BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,658,932	G→A	5.1%	A119V (GCA→GTA)	yfkO ←	FMN reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,658,932	0	G	A	5.1%	352.3 / 2.6	115	A119V (GCA→GTA)	yfkO	FMN reductase
Reads supporting (aligned to +/- strand): ref base G (70/39); new base A (3/3); total (73/42)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 116 total aligned reads displayed.

CTTCCATCGGACATGAATCAATACCTAACAATGCGGCTGTCGTCATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGCACAAAATGGCTCGCTGTATCTAAT  >  CP000730/2658798‑2659072

cTTCCATCGGACATGAATCAATACCTAACAATGCGGCTGTCGTCATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGa                                                                                                                                        <  1:375833/141‑1 (MQ=255)

     aTCGGACATGAATCAATACCCGCAGCAGCTGGTGTCGTCATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAAtttt                                                                                                                                   >  1:1098364/1‑141 (MQ=255)

              gAATCAATACCTAACCATGCGGCTGTCGTCATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCATAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACa                                                                                                                          <  1:759314/141‑1 (MQ=255)

              gAATCAATACCTAACAATGCGGCTGTCGTCATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACa                                                                                                                          >  1:578671/1‑141 (MQ=255)

              gAATCAATACCTAACAATGCGGCTGTCGTCATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACa                                                                                                                          >  1:600738/1‑141 (MQ=255)

                 tCAATACCTAACAATGCGGCTGTCGTCATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCt                                                                                                                       <  1:1095813/141‑1 (MQ=255)

                 tCAATACCTAACAATGAGACTGTCGTCATCAAATTGCCTAATACCATATACGATTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAAAATGGAAATCTGCTTGGAATGCATCGAATAGTTGTTCAACAGCt                                                                                                                       >  2:858888/1‑141 (MQ=255)

                   aaTACCTAACAATGCGGCTGTCGGCATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTgg                                                                                                                     >  2:509180/1‑141 (MQ=255)

                       ccTAACAATGCGGCTGTCGTCATCGTATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTATTGTTCAACAGCTGGAATc                                                                                                                 >  1:702907/1‑141 (MQ=255)

                        cTAACAATGCGGCTGTCGTCATCATTTTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCg                                                                                                                <  1:675844/141‑1 (MQ=255)

                        cTAACAATGCGGCTGTCGTCATCATTTTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCg                                                                                                                <  2:1004009/141‑1 (MQ=255)

                        cTAACAATGCGGCTGTCGTCATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGCAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCg                                                                                                                <  1:860921/141‑1 (MQ=255)

                        cTAACAATGCGGCTGTCGTCATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCg                                                                                                                <  1:676400/141‑1 (MQ=255)

                          taCAATGCGTCTGTCGTCATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGtt                                                                                                              <  2:126471/140‑1 (MQ=255)

                          aaCAATGCGGCTGTCGTCATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGTTCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGCAtgatg                                                                                                              >  2:822690/1‑137 (MQ=255)

                              aTGCGGCTGTGGTCATCATATTGCCTAATGCGATATACGTTTGTTTAATTGGCGAGTCATGTAAGGGTTGATCTTTTTCAGAAGTATGGGAATTTGCTTGGGATGCATCGGATTTTTGTTCAAGAGGTGGATTGGGTtgtg                                                                                                          >  2:569338/1‑141 (MQ=255)

                                gCGGCTGTCGTCATCATATTGCCTAATGCCATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAACCGTTTGTGcc                                                                                                        >  2:10485/1‑141 (MQ=255)

                                gCGGCTGTCGTCATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTTTGcc                                                                                                        >  1:676848/1‑141 (MQ=255)

                                 cGGCTGTCGTCATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCt                                                                                                       <  2:651209/141‑1 (MQ=255)

                                  tgCTGTCTTCATCATATTGCCTAATTCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTc                                                                                                      <  1:147417/140‑1 (MQ=255)

                                  gtttGTCGTCTTCTTATTGTCTAATCAAATCTACGTTTGTTTACTTGACCAGTCATACACGGCTTGAGCATTATCAGAAATATGGTAATCTGCTTGGAATGCATCCAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTc                                                                                                      <  1:344481/138‑1 (MQ=255)

                                    cTGTCGTCATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCat                                                                                                    <  2:344354/141‑1 (MQ=255)

                                       tcgtcATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATAtt                                                                                                 >  1:109995/1‑141 (MQ=255)

                                       tcgtcATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATAtt                                                                                                 >  1:930212/1‑141 (MQ=255)

                                       tcgtcATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATAtt                                                                                                 >  1:377182/1‑141 (MQ=255)

                                          tcatcaTATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCCTAttttt                                                                                              >  1:491764/1‑141 (MQ=255)

                                            tgcaagttGCCTTATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTa                                                                                            <  1:246161/135‑1 (MQ=255)

                                            atcatATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATCTTTTTTa                                                                                            >  1:327760/1‑141 (MQ=255)

                                            atcatATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTa                                                                                            >  2:788685/1‑141 (MQ=255)

                                            atcatATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTa                                                                                            >  1:103565/1‑141 (MQ=255)

                                                 atTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATAtc                                                                                       <  1:874078/141‑1 (MQ=255)

                                                 atTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATAtc                                                                                       <  2:628286/141‑1 (MQ=255)

                                                   tGCCTAATGGAGTATACGTTTGTTTACTTGACCAGTCTTACAAGGCTTGATTATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGTATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTGGTAtctc                                                                                     <  2:957130/141‑1 (MQ=255)

                                                   tGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATAtctc                                                                                     >  2:974288/1‑141 (MQ=255)

                                                         aTGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGTAATATGGAAATCTGCTTGGAATACATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACa                                                                               >  1:717091/1‑141 (MQ=255)

                                                         aTGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATACATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACa                                                                               >  1:518897/1‑141 (MQ=255)

                                                         aTGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATACATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACa                                                                               >  1:1068090/1‑141 (MQ=255)

                                                              atatACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATAtgt                                                                          >  1:824270/1‑141 (MQ=255)

                                                              atatACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATAtgt                                                                          >  1:887809/1‑141 (MQ=255)

                                                              atatACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATAtgt                                                                          >  2:292730/1‑141 (MQ=255)

                                                              atatACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATAtgt                                                                          >  2:402084/1‑141 (MQ=255)

                                                              atatACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATAGGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATAtgt                                                                          >  2:280714/1‑141 (MQ=255)

                                                                ataCGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGACTCATATTTTTTACTCTCTCTTAACATAtgttg                                                                        >  1:53275/1‑141 (MQ=255)

                                                                ataCGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATGATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATAtgttg                                                                        >  1:755952/1‑141 (MQ=255)

                                                                   cGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTGCATTTCTTTTACCTTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTAc                                                                     >  1:306608/1‑141 (MQ=255)

                                                                    gtttgtttACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACa                                                                    >  1:826837/1‑141 (MQ=255)

                                                                     tttgtttACTTGACCAGTCATACAAGGCTTGATCAATAACAGAAATATGGGAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACAt                                                                   <  1:301769/141‑1 (MQ=255)

                                                                     tttgtttACTTGACCAGTCATACAAGGCGTGATAATTATCAAAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACAt                                                                   <  1:301629/141‑1 (MQ=255)

                                                                         tttACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGGTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACgg                                                               <  2:203436/141‑1 (MQ=255)

                                                                          ttACTTGACCAGTCATACAAGGCTTGATCATTATTCAACGAAAGAGAATCTGGTCATTATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTGGTCCATACgtt                                                              >  1:259912/1‑139 (MQ=255)

                                                                          ttACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGt                                                              <  2:600738/141‑1 (MQ=255)

                                                                          ttACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGt                                                              <  2:578671/141‑1 (MQ=255)

                                                                           taattGACCAGTCACACAAGGCTTGATCATTATTAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTTTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTg                                                             <  2:55937/138‑1 (MQ=255)

                                                                            aCTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGa                                                            >  1:607595/1‑141 (MQ=255)

                                                                             cTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAGTGCAGCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTCCATACGGTGAt                                                           >  1:165268/1‑141 (MQ=255)

                                                                              ttGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATc                                                          >  1:733279/1‑141 (MQ=255)

                                                                              ttGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATc                                                          >  2:709047/1‑141 (MQ=255)

                                                                                  ccAGTCATACAAGGCTTGATCATTATCAGAACTATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACCGCTGGGATCGTTTGTGCCTCATATTTTTTAATATCTCTTACCATATGTTGTACATACGGTGATCTTGa                                                      >  2:117800/1‑141 (MQ=255)

                                                                                  ccAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGa                                                      >  2:170181/1‑141 (MQ=255)

                                                                                   cattCATACGAGTCTTGATCATTATCAGAAAAATGGAAATCTGCTTGGAATGCACCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGAc                                                     <  1:227515/138‑1 (MQ=255)

                                                                                     gTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGt                                                   >  2:929612/1‑141 (MQ=255)

                                                                                     gTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGt                                                   >  1:196809/1‑141 (MQ=255)

                                                                                     gTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATATTGACGt                                                   >  2:931342/1‑141 (MQ=255)

                                                                                      tCATAAAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTgtggtt                                                  >  2:1084829/1‑136 (MQ=255)

                                                                                        ctagcaGGCTTGATCATTATCAGAATTATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACAGACGGTGATCTTGACGTTAc                                                <  2:1071050/136‑1 (MQ=255)

                                                                                          aCAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACAt                                              <  1:508399/141‑1 (MQ=255)

                                                                                          aCAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACAt                                              >  1:961953/1‑141 (MQ=255)

                                                                                           aaaGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACAtt                                             <  1:735152/140‑1 (MQ=255)

                                                                                             aGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCCCTTAACATATGTTGTACATACGGTGATCTTGACGTTACAtttt                                           >  2:345110/1‑141 (MQ=255)

                                                                                                 ttGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTAcg                                       >  1:768995/1‑141 (MQ=255)

                                                                                                    agcATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGACCTTGACGTTACATTTTTACgcgc                                    >  2:1072643/3‑141 (MQ=255)

                                                                                                    aTCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACgcgc                                    >  2:638466/1‑141 (MQ=255)

                                                                                                    aTCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACgagc                                    >  2:137577/1‑141 (MQ=255)

                                                                                                     tCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCa                                   >  1:1086102/1‑141 (MQ=255)

                                                                                                        ttATCAGAAATATGGAAATCTTCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTAACGTTACATTATTACGCGCgaaa                                >  1:927499/1‑141 (MQ=255)

                                                                                                        ttATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCaaaa                                >  1:543934/1‑141 (MQ=255)

                                                                                                        ttATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGAGCTTGACGTTACATTTTTACGCGCaaaa                                >  1:515756/1‑141 (MQ=255)

                                                                                                        taaacAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGATTGTGCCTCATATTTTTTAATGTCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACTCGCaaaa                                >  2:566921/5‑141 (MQ=255)

                                                                                                         tATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGCCGTTACATTTTTACGCGCaaaaa                               >  1:299818/1‑141 (MQ=255)

                                                                                                         tATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCaaaaa                               >  1:300187/1‑141 (MQ=255)

                                                                                                          aTCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAAt                              >  2:733643/1‑141 (MQ=255)

                                                                                                                aaTATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACTCGCAAAAATTAGcac                        <  1:822690/141‑1 (MQ=255)

                                                                                                                aaTATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCGTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGcac                        <  1:1094715/141‑1 (MQ=255)

                                                                                                                    tGGAAATCTGCTTGGAATGCATCGAATTTTTCTCCAACAGCTGGAACCGCATGAGCCTCATATTTTTTAATATCTATTATAATACGTGGCACATACGATGATCTTGTCTTTACATTTTTACTCGCAAAAATTAGCACaaaa                    >  2:809691/1‑141 (MQ=255)

                                                                                                                     ggAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGCACAAAAt                   <  1:8957/141‑1 (MQ=255)

                                                                                                                      gAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGCACAAAATg                  <  2:962378/141‑1 (MQ=255)

                                                                                                                       aaaTCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGCACAAAATgg                 >  1:346189/1‑141 (MQ=255)

                                                                                                                        aaccTGCTTGGAATACATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACAATTTTTTGCTCAATAATTAGCACAAAATGGc                <  2:1068090/138‑1 (MQ=255)

                                                                                                                        aaTCTGCTTGGAATACATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGCACAAAATGGc                <  2:717091/141‑1 (MQ=255)

                                                                                                                        aaTCTGCTTGGAATACATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGCACAAAATGGc                <  2:518897/141‑1 (MQ=255)

                                                                                                                            tGCTTGGAATGCCTCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGCACAAAATGGCTCGc            <  1:901680/141‑1 (MQ=255)

                                                                                                                              cTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGCACAAAATGGCTCGCtt          >  2:108476/1‑140 (MQ=255)

                                                                                                                              cTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGCACAAAATGGCTCGCTg          >  2:178801/1‑141 (MQ=255)

                                                                                                                              cTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGCACAAAATGGATAGCTg          >  2:601171/1‑141 (MQ=255)

                                                                                                                               ttGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGCACACAATGGATCGCTGt         >  2:261925/1‑141 (MQ=255)

                                                                                                                                tGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGCACAAAATGGCTCGCTgaa        >  2:339504/1‑139 (MQ=255)

                                                                                                                                  gAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGCACAAAATGGCTCGCTGTATc      <  2:103565/141‑1 (MQ=255)

                                                                                                                                    aTGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGCACAAAATGGCTCGCTGTATCTa    <  2:768995/141‑1 (MQ=255)

                                                                                                                                     tGCATCGAATTTTTGTTCAAAAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTAGATTTTTACGCGCAAAAATTAGCACAAAATGGCTCGCTGTATCTac   >  1:777037/1‑140 (MQ=255)

                                                                                                                                      gCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGCACAAAATGGCTCGCTGTATCTAAt  <  1:712001/141‑1 (MQ=255)

CTTCCATCGGACATGAATCAATACCTAACAATGCGGCTGTCGTCATCATATTGCCTAATGCAATATACGTTTGTTTACTTGACCAGTCATACAAGGCTTGATCATTATCAGAAATATGGAAATCTGCTTGGAATGCATCGAATTTTTGTTCAACAGCTGGAATCGTTTGTGCCTCATATTTTTTAATATCTCTTAACATATGTTGTACATACGGTGATCTTGACGTTACATTTTTACGCGCAAAAATTAGCACAAAATGGCTCGCTGTATCTAAT  >  CP000730/2658798‑2659072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`