQ=255)
                                                                                               aaaGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGt                                      <  2:977912/141‑1 (MQ=255)
                                                                                               aaaGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGt                                      <  1:20062/141‑1 (MQ=255)
                                                                                                aaGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGTa                                     >  2:820170/1‑141 (MQ=255)
                                                                                                aaGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGTa                                     >  1:387102/1‑141 (MQ=255)
                                                                                                 aGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGTaa                                    >  2:281400/1‑141 (MQ=255)
                                                                                                       ataAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGTAACCATTg                              <  1:828907/141‑1 (MQ=255)
                                                                                                         aataCGTAAACTTTGATACTATAAAATAAGAAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGTAACCATTGCt                            <  2:797555/138‑1 (MQ=255)
                                                                                                           aaCGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGTAACCATTGCTTg                          <  2:198024/141‑1 (MQ=255)
                                                                                                           aaCGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGTAACCATTGCTTg                          <  2:895805/141‑1 (MQ=255)
                                                                                                                 aaCTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAAGTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGTAACCATTGCTTGTCAAGt                    >  2:1037919/1‑141 (MQ=255)
                                                                                                                  aCTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGTAACCATTGCTTGTCCAGta                   >  1:621965/1‑141 (MQ=255)
                                                                                                                    tttGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGTAACCATTGCTTGTCCAGtata                 <  1:223493/141‑1 (MQ=255)
                                                                                                                    tttGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGTAACCATTGCTTGTCCAGtata                 <  1:800961/141‑1 (MQ=255)
                                                                                                                      tGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGTAACCATTGCTTGTCCAGTATAAt               <  2:662233/141‑1 (MQ=255)
                                                                                                                                   tACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGTTAGTTTGGATCAGTAACCATTGCTTGTCCAGTATAATCAACCGTTACAAt  >  2:192791/1‑141 (MQ=255)
                                                                                                                                       |                                                                                                                                        
GAACAACCATTAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAGTGTTTAAATTTCTCAGTGGCTGTGAATGAGGTTTAAAAGTACTATAAAACGTAAACTTTGATACTTTAAAATACGCAAAAAACGGTAAACCCTAATTCATATTATAGAGTTTACCGTTTTATTTTTTAACTTGCATCATAGTTATATTAACATTATTGTTGGTAGTTTGGATCAGTAACCATTGCTTGTCCAGTATAATCAACCGTTACAAT  >  CP000730/2489592‑2489863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: